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Light-Sheet Microscopy For Whole-Brain Imaging

M. Pawłowska, M. Stefaniuk, Diana Legutko, L. Kaczmarek
Published 2019 · Materials Science

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The brain is a complex system of interconnected and interacting structures. To fully understand its architecture, we need an imaging method that is capable of imaging the entire brain at sufficient resolution to capture single cells and projections and discriminate between cells with different functions. This has been made possible by the combination of light-sheet fluorescence microscopy with optical tissue clearing. In this chapter, we briefly describe the currently known methods of tissue clearing, discussing the advantages and disadvantages of different approaches. Next, we explain the principles of light-sheet fluorescence microscopy focusing on its application, the whole-brain imaging. We discuss the technical challenges specific to this approach, in particular those resulting from high refractive index of cleared tissue as well as from the large size of collected data. Finally, we list some of the recent papers which make use of light-sheet imaging of cleared tissue.
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