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Immunolocalization Of Abscisic Acid By Monoclonal Antibodies In Lavandula Stoechas L. Leaves

A. Pastor, N. Cortadellas, L. Alegre
Published 2004 · Biology

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A monoclonal antibody was used to localize abscisic acid in Lavandula stoechas L. plants treated with 1 μM abscisic acid. ABA localization was performed using EDC as the only fixative, which preserves antigenicity and improves the specificity of monoclonal antibodies. A relationship was observed between the effect of abscisic acid on chloroplast ultrastructure and ABA accumulation. Gold particles accumulated on swollen chloroplasts. Our findings provide cytochemical evidence that the chloroplast is a trapping compartment, and yield valuable information on the relation between chloroplast ultrastructure and ABA accumulation.
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