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Microalgae As Solar-Powered Protein Factories.

F. Hempel, U. G. Maier
Published 2016 · Medicine, Biology

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Microalgae have an enormous ecological relevance as they contribute significantly to global carbon fixation. But also for biotechnology microalgae became increasingly interesting during the last decades as many algae provide valuable natural products. Especially the high lipid content of some species currently attracts much attention in the biodiesel industry. A further application that emerged some years ago is the use of microalgae as expression platform for recombinant proteins. Several projects on the production of therapeutics, vaccines and feed supplements demonstrated the great potential of using microalgae as novel low-cost expression platform. This review provides an overview on the prospects and advantages of microalgal protein expression systems and gives an outlook on potential future applications.
This paper references
10.1007/s1012601-0070-x
Stable Integration and Functional Expression of Flounder Growth Hormone Gene in Transformed Microalga, Chlorella ellipsoidea
D. Kim (2002)
10.1128/MCB.11.4.2328
Introduction of exogenous DNA into Chlamydomonas reinhardtii by electroporation.
L. E. Brown (1991)
10.1073/pnas.1105861108
High-efficiency homologous recombination in the oil-producing alga Nannochloropsis sp.
O. Kilian (2011)
10.1007/s11120-014-9994-7
Photosynthetic biomanufacturing in green algae; production of recombinant proteins for industrial, nutritional, and medical uses
B. Rasala (2014)
10.1186/1475-2859-11-126
An engineered diatom acting like a plasma cell secreting human IgG antibodies with high efficiency
F. Hempel (2012)
10.1126/SCIENCE.1143609
The Chlamydomonas Genome Reveals the Evolution of Key Animal and Plant Functions
S. Merchant (2007)
10.1016/j.jbiotec.2009.12.006
Heat-stable oral alga-based vaccine protects mice from Staphylococcus aureus infection.
I. Dreesen (2010)
10.1016/j.biotechadv.2011.07.015
Post-translational modification of plant-made foreign proteins; glycosylation and beyond.
D. Webster (2012)
10.1111/J.1745-7270.2006.00148.X
Survival of human metallothionein-2 transplastomic Chlamydomonas reinhardtii to ultraviolet B exposure.
Y. Zhang (2006)
10.1126/science.2897716
Chloroplast transformation in Chlamydomonas with high velocity microprojectiles.
J. Boynton (1988)
10.1016/j.biotechadv.2011.01.002
Towards high-yield production of pharmaceutical proteins with plant cell suspension cultures.
J. Xu (2011)
10.1111/pbi.12244
Algal chloroplast produced camelid VH H antitoxins are capable of neutralizing botulinum neurotoxin.
Daniel J. Barrera (2015)
10.3233/HAB-2007-163-408
Cumulative updating of approved biopharmaceuticals.
E. Redwan (2007)
10.1007/s11120-004-2216-y
The Chlamydomonas genome reveals its secrets: chaperone genes and the potential roles of their gene products in the chloroplast
M. Schroda (2004)
10.1371/journal.pone.0028424
Algae as Protein Factories: Expression of a Human Antibody and the Respective Antigen in the Diatom Phaeodactylum tricornutum
F. Hempel (2011)
10.1023/B:JAPH.0000004298.89183.e5
Stable expression of hepatitis B surface antigen gene in Dunaliella salina (Chlorophyta)
Degui Geng (2004)
10.1016/j.biotechadv.2010.08.006
Strategies for high-level recombinant protein expression in transgenic microalgae: a review.
Gabriel Potvin (2010)
10.1073/pnas.87.3.1228
High-frequency nuclear transformation of Chlamydomonas reinhardtii.
K. Kindle (1990)
10.1128/AEM.00714-13
Alga-Produced Cholera Toxin-Pfs25 Fusion Proteins as Oral Vaccines
James A. Gregory (2013)
10.1038/nbt1252
Post-translational modifications in the context of therapeutic proteins
G. Walsh (2006)
10.1016/S0958-1669(97)80061-4
Recombinant proteins from transgenic plants.
