Online citations, reference lists, and bibliographies.
← Back to Search

Extraction, Distribution And Biodegradation Of Bacterial Lipopolysaccharides In Estuarine Sediments

J. N. Saddler, A. Wardlaw
Published 2004 · Biology, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
Lipopolysaccharides (LPS), added as whole bacteria to estuarine sediments, were extracted efficiently by both trichloroacetic acid (TCA) and phenol-water (PW). Amounts of recovered LPS were measured indirectly by analyses for ketodeoxyoctonate (KDO), β-hydroxymyristic acid, immunodominant sugars and anticomplementary (AC) activity towards human complement. TCA was judged to be better than PW for routine extraction of sediments because, although it yielded 10–20% less LPS, it avoided contamination with non-LPS, high-molecular weight material with high AC activity. In sediment samples taken as cores from estuarine beaches, the concentration of endogenous LPS diminished rapidly with depth below the topmost 1 cm. KDO disappeared more rapidly with depth than AC activity. When known LPS was incubated with estuarine beach mud at 20–22°C for 3 weeks there was extensive biodegradation of both the lipid and polysaccharide components, the latter more rapidly. LPS-degrading bacteria were isolated.
This paper references
10.1111/J.1432-1033.1976.TB10354.X
The role of the physical state of lipopolysaccharides in the interaction with complement. High molecular weight as prerequisite for the expression of anti-complementary activity.
C. Galanos (1976)
Structure, endotoxicity, immunogenicity of the lipid A component of bacterial lipopolysaccharides, p
O. (1975)
10.2307/3225004
Handbook of micromethods for the biological sciences
G. Keleti (1973)
Activation of the alternative complement pathway by pneumococcal cell wall teichoic acid.
J. Winkelstein (1978)
10.1016/0304-3770(75)90030-3
Aquatic microbiology: G. Rheinheimer. John Wiley & Sons, Ltd., New York, N.Y., 1974.
T. Fenchel (1975)
Technique p 0 ~ ar la prhparation des polyosides microbiens specifiques . - - Compt
D. C. ELLWOOD (1933)
Structure , endotoxicity , immunogenicity of the lipid A component of bacterial lipopolysaccharides , p . 307 314 . In
D. Schlessinger (1975)
drinking water and renovated waste water.--Appl
M. KATES (1972)
Activation of the alternative complement pathway by water-insoluble glucans of streptococcus mutans: the relation between their chemical structures and activating potencies.
S. Inal (1976)
10.1007/BF01944803
Biodegradation of microbial lipopolysaccharides
J. P. Voets (2005)
Rapid detection of bacterial endotoxins
J M.LEE (1976)
A sensitive colorimetric method for the estimation of 2-deoxy sugars with the use of the malonaldehyde-thiobarbituric acid reaction.
V. Waravdekar (1959)
10.1515/znb-1952-9-1010
Über bakterielle Reizstoffe I. Mitt.: Reindarstellung eines Polysaccharid-Pyrogens aus Bacterium coli
O. Westphal (1952)
The role of the physical state of lipopolysaccharide in the interaction with complement
N. KAZUYOSHI (1976)
10.1007/BF01922973
Superiority of human complement for assaying bacterial lipopolysaccharides by their anticomplementary activity
J. N. Saddler (2005)
The distribution of 2-keto-3-deoxyoctonic acid in bacterial walls.--J. Gen
D C. (1970)
10.1128/AEM.35.2.376-382.1978
Rapid determination of bacteriological water quality by using limulus lysate.
T. M. Evans (1978)
10.1128/AEM.32.3.347-351.1976
Rapid detection of bacterial endotoxins in drinking water and renovated wastewater.
J. Jorgensen (1976)
Simplified method for the prepapation of silica media
J. P. VOETS (1974)
10.1099/00221287-60-3-373
The distribution of 2-keto-3-deoxy-octonic acid in bacterial walls.
D. C. Ellwood (1970)
Ober bakterielle Reizstoffe 1
F. (1952)
A list of marine bacteria including descriptions of sixty new species.--Bull
C. E. ZOBELL (1944)
10.1128/MMBR.20.3.166-188.1956
Bacterial hemagglutination and hemolysis.
E. Neter (1956)
10.1111/J.1432-1033.1971.TB01298.X
Interaction of lipopolysaccharides and lipid A with complement.
C. Galanos (1971)
The role of the physical state of lipopolysaccharide in the interaction with complement.--Eur
O. LiJBEmTZ (1976)
1978.Activation of the alternative complement pathway by Pneumococcal cell wall teichoic acid.--J
J. A. WINKELSTEIN (1978)
10.1128/AEM.28.5.887-888.1974
Simplified method for the preparation of silica gel media.
R. Thatcher (1974)



