Online citations, reference lists, and bibliographies.
← Back to Search

Internalisation Of Engineered Nanoparticles Into Mammalian Cells In Vitro: Influence Of Cell Type And Particle Properties

W. Busch, S. Bastian, Ulrike Trahorsch, M. Iwe, Dana Kühnel, T. Meißner, A. Springer, M. Gelinsky, V. Richter, C. Ikonomidou, A. Potthoff, I. Lehmann, K. Schirmer
Published 2011 · Materials Science

Save to my Library
Download PDF
Analyze on Scholarcy
Share
Cellular internalisation of industrial engineered nanoparticles is undesired and a reason for concern. Here we investigated and compared the ability of seven different mammalian cell cultures in vitro to incorporate six kinds of engineered nanoparticles, focussing on the role of cell type and particle properties in particle uptake. Uptake was examined using light and electron microscopy coupled with energy dispersive X-ray spectroscopy (EDX) for particle element identification. Flow cytometry was applied for semi-quantitative analyses of particle uptake and for exploring the influence on uptake by the phagocytosis inhibitor Cytochalasin D (CytoD). All particles studied were found to enter each kind of cultured cells. Yet, particles were never found within cell nuclei. The presence of the respective particles within the cells was confirmed by EDX. Live-cell imaging revealed the time-dependent process of internalisation of technical nanoparticles, which was exemplified by tungsten carbide particle uptake into the human skin cells, HaCaT. Particles were found to co-localise with lysosomal structures within the cells. The incorporated nanoparticles changed the cellular granularity, as measured by flow cytometry, already after 3 h of exposure in a particle specific manner. By correlating particle properties with flow cytometry data, only the primary particle size was found to be a weakly influential property for particle uptake. CytoD, an inhibitor of actin filaments and therewith of phagocytosis, significantly inhibited the internalisation of particle uptake in only two of the seven investigated cell cultures. Our study, therefore, supports the notion that nanoparticles can enter mammalian cells quickly and easily, irrespective of the phagocytic ability of the cells.
This paper references
10.1021/NL052396O
Determining the size and shape dependence of gold nanoparticle uptake into mammalian cells.
B. Chithrani (2006)
10.1186/1743-8977-6-17
Titanium dioxide nanoparticles induce oxidative stress and DNA-adduct formation but not DNA-breakage in human lung cells
K. Bhattacharya (2008)
10.1289/ehp.8497
Induction of Inflammation in Vascular Endothelial Cells by Metal Oxide Nanoparticles: Effect of Particle Composition
A. Gojova (2007)
10.1016/j.aquatox.2007.11.019
Toxicity and bioaccumulation of xenobiotic organic compounds in the presence of aqueous suspensions of aggregates of nano-C(60).
A. Baun (2008)
10.1002/asia.200800290
Inorganic metal hydroxide nanoparticles for targeted cellular uptake through clathrin-mediated endocytosis.
J. Oh (2009)
10.1038/nrm1940
Harnessing actin dynamics for clathrin-mediated endocytosis
M. Kaksonen (2006)
10.1021/NL061025K
Comparison of the abilities of ambient and manufactured nanoparticles to induce cellular toxicity according to an oxidative stress paradigm.
T. Xia (2006)
10.1016/j.toxlet.2009.06.851
Engineered cobalt oxide nanoparticles readily enter cells.
E. Papis (2009)
10.1124/pr.54.3.431
Caveolae: From Cell Biology to Animal Physiology
B. Razani (2002)
10.1073/pnas.0608582104
Understanding the nanoparticle–protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles
T. Cedervall (2007)
10.1073/pnas.0801763105
The effect of particle design on cellular internalization pathways
Stephanie E. A. Gratton (2008)
10.1093/neuonc/2.3.174
Phosphatidylserine-dependent phagocytosis of apoptotic glioma cells by normal human microglia, astrocytes, and glioma cells.
G. H. Chang (2000)
10.4028/www.scientific.net/SSP.151.183
Evaluation of Health Risks of Nanoparticles – A Contribution to a Sustainable Development of Nanotechnology
A. Potthoff (2009)
10.1289/ehp.8006
Ultrafine Particles Cross Cellular Membranes by Nonphagocytic Mechanisms in Lungs and in Cultured Cells
M. Geiser (2005)
10.1016/J.YEXCR.2004.12.021
Formation of nucleoplasmic protein aggregates impairs nuclear function in response to SiO2 nanoparticles.
Min Chen (2005)
10.1016/0165-0173(92)90007-9
Brain macrophages: evaluation of microglia and their functions
W. E. Thomas (1992)
10.1002/CYTO.990200106
Flow cytometric assay of lung macrophage uptake of environmental particulates.
B. Stringer (1995)
10.1007/s00204-007-0178-5
Actin plays a crucial role in the phagocytosis and biological response to respirable quartz particles in macrophages
P. Haberzettl (2007)
10.1016/J.TOX.2006.10.007
Mutagenicity of diesel exhaust particles mediated by cell-particle interaction in mammalian cells.
