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Computer Aided Fluorescence Imaging Of Photosynthetic Systems

J. Fenton, A. Crofts
Published 2004 · Computer Science, Medicine

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A fluorescence video imaging system utilizing relatively inexpensive commercial components is described. The instrument utilizes a black and white CCD video camera detector, a commercial video imaging board and a IBM-AT compatible computer. The color output of the imaging board greatly aids in the users ability to visually discriminate areas of interest in the video field. Software development that enables the user to capture kinetic traces in real time from the video images is also described. The system is used to monitor fluorescence from photosynthetic systems. The usefulness of the system in screening for photosynthetic mutants is also demonstrated. The cost of the system can be kept below $12,000.
This paper references
10.1016/0003-9861(85)90612-5
Characterization of light-harvesting mutants of Rhodopseudomonas sphaeroides. I. Measurement of the efficiency of energy transfer from light-harvesting complexes to the reaction center.
S. Meinhardt (1985)
10.1104/PP.84.3.748
Image analysis of chlorophyll fluorescence transients for diagnosing the photosynthetic system of attached leaves.
K. Omasa (1987)
10.1128/JB.154.2.748-755.1983
Isolation and characterization of enhanced fluorescence mutants of Rhodopseudomonas capsulata.
D. Youvan (1983)
Chlorophyll fluorescence: An intrinsic probe of photosynthesis. In: Govindjee (ed) Bioenergetics of Photosynthesis, pp 319-371
G Papageorgiou (1975)
10.1038/NBT0888-939
Applications of Imaging Spectroscopy in Molecular Biology: I. Screening Photosynthetic Bacteria
M. Yang (1988)
10.1007/BF00054144
Chlorophyll fluorescence yield changes as a tool in plant physiology I. The measuring system
U. Schreiber (2004)
10.1007/BF00041833
Chlorophyll fluorescence yield changes as a tool in plant physiology I. The measuring system
U. Schreiber (2004)
10.1128/MMBR.52.1.50-69.1988
Molecular genetics of photosynthetic membrane biosynthesis in Rhodobacter sphaeroides.
P. Kiley (1988)
10.1016/B978-0-12-294350-8.50011-8
6 – Chlorophyll Fluorescence: An Intrinsic Probe of Photosynthesis
G. Papageorgiou (1975)
10.1104/PP.90.4.1233
Topography of photosynthetic activity of leaves obtained from video images of chlorophyll fluorescence.
P. F. Daley (1989)
10.1007/BF00431600
A uniparental mutant of Chlamydomonas reinhardtii deficient in the chlorophyll-protein complex CP1
P. Bennoun (2004)
A uniparental
P Bennoun (1977)



This paper is referenced by
10.1074/jbc.M109.099069
CCS5, a Thioredoxin-like Protein Involved in the Assembly of Plastid c-Type Cytochromes*
Stéphane T. Gabilly (2010)
10.1023/A:1012461407557
A Microscope for Two-Dimensional Measurements of In Vivo Chlorophyll Fluorescence Kinetics Using Pulsed Measuring Radiation, Continuous Actinic Radiation, and Saturating Flashes
H. Küpper (2004)
Participation of nuclear genes in chloroplast gene expression
Alice Barkana (2019)
10.1366/0003702953965542
Imaging Fluorometer to Detect Pathological and Physiological Change in Plants
Li Ning (1995)
10.1023/A:1005769009069
Screening algal mutant colonies with altered thylakoid electrochemical gradient through fluorescence and delayed luminescence digital imaging
P. Bennoun (2004)
10.1016/S0076-6879(98)97025-2
[23]Application of spectroscopic techniques to the Study of Photosystem II Mutations Engineered in Synechocystis and Chlamydomonas
B. Diner (1998)
10.1366/0003702971938867
Recovery of Digital Information Stored in Living Plant Leaf Photosynthetic Apparatus as Fluorescence Signals
Li Ning (1997)
10.1023/A:1012485018944
Seeing Is Believing
L. N. Govindjee (2004)
10.1007/978-90-481-9268-7_5
Microscopic Measurements of the Chlorophyll a Fluorescence Kinetics
O. Komárek (2010)
10.1071/PP9950277
Quantitative mapping of leaf photosyn-thesis using chlorophyll fluorescence imaging
B. Genty (1994)
10.1002/PS.2780430406
Characterization of new photosynthesis-inhibiting imidazolidine derivatives. 1. Interpretation of post-emergence herbicidal performance from photosynthesis-inhibiting activity and systemicity
D. Yanase (1995)
10.1007/s11120-009-9415-5
Analytical approaches to photobiological hydrogen production in unicellular green algae
A. Hemschemeier (2009)
10.1093/JXB/ERL207
Multispectral fluorescence and reflectance imaging at the leaf level and its possible applications.
S. Lenk (2007)
10.1074/JBC.M208652200
Functional Analysis of a Divergent System II Protein, Ccs1, Involved in c-Type Cytochrome Biogenesis* 210
B. Dreyfuss (2003)
10.1007/978-94-009-0173-5_617
Screening for Mutants Deficient in State Transitions Using Time-Resolved Imaging Spectroscopy of Chlorophyll Fluorescence
J. F. Allen (1995)
10.1016/J.SCIENTA.2012.02.002
Applications of chlorophyll fluorescence imaging technique in horticultural research: A review
Elisa Gorbe (2012)
10.1093/JXB/ERH145
Imaging of chlorophyll a fluorescence: theoretical and practical aspects of an emerging technique for the monitoring of photosynthetic performance.
K. Oxborough (2004)
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