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Replication Of Mini-F Plasmid In Vitro Promoted By Purified E Protein

K. Muraiso, T. Tokino, T. Murotsu, K. Matsubara
Published 2004 · Biology, Medicine

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SummaryAn in vitro system for replication of mini-F plasmid DNA was constructed. This system consists of an ammonium sulfate fraction II (Fuller et al. 1981) from Escherichia coli extract, exogeneously added purified E protein encoded by mini-F plasmid, and mini-F DNA in a closed circular form. Experiments with this system showed that the 217 bp DNA region which contains the A+T rich cluster and the four 19 bp direct repeats responsible for incB incompatibility is essential for mini-F DNA replication.
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