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The Mitochondrial Genome Of A Cytoplasmic Male Sterile Line Of Perennial Ryegrass (Lolium Perenne L.) Contains An Integrated Linear Plasmid-like Element

P. McDermott, V. Connolly, T. Kavanagh
Published 2008 · Biology, Medicine

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The mitochondrial genome of a cytoplasmic male sterile line of perennial ryegrass (Lolium perenne L.) was shown to contain a 9.6 kb element, LpCMSi, that is absent in the mitochondrial genome of fertile lines. LpCMSi contains the previously described chimeric gene orfC9, and three additional open reading frames (orfs) encoding a unique 45 kDa predicted protein of unknown function, a family B-like DNA polymerase (LpDpo), and a phage-type single subunit RNA polymerase (LpRpo). The latter two proteins shared significant similarity with DNA and RNA polymerases encoded by extrachromosomal linear mitochondrial plasmids of plants and fungi, and also to integrated plasmid-like sequences found in various plant and fungal mitochondrial genomes. Transcripts for both LpDpo and LpRpo were detected by RT-PCR in mitochondria of the CMS line. PCR-based investigations further revealed the presence of LpCMSi-like sequences in fertile L. perenne lines that are likely maintained as low-copy number extrachromosomal replicons. The absence of integrated forms of LpCMSi in the mitochondrial genome of fertile lines suggests that LpCMSi integration adjacent to the atp9 gene may be responsible, directly or indirectly, for the sterility phenotype of the CMS line.
This paper references
10.1093/nar/28.13.2571
The complete nucleotide sequence of the mitochondrial genome of sugar beet (Beta vulgaris L.) reveals a novel gene for tRNACys(GCA)
T. Kubo (2000)
10.1007/s00438-005-1133-x
Identification and distribution of sequences having similarity to mitochondrial plasmids in mitochondrial genomes of filamentous fungi
Patrick Cahan (2005)
10.1093/NAR/GKG795
The complete nucleotide sequence and RNA editing content of the mitochondrial genome of rapeseed (Brassica napus L.): comparative analysis of the mitochondrial genomes of rapeseed and Arabidopsis thaliana.
H. Handa (2003)
10.1007/BF00391475
A linear 10.4 kb plasmid in the mitochondria of Beta maritima
P. Saumitou-Laprade (2004)
10.1007/BF00352440
Genetic organization and structural features of maranhar, a senescence-inducing linear mitochondrial plasmid of Neurospora crassa
D. Court (2004)
10.1007/s00438-004-1075-8
The complete nucleotide sequence and multipartite organization of the tobacco mitochondrial genome: comparative analysis of mitochondrial genomes in higher plants
Y. Sugiyama (2004)
Cytoplasmic male sterility (CMS) in Lolium perenne L.2. The mitochondrial genome of a CMS line
AS Kiang (1996)
10.1104/pp.104.044602
Sequence and Comparative Analysis of the Maize NB Mitochondrial Genome1[w]
S. Clifton (2004)
10.1002/j.1460-2075.1987.tb04832.x
Stoichiometric differences in DNA molecules containing the atpA gene suggest mechanisms for the generation of mitochondrial genome diversity in maize
I. Small (1987)
Mitochondrial plasmids: DNA and RNA. In: Levings CS III, Vasil IK (eds) The molecular biology of plant mitochondria
GG Brown (1995)
10.1093/NAR/16.9.4169
Sequence of integrated S-1 homologous DNA in the normal maize mitochondrial genome.
J. A. García (1988)
A mutalistic fungal symbiont of perennial ryegrass contains two di V erent pyr 4
MA Collett (1995)
10.1186/1471-2148-7-135
Extensive variation in synonymous substitution rates in mitochondrial genes of seed plants
Jeffrey P. Mower (2007)
10.1007/s00294-004-0549-x
A mitochondrial plasmid and plasmid-like RNA and DNA polymerases encoded within the mitochondrial genome of carrot (Daucus carota L.)
M. Robison (2004)
10.1016/0147-619X(85)90011-3
Cytoplasmic reversion to fertility in cms-S maize need not involve loss of linear mitochondrial plasmids.
L. J. Escote (1985)
CLUSTALW: improving the sensitivity of progressive multiple sequence alignment
JD Thompson (1994)
10.1073/PNAS.95.24.14003
Mass migration of a group I intron: promiscuity on a grand scale.
M. Gray (1998)
10.1007/s00438-004-1058-9
The cytoplasmic male-sterile type and normal type mitochondrial genomes of sugar beet share the same complement of genes of known function but differ in the content of expressed ORFs
M. Satoh (2004)
10.1007/BF00222893
Paternal inheritance of mitochondria and chloroplasts in Festuca pratensis-Lolium perenne intergeneric hybrids
A. Kiang (2004)
10.1093/BIOINFORMATICS/17.12.1244
MEGA2: molecular evolutionary genetics analysis software
S. Kumar (2001)
10.1007/s00438-002-0711-4
Structural features and expression analysis of a linear mitochondrial plasmid in rapeseed (Brassica napus L.)
