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Urease Activity And Its Michaelis Constant For Soil Systems

V. Beri, K. P. Goswami, S. Brar
Published 2005 · Chemistry

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SummaryUrea hydrolysis was measured in two separate sets of experiments. (1) Nine soil (0–15 cm) samples were treated with 200 μg of urea-N g−1 dry soil and incubated (at 37°C) at 50 per cent of the water holding capacity. Samples were periodically analysed for the remaining urea-N. The urease activity (time in hoursrequired to hydrolyse half the applied urea-N) was determined to be 5.8 to 15.2 hours in the various soils, which appeared to associate principally with the organic carbon content of the soils (r=−0.80**). (2) Three soils were treated with 25 to 2000 μg urea-N g−1 dry soil amounting to 0.9 to 72.0 mM urea in 1∶1 soil: solution. The system was buffered at pH 7.2 and agitated for 5h when the remaining urea-N was determined. The values of Km and Vmax were computed by two methods (i) from the integrated form of the Michaelis-Menten equation based on the results of the first study, and (ii) from the Michaelis-Menton equation based on urea hydrolysis in the second study. The integrated method appeared to be more suitable for enzyme kinetic studies in soil systems where the Km and Vmax values bore close relationship (r=−0.88**).
This paper references
10.2136/SSSAJ1952.03615995001600030014X
Field and Laboratory Studies of Nitrite Accumulation in Soils1, 2
H. D. Chapman (1952)
10.1007/BF01349175
The urease activity in profiles of five great soil groups from northern New South Wales
Margaret G. Myers (2005)
10.1016/0038-0717(72)90048-X
Extraction of an urease-active organo-complex from soil.
R. Burns (1972)
10.2136/SSSAJ1973.03615995003700020020X
Effects of Some Urease Inhibitors on Urea Hydrolysis in Soils 1
J. M. Bremner (1973)
10.2136/SSSAJ1970.03615995003400060015X
Extraction and colorimetric determination of urea in soils.
L. A. Douglas (1970)
10.1007/BF00779021
Factors affecting urea hydrolysis in several alberta soils
W. D. Gould (2004)
10.2136/SSSAJ1970.03615995003400040019X
Urea conversion to ammonia in waterlogged soils.
R. D. Delaune (1970)
10.1007/BF01373391
A survey of urease activity in soils of Northern New South Wales
J. McGarity (2005)
10.2136/SSSAJ1966.03615995003000020018X
Ureolysis, Immobilization, and Nitrification in Black Spruce (Picea mariana Mill.) Humus1, 2
M. R. Roberge (1966)
10.2136/SSSAJ1970.03615995003400010021X
Michaelis constant of soil urease.
K. Paulson (1970)
10.2136/SSSAJ1972.03615995003600020030X
Concerning the Location and Persistence of Soil Urease
R. Burns (1972)
10.1007/BF00016966
Some measures of reducing leaching loss of nitrates beyond potential rooting zone
B. Singh (2004)
10.2136/SSSAJ1963.03615995002700010019X
Urea Hydrolysis and Transformation in Some Illinois Soils1
D. Simpson (1963)
10.2136/SSSAJ1941.036159950005000C0040X
Catalytic Activity Causing the Hydrolysis of Urea in Soils as Influenced by Several Agronomic Factors 1
J. P. Conrad (1941)
10.2136/SSSAJ1973.03615995003700050024X
Michaelis Constants of Urease in Soils and Soil Fractions 1
M. Tabatabai (1973)
10.1007/BF00015890
Some measures of reducing leaching loss of nitrates beyond potential rooting zone
B. Singh (2004)
10.1016/0038-0717(71)90040-X
A rapid method of evaluating different compounds as inhibitors of urease activity in soils
L. Douglas (1971)



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10.1007/BF00260846
Short- and long-term effects of heavy metals on urease activity in soils
P. Doelman (2004)
10.1007/BF02374889
Dehydrogenase and urease activities of maize (Zea mays L.) field soils
M. Dkhar (2006)
TAK 1 Determination of the Effects of Hazelnut Husk and Tea Waste Treatments on Urease Enzyme Activity and Its Kinetics in Soil R ›
D. Kizilkaya (2008)
MICROBIAL COMMUNITIES AND ENZYME KINETICS USED AS INDEX OF RECLAMATION IN A CHRONOSEQUENCE COAL MINE OVERBURDEN SPOIL
J. Patel (2013)
Impact of the Herbicide Oxadiazon on Microbial Activity and Nitrogen Dynamics in Soil Environment
M. Rahman (2004)
10.1007/BF00257763
Microbial populations, enzyme activities and nitrogen-phosphorus-potassium enrichment in earthworm casts and in the surrounding soil of a pineapple plantation
S. Tiwari (2004)
Kinetic and thermodynamic properties of hydrolases in Northeastern China soils affected by temperature
Y. Zhang (2010)
10.1016/j.ecoenv.2017.01.036
Phytoextraction of toxic trace elements by Sorghum bicolor inoculated with Streptomyces pactum (Act12) in contaminated soils.
Amjad Ali (2017)
10.31018/JANS.V9I4.1551
Herbicidal effect on the bio-indicators of soil health- A review
S. K. Raj (2017)
MINERAL NITROGEN CONTENTS IN CANE-CROPPED VERTISOLS OF SUDAN AND GUADELOUPE AS INFLUENCED BY UREA APPLICATION MANAGEMENT
M. Brouwers (2008)
Determination of the Effects of Hazelnut Husk and Tea Waste Treatments on Urease Enzyme Activity and Its Kinetics in Soil
R. Kızılkaya (2008)
10.1016/j.tsf.2020.138043
Effect of interfering agents for urease immobilized in Langmuir-Blodgett films of controlled molecular architecture✰
R. Rodrigues (2020)
10.4067/S0718-27912009000200003
CATALYTIC POTENTIAL OF SOIL HYDROLASES IN NORTHEAST CHINA UNDER DIFFERENT SOIL MOISTURE CONDITIONS
Y. Zhang (2009)
Influence of Long Term Nitrogen and Potassium Fertilization on the Biochemistry of Tea Soil
K. Thenmozhi (2012)
10.1007/s42729-019-00089-w
The Burning of Sugarcane Plantation in the Tropics Modifies the Microbial and Enzymatic Processes in Soil and Rhizosphere
A. Trujillo-Narcía (2019)
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