Online citations, reference lists, and bibliographies.
← Back to Search

The Use Of Phospholipid Fatty Acid Analysis To Measure Impact Of Acid Rock Drainage On Microbial Communities In Sediments

Eric Ariel Ben-David, P. Holden, D. Stone, B. Harch, L. Foster
Published 2003 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Share
The impact of acid rock drainage (ARD) and eutrophication on microbial communities in stream sediments above and below an abandoned mine site in the Adelaide Hills, South Australia, was quantified by PLFA analysis. Multivariate analysis of water quality parameters, including anions, soluble heavy metals, pH, and conductivity, as well as total extractable metal concentrations in sediments, produced clustering of sample sites into three distinct groups. These groups corresponded with levels of nutrient enrichment and/or concentration of pollutants associated with ARD. Total PLFA concentration, which is indicative of microbial biomass, was reduced by >70% at sites along the stream between the mine site and as far as 18 km downstream. Further downstream, however, recovery of the microbial abundance was apparent, possibly reflecting dilution effect by downstream tributaries. Total PLFA was >40% higher at, and immediately below, the mine site (0–0.1 km), compared with sites further downstream (2.5–18 km), even after accounting for differences in specific surface area of different sediment samples. The increased microbial population in the proximity of the mine source may be associated with the presence of a thriving iron-oxidizing bacteria community as a consequence of optimal conditions for these organisms while the lower microbial population further downstream corresponded with greater sediments’ metal concentrations. PCA of relative abundance revealed a number of PLFAs which were most influential in discriminating between ARD-polluted sites and the rest of the sites. These PLFA included the hydroxy fatty acids: 2OH12:0, 3OH12:0, 2OH16:0; the fungal marker: 18:2ω6; the sulfate-reducing bacteria marker 10Me16:1ω7; and the saturated fatty acids 12:0, 16:0, 18:0. Partial constrained ordination revealed that the environmental parameters with the greatest bearing on the PLFA profiles included pH, soluble aluminum, total extractable iron, and zinc. The study demonstrated the successful application of PLFA analysis to rapidly assess the toxicity of ARD-affected waters and sediments and to differentiate this response from the effects of other pollutants, such as increased nutrients and salinity.
This paper references
10.1016/S0167-7012(01)00245-7
Comparison of microbial and meiofaunal community analyses for determining impact of heavy metal contamination.
R. Ellis (2001)
10.1128/AEM.63.4.1476-1482.1997
Variation in microbial community structure in two boreal peatlands as determined by analysis of phospholipid Fatty Acid profiles.
I. Sundh (1997)
10.1021/BK-1997-0671.CH004
Seasonal Variation in Sedimentary Microbial Community Structure as a Backdrop for the Detection of Anthropogenic Stress
R. Findlay (1997)
Acid Mine Drainage in Australia
J Harries (1997)
10.1016/S0580-9517(08)70410-0
4 Lipid and Cell-Wall Analysis in Bacterial Systematics
K. Komagata (1988)
Microbial Lipids, Vol. 1
C Ratledge (1988)
Biogeochemistry of acid mine drainage and a method to control acid formation
R.L.P. Kleinmann (1981)
10.1007/978-94-009-0215-2_7
The use of phospholipid fatty acids to determine microbial community structure
R. Findlay (1996)
10.1007/978-1-4899-3564-9_16
Use of Lipid Biomarkers in Environmental Samples
A. Tunlid (1990)
10.3354/MEPS133149
Microbial biomass and community structures in the burrows of bromophenol producing and non-producing marine worms and surrounding sediments
Steward Cc (1996)
Fatty acid and menaquinone analysis of actinomycetes and related organisms. In: Goodfellow, M, Minni- kin, DE (Eds.) Chemical Methods in Bacterial Systematics
RM Kroppenstedt (1985)
10.2307/1310976
Lipid analysis in microbial ecology: quantitative approaches to the study of microbial communities.
J. Vestal (1989)
Extractable and lipopolysaccharide fatty acid and hydroxy acid profiles from Desulfovibrio species.
A. Edlund (1985)
10.1007/978-94-009-1359-2
Mining and the Freshwater Environment
Martyn Kelly (1988)
10.1016/0025-3227(85)90013-1
Biomass and community structure of the abyssal microbiota determined from the ester-linked phospholipids recovered from Venezuela Basin and Puerto Rico Trench sediments.
