Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

A Nanoflow Cytometric Strategy For Sensitive CtDNA Detection Via Magnetic Separation And DNA Self-assembly

Xingwei Zhai, J. Li, Y. Cao, X. Zhu, Yuguo Tang, G. Chen, Kun Han
Published 2019 · Chemistry, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
AbstractCirculating tumor DNA (ctDNA) is a tumor-derived fragmented DNA in the bloodstream that is not associated with cells. It has been greatly focused in the recent decade because of its potential clinical utility for liquid biopsies. Development of ctDNA analytical techniques with high sensitivity and cost-efficiency will undoubtedly promote the clinical spread of ctDNA testing. In this paper, we propose a novel flow cytometry–based ctDNA sensing strategy which combines enzyme-free amplification and magnetic separation. The target DNA is capable of triggering a hybridization chain reaction, producing a fluorescent long linear assembly of DNA, which can be further captured by magnetic beads to present fluorescent signals using flow cytometry. In comparison with some conventional methods, our strategy has the advantages of easy operation and cost-efficiency, and thereby shows a promising application in clinical diagnosis. Graphical abstract
This paper references
Highly selective detection of single-nucleotide polymorphisms using a quartz crystal microbalance biosensor based on the toehold-mediated strand displacement reaction.
Dingzhong Wang (2012)
A flow cytometry assay to quantify intercellular exchange of membrane components† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc00260b Click here for additional data file.
Dimitrios Poulcharidis (2017)
Circulating Tumor DNA as Biomarkers for Cancer Detection
Xiao Han (2017)
Control of DNA strand displacement kinetics using toehold exchange.
D. Y. Zhang (2009)
High sensitivity automated multiplexed immunoassays using photonic crystal enhanced fluorescence microfluidic system.
Yafang Tan (2015)
DNA Clutch Probes for Circulating Tumor DNA Analysis.
Jagotamoy Das (2016)
Hybridization chain reaction-based flow cytometric bead sensor for the detection of emetic Bacillus cereus in milk
Bei Yu (2018)
An exonuclease IIIpowered, on-particle stochastic DNAwalker
QuX (2016)
Programming Chemical Reaction Networks Using Intramolecular Conformational Motions of DNA.
Wei Lai (2018)
Enzyme-Free Nucleic Acid Logic Circuits
Georg Seelig (2006)
Hybridization chain reaction: a versatile molecular tool for biosensing, bioimaging, and biomedicine.
S. Bi (2017)
DNA Origami-Graphene Hybrid Nanopore for DNA Detection.
A. Barati Farimani (2017)
Identification and Characterization of Mitochondrial Subtypes in Caenorhabditis elegans via Analysis of Individual Mitochondria by Flow Cytometry.
J. Daniele (2016)
Highly Specific Binding on Antifouling Zwitterionic Polymer-Coated Microbeads as Measured by Flow Cytometry
E. van Andel (2017)
An Exonuclease III-Powered, On-Particle Stochastic DNA Walker.
Xiangmeng Qu (2017)
Triggered amplification by hybridization chain reaction.
Robert M. Dirks (2004)
Circulating RNAs as new biomarkers for detecting pancreatic cancer.
T. Kishikawa (2015)
A one-step and label-free, electrochemical DNA detection using metal ion-mediated molecular beacon probe
Songyi Baek (2019)
Stimuli-Triggered Strand Displacement-Based Multifunctional Gene Detection Platform Controlled By A Multi-Input DNA Logic Gate
Shuang Wu (2018)
Simple, Sensitive and Accurate Multiplex Detection of Clinically Important Melanoma DNA Mutations in Circulating Tumour DNA with SERS Nanotags
E. Wee (2016)
Sensitive Detection of Staphylococcus aureus with Vancomycin-Conjugated Magnetic Beads as Enrichment Carriers Combined with Flow Cytometry.
Xiangyu Meng (2017)
Capillary Electrophoresis with Laser-Induced Fluorescent Detection of Immunolabeled Individual Autophagy Organelles Isolated from Liver Tissue.
K. A. Muratore (2016)
A DNA-fuelled molecular machine made of DNA
B. Yurke (2000)
In situ characterization of nanoparticle biomolecular interactions in complex biological media by flow cytometry
M. L. Lo Giudice (2016)
An ultrasensitive flow cytometric immunoassay based on bead surface-initiated template-free DNA extension† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c8sc02752h
L. Zhu (2018)
Protected DNA strand displacement for enhanced single nucleotide discrimination in double-stranded DNA
D. Khodakov (2015)
Antibody-based assay discriminates Zika virus infection from other flaviviruses
A. Balmaseda (2017)
A cytometric assay for ultrasensitive and robust detection of human telomerase RNA based on toehold strand displacement.
Jing Xu (2017)
DNA-Encoded Raman-Active Anisotropic Nanoparticles for microRNA Detection.
L. Qi (2017)
Remote toehold: a mechanism for flexible control of DNA hybridization kinetics.
A. J. Genot (2011)
Programming Cell Adhesion for On-Chip Sequential Boolean Logic Functions.
Xiangmeng Qu (2017)
Methodology for Y Chromosome Capture: A complete genome sequence of Y chromosome using flow cytometry, laser microdissection and magnetic streptavidin-beads
M. J. Alvarez-Cubero (2018)
Challenges in Using Circulating miRNAs as Cancer Biomarkers
P. Tiberio (2015)
Pitfalls in diagnosis with the use of circulating tumor‐derived epidermal growth factor receptor mutations in lung cancer harboring pretreatment T790M
D. Ogawara (2018)
Novel Surface-Enhanced Raman Spectroscopy Techniques for DNA, Protein and Drug Detection
C. Chen (2019)
Biosensors for liquid biopsy: circulating nucleic acids to diagnose and treat cancer
Noemi Bellassai (2016)
Nucleic acid detection with CRISPR-Cas13a/C2c2
J. Gootenberg (2017)

This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar