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Inhibition Of Corneal Neovascularization With Endostatin Delivered By Adeno-associated Viral (AAV) Vector In A Mouse Corneal Injury Model.

L. Lai, X. Xiao, J. Wu
Published 2007 · Biology, Medicine

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The use of a recombinant adeno-associated viral (rAAV) vector carrying endostatin gene as an anti-angiogenesis strategy to treat corneal neovascularization in a mouse model was evaluated. Subconjunctival injection of recombinant endostatin-AAV was used to examine the inhibition of corneal neovascularization induced by silver nitrate cauterization in mice. The results showed that gene expression in corneal tissue was observed as early as 4 days after gene transfer and stably lasted for over 8 months with minimal immune reaction. Subconjunctival injection of a high-titer rAAV-endostatin successfully inhibited neovascularization. Immunohistchemistry staining of CD 31 and endostatin showed that the treatment significantly inhibits angiogenesis in cornea. We concluded that the rAAV was capable of directly delivering genes to the ocular surface epithelium by way of subconjunctival injection and was able to deliver sustained, high levels of gene expression in vivo to inhibit angiogenesis.
This paper references
10.1016/S0959-8049(96)00423-6
New perspectives in clinical oncology from angiogenesis research.
J. Folkman (1996)
10.1001/ARCHOPHT.120.8.1063
Angiostatin inhibits and regresses corneal neovascularization.
B. Ambati (2002)
10.1006/JSRE.2000.5890
The angiogenesis inhibitor, endostatin, does not affect murine cutaneous wound healing.
A. Berger (2000)
10.3109/02713689508999908
Steroid inhibition of limbal blood and lymphatic vascular cell growth.
G. C. Boneham (1995)
10.1016/J.DRUP.2005.03.001
Endostatin: the logic of antiangiogenic therapy.
A. Abdollahi (2005)
Requirement for vascular endothelial growth factor in wound- and inflammation-related corneal neovascularization.
S. Amano (1998)
10.1200/JCO.2003.12.120
Phase I pharmacokinetic and pharmacodynamic study of recombinant human endostatin in patients with advanced solid tumors.
J. Thomas (2003)
Inducible adeno-associated virus vector-delivered transgene expression in corneal endothelium.
M. Tsai (2002)
10.1097/00003226-200007000-00025
Immunohistochemical Localization of Vascular Endothelial Growth Factor, Transforming Growth Factor &agr;, and Transforming Growth Factor &bgr;1 in Human Corneas with Neovascularization
C. Cursiefen (2000)
10.1006/MTHE.2002.0702
Inhibition of retinal neovascularization by intraocular viral-mediated delivery of anti-angiogenic agents.
A. Auricchio (2002)
10.1097/00007890-198807000-00003
AN ANIMAL MODEL FOR CORNEAL GRAFT REJECTION IN HIGH‐RISK KERATOPLASTY
J. Hill (1988)
10.1128/JVI.72.11.8568-8577.1998
Circular Intermediates of Recombinant Adeno-Associated Virus Have Defined Structural Characteristics Responsible for Long-Term Episomal Persistence in Muscle Tissue
D. Duan (1998)
Corneal neovascularization induced by xenografts or chemical cautery. Inhibition by cyclosporin A.
U. Benelli (1997)
10.1165/AJRCMB.11.5.7946381
Adeno-associated virus vector gene expression occurs in nondividing cells in the absence of vector DNA integration.
T. Flotte (1994)
10.1038/nbt1073
Adeno-associated virus serotype 8 efficiently delivers genes to muscle and heart
Z. Wang (2005)
Inhibition of rat corneal angiogenesis by 16-kDa prolactin and by endogenous prolactin-like molecules.
Z. Dueñas (1999)
10.1358/MF.2003.25.3.769643
The antiangiogenic and therapeutic implications of endostatin.
B. Ren (2003)
10.1097/00003226-199807000-00011
Topical modulation of interleukin-1 activity in corneal neovascularization.
M. R. Dana (1998)
10.1056/NEJM200007133430202
Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells.
R. J. Tsai (2000)
10.1038/sj.gt.3301616
Intratumoral gene therapy of malignant brain tumor in a rat model with angiostatin delivered by adeno-associated viral (AAV) vector
H-I Ma (2002)
10.1007/s00423-003-0400-8
Retroviral endostatin gene transfer inhibits growth of human lung cancer in a murine orthotopic xenotransplant model
R. Kurdow (2003)
10.1006/EXER.1997.0292
Effects of thalidomide and related metabolites in a mouse corneal model of neovascularization.