Lingzhi Bao (2007)
10.1289/ehp.11370
Deducing in Vivo Toxicity of Combustion-Derived Nanoparticles from a Cell-Free Oxidative Potency Assay and Metabolic Activation of Organic Compounds
T. Stoeger (2009)
10.1042/BJ20031253
Size-dependent internalization of particles via the pathways of clathrin- and caveolae-mediated endocytosis.
J. Rejman (2004)
10.1074/JBC.C100613200
Caveolae-deficient Endothelial Cells Show Defects in the Uptake and Transport of Albumin in Vivo *
W. Schubert (2001)
10.1093/toxsci/kfp087
Mechanisms of quantum dot nanoparticle cellular uptake.
L. Zhang (2009)
10.1016/S0022-5320(69)90033-1
A low-viscosity epoxy resin embedding medium for electron microscopy.
A. Spurr (1969)
10.3181/00379727-207-2320107
Fine Particles That Adsorb Lipopolysaccharide Via Bridging Calcium Cations May Mimic Bacterial Pathogenicity Towards Cells
P. Ashwood (2007)
10.1080/08958370902942608
Physico-chemical characterization in the light of toxicological effects
T. Meißner (2009)
10.1007/S11051-005-6770-9
Airborne Nanostructured Particles and Occupational Health
A. Maynard (2005)
10.1016/j.envpol.2009.08.021
Evaluating the cytotoxicity of palladium/magnetite nano-catalysts intended for wastewater treatment.
H. Hildebrand (2010)
10.1093/TOXSCI/KFM050
A murine scavenger receptor MARCO recognizes polystyrene nanoparticles.
S. Kanno (2007)
10.1289/ehp.0800121
Toxicity of Tungsten Carbide and Cobalt-Doped Tungsten Carbide Nanoparticles in Mammalian Cells in Vitro
S. Bastian (2009)
10.1021/ES060589N
Titanium dioxide (P25) produces reactive oxygen species in immortalized brain microglia (BV2): implications for nanoparticle neurotoxicity.
T. Long (2006)
10.1006/JTBI.1999.0948
The physical basis of transparency in biological tissue: ultrastructure and the minimization of light scattering
Johnsen (1999)
10.1165/AJRCMB.24.2.4081
Endocytosis of ultrafine particles by A549 cells.
R. Stearns (2001)
10.1073/pnas.0805135105
Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts
M. Lundqvist (2008)
10.1128/IAI.67.7.3188-3192.1999
Effects of Mycoplasma fermentansincognitus on Differentiation of THP-1 Cells
L. Reyes (1999)
10.1152/AJPLUNG.00173.2005
Clathrin-mediated endocytosis of FITC-albumin in alveolar type II epithelial cell line RLE-6TN.
R. Yumoto (2006)
10.1289/ehp.0901615
Environment and Health in China: Challenges and Opportunities
H. Kan (2009)
10.22028/D291-24487
Colloidal processing and sintering of nano-scale TiN
R. Nass (1995)
10.1021/bm700535p
Surface charge of nanoparticles determines their endocytic and transcytotic pathway in polarized MDCK cells.
Oshrat Harush-Frenkel (2008)
Glucocorticoid regulation of in vitro astrocyte phagocytosis.
A. Roldán (1997)
10.1186/1471-2164-11-65
Tungsten carbide cobalt nanoparticles exert hypoxia-like effects on the gene expression level in human keratinocytes
W. Busch (2009)
10.3109/17435391003605455
Physical-chemical characterization of tungsten carbide nanoparticles as a basis for toxicological investigations
T. Meißner (2010)
10.1016/j.aquatox.2009.04.003
Agglomeration of tungsten carbide nanoparticles in exposure medium does not prevent uptake and toxicity toward a rainbow trout gill cell line.
D. Kühnel (2009)
10.1080/10611860290031877
Apolipoprotein-mediated Transport of Nanoparticle-bound Drugs Across the Blood-Brain Barrier
J. Kreuter (2002)
10.1021/ES051043O
Oxide nanoparticle uptake in human lung fibroblasts: effects of particle size, agglomeration, and diffusion at low concentrations.
Ludwig K. Limbach (2005)
10.1021/TX6003198
Stabilization of C60 nanoparticles by protein adsorption and its implications for toxicity studies.
S. Deguchi (2007)



This paper is referenced by
10.1186/1743-8977-8-36
Shape matters: effects of silver nanospheres and wires on human alveolar epithelial cells
Linda C. Stoehr (2011)
10.1002/term.1889
Nanoparticulate delivery of agents for induced elastogenesis in three‐dimensional collagenous matrices
L. Venkataraman (2016)
10.1039/c3nr02548a
Plant mediated green synthesis: modified approaches.
R. Das (2013)
NanocarriersEnhanceDoxorubicinUptakeinDrug-Resistant Ovarian Cancer Cells
Hans Chin Arora (2011)
Cerium oxide nanoparticles exhibit an energy-dependent protection to in-vitro cell cultures under X-ray radiation exposure
A. Briggs (2013)
10.1016/B978-0-9830791-6-3.50011-4
Role of hydrophobicity on antioxidant activity in lipid dispersions, From the polar paradox to the cut-off theory
M. Laguerre (2013)
10.1007/s11051-013-1711-5
Maturation and demise of human primary monocytes by carbon nanotubes
M. Nicola (2013)
Investigating the properties and application of tantalum pentoxide nanostructures for cancer radiotherapy
Ryan S Brown (2017)
10.1016/j.nano.2013.02.008
Cerium oxide nanoparticles: influence of the high-Z component revealed on radioresistant 9L cell survival under X-ray irradiation.
A. Briggs (2013)
10.1517/17425247.2012.745848
Ceramic nanocarriers: versatile nanosystem for protein and peptide delivery
D. Singh (2013)
10.1016/j.ab.2015.04.034
Phagocytosis-coupled flow cytometry for detection and size discrimination of anionic polystyrene particles.
Emily Mutzke (2015)
10.1158/0008-5472.CAN-11-2890
Nanocarriers enhance Doxorubicin uptake in drug-resistant ovarian cancer cells.
H. Arora (2012)
10.1016/j.scitotenv.2018.04.180
Environmental mixtures of nanomaterials and chemicals: The Trojan-horse phenomenon and its relevance for ecotoxicity.
Steffi Naasz (2018)
Controlled Delivery of TGF-β1 from PLGA Nanoparticles
P. Vaidya (2012)
10.1186/s12951-017-0254-9
Interaction of silver nanoparticles with algae and fish cells: a side by side comparison
Y. Yue (2017)
10.1039/c2nr31652h
Intrinsically green iron oxide nanoparticles? From synthesis via (eco-)toxicology to scenario modelling.
J. Filser (2013)
10.1088/2057-1976/AA56F2
Nanostructures, concentrations and energies: an ideal equation to extend therapeutic efficiency on radioresistant 9L tumour cells using Ta2O5 ceramic nanostructured particles
Ryan Brown (2017)
10.1021/nn200126a
Ce³+ ions determine redox-dependent anti-apoptotic effect of cerium oxide nanoparticles.
I. Celardo (2011)
10.1088/2057-1976/AB0232
Nonlinear plasmonic nanohybrids as probes for multimodal cell imaging and potential phototherapeutic agents
Maxime Boksebeld (2019)
10.1186/2046-1682-6-11
Kinetic effects of TiO2 fine particles and nanoparticles aggregates on the nanomechanical properties of human neutrophils assessed by force spectroscopy
Everton Luis Santos da Rosa (2013)
10.1016/B978-0-08-099408-6.00006-2
Mechanisms of Nanotoxicity
Kristin Schirmer (2014)
10.1002/etc.4147
Nanomaterials in the environment: Behavior, fate, bioavailability, and effects-An updated review.
J. Lead (2018)
10.1021/nn301669t
Interactions of amino acids and polypeptides with metal oxide nanoparticles probed by fluorescent indicator adsorption and displacement.
Sweccha Joshi (2012)
10.1007/s11051-014-2592-y
Quantification of Al2O3 nanoparticles in human cell lines applying inductively coupled plasma mass spectrometry (neb-ICP-MS, LA-ICP-MS) and flow cytometry-based methods
S. Böhme (2014)
10.1039/c6an02661c
Combined use of AFM and soft X-ray microscopy to reveal fibres' internalization in mesothelial cells.
A. Gianoncelli (2017)
10.1007/S11051-011-0585-7
Iron-dependent formation of reactive oxygen species and glutathione depletion after accumulation of magnetic iron oxide nanoparticles by oligodendroglial cells
M. Hohnholt (2011)
10.1007/978-1-62703-462-3_14
Immunocytochemistry, electron tomography, and energy dispersive X-ray spectroscopy (EDXS) on cryosections of human cancer cells doped with stimuli responsive polymeric nanogels loaded with iron oxide nanoparticles.
R. Marotta (2013)
10.1039/C4BM00221K
Combination of magnetic field and surface functionalization for reaching synergistic effects in cellular labeling by magnetic core-shell nanospheres.
Tina Gulin-Sarfraz (2014)
10.1016/j.tibtech.2016.02.005
Checking the Biocompatibility of Plant-Derived Metallic Nanoparticles: Molecular Perspectives.
R. Das (2016)
10.2147/IJN.S38749
Visualization of internalization of functionalized cobalt ferrite nanoparticles and their intracellular fate
V. B. Bregar (2013)
10.1088/0957-4484/23/46/465103
Cell uptake survey of pegylated nanographene oxide.
M. Vila (2012)
10.1002/jbm.b.33178
Cellular internalization and detailed toxicity analysis of protein-immobilized iron oxide nanoparticles.
Purva Sanganeria (2015)
See more
Semantic Scholar Logo Some data provided by SemanticScholar