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Inhibition Of Corneal Neovascularization With Endostatin Delivered By Adeno-associated Viral (AAV) Vector In A Mouse Corneal Injury Model.
L. Lai, X. Xiao, J. Wu
Published 2007 · Biology, Medicine
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The use of a recombinant adeno-associated viral (rAAV) vector carrying endostatin gene as an anti-angiogenesis strategy to treat corneal neovascularization in a mouse model was evaluated. Subconjunctival injection of recombinant endostatin-AAV was used to examine the inhibition of corneal neovascularization induced by silver nitrate cauterization in mice. The results showed that gene expression in corneal tissue was observed as early as 4 days after gene transfer and stably lasted for over 8 months with minimal immune reaction. Subconjunctival injection of a high-titer rAAV-endostatin successfully inhibited neovascularization. Immunohistchemistry staining of CD 31 and endostatin showed that the treatment significantly inhibits angiogenesis in cornea. We concluded that the rAAV was capable of directly delivering genes to the ocular surface epithelium by way of subconjunctival injection and was able to deliver sustained, high levels of gene expression in vivo to inhibit angiogenesis.
This paper references
New perspectives in clinical oncology from angiogenesis research.
J. Folkman (1996)
Angiostatin inhibits and regresses corneal neovascularization.
B. Ambati (2002)
The angiogenesis inhibitor, endostatin, does not affect murine cutaneous wound healing.
A. Berger (2000)
Steroid inhibition of limbal blood and lymphatic vascular cell growth.
G. C. Boneham (1995)
Endostatin: the logic of antiangiogenic therapy.
A. Abdollahi (2005)
Requirement for vascular endothelial growth factor in wound- and inflammation-related corneal neovascularization.
S. Amano (1998)
Phase I pharmacokinetic and pharmacodynamic study of recombinant human endostatin in patients with advanced solid tumors.
J. Thomas (2003)
Inducible adeno-associated virus vector-delivered transgene expression in corneal endothelium.
M. Tsai (2002)
Immunohistochemical Localization of Vascular Endothelial Growth Factor, Transforming Growth Factor &agr;, and Transforming Growth Factor &bgr;1 in Human Corneas with Neovascularization
C. Cursiefen (2000)
Inhibition of retinal neovascularization by intraocular viral-mediated delivery of anti-angiogenic agents.
A. Auricchio (2002)
AN ANIMAL MODEL FOR CORNEAL GRAFT REJECTION IN HIGH‐RISK KERATOPLASTY
J. Hill (1988)
Circular Intermediates of Recombinant Adeno-Associated Virus Have Defined Structural Characteristics Responsible for Long-Term Episomal Persistence in Muscle Tissue
D. Duan (1998)
Corneal neovascularization induced by xenografts or chemical cautery. Inhibition by cyclosporin A.
U. Benelli (1997)
Adeno-associated virus vector gene expression occurs in nondividing cells in the absence of vector DNA integration.
T. Flotte (1994)
Adeno-associated virus serotype 8 efficiently delivers genes to muscle and heart
Z. Wang (2005)
Inhibition of rat corneal angiogenesis by 16-kDa prolactin and by endogenous prolactin-like molecules.
Z. Dueñas (1999)
The antiangiogenic and therapeutic implications of endostatin.
B. Ren (2003)
Topical modulation of interleukin-1 activity in corneal neovascularization.
M. R. Dana (1998)
Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells.
R. J. Tsai (2000)
Intratumoral gene therapy of malignant brain tumor in a rat model with angiostatin delivered by adeno-associated viral (AAV) vector
H-I Ma (2002)
Retroviral endostatin gene transfer inhibits growth of human lung cancer in a murine orthotopic xenotransplant model
R. Kurdow (2003)
Effects of thalidomide and related metabolites in a mouse corneal model of neovascularization.
B. Kenyon (1997)
Effects of 1alpha,25-dihydroxyvitamin D3 on Langerhans cell migration and corneal neovascularization in mice.
T. Suzuki (2000)
Recombinant human endostatin administered as a 28-day continuous intravenous infusion, followed by daily subcutaneous injections: a phase I and pharmacokinetic study in patients with advanced cancer.
A. H. G. Hansma (2005)
Endostatin: An Endogenous Inhibitor of Angiogenesis and Tumor Growth
M. O'reilly (1997)
Long-term gene expression and phenotypic correction using adeno-associated virus vectors in the mammalian brain
M. Kaplitt (1994)
Topical formulations of novel angiostatic steroids inhibit rabbit corneal neovascularization.
D. Benezra (1997)
Inhibition of vascular endothelial cell morphogenesis in cultures by limbal epithelial cells.
D. Ma (1999)
Adenovirus-mediated gene transfer to the ocular surface epithelium.
K. Tsubota (1998)
Efficient long-term gene transfer into muscle tissue of immunocompetent mice by adeno-associated virus vector.
X. Xiao (1996)
Synergistic antitumor effect of antiangiogenic factor genes on colon 26 produced by low-voltage electroporation
M. Uesato (2004)
Effect of human recombinant Endostatin® protein on human angiogenesis
S. P. Jung (2004)
Self-complementary recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently of DNA synthesis
DM McCarty (2001)
Inhibition of tumor growth in xenografted nude mice with adenovirus-mediated endostatin gene comparison with recombinant endostatin protein.
