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Calorimetric And EPR Studies Of The Thermotropic Phase Behavior Of Phospholipid Membranes
D. Pentak, W. W. Sułkowski, A. Sułkowska
Published 2008 · Chemistry
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Transmission electron micrographs (TEM) showed that liposome vesicles prepared from DL-α-phosphatidylcholine dimyristoyl (1,2-ditetradecanoyl-rac-glycerol-3-phosphocholine) (DMPC) by the modified reverse-phase evaporation method (mREV) were spherical in shape and in majority of them were less than 100 nm in diameter. Differential scanning calorimetry (DSC) method was used to determine the influence of cholesterol content and pH of Tris-HCl buffer used for the preparation of liposomes on the temperature of phase transition TC of phospholipids which form the investigated liposome vesicles. The use of DSC method made it possible to determine not only the temperature of the main phase transition of phospholipids but also the temperature of the phospholipid phase transition from the tilted gel phase(Lβ′) to the ripple gel phase(Pβ′). The results were compared with those obtained with EPR study. EPR study was carried out in the temperature range from 284 to 310 K i.e. below and above the phase transition temperature TC of DMPC. On the basis of EPR spectra of spin marker 2,2,6,6-tetramethyl-1-piperidinyloxy (TEMPO) incorporated into the liposome, the values of parameters f were determined. Hence TEMPO can be used to observe the change in partition between aqueous and fluid lipid regions. The change in the relative values of f determined for DMPC as a function of temperature shows that this phospholipid undergoes a transition from a ‘gel phase’ to a lamellar smectic liquid crystalline phase in the presence of excess water. The EPR study of TEMPO allowed us to determine the transition temperature TC. The results were compared with those obtained with DSC method.
This paper references
Polyhistidine mediates an acid-dependent fusion of negatively charged liposomes.
C. Y. Wang (1984)
The role of liposomes in analytical processes
A. Gómez-Hens (2005)
Effects of pH and cholesterol on DMPA membranes: a solid state 2H- and 31P-NMR study.
T. Pott (1995)
The effect of bilayer order and fluidity on detergent‐induced liposome fusion
R. Sáez (1985)
Lipid bilayers: thermodynamics, structure, fluctuations, and interactions.
S. Tristram-Nagle (2004)
Control of lipid membrane stability by cholesterol content.
S. Raffy (1999)
Molecular order and dynamics of phosphatidylcholine bilayer membranes in the presence of cholesterol, ergosterol and lanosterol: a comparative study using 2H-, 13C- and 31P-NMR spectroscopy.
J. Urbina (1995)
Structure of dipalmitoylphosphatidylcholine/cholesterol bilayer at low and high cholesterol concentrations: molecular dynamics simulation.
A. Smondyrev (1999)
Electron spin resonance study of phospholipid membranes employing a comprehensive line-shape model.
A. Lange (1985)
The Rijndael algorithm
T. Jamil (2004)
Small-volume extrusion apparatus for preparation of large, unilamellar vesicles.
R. Macdonald (1991)
Spin-labeling study of membranes in wheat embryo axes. 1. Partitioning of doxyl stearates into the lipid domains.
E. A. Vishnyakova (2000)
Procedure for preparation of liposomes with large internal aqueous space and high capture by reverse-phase evaporation.
F. Szoka (1978)
Synthesis and vesicle formation from dimeric pseudoglyceryl lipids with (CH2)(m) spacers: pronounced m-value dependence of thermal properties, vesicle fusion, and cholesterol complexation
S. Bhattacharya (1999)
Cholesterol effects on the phosphatidylcholine bilayer polar region: a molecular simulation study.
M. Pasenkiewicz-Gierula (2000)
pH-dependent fusion of liposomes using titratable polycations.
P. S. Uster (1985)
Temperature scanning X-ray diffraction at phase transitions of biologically related lipid assemblies
I. Hatta (2005)
Effect of anti-inflammatory drugs on splenocyte membrane fluidity.
H. Ferreira (2005)
The effect of fructan on membrane lipid organization and dynamics in the dry state.
I. Vereyken (2003)
Effects of divalent cations and polyethylene glycol on the membrane fluidity of protoplast.