B. Kenyon (1997)
Effects of 1alpha,25-dihydroxyvitamin D3 on Langerhans cell migration and corneal neovascularization in mice.
T. Suzuki (2000)
10.1093/ANNONC/MDI318
Recombinant human endostatin administered as a 28-day continuous intravenous infusion, followed by daily subcutaneous injections: a phase I and pharmacokinetic study in patients with advanced cancer.
A. H. G. Hansma (2005)
10.1016/S0092-8674(00)81848-6
Endostatin: An Endogenous Inhibitor of Angiogenesis and Tumor Growth
M. O'reilly (1997)
10.1038/NG1094-148
Long-term gene expression and phenotypic correction using adeno-associated virus vectors in the mammalian brain
M. Kaplitt (1994)
Topical formulations of novel angiostatic steroids inhibit rabbit corneal neovascularization.
D. Benezra (1997)
Inhibition of vascular endothelial cell morphogenesis in cultures by limbal epithelial cells.
D. Ma (1999)
10.1006/EXER.1998.0557
Adenovirus-mediated gene transfer to the ocular surface epithelium.
K. Tsubota (1998)
10.1128/JVI.70.11.8098-8108.1996
Efficient long-term gene transfer into muscle tissue of immunocompetent mice by adeno-associated virus vector.
X. Xiao (1996)
10.1038/sj.cgt.7700740
Synergistic antitumor effect of antiangiogenic factor genes on colon 26 produced by low-voltage electroporation
M. Uesato (2004)
10.1023/A:1021540328613
Effect of human recombinant Endostatin® protein on human angiogenesis
S. P. Jung (2004)
10.1038/sj.gt.3301514
Self-complementary recombinant adeno-associated virus (scAAV) vectors promote efficient transduction independently of DNA synthesis
DM McCarty (2001)
10.1016/J.YMTHE.2005.07.562
Inhibition of tumor growth in xenografted nude mice with adenovirus-mediated endostatin gene comparison with recombinant endostatin protein.
Z. Liang (2004)
10.1097/00003226-200211000-00007
Fluorescein-Potentiated Argon Laser Therapy Improves Symptoms and Appearance of Corneal Neovascularization
Y. J. Gordon (2002)
Inhibition of inflammatory corneal angiogenesis by TNP-470.
A. Joussen (2001)
10.1136/ard.62.7.677
Inhibition of arthritis by systemic administration of endostatin in passive murine collagen induced arthritis
D. Kurosaka (2003)
10.1073/PNAS.240335297
Adeno-associated virus vector carrying human minidystrophin genes effectively ameliorates muscular dystrophy in mdx mouse model.
B. Wang (2000)
10.1089/104303401750270959
Inhibition of angiogenesis by adenovirus-mediated sFlt-1 expression in a rat model of corneal neovascularization.
C. Lai (2001)
10.1016/S0006-2952(00)00529-3
Angiogenesis: regulators and clinical applications.
S. Liekens (2001)
10.1097/00001813-200506000-00011
Synergistic anti-tumor effect of recombinant human endostatin adenovirus combined with gemcitabine
Y. Wu (2005)
Suppression of intracranial human glioma growth after intramuscular administration of an adeno-associated viral vector expressing angiostatin.
Hsin-I Ma (2002)
10.1128/JVI.72.3.2224-2232.1998
Production of High-Titer Recombinant Adeno-Associated Virus Vectors in the Absence of Helper Adenovirus
X. Xiao (1998)
10.1038/sj.gt.3300830
rAAV vector-mediated sarcogylcan gene transfer in a hamster model for limb girdle muscular dystrophy
J. Li (1999)
10.1097/00003226-200307000-00018
Photodynamic Therapy of Corneal Neovascularization with Verteporfin
M. Fossarello (2003)
10.1016/J.IJROBP.2005.01.038
Enhancement of radiation effects by pXLG-mEndo in a lung carcinoma model.
Xian Luo (2005)
10.2337/DIABETES.55.04.06.DB05-0927
Widespread and stable pancreatic gene transfer by adeno-associated virus vectors via different routes.
Z. Wang (2006)



This paper is referenced by
10.1016/J.JFO.2013.04.006
Imagerie et quantification de la néovascularisation cornéenne
Y. Benayoun (2013)
10.1016/j.lfs.2011.06.002
Comparison of angiotensin converting enzyme inhibitors and angiotensin II type 1 receptor blockade for the prevention of premalignant changes in the liver.
M. Mansour (2011)
10.1016/j.jtos.2012.10.004
Corneal gene therapy: basic science and translational perspective.
R. Mohan (2013)
10.1016/j.brainresbull.2009.07.005
Transduction efficiency of AAV 2/6, 2/8 and 2/9 vectors for delivering genes in human corneal fibroblasts
A. Sharma (2010)
10.1016/j.jfo.2013.03.002
[Corneal neovascularization: epidemiological, physiopathological, and clinical features].
Y. Benayoun (2013)
10.1155/2012/594869
Targeting Herpetic Keratitis by Gene Therapy
H. Elbadawy (2012)
10.1016/j.jfo.2013.04.006
[Imaging and quantification of corneal neovascularization].
Y. Benayoun (2013)
10.1002/jbt.21577
A Possible Antineoplastic Potential of Selective, Irreversible Proteasome Inhibitor, Carfilzomib on Chemically Induced Hepatocarcinogenesis in Rats
M. Mansour (2014)
Angiogenesis from a new perspective
Beatrice Peebo (2012)
Selection and characterization of targeted vector capsids from random adeno-associated virus type 2 (AAV-2) display peptide libraries
S. Michelfelder (2008)
10.1248/BPB.34.545
Down-regulation of vascular endothelial growth factor and up-regulation of pigment epithelium derived factor make low molecular weight heparin-endostatin and polyethylene glycol-endostatin potential candidates for anti-angiogenesis drug.
H. Tan (2011)
10.1089/hum.2010.041
Ocular gene therapy: an evaluation of recombinant adeno-associated virus-mediated gene therapy interventions for the treatment of ocular disease.
Kamolika Roy (2010)
10.1038/mt.2012.280
A comprehensive review of retinal gene therapy.
S. Boye (2013)
10.4172/2155-9570.1000409
Corneal Neovascularization as a Target for Nucleotide-Based Therapies
Yi-Jen Hsueh (2015)
10.1167/iovs.15-17098
VesselJ: A New Tool for Semiautomatic Measurement of Corneal Neovascularization.
A. Rabiolo (2015)
10.4172/2155-9570.1000387
Corneal Neovascularization: A Translational Perspective
Ferrari Giulio (2015)
10.1016/j.ophtha.2011.01.041
Horizons in therapy for corneal angiogenesis.
Surekha Maddula (2011)
10.1016/J.JFO.2013.03.002
Néovascularisation cornéenne : aspects épidémiologiques, physiopathologiques et cliniques
Y. Benayoun (2013)
10.1007/s11373-006-9074-x
The vignette for V13N3 issue
Michael M. C. Lai (2006)
10.1167/iovs.11-9139
Nanoparticle-mediated delivery of shRNA.VEGF-a plasmids regresses corneal neovascularization.
Y. Qazi (2012)
Multi-gene targeted antiangiogenic therapies for experimental corneal neovascularization
P. Chen (2010)
Gene therapy approaches to disease of the cornea and anterior chamber
M. Basche (2014)
10.1016/j.cbi.2014.03.001
Possible role of selective, irreversible, proteasome inhibitor (carfilzomib) in the treatment of rat hepatocellular carcinoma.
M. Mansour (2014)
Cornea Prevention of Corneal Neovascularization by Adenovirus Encoding Human Vascular Endothelial Growth Factor Soluble Receptor ( s-VEGFR 1 ) in Lacrimal Gland
L. F. Nominato (2018)
10.1016/j.survophthal.2017.10.006
Gene-based antiangiogenic applications for corneal neovascularization.
Siyin Liu (2018)
10.17925/EOR.2016.10.02.86
The Management of Corneal Neovascularisation – Update on New Clinical Data and Recommendations of Treatment
N. Spiteri (2016)
10.4049/jimmunol.1103216
Immunological Disruption of Antiangiogenic Signals by Recruited Allospecific T Cells Leads to Corneal Allograft Rejection
Y. Tan (2012)
10.3390/ijms20225722
Gene Therapy in Retinal Dystrophies
L. Ziccardi (2019)
10.1007/s00441-015-2233-5
Collagens and proteoglycans of the cornea: importance in transparency and visual disorders
D. Massoudi (2015)
10.1586/eop.13.8
Corneal neovascularization: a review of the molecular biology and current therapies
Michael L Rolfsen (2013)
10.1038/gt.2008.16
Bioadhesive hyaluronan–chitosan nanoparticles can transport genes across the ocular mucosa and transfect ocular tissue
M. Fuente (2008)
10.3980/j.issn.2222-3959.2015.02.04
Inhibitory effect of polysulfated heparin endostatin on alkali burn induced corneal neovascularization in rabbits.
Zhao-na Li (2015)
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