Online citations, reference lists, and bibliographies.
← Back to Search

Identification Of Genes Associated With Cotyledon Senescence In Upland Cotton

Shen Fa-fu, Yu Shu-xun, Xie Qingen, Han Xiu-lan, F. Shu-li
Published 2006 · Biology

Cite This
Download PDF
Analyze on Scholarcy
Share
In order to unravel the biochemical pathways and understand the molecular mechanisms involved in leaf senescence, suppression subtractive hybridization (SSH) was used to generate a cDNA library enriched for transcripts differentially expressed in developmental senescence cotyledons of upland cotton. After differential screening by membrane-based hybridization and subsequent confirmation by reverse Northern blot analysis, selected 678 clones were sequenced and analyzed. Sequencing of these cDNA fragments reveals that 216 of expressed sequence tags (ESTs) represented unique genes. Of these 216 cDNAs, 151 clones (69.9%) show significant homologies to previously known genes, while the remaining 65 do not match any known sequences. 151 unique ESTs are assigned to twelve different categories based on their putative functions generated by BLAST analysis. These SAG-encoded proteins are likely to participate in macromolecule degradation, nutrient recycling, detoxification of oxidative metabolites, and signaling and regulatory events. The expression pattern of selection of genes was confirmed using northern hybridization. Northern hybridization confirmed several distinct patterns, from expression at a very early stage to the terminal phase of the senescence syndrome. Clones encoding proteases and proteins involved in macromolecule degradation and gluconeogenesis, as well as stress-related genes, are up regulated in senescence cotyledons.
This paper references
10.1046/J.1365-3040.2002.00851.X
Direct evidence for non‐enzymatic fragmentation of chloroplastic glutamine synthetase by a reactive oxygen species
H. Ishida (2002)
10.1111/J.0031-9317.2004.00314.X
Senescence and hyperspectral reflectance of cotton leaves exposed to ultraviolet-B radiation and carbon dioxide.
V. Kakani (2004)
10.1023/A:1006133311402
Markers for hypersensitive response and senescence show distinct patterns of expression
D. Pontier (2004)
10.1111/J.1365-3040.2003.01158.X
Transcriptome of Arabidopsis leaf senescence
Yongfeng Guo (2004)
10.1111/J.1365-313X.2004.02160.X
Molecular events in senescing Arabidopsis leaves.
J. Lin (2004)
10.1093/JEXBOT/53.370.927
Nitrogen metabolism and remobilization during senescence.
S. Hörtensteiner (2002)
10.1360/982004-401
Cloning and characterization of a gene encoding cysteine proteases from senescent leaves of Gossypium hirsutum
Fafu Shen (2004)
10.1104/pp.104.3.815
Iron: Nutritious, Noxious, and Not Readily Available
M. L. Guerinot (1994)
10.1104/pp.113.2.313
Making Sense of Senescence (Molecular Genetic Regulation and Manipulation of Leaf Senescence)
S. Gan (1997)
10.1073/PNAS.93.12.6025
Suppression subtractive hybridization: a method for generating differentially regulated or tissue-specific cDNA probes and libraries.
L. Diatchenko (1996)
10.1046/J.1467-7652.2003.00004.X
The molecular analysis of leaf senescence--a genomics approach.
V. Buchanan-Wollaston (2003)
10.1093/JEXBOT/52.359.1367
Ultraviolet-B exposure leads to up-regulation of senescence-associated genes in Arabidopsis thaliana.
C. John (2001)
10.1016/0168-9452(91)90199-I
Metabolism of radiolabelled galactolipids in senescent barley leaves
Leo Wanner (1991)
10.1023/A:1004652728420
Premature senescence of cotton (Gossypium hirsutum L.) – Predominantly a potassium disorder caused by an imbalance of source and sink
P. Wright (2004)
10.1104/PP.24.1.1
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.
D. Arnon (1949)
10.1104/PP.120.4.1015
Senescence-associated gene expression during ozone-induced leaf senescence in Arabidopsis.
J. D. Miller (1999)
10.1104/pp.012732
Gene Expression in Autumn Leaves1
R. Bhalerao (2003)
Cloning and characterization of a gene encoding cysteine proteases from senescent leaves of Gossypium hirsutum
SHENFafu (2004)
10.1007/BF00397663
Apparent induction of key enzymes of the glyoxylic acid cycle in senescent barley leaves
H. Gut (2004)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar