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Aptamers: Molecular Tools For Analytical Applications

T. Mairal, Veli Cengiz Özalp, Pablo Lozano Sánchez, M. Mir, I. Katakis, C. O'Sullivan
Published 2008 · Medicine, Chemistry

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Aptamers are artificial nucleic acid ligands, specifically generated against certain targets, such as amino acids, drugs, proteins or other molecules. In nature they exist as a nucleic acid based genetic regulatory element called a riboswitch. For generation of artificial ligands, they are isolated from combinatorial libraries of synthetic nucleic acid by exponential enrichment, via an in vitro iterative process of adsorption, recovery and reamplification known as systematic evolution of ligands by exponential enrichment (SELEX). Thanks to their unique characteristics and chemical structure, aptamers offer themselves as ideal candidates for use in analytical devices and techniques. Recent progress in the aptamer selection and incorporation of aptamers into molecular beacon structures will ensure the application of aptamers for functional and quantitative proteomics and high-throughput screening for drug discovery, as well as in various analytical applications. The properties of aptamers as well as recent developments in improved, time-efficient methods for their selection and stabilization are outlined. The use of these powerful molecular tools for analysis and the advantages they offer over existing affinity biocomponents are discussed. Finally the evolving use of aptamers in specific analytical applications such as chromatography, ELISA-type assays, biosensors and affinity PCR as well as current avenues of research and future perspectives conclude this review.
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Rapid, Affordable, and Point-of-Care Water Monitoring Via a Microfluidic DNA Sensor and a Mobile Interface for Global Health
Unyoung Kim (2013)
10.1117/1.3465594
Direct detection of aptamer-thrombin binding via surface-enhanced Raman spectroscopy.
Cynthia V. Pagba (2010)
10.1504/IJENVH.2011.041327
Aptamer-based and DNAzyme-based biosensors for environmental monitoring
M. Fusco (2011)
10.3390/cancers10010009
Current Advances in Aptamers for Cancer Diagnosis and Therapy
Shin-ichiro Hori (2018)
10.1021/ac500444m
G-quadruplex on oligo affinity support (G4-OAS): an easy affinity chromatography-based assay for the screening of G-quadruplex ligands.
D. Musumeci (2014)
10.1016/j.biomaterials.2013.12.060
Bacterial imaging with photostable upconversion fluorescent nanoparticles.
L. Ong (2014)
10.1016/j.chroma.2014.09.040
Optimization of a microfluidic electrophoretic immunoassay using a Peltier cooler.
Nikita Mukhitov (2014)
10.1016/J.SNB.2011.03.008
Electrochemical DNA aptamer-based biosensor for OTA detection, using superparamagnetic nanoparticles
L. Barthelmebs (2011)
10.21236/ada538326
Method for Single-Cell Mass and Electrophoretic Mobility Measurement
P. Dextras (2010)
Development of an Enzyme-linked Aptamer-based Assay for the Detection of Tetracycline
Z. Xua (2014)
10.1002/elps.200800058
Immunoaffinity capillary electrophoresis as a powerful strategy for the quantification of low‐abundance biomarkers, drugs, and metabolites in biological matrices
N. Guzman (2008)
10.1016/j.addr.2011.10.002
Aptamer-conjugated nanomaterials and their applications.
L. Yang (2011)
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