B. H. Baird (1985)
Primer User Manual—Plymouth Routines in Multivariate Ecological Research
MR Carr (1996)
10.1007/978-3-662-06111-4_13
Heterotrophic Acidophiles and Their Roles in the Bioleaching of Sulfide Minerals
D. Johnson (1997)
10.1016/0167-7012(95)00012-A
Combined microbial community-level analyses for quality assurance of terrestrial subsurface cores
R. M. Lehman (1995)
10.3354/MEPS045069
Callianassa trilobata (Crustacea: Thalassinidea) influences abundance of meiofauna and biomass, composition, and physiologic state of microbial communities within its burrow
F. Dobbs (1988)
10.1111/J.1574-6968.1986.TB01954.X
Signature fatty acids in the polar lipids of acid-producing Thiobacillus spp.: Methoxy, cyclopropyl, alpha-hydroxy-cyclopropyl and branched and normal monoenoic fatty acids
B. Kerger (1986)
10.3109/10408417709102311
Lipids in Bacterial Taxonomy - A Taxonomist's View
Lechevalier Mp (1977)
10.1007/BF01029321
Reducing the dimensionality of compositional data sets
J. Aitchison (1984)
10.1016/S0038-0717(99)00059-0
Changes in soil microbial communities over time resulting from one time application of zinc: a laboratory microcosm study
J. Kelly (1999)
10.1007/978-94-009-1359-2_5
Uptake and Accumulation of Heavy Metals
Martyn Kelly (1988)
10.1016/0269-7491(92)90105-J
Microbiological and chemical characteristics of an acidic stream draining a disused copper mine.
K. Walton (1992)
10.1007/S002540050204
Mine-water chemistry: the good, the bad and the ugly
D. Banks (1997)
10.1099/13500872-145-4-767
Polyunsaturated fatty acids in marine bacteria--a dogma rewritten.
N. Russell (1999)
10.1007/978-3-662-06111-4_11
Mesophilic, Autotrophic Bioleaching Bacteria: Description, Physiology and Role
D. Rawlings (1997)
Microbial Lipids, Vol. 1
C Ratledge (1988)
10.1128/AEM.55.11.2888-2893.1989
Efficacy of phospholipid analysis in determining microbial biomass in sediments.
R. Findlay (1989)
Biomining : Theory, Microbes and Industrial Processes
D. Rawlings (2006)
10.1007/S100400050016
Microbiological and chemical characterization of hydrothermal fluids at Tortugas Mountain Geothermal Area, southern New Mexico, USA
D. Schulze-Makuch (2000)
10.3109/10408417709102311
Lipids in bacterial taxonomy - a taxonomist's view.
M. Lechevalier (1977)
Risk-based assessment method for impact on riverine ecosystems.’
JM Ferris (2000)
The hydrolysis of metal ions. VIII: Aluminium(III)
P. Brown (1985)
10.1016/0016-7037(80)90067-8
Microbial lipids of an intertidal sediment—I. Fatty acids and hydrocarbons
J. Volkman (1980)
10.1007/978-1-4020-2177-0
Molecular Microbial Ecology Manual
A. Akkermans (2004)
10.1021/BK-1997-0671
Molecular markers in environmental geochemistry
R. Eganhouse (1997)
10.1016/0167-7012(84)90023-X
A method for the estimation of bacterial biomass and community structure in mangrove-associated sediments
F. T. Gillan (1984)
10.1128/AEM.64.1.238-245.1998
Effect of Metal-Rich Sludge Amendments on the Soil Microbial Community
E. Bååth (1998)
10.1128/AEM.59.11.3605-3617.1993
Phospholipid Fatty Acid composition, biomass, and activity of microbial communities from two soil types experimentally exposed to different heavy metals.
A. Frostegård (1993)
10.1128/MMBR.55.2.288-302.1991
Iso- and anteiso-fatty acids in bacteria: biosynthesis, function, and taxonomic significance.
T. Kaneda (1991)
Structure of a microbial community in soil after prolonged addition of low levels of simulated acid rain
T Pennanen (1998)
10.1039/DT9850001967
The hydrolysis of metal ions. Part 8. Aluminium(III)
P. Brown (1985)
10.1016/S0167-7012(97)00038-9
Comparison of substrate utilization assay and fatty acid analysis of soil microbial communities
J. Buyer (1997)
10.1007/978-1-4899-3564-9
Analytical Microbiology Methods
A. Fox (1990)
10.1007/BF00388810
Determination of the sedimentary microbial biomass by extractible lipid phosphate
D. White (2004)
10.1016/0167-7012(96)00929-3
The use of a classic lipid extraction method for simultaneous recovery of organic pollutants and microbial lipids from sediments
J. Fang (1996)
10.15027/24687
Fatty Acid Analysis to Determine the Seasonal Variation in Microbial Biomass and its Community Structure of Coastal Sediments
N. Rajendran (1995)
10.1007/BF00142333
An experimental manipulation of oligochaete communities in mesocosms treated with chlorpyrifos or nutrient additions: multivariate analyses with Monte Carlo permutation tests
P. Verdonschot (2004)
10.1111/J.1365-2672.1996.TB03266.X
Microbial biomass in a shallow, urban aquifer contaminated with aromatic hydrocarbons: analysis by phospholipid fatty acid content and composition.