H. Handa (2002)
Gapped BLAST and PSI-BLAST: A new
D. Lipman (1997)
10.1007/978-94-011-0163-9_2
Mitochondrial Plasmids: DNA and RNA
G. G. Brown (1995)
10.1007/BF00329647
Molecular cloning and physical characterization of a Brassica linear mitochondrial plasmid
T. Turpen (2004)
10.1006/FGBI.1998.1105
Widespread distribution of low-copy-number variants of mitochondrial plasmid pEM in the genus Agaricus.
M. Robison (1999)
10.1111/j.1365-2958.1993.tb00897.x
The phage RNA polymerases are related to DNA polymerases and reverse transcriptases
W. McAllister (1993)
10.1016/0022-2836(92)90708-R
Gene organization deduced from the complete sequence of liverwort Marchantia polymorpha mitochondrial DNA. A primitive form of plant mitochondrial genome.
K. Oda (1992)
10.1007/BF02428050
TheBrassica mitochondrial DNA plasmid and large RNAs are not exclusively associated with cytoplasmic male sterility
R. Kemble (2006)
Cytoplasmic male sterility (CMS) in Lolium perenne L
AS Kiang (1993)
10.1146/ANNUREV.ARPLANT.55.031903.141735
Symbioses of grasses with seedborne fungal endophytes.
C. Schardl (2004)
10.1093/NAR/22.22.4673
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
J. Thompson (1994)
10.1073/PNAS.0408302101
Mitochondrial substitution rates are extraordinarily elevated and variable in a genus of flowering plants.
Y. Cho (2004)
10.1007/s00438-002-0767-1
The complete sequence of the rice (Oryza sativa L.) mitochondrial genome: frequent DNA sequence acquisition and loss during the evolution of flowering plants
Y. Notsu (2002)
Cytoplasmic reversion to fertility in cms - S need not involve loss of linear mitochondrial
JA Garcia (1988)
10.1007/BF00326237
The kalilo linear senescence-inducing plasmid of Neurospora is an invertron and encodes DNA and RNA polymerases
Benedict Shiu-Shing Chan (2004)
10.1016/0092-8674(89)90883-0
A conserved 3′→5′ exonuclease active site in prokaryotic and eukaryotic DNA polymerases
A. Bernad (1989)
10.1002/j.1460-2075.1987.tb02770.x
Structural and functional relationships between prokaryotic and eukaryotic DNA polymerases.
A. Bernad (1987)
10.1002/j.1460-2075.1985.tb03749.x
Nucleotide sequence of the S‐1 mitochondrial DNA from the S cytoplasm of maize
M. Paillard (1985)
10.1016/0092-8674(89)90890-8
Molecular biology of plant mitochondria
C. Levings (1989)
10.1007/BF00712969
A DNA-polymerase-related reading frame (pol-r) in the mtDNA ofSecale cereale
G. Dohmen (2004)
10.1038/301725A0
An unusual mitochondrial DNA plasmid in the genus Brassica
J. Palmer (1983)
10.1126/SCIENCE.277.5327.809
Mitochondrial and chloroplast phage-type RNA polymerases in Arabidopsis.
B. Hedtke (1997)
10.1073/pnas.1037651100
Substoichiometric shifting in the plant mitochondrial genome is influenced by a gene homologous to MutS
R. V. Abdelnoor (2003)
10.1007/BF00330952
The linear mitochondrial plasmid pClK1 of the phytopathogenic fungus Claviceps purpurea may code for a DNA polymerase and an RNA polymerase
B. Oeser (2004)
10.1073/PNAS.95.24.14244
Explosive invasion of plant mitochondria by a group I intron.
Y. Cho (1998)
Natural plasmids in W lamentous fungi
H Handa (2002)
10.1016/0378-1119(95)00143-T
A mutualistic fungal symbiont of perennial ryegrass contains two different pyr4 genes, both expressing orotidine-5'-monophosphate decarboxylase.
M. Collett (1995)
10.1007/BF00311221
Remnants of a DNA polymerase gene in the mitochondrial DNA of Marchantia polymorpha
B. Weber (2004)
10.1128/MMBR.59.4.673-685.1995
Natural plasmids of filamentous fungi.
A. J. Griffiths (1995)
10.1007/BF00175814
Fungal origin by horizontal transfer of a plant mitochondrial group I intron in the chimeric coxI gene of Peperomia
J. Vaughn (2004)
10.1007/BF00223673
Cytoplasmic male sterility (CMS) in Lolium perenne L. 2. The mitochondrial genome of a CMS line is rearranged and contains a chimaeric atp 9 gene
A. Kiang (2004)
10.1007/BF00312851
Linear plasmids among eukaryotes: fundamentals and application
F. Meinhardt (2004)
MEGA: Molecular Evolutionary Genetics Analysis
S. Kumar (1993)
10.1093/nar/gki925
Structural dynamics of cereal mitochondrial genomes as revealed by complete nucleotide sequencing of the wheat mitochondrial genome
Y. Ogihara (2005)
10.1007/BF00254817
Induction of cytoplasmic male-sterility into ryegrass (Lolium perenne)
V. Connolly (2004)
10.1073/PNAS.80.13.4055
Nucleotide sequence of the S-2 mitochondrial DNA from the S cytoplasm of maize.
