Online citations, reference lists, and bibliographies.
← Back to Search

Evaluation Of Inter-simple Sequence Repeat Analysis For Mapping In Citrus And Extension Of The Genetic Linkage Map

A. Sankar, G. Moore
Published 2001 · Biology

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
Abstract Inter-simple sequence repeat (ISSR) analysis was evaluated for its usefulness in generating markers to extend the genetic linkage map of Citrus using a backcross population previously mapped with restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD) and isozyme markers. ISSR markers were obtained through the simple technique of PCR followed by analysis on agarose gels, using simple sequence repeat (SSR) primers. Optimization of reaction conditions was achieved for 50% of the SSR primers screened, and the primers amplified reproducible polymorphic bands in the parents and progeny of the backcross population. Mendelian segregation of the polymorphic bands was demonstrated, with an insignificant number of skewed loci. Most of the SSR primers produced dominant loci; however co-dominance was observed with loci derived from three primers. A new genetic map was produced by combining the segregation data for the ISSR markers and data for the RFLP, RAPD and isozyme markers from the previous map and creating genetic linkages among all the markers using JoinMap 2.0 mapping software. The new map has an improved distribution of markers along the linkage groups with fewer gaps, and marker order showed partial or complete conservation in the linkage groups. The incorporation of ISSR markers into the genetic linkage map demonstrates that ISSR markers are suitable for genetic mapping in Citrus.
This paper references
Chromosome studies in Citrus . I
AK Sharma (1957)
A hypervariable microsatellite revealed by in vitro amplification of a dinucleotide repeat within the cardiac muscle actin gene.
M. Litt (1989)
A synergistic gelling and sieving agent for gel electrophoresis in normal and low melting temperature agarose.
D. Perlman (1991)
10.1046/J.1365-313X.1993.T01-9-00999.X
PCR-amplified microsatellites as markers in plant genetics.
M. Morgante (1993)
10.1111/J.1365-313X.1993.00739.X
Construction of integrated genetic linkage maps by means of a new computer package: JOINMAP.
P. Stam (1993)
10.1038/hdy.1993.131
Cytogenetics of Rutaceae. V. High chromosomal variability in Citrus species revealed by CMA/DAPI staining
M. Guerra (1993)
10.1093/NAR/22.15.3257
Detection of microsatellite polymorphisms without cloning.
K. S. Wu (1994)
10.1006/GENO.1994.1151
Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification.
E. Zietkiewicz (1994)
DrawMap: A computer program for drawing genetic linkage maps
J. V. Ooijen (1994)
Ampli - fication of DNA markers from evolutionarily diverse genomes using single primers of simple sequence repeats
M Gupta (1994)
Obtaining genetic markers by using double-stringency PCR with microsatellites and arbitrary primers.
S. R. Matioli (1995)
Simple and quick procedure for preparing citrus genomic DNA for PCR analysis
RE Durham (1996)
repertoires at mature stage of citrus fruits through random sequencing and analysis of redundant metallothionein - like genes expressed during fruit development
RG Novy (1996)
10.1007/s001220050468
Integration of trinucleotide microsatellites into a linkage map of Citrus
J. M. Kijas (1997)
10.1007/s001220050550
Fingerprinting trifoliate orange germ plasm accessions with isozymes, RFLPs, and inter-simple sequence repeat markers
D. Fang (1997)
10.1016/S0378-1119(98)00138-3
Characterization of gene repertoires at mature stage of citrus fruits through random sequencing and analysis of redundant metallothionein-like genes expressed during fruit development.
T. Moriguchi (1998)



This paper is referenced by
10.1007/978-3-030-47829-2_5
Genetic Diversity Analysis of Mango
Xinhua He (2021)
10.1007/978-3-030-15308-3_7
Markers, Maps, and Marker-Assisted Selection
Tokurou Shimizu (2020)
10.1007/s10341-020-00508-8
Identification of Citruses from Montenegro Based on Microsatellite Clustering Analyses
Lidija Bitz (2020)
10.1186/s43141-020-00069-z
A systematic revision of Capparaceae and Cleomaceae in Egypt: an evaluation of the generic delimitations of Capparis and Cleome using ecological and genetic diversity
M. A. E. El Zayat (2020)
10.3906/tar-1909-67
Identification of genetic diversity among mutant lemon and mandarin varieties using different molecular markers
G. Sülü (2020)
10.1007/978-3-319-97946-5_5
Toward Development of Climate-Resilient Citrus
Supratim Basu (2020)
10.21608/taec.2019.19054.1007
Genetic variation within and among the wild populations of Alhagi graecorum using ISSR markers
ghada Abd El- hak (2019)
Studies on the constraints in efficient micropropagation of bamboo
V. Sankar (2019)
10.1007/s12298-019-00723-y
Genetic diversity and population structure detection in sponge gourd (Luffa cylindrica) using ISSR, SCoT and morphological markers
R. Tyagi (2019)
10.31421/IJHS/25/1-2./2912
Mutation induction in sweet basil (Ocimum basilicum L.) by fast neutron irradiation
E. Enkhbileg (2019)
10.30848/PJB2019-5(41)
Genetic diversity of Symplocos paniculata of Hunan province revealed by inter-simple sequence repeat (ISSR)
Cao Huifang (2019)
10.1007/s11033-019-05173-z
Genetic structure and diversity analysis of tall fescue populations by EST-SSR and ISSR markers
Z. Shahabzadeh (2019)
10.21608/assjm.2018.65123
Salinity Stress Responses in Some Grape Rootstocks.
H. A. Aziz (2018)
10.3390/plants7020046
Characterization of the Genetic Diversity of Acid Lime (Citrus aurantifolia (Christm.) Swingle) Cultivars of Eastern Nepal Using Inter-Simple Sequence Repeat Markers
N. N. Munankarmi (2018)
10.21608/EJGC.2018.9212
BIODIVERSITY ASSESSMENT FOR SOME ALMOND GENOTYPES CULTIVATED IN LIBYA USING SRAP AND ISSR
A. Abo-Doma (2018)
10.1007/s13580-018-0021-3
Construction of genetic linkage maps of ‘Fina Sodea’ clementine (Citrus clementina) and Byungkyul (C. platymamma), a Korean landrace, based on RAPD and SSR markers
Yali Chang (2018)
10.20944/PREPRINTS201805.0144.V1
ISSR-Markers Assisted Genetic Diversity Assessment of Acid Lime [Citrus aurantifolia (Christm.) Swingle] Germplasm of Eastern Nepal
N. N. Munankarmi (2018)
10.21608/jpp.2018.36400
Evaluation of a New Thornless Strain of Mexican Lime (Citrus aurantifolia, Swingle) Under Kaferelsheikh Governorate Conditions, Egypt
M. A. El-Enien (2018)
10.1111/jam.13582
Genetic diversity and pathogenicity of Fusarium species associated with fruit rot disease in banana across Peninsular Malaysia
N. B. Abd Murad (2017)
10.17660/ACTAHORTIC.2017.1185.25
Assessment of the genetic diversity and phylogenetic relationship of Dianthus caryophyllus germplasm using ISSR and RAPD molecular markers
Abinaya Manivannan (2017)
10.21475/poj.10.05.17.pne794
Utilization of ISTR, ISSR and SRAP molecular markers to reveal and classify Egyptian pomegranates ('Punica granatum' L.)
M. Amar (2017)
10.21608/sinjas.2017.78829
ASSESSMENT OF GENETIC DIVERSITY OF SOME CANOLA GENOTYPES
Aya Abdelsalam (2017)
10.1007/978-1-4939-7159-6_10
Genetic Diversity Analysis of Date Palm Using Random Amplified Polymorphic DNA (RAPD) and Inter-Simple Sequence Repeat (ISSR).
M. Mahatma (2017)
10.18782/2320-7051.2480
Study of Genetic Variability in Citrus Fruit Crop by Molecular Markers - A Review
Shahnawaz Ahmed (2017)
10.1007/978-981-10-4732-9_12
Recent Updates on Molecular Biotechnological Intervention in Isabgol
S. Jaglan (2017)
MOLECULAR FINGERPRINTING AND MARKER-ASSISTED SELECTION IN CITRUS SPECIES
F. Abdel-Tawab (2016)
10.2298/GENSR1601307K
Molecular analysis of commercial date palm cultivars in Lybia using ISSR and SRAP PCR-based markers
Noha S Khalifa (2016)
10.4238/gmr.15026564
Assessment of genetic diversity of Tunisian orange, Citrus sinensis (L.) Osbeck using microsatellite (SSR) markers.
A. Mahjbi (2016)
MOLECULAR ANALYSIS OF COMMERCIAL DATE PALM CULTIVARS IN LYBIA USING ISSR AND SRAP PCR-BASED MARKERS
Umfetity (2016)
IDENTIFICATION OF MOLECULAR MARKERS LINKED TO YELLOW MOSAIC VIRUS RESISTANCE IN BLACKGRAM (Vigna mungo (L.) Hepper)
E. Rambabu (2016)
10.1007/s11295-016-1034-7
QTL mapping of mandarin (Citrus reticulata) fruit characters using high-throughput SNP markers
Yuan Yu (2016)
10.1007/s12298-016-0336-4
Genetic variation assessment of acid lime accessions collected from south of Iran using SSR and ISSR molecular markers
A. Sharafi (2016)
See more
Semantic Scholar Logo Some data provided by SemanticScholar