P. D. Franzmann (1996)
Indicators of rehabilitation success in aquatic ecosystems
R Jeffree (1998)
10.1016/0038-0717(94)00140-V
MICROBIAL COMMUNITY STRUCTURE AND pH RESPONSE IN RELATION TO SOIL ORGANIC MATTER QUALITY IN WOOD-ASH FERTILIZED, CLEAR-CUT OR BURNED CONIFEROUS FOREST SOILS
E. Bååth (1995)
Water Resources and Problems Related to Mining
R Hadley (1974)
10.1520/STP695-EB
Native Aquatic Bacteria: Enumeration, Activity, and Ecology
Jw Costerton (1979)
Polyunsaturated fatty acids in marine bacteria—a dogma
JN Russell (1999)
10.1071/MF9960027
Microbial consortia in wetland sediments : A biomarker analysis of the effects of hydrological regime, vegetation and season on benthic microbes
P. I. Boon (1996)
10.1520/STP36005S
Biochemical Measurements of Microbial Mass and Activity from Environmental Samples
D. White (1979)
10.1111/J.1574-6941.2000.TB00735.X
Comparison of methods to investigate microbial populations in soils under different agricultural management
K. Lawlor (2000)
10.1007/BF02183058
Fatty acid methyl ester (FAME) profiles as measures of soil microbial community structure
M. Cavigelli (2004)
10.1016/s0021-9673(01)83552-4
Chemical methods in bacterial systematics
M. Goodfellow (1985)
10.1007/BF00411253
Effect of cultural conditions on the lipid profile of Thiobacillus ferroxidas
M. Ghosh (2004)
10.1099/00221287-132-7-1815
Phospholipid Ester-linked Fatty Acid Biomarkers of Acetate-oxidizing Sulphate-reducers and Other Sulphide-forming Bacteria
N. J. E. Dowling (1986)
10.1139/M86-022
Quantitative characterization of microbial biomass and community structure in subsurface material: a prokaryotic consortium responsive to organic contamination
G. A. Smith (1986)
10.1099/00221287-128-7-1599
Ubiquinone, Fatty acid and DNA Base Composition Determination as a Guide to the Taxonomy of the Genus Thiobacillus
Y. Katayama-Fujimura (1982)
A conceptual model of the chemical loads in Dawesley Creek arising from acid sulfates in the Brukunga quarry
I Dainis (2000)
Fatty acid and menaquinone analysis of actinomycetes and related organisms
R. Kroppenstedt (1985)
10.2307/1352289
Environmental impact of salmon net-pen culture on marine benthic communities in Maine: A case study
R. Findlay (1995)
10.1080/01490459209377918
Oxidation and Reduction of Iron by Acidophilic Bacteria
J. Pronk (1992)
10.1016/S0167-7012(98)00017-7
In situ microbial ecology for quantitative appraisal, monitoring, and risk assessment of pollution remediation in soils, the subsurface, the rhizosphere and in biofilms
D. White (1998)
10.1128/AEM.64.6.2173-2180.1998
Structure of a Microbial Community in Soil after Prolonged Addition of Low Levels of Simulated Acid Rain
T. Pennanen (1998)
10.1016/0964-8305(95)00065-D
Acidophilic microbial communities: Candidates for bioremediation of acidic mine effluents
D. Johnson (1995)
10.1128/AEM.49.1.179-186.1985
Sulfate reduction in freshwater sediments receiving Acid mine drainage.
A. T. Herlihy (1985)



This paper is referenced by
10.1016/S1002-0160(14)60041-2
Microbial Biomass and PLFA Profile Changes in Rhizosphere of Pakchoi (Brassica chinensis L.) as Affected by External Cadmium Loading
Jia-li Shentu (2014)
10.1007/s00374-007-0181-2
Experimentally induced effects of heavy metal on microbial activity and community structure of forest mor layers
Staffan Åkerblom (2007)
10.1139/W05-144
Physical and chemical factors affecting microbial biomass and activity in contaminated subsurface riverine sediments.