Z. Liang (2004)
Fluorescein-Potentiated Argon Laser Therapy Improves Symptoms and Appearance of Corneal Neovascularization
Y. J. Gordon (2002)
Inhibition of inflammatory corneal angiogenesis by TNP-470.
A. Joussen (2001)
Inhibition of arthritis by systemic administration of endostatin in passive murine collagen induced arthritis
D. Kurosaka (2003)
Adeno-associated virus vector carrying human minidystrophin genes effectively ameliorates muscular dystrophy in mdx mouse model.
B. Wang (2000)
Inhibition of angiogenesis by adenovirus-mediated sFlt-1 expression in a rat model of corneal neovascularization.
C. Lai (2001)
Angiogenesis: regulators and clinical applications.
S. Liekens (2001)
Synergistic anti-tumor effect of recombinant human endostatin adenovirus combined with gemcitabine
Y. Wu (2005)
Suppression of intracranial human glioma growth after intramuscular administration of an adeno-associated viral vector expressing angiostatin.
Hsin-I Ma (2002)
Production of High-Titer Recombinant Adeno-Associated Virus Vectors in the Absence of Helper Adenovirus
X. Xiao (1998)
rAAV vector-mediated sarcogylcan gene transfer in a hamster model for limb girdle muscular dystrophy
J. Li (1999)
Photodynamic Therapy of Corneal Neovascularization with Verteporfin
M. Fossarello (2003)
Enhancement of radiation effects by pXLG-mEndo in a lung carcinoma model.
Xian Luo (2005)
Widespread and stable pancreatic gene transfer by adeno-associated virus vectors via different routes.
Z. Wang (2006)
This paper is referenced by
Imagerie et quantification de la néovascularisation cornéenne
Y. Benayoun (2013)
Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver.
M. Mansour (2011)
Corneal gene therapy: basic science and translational perspective.
R. Mohan (2013)
Transduction efficiency of AAV 2/6, 2/8 and 2/9 vectors for delivering genes in human corneal fibroblasts
A. Sharma (2010)
[Corneal neovascularization: epidemiological, physiopathological, and clinical features].
Y. Benayoun (2013)
Targeting Herpetic Keratitis by Gene Therapy
H. Elbadawy (2012)
[Imaging and quantification of corneal neovascularization].
Y. Benayoun (2013)
A Possible Antineoplastic Potential of Selective, Irreversible Proteasome Inhibitor, Carfilzomib on Chemically Induced Hepatocarcinogenesis in Rats
M. Mansour (2014)
Angiogenesis from a new perspective
Beatrice Peebo (2012)
Selection and characterization of targeted vector capsids from random adeno-associated virus type 2 (AAV-2) display peptide libraries
S. Michelfelder (2008)
Down-regulation of vascular endothelial growth factor and up-regulation of pigment epithelium derived factor make low molecular weight heparin-endostatin and polyethylene glycol-endostatin potential candidates for anti-angiogenesis drug.
H. Tan (2011)
Ocular gene therapy: an evaluation of recombinant adeno-associated virus-mediated gene therapy interventions for the treatment of ocular disease.
Kamolika Roy (2010)
A comprehensive review of retinal gene therapy.
S. Boye (2013)
Corneal Neovascularization as a Target for Nucleotide-Based Therapies
Yi-Jen Hsueh (2015)
VesselJ: A New Tool for Semiautomatic Measurement of Corneal Neovascularization.
A. Rabiolo (2015)
Corneal Neovascularization: A Translational Perspective
Ferrari Giulio (2015)
Horizons in therapy for corneal angiogenesis.
Surekha Maddula (2011)
Néovascularisation cornéenne : aspects épidémiologiques, physiopathologiques et cliniques
Y. Benayoun (2013)
The vignette for V13N3 issue
Michael M. C. Lai (2006)
Nanoparticle-mediated delivery of shRNA.VEGF-a plasmids regresses corneal neovascularization.
Y. Qazi (2012)
Multi-gene targeted antiangiogenic therapies for experimental corneal neovascularization
P. Chen (2010)
Gene therapy approaches to disease of the cornea and anterior chamber
M. Basche (2014)
Possible role of selective, irreversible, proteasome inhibitor (carfilzomib) in the treatment of rat hepatocellular carcinoma.
M. Mansour (2014)
Cornea Prevention of Corneal Neovascularization by Adenovirus Encoding Human Vascular Endothelial Growth Factor Soluble Receptor ( s-VEGFR 1 ) in Lacrimal Gland
L. F. Nominato (2018)
Gene-based antiangiogenic applications for corneal neovascularization.
Siyin Liu (2018)
The Management of Corneal Neovascularisation – Update on New Clinical Data and Recommendations of Treatment
N. Spiteri (2016)
Immunological Disruption of Antiangiogenic Signals by Recruited Allospecific T Cells Leads to Corneal Allograft Rejection
Y. Tan (2012)
Gene Therapy in Retinal Dystrophies
L. Ziccardi (2019)
Collagens and proteoglycans of the cornea: importance in transparency and visual disorders
D. Massoudi (2015)
Corneal neovascularization: a review of the molecular biology and current therapies
Michael L Rolfsen (2013)
Bioadhesive hyaluronan–chitosan nanoparticles can transport genes across the ocular mucosa and transfect ocular tissue
M. Fuente (2008)
Inhibitory effect of polysulfated heparin endostatin on alkali burn induced corneal neovascularization in rabbits.
Zhao-na Li (2015)See more