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Cytoplasmic Male Sterility (CMS) In Lolium Perenne L. 2. The Mitochondrial Genome Of A CMS Line Is Rearranged And Contains A Chimaeric Atp 9 Gene

A. Kiang, T. Kavanagh
Published 2004 · Biology, Medicine

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The most striking difference between the mtDNAs of the fertile L. perenne line LPSB21 and the male-sterile line CMS9B290, is the presence in the former and the absence in the latter of a 5.6-kb HindIII fragment. This difference between fertile and sterile lines was the starting point for a detailed molecular analysis of the mitochondrial genome in the region spanning the 5.6-kb HindIII fragment in fertile L. perenne and the corresponding region in CMS9B290. Restriction mapping and Southern-blot analyses indicated that rearrangement of the mitochondrial genome consistent with a deletion/insertion event had occurred in the sterile line. Nucleotide-sequence analysis of the rearranged region in CMS9B290 revealed the presence of (1) a novel chimaeric gene, orf-C9, comprising the first six codons of atp9 fused to a further 118 codons of an unknown sequence and (2) a truncated version of an open reading frame, orf-L, originally identified in LPSB21 mtDNA. Northern-blot analysis confirmed the absence of orf-L transcripts and the presence of orf-C9 transcripts in the mtRNA of CMS9B290.
This paper references
Wheat mkochondrial DNA: organization and sequence of atpA and atp9 genes
E Schulte (1989)
CYTOPLASMIC MALE-STERILITY IN SUNFLOWER IS CORRELATED WITH THE CO-TRANSCRIPTION OF A NEW OPEN READIN
R. Kohler (1991)
10.1002/j.1460-2075.1994.tb06230.x
Nuclear restoration of cytoplasmic male sterility in sunflower is associated with the tissue‐specific regulation of a novel mitochondrial gene.
F. Monéger (1994)
10.1007/BF00028860
Organ-specific reduction in the abundance of a mitochondrial protein accompanies fertility restoration in cytoplasmic male-sterile radish
S. Krishnasamy (2004)
10.1007/BF00222670
Cytoplasmic male sterility (CMS) in Lolium perenne L.: 1. Development of a diagnostic probe for the male-sterile cytoplasm
A. Kiang (2004)
10.1073/PNAS.82.4.1015
Nucleotide sequence of F(0)-ATPase proteolipid (subunit 9) gene of maize mitochondria.
R. Dewey (1985)
10.1515/9783111646831-021
GENETIC AND BIOCHEMICAL STUDIES OF THE NUCLEAR SUPPRESSOR NAM2 EXTRANEOUS ACTIVATION OF A LATENT PLEIOTROPIC MATURASE
G. Dujardin (1983)
10.1126/science.250.4983.942
The Texas Cytoplasm of Maize: Cytoplasmic Male Sterility and Disease Susceptibility
C. Levings (1990)
10.1093/NAR/16.11.5198
Speeding-up the sequencing of double-stranded DNA.
G. Murphy (1988)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
10.1105/tpc.1.11.1121
Identification of a mitochondrial protein associated with cytoplasmic male sterility in petunia.
H. Nivison (1989)
10.1002/j.1460-2075.1987.tb02398.x
A mitochondrial gene is lost via homologous recombination during reversion of CMS T maize to fertility
W. Rottmann (1987)
10.1007/BF00273922
Transcription of the Petunia mitochondrial CMS-associated Pcf locus in male sterile and fertility-restored lines
K. D. Pruitt (2004)
10.1007/BF00284202
Ogura cytoplasmic male-sterility (CMS)-associated orf138 is translated into a mitochondrial membrane polypeptide in male-sterile Brassica cybrids
M. Grelon (2004)
10.1002/j.1460-2075.1993.tb05787.x
Processing followed by complete editing of an altered mitochondrial atp6 RNA restores fertility of cytoplasmic male sterile rice.
M. Iwabuchi (1993)
10.1007/BF00039566
A chimeric gene (orf256) is expressed as protein only in cytoplasmic male-sterile lines of wheat
J. Song (2004)
10.1093/NAR/17.18.7531
Wheat mitochondrial DNA: organization and sequences of the atpA and atp9 genes.
E. Schulte (1989)
10.1007/BF00224279
Molecular aspects of cytoplasmic male sterility in perennial ryegrass (Lolium perenne L.): mtDNA and RNA differences between plants with male-sterile and fertile cytoplasm and restriction mapping of their atp6 and coxI homologous regions
G. J. Rouwendal (2004)
10.1007/BF00032737
Cytoplasmic male sterility in ryegrasses (Lolium SPP.) detected after intergeneric hybridization
F. Wit (2004)
10.1007/BF00273925
Cytoplasmic male sterility in sunflower is correlated with the co-transcription of a new open reading frame with the atpA gene
Rainer Hans Köhler (2004)
Cytop - lasmic male sterility ( CMS ) in Lolium perenne L . 1 . Development of a diagnostic probe for the malesterile cytoplasm
Kiang A-S (1993)
10.1007/BF00259445
A chimeric gene containing the 5′ portion of atp6 is associated with cytoplasmic male-sterility of rice
K. Kadowaki (2004)
10.1111/J.1365-313X.1991.00185.X
Mitochondrial genome organization and expression associated with cytoplasmic male sterility in sunflower (Helianthus annuus).
H. K. Laver (1991)
10.1007/BF00351786
Mitochondrial DNA variability detected in a single wheat regenerant involves a rare recombination event across a short repeat
C. Hartmann (2004)
10.1073/PNAS.84.9.2858
Mutation to male fertility and toxin insensitivity in Texas (T)-cytoplasm maize is associated with a frameshift in a mitochondrial open reading frame.
R. Wise (1987)
10.1007/BF00326239
Sequence analysis of wheat mitochondrial transcripts capped in vitro: definitive identification of transcription initiation sites
P. Covello (2004)
10.1146/ANNUREV.GE.25.120191.002333
Plant mitochondrial mutations and male sterility.
M. Hanson (1991)
10.1093/NAR/18.2.370
Sequence of the F0-atpase proteolipid (atp9) gene from rice mitochondria.
E. Kaleikau (1990)
10.1093/NAR/14.20.7995
Sequence and transcription analysis of the Petunia mitochondrial gene for the ATP synthase proteolipid subunit.
E. Young (1986)
10.1007/BF00222893
Paternal inheritance of mitochondria and chloroplasts in Festuca pratensis-Lolium perenne intergeneric hybrids
A. Kiang (2004)
10.1016/0092-8674(87)90660-X
A fused mitochondrial gene associated with cytoplasmic male sterility is developmentally regulated
Ellora G. Young (1987)
10.1007/BF00254817
Induction of cytoplasmic male-sterility into ryegrass (Lolium perenne)
V. Connolly (2004)



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