Franken (1997)
10.1111/J.1529-8817.2006.00269.X
MOLECULAR GENETIC MANIPULATION OF THE DIATOM THALASSIOSIRA PSEUDONANA (BACILLARIOPHYCEAE)
N. Poulsen (2006)
10.1007/s10811-005-4783-5
Towards the development of a nuclear transformation system for Dunaliella tertiolecta
Tara L. Walker (2005)
10.1126/SCIENCE.1101156
The Genome of the Diatom Thalassiosira Pseudonana: Ecology, Evolution, and Metabolism
E. V. Armbrust (2004)
10.1105/tpc.110.076406
The Chlorella variabilis NC64A Genome Reveals Adaptation to Photosymbiosis, Coevolution with Viruses, and Cryptic Sex[C][W]
G. Blanc (2010)
10.1007/s00294-005-0042-1
RNA silencing in Chlamydomonas: mechanisms and tools
M. Schroda (2005)
10.1177/153567600701200105
Animal Cell Cultures: Risk Assessment and Biosafety Recommendations
K. Pauwels (2007)
10.1007/s10126-014-9570-3
Construction of Novel Chloroplast Expression Vector and Development of an Efficient Transformation System for the Diatom Phaeodactylum tricornutum
W. Xie (2014)
10.1074/mcp.M113.028191
Exploring the N-glycosylation Pathway in Chlamydomonas reinhardtii Unravels Novel Complex Structures*
Elodie Mathieu-Rivet (2013)
10.1046/J.1365-313X.2003.01965.X
Development of a luciferase reporter gene, luxCt, for Chlamydomonas reinhardtii chloroplast.
S. Mayfield (2004)
10.1073/pnas.0237108100
Expression and assembly of a fully active antibody in algae
S. Mayfield (2003)
10.1007/BF02172403
Stable nuclear transformation of the diatomPhaeodactylum tricornutum
K. Apt (2005)
10.1046/J.1365-313X.1998.00145.X
Efficient foreign gene expression in Chlamydomonas reinhardtii mediated by an endogenous intron
V. Lumbreras (1998)
10.1111/pbi.12254
Inactivation of Phaeodactylum tricornutum urease gene using transcription activator-like effector nuclease-based targeted mutagenesis.
P. D. Weyman (2015)
10.1007/s11033-008-9333-1
Improvement of efficiency of genetic transformation for Dunaliella salina by glass beads method
Shuying Feng (2008)
10.1016/J.ALGAL.2012.10.004
Production of recombinant enzymes in the marine alga Dunaliella tertiolecta
D. R. Georgianna (2013)
10.1046/J.1365-313X.1999.00526.X
A synthetic gene coding for the green fluorescent protein (GFP) is a versatile reporter in Chlamydomonas reinhardtii.
M. Fuhrmann (1999)
Establishment of a micro-particle bombardment transformation system for Dunaliella salina.
Congping Tan (2005)
10.1007/s11103-004-2150-6
Monitoring dynamic expression of nuclear genes in Chlamydomonas reinhardtii by using a synthetic luciferase reporter gene
M. Fuhrmann (2004)
10.1007/s00299-011-1186-8
Chlamydomonas reinhardtii as a viable platform for the production of recombinant proteins: current status and perspectives
S. Rosales-Mendoza (2011)
10.1186/1472-6750-8-87
A novel expression platform for the production of diabetes-associated autoantigen human glutamic acid decarboxylase (hGAD65)
Xiaofeng Wang (2008)
10.1126/science.1189003
An Outlook on Microalgal Biofuels
R. H. Wijffels (2010)
10.1002/j.1460-2075.1989.tb08426.x
The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus.
R. Debuchy (1989)
10.1007/s10811-012-9847-8
Expression of soybean Kunitz trypsin inhibitor gene SKTI in Dunaliella salina
Xiaojie Chai (2012)
10.1007/s11240-013-0388-x
Expression of an HBcAg-based antigen carrying angiotensin II in Chlamydomonas reinhardtii as a candidate hypertension vaccine
R. Soria-Guerra (2013)
10.1016/j.biologicals.2009.02.005
Factors effecting expression of vaccines in microalgae.
Raymond Surzycki (2009)
10.1385/MB:30:3:185
Expression of foreign genes in Dunaliella by electroporation
Y. Sun (2005)
10.1016/J.PBI.2004.01.012
Prospects for molecular farming in the green alga Chlamydomonas.
S. Franklin (2004)
10.1080/10408410802086783
A Perspective on the Biotechnological Potential of Microalgae
R. B. Raja (2008)
10.1371/journal.pone.0094028
Enhanced Genetic Tools for Engineering Multigene Traits into Green Algae
B. Rasala (2014)
10.1016/j.biotechadv.2012.01.019
Closed photobioreactors for production of microalgal biomasses.
B. Wang (2012)
10.1371/journal.pone.0037179
Algae-Produced Pfs25 Elicits Antibodies That Inhibit Malaria Transmission
James A. Gregory (2012)
10.1126/SCIENCE.278.5345.1954
Protein disulfide isomerase as a regulator of chloroplast translational activation.
J. Kim (1997)
10.1074/jbc.M110.175711
N-Glycans of Phaeodactylum tricornutum Diatom and Functional Characterization of Its N-Acetylglucosaminyltransferase I Enzyme*
Bérengère Baïet (2010)
10.1038/nbt.2437
What's fueling the biotech engine—2011 to 2012
S. Aggarwal (2012)
10.1016/j.pep.2010.07.015
Production of protein complexes via co-expression.
John J. Kerrigan (2011)
10.3389/fpls.2014.00359
Protein N-glycosylation in eukaryotic microalgae and its impact on the production of nuclear expressed biopharmaceuticals
Elodie Mathieu-Rivet (2014)
10.1111/j.1467-7652.2010.00503.x
Production of therapeutic proteins in algae, analysis of expression of seven human proteins in the chloroplast of Chlamydomonas reinhardtii.
B. Rasala (2010)
10.1111/j.1365-313X.2008.03746.x
Generation of Chlamydomonas strains that efficiently express nuclear transgenes.
Juliane Neupert (2009)
10.1073/pnas.1214638110
Production of unique immunotoxin cancer therapeutics in algal chloroplasts
M. Tran (2012)
10.1016/j.fsi.2008.07.004
Transgenic microalgae as a non-antibiotic bactericide producer to defend against bacterial pathogen infection in the fish digestive tract.
Si-shen Li (2009)
10.1073/pnas.0710412105
Engineering of mucin-type human glycoproteins in yeast cells
K. Amano (2008)
10.1016/J.PLANTSCI.2003.11.012
Genetic transformation of the green alga: Chlamydomonas reinhardtii by Agrobacterium tumefaciens
S. V. Kumar (2004)
10.1002/bit.24966
Production of anti‐cancer immunotoxins in algae: Ribosome inactivating proteins as fusion partners
M. Tran (2013)
10.1016/j.jbiotec.2012.10.010
Efficient recombinant protein production and secretion from nuclear transgenes in Chlamydomonas reinhardtii.
K. Lauersen (2013)
10.1016/J.COLSURFB.2006.10.042
Recombination and expression of classical swine fever virus (CSFV) structural protein E2 gene in Chlamydomonas reinhardtii chroloplasts.
D. He (2007)
10.1007/s00253-011-3279-2
Transgenic microalgae expressing Escherichia coli AppA phytase as feed additive to reduce phytate excretion in the manure of young broiler chicks
So-Mi Yoon (2011)
10.1007/s00294-008-0189-7
A codon-optimized luciferase from Gaussia princeps facilitates the in vivo monitoring of gene expression in the model alga Chlamydomonas reinhardtii
N. Shao (2008)
10.1038/nbt1027
Plant cell cultures for the production of recombinant proteins
S. Hellwig (2004)
10.1002/btpr.695
Optimization of a glycoengineered Pichia pastoris cultivation process for commercial antibody production
Jianxin Ye (2011)
10.1007/s002849900268
Stable Transformation of Chlorella: Rescue of Nitrate Reductase-Deficient Mutants with the Nitrate Reductase Gene
H. Dawson (1997)
10.1371/journal.pone.0061473
A Chlamydomonas-Derived Human Papillomavirus 16 E7 Vaccine Induces Specific Tumor Protection
O. Demurtas (2013)
10.1038/nrg1177
Genetic modification: The production of recombinant pharmaceutical proteins in plants
J. Ma (2003)
10.1016/j.tplants.2009.09.009
Plant-made vaccine antigens and biopharmaceuticals
H. Daniell (2009)
10.1111/tpj.12066
Nuclear gene targeting in Chlamydomonas using engineered zinc-finger nucleases.
I. Sizova (2013)
10.1007/s10295-011-1032-6
Microalgae as platforms for production of recombinant proteins and valuable compounds: progress and prospects
Y. Gong (2011)
10.3390/nu5030624
Selenoprotein-Transgenic Chlamydomonas reinhardtii
Qintang Hou (2013)
10.1016/J.RSER.2009.07.020
Microalgae for biodiesel production and other applications: A review
T. Mata (2010)
10.1038/81132
Transgenic plants as factories for biopharmaceuticals
G. Giddings (2000)
10.3389/fimmu.2013.00217
Expression of Recombinant Antibodies
A. Frenzel (2013)
10.1016/J.PBI.2004.01.007
Plant-based production of biopharmaceuticals.
R. Fischer (2004)
10.1007/s11427-008-0136-3
Expression and function analysis of the metallothionein-like (MT-like) gene from Festuca rubra in Chlamydomonas reinhardtii chloroplast
Sihai Han (2008)
10.1007/s10529-010-0326-5
Micro-algae come of age as a platform for recombinant protein production
Elizabeth A. Specht (2010)
10.1186/1475-2859-8-17
Microbial factories for recombinant pharmaceuticals
N. Ferrer-Miralles (2009)
10.1016/J.TIBTECH.2003.11.003
Transgenic microalgae as green cell-factories.