C. Levings (1983)
10.1146/ANNUREV.BI.60.070191.000351
Protein-priming of DNA replication.
M. Salas (1991)
10.1016/0092-8674(85)90041-8
Mitochondrial DNA rearrangements associated with fertile revertants of S-type male-sterile maize
C. Schardl (1985)
Origin of a fungal symbiont of perennial ryegrass by interspecific hybridization of a mutualist with the ryegrass choke pathogen, Epichloë typhina.
C. Schardl (1994)
10.1038/NG0197-57
The mitochondrial genome of Arabidopsis thaliana contains 57 genes in 366,924 nucleotides
M. Unseld (1997)
10.1007/BF00222670
Cytoplasmic male sterility (CMS) in Lolium perenne L.: 1. Development of a diagnostic probe for the male-sterile cytoplasm
A. Kiang (2004)
10.1073/PNAS.84.23.8287
Bacteriophage PRD1 DNA polymerase: evolution of DNA polymerases.
G. Jung (1987)



This paper is referenced by
10.1093/jxb/erp361
Heteroplasmy and stoichiometric complexity of plant mitochondrial genomes--though this be madness, yet there's method in't.
M. Woloszynska (2010)
10.1155/2010/620137
Cost of Having the Largest Mitochondrial Genome: Evolutionary Mechanism of Plant Mitochondrial Genome
Kazuyoshi Kitazaki (2010)
10.1016/j.biochi.2013.09.016
The plant mitochondrial genome: dynamics and maintenance.
J. Gualberto (2014)
10.3389/fpls.2015.00764
Design of an F1 hybrid breeding strategy for ryegrasses based on selection of self-incompatibility locus-specific alleles
L. Pembleton (2015)
Organelle Genetics: Evolution of Organelle Genomes and Gene Expression. 2011. Bullerwell C.E. (Ed.). Springer Verlag.
Georg Hausner (2011)
Hybrid breeding in perennial grasses based on self- incompatibility and self-fertility
A. A. Aguirre (2013)
10.1101/610543
Genotyping-by-sequencing based cytoplasmic markers underpin population structural variation of perennial ryegrass
M. Cao (2019)
10.3389/fpls.2013.00128
Mitochondrial Genome Sequence of the Legume Vicia faba
V. Negruk (2013)
10.4238/2013.October.30.5
Analysis of mitochondrial DNA using amplified fragment length polymorphism markers of isonuclear allocytoplasmic male sterile wheat accessions and their maintainer lines.
M Ejaz (2013)
10.1371/journal.pone.0046747
The Mitochondrial Genome of an Aquatic Plant, Spirodela polyrhiza
W. Wang (2012)
10.1007/978-3-7091-1130-7_9
Plant Mitochondrial Genome Diversity: The Genomics Revolution
Jeffrey P. Mower (2012)
Peptides de pénétration cellulaire
Angelita Rebollo García (2012)
10.1371/journal.pone.0016404
The Mitochondrial Genome of the Legume Vigna radiata and the Analysis of Recombination across Short Mitochondrial Repeats
Andrew J. Alverson (2011)
Mécanisme et origine de l’édition des ARN messagers des mitochondries de plante
B. Castandet (2010)
10.1080/07352689.2011.587727
Male Sterility-Inducing Mitochondrial Genomes: How Do They Differ?
T. Kubo (2011)
10.1101/564278
The alternative reality of plant mitochondrial DNA
Alexander Kozik (2019)
10.1093/gbe/evw003
Linear Plasmids and the Rate of Sequence Evolution in Plant Mitochondrial Genomes
J. Warren (2016)
10.1111/PBR.12155
Genetics and biology of cytoplasmic male sterility and its applications in forage and turf grass breeding
Md. Shofiqul Islam (2014)
10.1093/gbe/evw047
In Silico Identification of Candidate Genes for Fertility Restoration in Cytoplasmic Male Sterile Perennial Ryegrass (Lolium perenne L.)
T. Sykes (2017)
10.1139/g09-006
A one-megabase physical map provides insights on gene organization in the enormous mitochondrial genome of cucumber.
G. Bartoszewski (2009)
10.3390/cells8020111
Identification of Extrachromosomal Linear microDNAs Interacted with microRNAs in the Cell Nuclei
Teng Fei Sun (2019)
10.1371/journal.pgen.1008373
The alternative reality of plant mitochondrial DNA: One ring does not rule them all
Alexander Kozik (2019)
10.1002/bies.201400052
Unique features of DNA replication in mitochondria: A functional and evolutionary perspective
I. Holt (2014)
10.1007/978-3-642-22380-8_13
Introns, Mobile Elements, and Plasmids
G. Hausner (2012)
10.1007/s12155-011-9166-y
Prospects for Hybrid Breeding in Bioenergy Grasses
Andrea Arias Aguirre (2011)
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