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Evidence From Inhibitor Studies That The Endophyte Synthesises Nitrogenase In The Root Nodules Of Alnus Glutinosa L. Gaertn.

R. Skeffington, W. Stewart
Published 2004 · Biology, Medicine

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SummaryNitrogenase activity (acetylene reduction assay) in the nodulated non-leguminous angiosperm Alnus glutinosa is inhibited within minutes when plants are exposed to a gas phase containing 90% oxygen On returning the plants to air, nitrogenase activity recovers within a few hours, both in the presence of cycloheximide, which inhibits protein synthesis on 80 S (eukaryotic) ribosomes, and in the absence of inhibitor. When chloramphenicol, which inhibits protein synthesis on 70 S (prokaryotic) ribosomes, is added instead of cycloheximide, recovery from oxygen inhibition does not occur, or occurs only slowly. The effects of chloramphenicol are specific to the D-threo-isomer which indicates a direct inhibition of protein synthesis. Erythromycin has a similar effect to chloramphenicol. Protein biosynthesis in non-nodulated roots is inhibited by cycloheximide but not by chloramphenicol. The results are interpreted as evidence that the nitrogenase within Alnus glutinosa root nodules is synthesised by the microbial symbiont.
This paper references
10.1093/OXFORDJOURNALS.AOB.A083291
The Fixation of Nitrogen associated with the Root Nodules of Myrica gale L., with Special Reference to its pH Relation and Ecological Significance
G. Bond (1951)
10.1016/s0021-9258(19)52451-6
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)
Protein measurement with the Folin reagent
O. H. Lowry (1951)
10.1038/200596B0
Chloramphenicol and Uptake of Salt in Plants
R. Ellis (1963)
10.1021/AC60226A017
MEASUREMENT OF LOW ENERGY BETA-EMITTERS IN AQUEOUS SOLUTION BY LIQUID SCINTILLATION COUNTING OF EMULSIONS.
M. Patterson (1965)
10.1016/S0076-6879(67)12104-6
[91] Use of filters to separate radioactivity in RNA, DNA, and protein
D. Kennell (1967)
10.1104/PP.42.9.1297
Activation of protein synthesis by microsomes from aging beet disks.
R. Ellis (1967)
10.1073/PNAS.58.5.2071
In situ studies on N2 fixation using the acetylene reduction technique.
W. Stewart (1967)
10.1038/222791A0
Does Cycloheximide inhibit Protein Synthesis specifically in Plant Tissues?
I. Macdonald (1969)
10.1128/MMBR.36.2.231-260.1972
Dinitrogen (N 2 ) fixation (with a biochemical emphasis).
H. Dalton (1972)
10.1086/407182
A Tropical Rain Forest. A Study of Irradiation and Ecology at El Verde, Puerto Rico.Howard T. Odum , Robert F. Pigeon
B. Holt (1972)
10.1111/j.1469-185X.1972.tb00971.x
BIOCHEMISTRY OF PROTEIN SYNTHESIS IN PLANTS
D. Boulter (1972)
10.1111/J.1399-3054.1973.TB01184.X
Studies on the Genetic Control of a Nitrogenase Component in Leguminous Root Nodules
D. Phillips (1973)
10.1097/00010694-197605000-00012
The biology of nitrogen fixation
A. Quispel (1974)
10.1016/S0006-291X(74)80357-8
Genetic transfer of nitrogen fixation from Rhizobium trifolii to Klebsiella aerogenes.
L. K. Dunican (1974)
Root nodule symbiosis with actinomycete-like organisms
G. Bond (1974)
10.1038/256409A0
Acetylene reduction by Rhizobium in pure culture
J. McComb (1975)
10.1038/256407A0
Nitrogenase activity in rhizobia in absence of plant host
W. Kurz (1975)
10.1038/256406A0
Nitrogen fixation by Rhizobium cultured on a defined medium
J. Pagan (1975)
Reductants for nitrogenase and relationships to cellular electron transport
H. J. Evans (1975)



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