Rosa León-Bañares (2004)
10.1002/bit.25357
Production of recombinant proteins in microalgae at pilot greenhouse scale
Javier A. Gimpel (2015)
10.1271/bbb.120936
Highly Efficient Transformation of the Diatom Phaeodactylum tricornutum by Multi-Pulse Electroporation
M. Miyahara (2013)
10.1038/nature07410
The Phaeodactylum genome reveals the evolutionary history of diatom genomes
C. Bowler (2008)
10.1023/A:1024140114505
Foot-and-mouth disease virus VP1 protein fused with cholera toxin B subunit expressed in Chlamydomonas reinhardtii chloroplast
M. Sun (2004)
10.1039/c4fo00125g
Functional ingredients from microalgae.
S. Buono (2014)
10.1016/j.margen.2013.10.003
High-efficiency nuclear transformation of the diatom Phaeodactylum tricornutum by electroporation.
C. Zhang (2014)
10.1007/s00705-013-1856-7
Preparation of transgenic Dunaliella salina for immunization against white spot syndrome virus in crayfish
Shuying Feng (2013)
10.1038/nature11479
Exploiting diversity and synthetic biology for the production of algal biofuels
D. R. Georgianna (2012)
10.1038/ncomms1688
Draft genome sequence and genetic transformation of the oleaginous alga Nannochloropis gaditana
Randor Radakovits (2012)
10.1007/s002990050660
Electrotransformation of Chlorella vulgaris
K.-C. Chow (1999)
10.1186/1475-2859-10-81
Microalgae as bioreactors for bioplastic production
F. Hempel (2011)
10.1007/s10811-006-9148-1
Removal of nitrogen and phosphorus from wastewater using microalgae immobilized on twin layers: an experimental study
J. Shi (2006)
10.1016/j.copbio.2012.03.011
Pharmaceutical protein production by yeast: towards production of human blood proteins by microbial fermentation.
J. L. Martínez (2012)
10.1111/j.1742-4658.2005.04760.x
A new molecular tool for transgenic diatoms
N. Poulsen (2005)
10.1371/journal.pone.0015424
Engineering the Chloroplast Targeted Malarial Vaccine Antigens in Chlamydomonas Starch Granules
D. Dauvillée (2010)
10.1007/s002940100205
Highly efficient expression of rabbit neutrophil peptide-1 gene in Chlorella ellipsoidea cells
Y. Chen (2001)
10.1038/ncomms4831
Genome engineering empowers the diatom Phaeodactylum tricornutum for biotechnology.
Fayza Daboussi (2014)
10.1371/journal.pgen.1003064
Genome, Functional Gene Annotation, and Nuclear Transformation of the Heterokont Oleaginous Alga Nannochloropsis oceanica CCMP1779
Astrid Vieler (2012)
10.1371/journal.pone.0054966
A New Strategy to Produce a Defensin: Stable Production of Mutated NP-1 in Nitrate Reductase-Deficient Chlorella ellipsoidea
Li-li Bai (2013)
10.1007/978-1-4757-5983-9_39
Heavy Metal Binding Properties of Wild Type and Transgenic Algae ( Chlamydomonas sp.)
Xiao-Hua Cai (1998)
Development of oral vaccines for human use.
Fooks Ar (2000)
10.1002/biot.201100369
Mammalian cells as biopharmaceutical production hosts in the age of omics
Stefanie Dietmair (2012)
10.1002/bit.22446
Synthesis and assembly of a full‐length human monoclonal antibody in algal chloroplasts
M. Tran (2009)
10.1007/s00425-008-0879-x
Strategies to facilitate transgene expression in Chlamydomonas reinhardtii
Alke Eichler-Stahlberg (2008)
10.1007/s00253-010-2721-1
Engineering of glycosylation in yeast and other fungi: current state and perspectives
Karen De Pourcq (2010)
10.3389/fmicb.2014.00060
Algae-based oral recombinant vaccines
Elizabeth A. Specht (2014)
10.1093/nar/gkp448
Gene silencing in the marine diatom Phaeodactylum tricornutum
Valentina De Riso (2009)
10.1111/j.1529-8817.2008.00508.x
CONDITIONAL PRODUCTION OF A FUNCTIONAL FISH GROWTH HORMONE IN THE TRANSGENIC LINE OF NANNOCHLOROPSIS OCULATA (EUSTIGMATOPHYCEAE)(1).
H. L. Chen (2008)
10.1007/978-0-387-75532-8_9
Phycoremediation of heavy metals using transgenic microalgae.
S. Rajamani (2007)
10.1007/S11434-006-2041-0
Expression of human soluble TRAIL in Chlamydomonas reinhardtii chloroplast
Zongqi Yang (2006)
10.1016/j.drudis.2008.10.002
Plant-produced vaccines: promise and reality.
E. Rybicki (2009)
10.1038/nrmicro1087
The humanization of N-glycosylation pathways in yeast
S. Wildt (2005)
10.1007/PL00011773
Transformation of Nonselectable Reporter Genes in Marine Diatoms
A. Falciatore (1999)
10.1073/pnas.1321652111
Global evaluation of biofuel potential from microalgae
J. Moody (2014)
10.1111/j.1467-7652.2009.00497.x
Plant-specific glycosylation patterns in the context of therapeutic protein production.
V. Gomord (2010)
10.1371/journal.pone.0043349
Robust Expression and Secretion of Xylanase1 in Chlamydomonas reinhardtii by Fusion to a Selection Gene and Processing with the FMDV 2A Peptide
B. Rasala (2012)
10.1007/PL00006813
Expression of Human Growth Hormone by the Eukaryotic Alga, Chlorella
M. Hawkins (1999)
10.1007/s00253-012-4071-7
Heterologous expression of the C-terminal antigenic domain of the malaria vaccine candidate Pfs48/45 in the green algae Chlamydomonas reinhardtii
Carla S. Jones (2012)
10.1042/BJ3380553
Identification and characterization of a recombinant metallothionein protein from a marine alga, Fucus vesiculosus.
C. Morris (1999)
10.1007/s00299-005-0004-6
Microalgae as bioreactors
Tara L. Walker (2005)
10.1007/s00253-013-5477-6
Developing inexpensive malaria vaccines from plants and algae
J. A. Gregory (2013)



This paper is referenced by
10.1099/mic.0.000910
Multigenic engineering of the chloroplast genome in the green alga Chlamydomonas reinhardtii
Marco Larrea-Álvarez (2020)
10.3389/fbioe.2019.00383
Downstream Processing of Chlamydomonas reinhardtii TN72 for Recombinant Protein Recovery
Laura Stoffels (2019)
10.1111/tpj.14620
Multiple xylosyltransferases heterogeneously xylosylate protein N-linked glycans in Chlamydomonas reinhardtii.
Pierre-louis Lucas (2019)
10.1186/s40643-018-0199-3
Green microalgae biomolecule separations and recovery
Chelsea K. Dixon (2018)
10.3390/biology7040046
Selectable Markers and Reporter Genes for Engineering the Chloroplast of Chlamydomonas reinhardtii
Lola Esland (2018)
The algal chloroplast as a synthetic biology platform for production of 1 therapeutic proteins 2 3
Y. M. Dyo (2018)
10.5772/INTECHOPEN.73401
Toward Future Engineering of the N-Glycosylation Pathways in Microalgae for Optimizing the Production of Biopharmaceuticals
Rodolphe Dumontier (2018)
10.3389/fpls.2019.00610
Characterization of a GDP-Fucose Transporter and a Fucosyltransferase Involved in the Fucosylation of Glycoproteins in the Diatom Phaeodactylum tricornutum
Peiqing Zhang (2019)
10.1016/j.tibtech.2019.12.023
Phaeodactylum tricornutum: A Diatom Cell Factory.
T. Butler (2020)
10.1007/s11274-018-2567-8
Manipulation of the microalgal chloroplast by genetic engineering for biotechnological utilization as a green biofactory
Yong Min Kwon (2018)
10.1016/j.biortech.2019.121890
Omics approaches for microalgal applications: Prospects and challenges.
A. Mishra (2019)
10.1007/978-3-319-66360-9_3
Chlamydomonas reinhardtii : Protein Glycosylation and Production of Biopharmaceuticals
Elodie Mathieu-Rivet (2017)
10.1186/s12934-017-0745-2
From hybridomas to a robust microalgal-based production platform: molecular design of a diatom secreting monoclonal antibodies directed against the Marburg virus nucleoprotein
F. Hempel (2017)
10.1186/s12934-019-1220-z
Using a marine microalga as a chassis for polyethylene terephthalate (PET) degradation
Daniel Moog (2019)
10.1099/mic.0.000599
The algal chloroplast as a synthetic biology platform for production of therapeutic proteins.
Y. M. Dyo (2018)
10.1080/21655979.2017.1377867
Green biologics: The algal chloroplast as a platform for making biopharmaceuticals
H. Taunt (2018)
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