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Label-Free Optical Biosensors For Monitoring Cellular Processes And Cytotoxic Agents At Interfaces Using Guided Modes And Advanced Phase-Contrast Imaging Techniques

I. Székács, R. Horváth, A. Székács
Published 2016 · Chemistry

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Novel optical biosensors in direct interaction with living cells open new avenues in the detection of given cell types, including pathogenic microorganisms, and in the measurement of cytotoxicity of given xenobiotics, such as biothreat agents, on suitably selected cell types or lines. Evanescent optical field based biosensors emerging for such applications are reviewed, covering surface plasmon and waveguide based formats, imaging setup, employing optical waveguides, and digital holographic microscopy. Commercial technologies, along with main pathogenic microorganisms (Bacillus anthracis, Escherichia coli, Listeria monocytogenes, Campylobacter jejuni, etc.) as analytes and cell lines (native tissue and tumor cells) as cytotoxicity effect subjects are highlighted.
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