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Factors Affecting The Expression Of Foreign Proteins InEscherichia Coli
B. Glick, G. K. Whitney
Published 2005 · Biology
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SummaryA variety of factors affect the expression of foreign proteins inEscherichia coli. These include: promoter strength, efficiency of ribosome binding, stability of the foreign protein inE. coli, location of the foreign protein inE. coli, the codons used to encode the foreign protein, the metabolic state of the cell, and the location, stability and copy number of the foreign gene. This paper contains a critical review of these factors with the idea that a detailed understanding of them is the key to the development of strategies for the efficient large-scale production of foreign proteins inE. coli.
This paper references
Role of mRNA translational efficiency in bovine growth hormone expression in Escherichia coli.
B. Schoner (1984)
Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda.
E. Remaut (1981)
Cloning multiple copies of a DNA segment.
J. Hartley (1981)
Identification of a positive retroregulator that stabilizes mRNAs in bacteria.
H. C. Wong (1986)
Secretion cloning vectors in Escherichia coli.
J. Ghrayeb (1984)
A family of cloning vectors containing the lacUV5 promoter.
F. Fuller (1982)
Kinetic study of instability of recombinant plasmid pPLc23trpAl in E. coli using two‐stage continuous culture system
R. Siegel (1985)
Use of the lambda
Increased synthesis in E. coli of fibroblast and leukocyte interferons through alterations in ribosome binding sites.
H. Shepard (1982)
Versatile cloning vectors derived from the runaway-replication plasmid pKN402.
M. Bittner (1981)
Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes.
H. Grosjean (1982)
Generation of B-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli
K. Nagai (1984)
Versatile expression vectors for high-level synthesis of cloned gene products in Escherichia coli.
R. Crowl (1985)
A family of cloning vectors containing the lac UV5
F. Fuller (1982)
Isolation and Purification of Protein Granules from Escherichia coli Cells Overproducing Bovine Growth Hormone
R. Schoner (1985)
Codon usage can affect efficiency of translation of genes in Escherichia coli.
M. Robinson (1984)
High expression of cloned genes in E. coli and its consequences
M. J. Carrier (1983)
Construction of a general vector for efficient expression of mammalian proteins in bacteria: use of a synthetic ribosome binding site.
G. Jay (1981)
Stability of a plasmid F Trim in populations of a recombination-deficient strain of Escherichia coli in continuous culture
R. E. Ashby (2004)
Plasmids with temperature-dependent copy number for amplification of cloned genes and their products.
B. E. Uhlin (1979)
Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication.
E. Remaut (1983)
Expression in Escherichia coli of chemically synthesized gene for the human immune interferon.
S. Tanaka (1983)
Construction of expression plasmids producing high levels of human leukocyte-type interferon in Escherichia coli.
E. Dworkin-Rastl (1983)
Construction of plasmids carrying the cI gene of bacteriophage lambda.
K. Backman (1976)
Periplasmic production of correctly processed human growth hormone in Escherichia coli: natural and bacterial signal sequences are interchangeable.
G. Gray (1985)
F'-coded, temperature-sensitive lambda cI857 repressor gene for easy construction and regulation of lambda promoter-dependent expression systems.
M. Mieschendahl (1985)
Characterization of translational initiation sites in E. coli.
G. Stormo (1982)
Cloning with tandem gene systems for high level gene expression.
N. Lee (1984)
Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.
K. Itakura (1977)
Continuous process for production, recovery and purification of rDNA products
C. W. Robinson (1984)
Codon usage in bacteria: correlation with gene expressivity.
M. Gouy (1982)
Evidence for use of rare codons in the dnaG gene and other regulatory genes of Escherichia coli.
W. Konigsberg (1983)
Multiple joined genes prevent product degradation in Escherichia coli.
S. Shen (1984)
Large-scale preparation of ribulosebisphosphate carboxylase from a recombinant system in Escherichia coli characterized by extreme plasmid instability.
J. Pierce (1985)
New approach to tryptophan production by Escherichia coli: genetic manipulation of composite plasmids in vitro.
S. Aiba (1982)
281 cloned gene products in Escherichia coll. Gene
H. A. De Boer (1983)
Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion.
C. Hoffman (1985)
Alterations upstream from the Shine-Dalgarno region and their effect on bacterial gene expression.
P. Stanssens (1985)
 Use of the λ phage promoter PL to promote gene expression in hybrid plasmid cloning vehicles
H. Bernard (1979)
Expression vehicles used in recombinant DNA technology.
J. Friesen (1983)
Stabilization of a degradable protein by its overexpression in Escherichia coli.
Y. Cheng (1981)
An operational strategy for unstable recombinant DNA cultures
D. Ryder (1984)
Generation of β-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli
K. Nagai (1984)
Studying promoters and terminators by gene fusion.
M. Rosenberg (1983)
A PERSPECTIVE ON THE APPLICATION OF GENETIC ENGINEERING: STABILITY OF RECOMBINANT PLASMID
T. Imanaka (1981)
A technique for integrating any DNA fragment into the chromosome of Escherichia coli.
O. Raibaud (1984)
Bacteria mature preproinsulin to proinsulin.
K. Talmadge (1980)
The tac promoter: a functional hybrid derived from the trp and lac promoters.
H. D. de Boer (1983)
Molecular cloning of the DNA ligase gene from bacteriophage T4. II. Amplification and preparation of the gene product.
N. Murray (1979)
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Bacterial expression and antibiotic activities of recombinant variants of human β-defensins on pathogenic bacteria and M. tuberculosis.
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