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An Improved Method For Monitoring Cell Death In Cell Suspension And Leaf Disc Assays Using Evans Blue

C. Jacyn Baker, N. Mock
Published 2004 · Biology

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Cell viability or cell death is an important variable to monitor in many studies of host/pathogen interactions. However for studies that focus on events within the first few hours of the interaction, many of the viability assays currently being used are either too laborious and time consuming or measure the cell's temporary metabolic state rather than irreversible cell death. Evans blue has proven over the years to be a dependable stain for microscopic determination of cell death. We have used this stain to develop a spectrophotometric procedure that allows rapid, reproducible quantification of the stain retained by dead cells. This spectrophotometric procedure was used to compare plant/bacteria interactions involving either soybean/Pseudomonas syringae pv. glycinea or tobacco/P. syringae pv. syringae. Relative increases in cell death during these interactions in suspension cell systems were measured by both the spectrophotometric and microscopic technique and found to be similar. The spectrophotometric procedure was also adapted for leaf disc assays.
This paper references
The hypersensitive reaction in tobacco : a reflection of changes in host cell permeability
RN Goodman (1968)
The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells.
J. Widholm (1972)
Early physiological responses associated with race-specific recognition in soybean leaf tissue and cell suspensions treated with Pseudomonas syringae pv. glycinea
E. W. Orlandi (1992)
Influence of daunorubicin on membrane permeability properties: detection by means of intracellular accumulation and efflux of fluorescein.
E. Prosperi (1985)
Involvement of plasma membrane calcium influx in bacterial induction of the k/h and hypersensitive responses in tobacco.
M. Atkinson (1990)
Concurrent loss in Tn5 mutants of Pseudomonas syringae pv. syringae of the ability to induce the hypersensitive response and host plasma membrane K+/H+ exchange in tobacco
C. J. Baker (1987)
The Use of Evan's Blue Stain to Test the Survival of Plant Cells after Exposure to High Salt and High Osmotic Pressure
J. Taylor (1980)
Freezing injury in potato leaves.
N. Sukumaran (1972)
Relationship between Vital Staining and Subculture Growth during the Senescence of Plant Tissue Cultures.
B. A. Smith (1982)
The Use of Non-permeating Pigments for Testing the Survival of Cells
D. Gaff (1971)
The use of neutral red and Evans blue for live-dead determinations of marine plankton (with comments on the use of rotenone for inhibition of grazing).
R. W. Crippen (1974)
Vital staining of root hairs in 12 warm-season perennial grasses.
M. J. Oprisko (1990)
The estimation of cell death in suspension cultures evoked by phytotoxic compounds: Differences among techniques
S. Zilkah (1978)
Early responses during plant-bacteria interactions in tobacco cell suspensions
C. J. Baker (1991)
Viability assays for preserved cells, tissues, and organs.
D. Pegg (1989)
A new fluorescent test for cell vitality using calcofluor white M2R.
J. Fischer (1985)
Plasma membrane alteration during bacteria-induced hypersensitive reaction in tobacco suspension cells as monitored by intracellular accumulation of fluorescein
L. Keppler (1988)
Inhibition of the hypersensitive response in tobacco by pectate lyase digests of cell wall and of polygalacturonic acid
C. J. Baker (1990)
Plant viability assay.
J. Palta (1978)
Criteria of life : some methods of measuring viability
J Parker (1953)
Recognition responses in pathogen/non-host and race/cultivar interactions involving soybean (Glycine max) and Pseudomonas syringae pathovars
C. Baker (1993)

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