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Factors Affecting The Expression Of Foreign Proteins InEscherichia Coli

B. Glick, G. K. Whitney
Published 2005 · Biology

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SummaryA variety of factors affect the expression of foreign proteins inEscherichia coli. These include: promoter strength, efficiency of ribosome binding, stability of the foreign protein inE. coli, location of the foreign protein inE. coli, the codons used to encode the foreign protein, the metabolic state of the cell, and the location, stability and copy number of the foreign gene. This paper contains a critical review of these factors with the idea that a detailed understanding of them is the key to the development of strategies for the efficient large-scale production of foreign proteins inE. coli.
This paper references
10.1016/0378-1119(81)90106-2
Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda.
E. Remaut (1981)
10.1093/nar/12.17.6663
Codon usage can affect efficiency of translation of genes in Escherichia coli.
M. Robinson (1984)
10.1073/pnas.82.15.5107
Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion.
C. Hoffman (1985)
A family of cloning vectors containing the lac UV5
F. Fuller (1982)
Generation of B-globin by sequence-specific proteolysis of a hybrid protein produced in Escherichia coli
K. Nagai (1984)
10.1016/0378-1119(83)90069-0
Improved plasmid vectors with a thermoinducible expression and temperature-regulated runaway replication.
E. Remaut (1983)
Use of the lambda
Bernard (1979)
10.1016/0378-1119(84)90183-5
A technique for integrating any DNA fragment into the chromosome of Escherichia coli.
O. Raibaud (1984)
10.1073/pnas.83.10.3233
Identification of a positive retroregulator that stabilizes mRNAs in bacteria.
H. C. Wong (1986)
10.1016/0378-1119(81)90014-7
Cloning multiple copies of a DNA segment.
J. Hartley (1981)
10.1002/j.1460-2075.1984.tb02151.x
Secretion cloning vectors in Escherichia coli.
J. Ghrayeb (1984)
10.1073/pnas.81.17.5403
Role of mRNA translational efficiency in bovine growth hormone expression in Escherichia coli.
B. Schoner (1984)
10.1128/jb.164.3.1366-1369.1985
F'-coded, temperature-sensitive lambda cI857 repressor gene for easy construction and regulation of lambda promoter-dependent expression systems.
M. Mieschendahl (1985)
10.1016/0378-1119(82)90187-1
A family of cloning vectors containing the lacUV5 promoter.
F. Fuller (1982)
10.1002/bit.260270105
Kinetic study of instability of recombinant plasmid pPLc23trpAl in E. coli using two‐stage continuous culture system
R. Siegel (1985)
10.1016/0378-1119(85)90319-1
Periplasmic production of correctly processed human growth hormone in Escherichia coli: natural and bacterial signal sequences are interchangeable.
G. Gray (1985)
10.1016/0378-1119(83)90007-0
Construction of expression plasmids producing high levels of human leukocyte-type interferon in Escherichia coli.
Eva Dworkin-Rastl (1983)
10.1007/BF00400172
Stability of a plasmid F Trim in populations of a recombination-deficient strain of Escherichia coli in continuous culture
Robert E. Ashby (1984)
10.1073/pnas.78.9.5543
Construction of a general vector for efficient expression of mammalian proteins in bacteria: use of a synthetic ribosome binding site.
G. Jay (1981)
10.1111/j.1749-6632.1981.tb14172.x
A perspective on the application of genetic engineering: stability of recombinant plasmid.
T. Imanaka (1981)
10.1126/science.6356355
Studying promoters and terminators by gene fusion.
M. Rosenberg (1983)
10.1128/aem.49.5.1094-1100.1985
Large-scale preparation of ribulosebisphosphate carboxylase from a recombinant system in Escherichia coli characterized by extreme plasmid instability.
J. Pierce (1985)
281 cloned gene products in Escherichia coll. Gene
H. A. De Boer (1983)
10.1002/bit.260260819
An operational strategy for unstable recombinant DNA cultures
D. F. Ryder (1984)
10.1016/0378-1119(81)90154-2
Stabilization of a degradable protein by its overexpression in Escherichia coli.
Y. Cheng (1981)
10.1016/0734-9750(83)90589-X
Expression vehicles used in recombinant DNA technology.
J. Friesen (1983)
10.1016/0378-1119(81)90175-X
Versatile cloning vectors derived from the runaway-replication plasmid pKN402.
M. Bittner (1981)
10.1016/0378-1119(82)90157-3
Preferential codon usage in prokaryotic genes: the optimal codon-anticodon interaction energy and the selective codon usage in efficiently expressed genes.
H. Grosjean (1982)
10.1126/science.412251
Expression in Escherichia coli of a chemically synthesized gene for the hormone somatostatin.
K. Itakura (1977)
Continuous process for production, recovery and purification of rDNA products
C. W. Robinson (1984)
10.1093/nar/12.17.6797
Cloning with tandem gene systems for high level gene expression.
N. Lee (1984)
10.1093/nar/10.22.7055
Codon usage in bacteria: correlation with gene expressivity.
M. Gouy (1982)
10.1073/pnas.80.3.687
Evidence for use of rare codons in the dnaG gene and other regulatory genes of Escherichia coli.
W. Konigsberg (1983)
10.1073/pnas.81.15.4627
Multiple joined genes prevent product degradation in Escherichia coli.
S. Shen (1984)