This paper is referenced by
10.1016/0038-0717(85)90138-5
Extraction of bacterial lipopolysaccharides from soil samples and their determination using the Limulus amebocyte lysate reaction
S. R. Ford (1985)
10.1002/TOX.2540010305
Environmental effects testing with quantitative microbial analysis: Chemical signatures correlated with in situ biofilm analysis by FT/IR
D. White (1986)
10.1007/978-3-642-60966-4_5
Community Structure of Soil Microorganisms
L. Zelles (1996)
10.1007/BFB0008733
Evaluation of biomass.
A. Singh (1994)
10.1007/978-94-011-4928-0_2
Molecular Approaches to Microbial Biomass Estimation in the Sea
D. Karl (1998)
10.3929/ethz-a-004173317
Automated image analysis as a tool to study abundance of fungi and bacteria during leaf litter decomposition
Frank Schönholzer (2000)
10.1007/BF00007373
Biochemical markers for measurement of predation effects on the biomass, community structure, nutritional status, and metabolic activity of microbial biofilms
D. White (2004)
The biodegradation of bacterial lipopolysaccharides by marine invertebrates and marine bacteria
T. Jamieson (1986)
10.1007/BF00422014
The budding bacteria, Pirellula and Planctomyces, with atypical 16S rRNA and absence of peptidoglycan, show eubacterial phospholipids and uniquely high proportions of long chain beta-hydroxy fatty acids in the lipopolysaccharide lipid A
B. Kerger (2004)
10.1016/0146-6380(84)90123-2
Carbohydrates in recent marine sediments—II. Occurrence and fate of carbohydrates in a recent stromatolitic deposit: Solar Lake, Sinai
J. Klok (1984)
MOLECULAR APPROACHES TO MICROBIAL BIOMASS ESTIMATION IN THE SEA 2
M. (2010)
10.1007/978-3-642-71215-9_3
Forms of Biomass
S. M. Stronach (1986)
10.1146/ANNUREV.MI.44.100190.003051
Determination of bacterial growth and activity at solid-liquid interfaces.
G. Geesey (1990)
Humoral Immune Response of Inactivated Bivalent Leptospira Vaccine among Dogs in Tiruchirappalli
K. Natarajaseenivasan (2012)
10.1016/B978-012513840-6/50024-0
9 – Community structure
K. Alef (1995)
10.1007/BF02097406
Equivalence of microbial biomass measures based on membrane lipid and cell wall components, adenosine triphosphate, and direct counts in subsurface aquifer sediments
D. Balkwill (2005)
10.1007/978-1-4899-3564-9_16
Use of Lipid Biomarkers in Environmental Samples
A. Tunlid (1990)
10.1016/S0016-7037(96)00315-8
Rates and mechanisms of fatty acid degradation in oxic and anoxic coastal marine sediments of Long Island Sound, New York, USA
Ming-Yi Sun (1997)
10.1016/S0070-2161(08)60309-3
Lipopolysaccharides of Gram-Negative Bacteria
O. Lüderitz (1982)
10.1128/AEM.44.5.1170-1177.1982
Sensitive assay, based on hydroxy fatty acids from lipopolysaccharide lipid A, for Gram-negative bacteria in sediments.
J. Parker (1982)
10.1016/J.ORGGEOCHEM.2006.04.007
Biomarker analysis of microbial diversity in sediments of a saline groundwater seep of Salt Basin, Nebraska
J. Fang (2006)
10.1016/0146-6380(88)90116-7
The mode of occurrence of lipids in a Namibian Shelf diatomaceous ooze with emphasis on the β-hydroxy fatty acids
J. Klok (1988)
10.1016/0305-0491(89)90174-0
Degradation of bacterial lipopolysaccharides by digestive-gland extracts of marine bivalve molluscs
T. Jamieson (1989)
Cell biology of anammox Planctomycetes and methanotrophic Verrucomicrobia
M.C.F. van Teeseling (2016)
10.1038/373293C0
Organic carbon in sediments
W. A. Hartgers (1995)
10.1007/s003740050533
Fatty acid patterns of phospholipids and lipopolysaccharides in the characterisation of microbial communities in soil: a review
L. Zelles (1999)
10.4236/WJV.2012.22011
Humoral Immune Response of Inactivated Bivalent Leptospira Vaccine among Dogs in Tiruchirappalli, Tamilnadu, India
K. Natarajaseenivasan (2012)
Semantic Scholar Logo Some data provided by SemanticScholar