W. Boss (1980)
The influence of temperature, cholesterol content and pH on liposome stability
W. Sułkowski (2005)
Influence of cholesterol on liposome fluidity by EPR. Relationship with percutaneous absorption.
L. Coderch (2000)
Modulation of membrane fusion by membrane fluidity: temperature dependence of divalent cation induced fusion of phosphatidylserine vesicles.
J. Wilschut (1985)
Phase Separations in Phospholipid Membranes
S. H. Wu (1975)
Interactions of cationic surfactantswith DPPC
M. Kozak (2007)
Selective Protein-Lipid Interactions at Membrane Surfaces: A Deuterium and Phosphorus Nuclear Magnetic Resonance Study of the Association of Myelin Basic Protein with the Bilayer Head Groups of Dimyristoylphosphatidylcholine and Dimyristoylphosphatidylglycerol
F. Sixl (1984)
Evidence for phase boundary lipid. Permeability of Tempo-choline into dimyristoylphosphatidylcholine vesicles at the phase transition.
D. Marsh (1976)
Novel applications of liposomes.
D. Lasic (1998)
Phase behaviour of polymer-grafted DPPC membranes for drug delivery systems design
M. Pappalardo (2005)
Effect of temperature on liposome structures studied using EPR spectroscopy
W. W. Sułkowski (2005)
Fluorescence study of binding of adenosine derivatives to phospholipid membranes — effect of serum albumin
A. Sułkowska (2001)
1-[2-Hydroxy-3-octadecan-1'-oate]propyl-2'',2'',5'',5''-tetramethyl pyrolidine-N-oxyl-3''-carboxylate as a potential spin probe for membrane structure studies.
R. Katoch (1999)
Liposomes: from biophysics to the design of peptide vaccines.
F. Frézard (1999)
Fluorescence depolarization studies of phase transitions and fluidity in phospholipid bilayers. 2 Two-component phosphatidylcholine liposomes.
B. Lentz (1976)
The influence of temperature and pH on the structure of liposomes formed from DMPC
W. W. Sułkowski (2006)
Membrane properties of archaeal macrocyclic diether phospholipids.
O. Dannenmuller (2000)
Interaction of the neuropeptide met-enkephalin with zwitterionic and negatively charged bicelles as viewed by 31P and 2H solid-state NMR.
I. Marcotte (2003)
Distortion of the lamellar arrangement of phospholipids by deep rough mutant lipopolysaccharide from Salmonella minnesota
E. Urbán (2005)
Effect of tetracaine on model and erythrocyte membranes by DSC and EPR
F. Könczöl (2005)
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M. R. Sovizi (2010)
Characterization of cationic liposomes. Influence of the bilayer composition on the kinetics of the liposome breakdown.
G. Angelini (2011)
Effect of amyloid beta peptides Aβ1–28 and Aβ25–40 on model lipid membranes
M. Ionov (2010)
Cholesterol levels and activity of membrane bound proteins: characterization by thermal and electrochemical methods
Noufissa Zanati (2009)
The acetylcholinesterase (AChE) inhibitor and anti-Alzheimer drug donepezil interacts with human erythrocytes.
Pablo A. Zambrano (2019)
Small-Scale Sample Handling for Studies of Liquid Crystals and Lipid-Based Soft Matter
G. Ohlsson (2012)
Interactions of the antimalarial amodiaquine with lipid model membranes.
R. Barroso (2015)
Alternative methods of determining phase transition temperatures of phospholipids that constitute liposomes on the example of DPPC and DMPC
D. Pentak (2014)
Large effect of membrane tension on the fluid–solid phase transitions of two-component phosphatidylcholine vesicles
D. Chen (2013)
The effect of neuroleptic drugs on DPPC/sphingomyelin/cholesterol membranes.
R. Pérez-Isidoro (2020)
Design of liposomes containing photopolymerizable phospholipids for triggered release of contents
Amichai Yavlovich (2009)
Thermal behavior of drugs
M. R. Sovizi (2010)
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D. Chen (2014)
Three dimensional (temperature-tension-composition) phase map of mixed DOPC-DPPC vesicles: Two solid phases and a fluid phase coexist on three intersecting planes.
D. Chen (2014)
Smart photothermal-triggered bilayer phase transition in AuNPs-liposomes to release drug.
X. An (2013)