J. Mosher (2006)
10.1007/s00572-020-00962-y
Positive effects of co-inoculation with Rhizophagus irregularis and Serendipita indica on tomato growth under saline conditions, and their individual colonization estimated by signature lipids
M. B. Heidarianpour (2020)
10.7939/R32V2C92D
Community Level Physiological Profiling for Monitoring Oil Sands Impacts
J. Davies (2011)
Relationships of Benthic Macroinvertebrate Community Structure with Land-use, Habitat, In-stream Water Chemistry, Depositional Sediment Biofilm Fatty Acids, and Surfactants in the Effluent Dominated Texas Trinity River
Jaime L. Slye (2013)
10.1098/rstb.2019.0641
Stable isotopes of fatty acids: current and future perspectives for advancing trophic ecology
C. Twining (2020)
10.1016/J.CATENA.2018.02.001
The restoration age of Robinia pseudoacacia plantation impacts soil microbial biomass and microbial community structure in the Loess Plateau
Dong Liu (2018)
10.1007/S00254-008-1663-8
Influence of acid mine drainage on microbial communities in stream and groundwater samples at Guryong Mine, South Korea
J. Kim (2009)
10.3389/fpls.2019.01298
Fine-Root Turnover, Litterfall, and Soil Microbial Community of Three Mixed Coniferous–Deciduous Forests Dominated by Korean Pine (Pinus koraiensis) Along a Latitudinal Gradient
L. Liu (2019)
10.1007/s00792-007-0065-2
The isolation and initial characterization of mercury resistant chemolithotrophic thermophilic bacteria from mercury rich geothermal springs
A. Chatziefthimiou (2007)
, Approach Hildenborough : an Integrated Genomics Desulfovibrio vulgaris Salt Stress in
Aindrila Mukhopadhyay (2006)
10.3389/fpls.2015.00485
Context dependency and saturating effects of loss of rare soil microbes on plant productivity
W. H. G. Hol (2015)
10.3389/fmicb.2015.01507
(A)synchronous Availabilities of N and P Regulate the Activity and Structure of the Microbial Decomposer Community
Nicolas Fanin (2016)
10.3389/fmicb.2018.02060
Local Functioning, Landscape Structuring: Drivers of Soil Microbial Community Structure and Function in Peatlands
S. Teurlincx (2018)
10.3389/fpls.2017.02040
Effects of Plant Functional Group Loss on Soil Microbial Community and Litter Decomposition in a Steppe Vegetation
Chunwang Xiao (2017)
10.1111/j.1574-6968.2008.01178.x
Assessing the impact of the biological control agent Bacillus thuringiensis on the indigenous microbial community within the pepper plant phyllosphere.
Baoguo Zhang (2008)
10.3389/fmicb.2019.01488
Soil Microbial Biomass and Fungi Reduced With Canola Introduced Into Long-Term Monoculture Wheat Rotations
Jeremy C. Hansen (2019)
Heap Bioleaching of Low-grade Multimetal Sulphidic Ore in Boreal Conditions
Anna-Kaisa Halinen (2015)
The microbiology of oxalate degradation in bioreactors treating Bayer liquor organic wastes
N. J. McSweeney (2011)
Assessment of the biofumigation effect of Canola (Brassica napus) on soil microbial community function and structure
Clarissa. Potgieter (2012)
10.3389/fmicb.2014.00613
Investigations of potential microbial methanogenic and carbon monoxide utilization pathways in ultra-basic reducing springs associated with present-day continental serpentinization: the Tablelands, NL, CAN
P. L. Morrill (2014)
10.1089/ast.2018.1819
Recovery of Fatty Acids from Mineralogic Mars Analogs by TMAH Thermochemolysis for the Sample Analysis at Mars Wet Chemistry Experiment on the Curiosity Rover
A. J. Williams (2019)
10.1016/S1001-0742(08)62233-0
Effect of cypermethrin insecticide on the microbial community in cucumber phyllosphere.
Baoguo Zhang (2008)
10.1016/S1001-0742(08)62349-9
Biofilm structure and its influence on clogging in drip irrigation emitters distributing reclaimed wastewater.
D. Yan (2009)
10.1007/s11356-016-8278-5
Nutrient leaching, soil pH and changes in microbial community increase with time in lead-contaminated boreal forest soil at a shooting range area
S. Selonen (2016)
10.1890/08-0571.1
Variation in ecosystem function in Appalachian streams along an acidity gradient.
K. Simon (2009)
Autotrophy in Groundwater Ecosystems
Claudia Kellermann (2009)
10.1016/J.APSOIL.2017.01.009
Labile carbon and nitrogen additions affect soil organic matter decomposition more strongly than temperature
Qian-ru Li (2017)
10.1016/j.scitotenv.2008.11.049
The impacts of cypermethrin pesticide application on the non-target microbial community of the pepper plant phyllosphere.
Baoguo Zhang (2009)
10.1128/AEM.06029-11
Direct and Indirect Influence of Parental Bedrock on Streambed Microbial Community Structure in Forested Streams
J. Mosher (2011)
10.1016/J.SOILBIO.2010.02.002
Inferring biological soil crust successional stage using combined PLFA, DGGE, physical and biophysiological analyses
E. Zaady (2010)
See more
Semantic Scholar Logo Some data provided by SemanticScholar