This paper is referenced by
10.3390/biom8040146
Induction of Recombinant Lectin Expression by an Artificially Constructed Tandem Repeat Structure: A Case Study Using Bryopsis plumosa Mannose-Binding Lectin
Hyun-ju Hwang (2018)
10.1016/j.pep.2019.105539
Improving the heterologous expression of human β-defensin 2 (HBD2) using an experimental design.
L. Corrales-Garcia (2019)
Development of Biofilm of Escherichia Coli with Subunits of Aeromonas Hydrophila
B. T. Naveen Kumar (2014)
10.1007/BF01569997
Review: Optimizing inducer and culture conditions for expression of foreign proteins under the control of thelac promoter
R. Donovan (2005)
10.1007/BF01568932
Effect of transformation ofAzotobacter vinelandii with the low copy number plasmid pRK290
B. Glick (2005)
10.5402/2013/590587
Critical Factors Affecting the Success of Cloning, Expression, and Mass Production of Enzymes by Recombinant E. coli
M. Fakruddin (2013)
10.1080/10826068.2017.1342259
Optimization of an anti-HER2 nanobody expression using the Taguchi method
A. Farasat (2017)
10.1007/BF01569677
Production ofStreptomyces clavuligerus isopenicillin N synthase inEscherichia coli using two-cistron expression systems
J. L. Doran (2005)
10.1016/0734-9750(95)00004-A
Metabolic load and heterologous gene expression.
B. Glick (1995)
10.1007/BF00128622
Effects of inducer levels on a recombinant bacterial biofilm formation and gene expression
Ching-Tsan Huang (2004)
10.1016/j.pep.2013.02.007
Bacterial expression and antibiotic activities of recombinant variants of human β-defensins on pathogenic bacteria and M. tuberculosis.
L. Corrales-Garcia (2013)
Development of antibody-based strategies for the detection of mastitis and its causes in dairy cattle
S. Arora (2011)
10.1128/aem.58.12.4038-4041.1992
High-level expression of the Streptomyces clavuligerus isopenicillin N synthase gene in Escherichia coli.
M. Durairaj (1992)
10.1016/J.GENE.2005.06.042
A new vector for controllable expression of an anti-HER2/neu mini-antibody-barnase fusion protein in HEK 293T cells.
E. M. Glinka (2006)
Development of a high throughput cell-free metagenomic screening platform
Walter Nevondo (2016)
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