← Back to Search
High-yield Growth And Magnetosome Formation By Magnetospirillum Gryphiswaldense MSR-1 In An Oxygen-controlled Fermentor Supplied Solely With Air
Jianbo Sun, F. Zhao, T. Tang, W. Jiang, Jiesheng Tian, Y. Li, J. Li
Published 2008 · Biology, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
Magnetotactic bacteria are difficult to grow under defined conditions in culture, which has presented a major obstacle to commercial application of magnetosomes. We studied the relationships among the cell growth, magnetosome formation, dissolved oxygen concentration (DO), and the ability to supply oxygen to the cells. Mass culture of Magnetospirillum gryphiswaldense MSR-1 for the production of magnetosomes was established in a 42-L fermentor under the following conditions: (1) sterile air was the sole gas supplied in the fermentor, and DO could be regulated at any level below 10% saturation by cascading the stir rate to DO, (2) to resolve the paradoxical situation that the cell growth requires higher DO whereas magnetosome formation requires low DO below the detectable range of regular oxygen electrode, DO was controlled to optimal level using the change of cell growth rate, rather than reading from the highly sensitive oxygen electrode, as the signal for determining appropriate DO, and (3) timing and rate of supplying the substrates were determined by measuring cell density and Na-lactate concentration. Under these conditions, cell density (OD565) of strain MSR-1 reached 7.24 after 60-h culture in a 42-L fermentor, and cell yield (dry weight) was 2.17 g/L, the highest yield so far being reported. The yield of magnetosomes (dry weight) was 41.7 mg/L and 16.7 mg/L/day, which were 2.8 and 2.7 times higher than the previously reported yields.
This paper references
Magneto immuno-PCR: a novel immunoassay based on biogenic magnetosome nanoparticles.
R. Wacker (2007)
JB Sun (2007)
Iron reductase for magnetite synthesis in the magnetotactic bacterium Magnetospirillum magnetotacticum.
Y. Noguchi (1999)
In vitro and in vivo antitumor effects of doxorubicin loaded with bacterial magnetosomes (DBMs) on H22 cells: the magnetic bio-nanoparticles as drug carriers.
J. Sun (2007)
Characterization of the bacterial magnetosome membrane.
Y. A. Gorby (1988)
Application of bacterial magnetic particles as novel DNA carriers for ballistic transformation of a marine cyanobacterium
H. Takeyama (1995)
Magnetosome formation in prokaryotes
D. Bazylinski (2004)
Ultrastructure of a magnetotactic spirillum.
D. Balkwill (1980)
SNP detection in transforming growth factor-beta1 gene using bacterial magnetic particles.
Hiroyuki Ota (2003)
Structure and function of the bacterial magneto - some
D Bazylinski (1995)
Highly sensitive detection of allergen using bacterial magnetic particles
N. Nakamura (1993)
Use of magnetic particles isolated from magnetotactic bacteria for enzyme immobilization
T. Matsunaga (2004)
The Magnetosomes of Magnetotactic Bacteria May be Used as Drug-carriers for Targeted Therapy
S. Jian (2007)
Electron microscopic studies of magnetosomes in magnetotactic bacteria
D. Bazylinski (1994)
Submerged culture of Magnetospirillum gryphiswaldense under N2-fixing condition and regulation of activity of nitrogen fixation
Wei Jiang (2002)
Fully automated immunoassay system of endocrine disrupting chemicals using monoclonal antibodies chemically conjugated to bacterial magnetic particles
T. Matsunaga (2003)
Isolation and pure culture of a freshwater magnetic spirillum in chemically defined medium.
R. Blakemore (1979)
Microaerobic Conditions Are Required for Magnetite Formation Within Aquaspirillum magnetotacticum
R. Blakemore (1985)
Bacterial magnetosomes: microbiology, biomineralization and biotechnological applications
D. Schüler (1999)
Effects of growth medium composition, iron sources and atmospheric oxygen concentrations on production of luciferase-bacterial magnetic particle complex by a recombinant Magnetospirillum magneticum AMB-1.
C. Yang (2001)
Diversity of Magnetotactic Bacteria
S. Spring (1995)
Rapid magnetosome formation shown by real-time x-ray magnetic circular dichroism
S. Staniland (2007)
Growth and magnetosome formation by microaerophilic Magnetospirillum strains in an oxygen-controlled fermentor
U. Heyen (2003)
Synthesis of magnetite nanoparticles for bio- and nanotechnology: genetic engineering and biomimetics of bacterial magnetosomes.
C. Lang (2007)
Enhancement of magnetic particle production by nitrate and succinate fed-batch culture of Magnetospirillum sp. AMB-1.
T. Matsunaga (1996)
Nanostructure and field-induced arrangement of magnetosomes studied by SANSPOL
A. Hoell (2004)
Dynamics of iron uptake and Fe3O4 biomineralization during aerobic and microaerobic growth of Magnetospirillum gryphiswaldense.
D. Schueler (1998)
Magnet-making bacteria could target tumours
M. Hopkin (2004)
Molecular analysis of magnetotactic bacteria and development of functional bacterial magnetic particles for nano-biotechnology.
T. Matsunaga (2007)
R. Blakemore (1975)
THE CELLULAR LOCATION OF ANTIGENS IN STREPTOCOCCI OF GROUPS D, N AND Q.
D. Smith (1964)
DNA extraction using modified bacterial magnetic particles in the presence of amino silane compound.
B. Yoza (2002)
This paper is referenced by
Magnetically based nanocarriers in drug delivery
E. Denkbaş (2016)
Novel Methods for the Synthesis of Magnetic Nanoparticles
S. Staniland (2014)
Characteristics and optimised fermentation of a novel magnetotactic bacterium, Magnetospirillum sp. ME-1
L. Ke (2018)
MamX encoded by the mamXY operon is involved in control of magnetosome maturation in Magnetospirillum gryphiswaldense MSR-1
J. Yang (2013)
Adaptive Evolution for Increased Biomass and Magnetic Nanoparticle Productivity of Magnetotactic Bacteria
Karthik Sekar (2015)
Nanotechnology, bionanotechnology and microbial cell factories
A. Villaverde (2010)
A Comparative Study of Receptor-Targeted Magnetosome and HSA-Coated Iron Oxide Nanoparticles as MRI Contrast-Enhancing Agent in Animal Cancer Model
Ebru Erdal (2017)
Effects of Electromagnetic Fields Exposure on the Production of Nanosized Magnetosome, Elimination of Free Radicals and Antioxidant Defense Systems in Magnetospirillum gryphiswaldense MSR-1
Hatami Giklou Jajan Leila (2019)
Manufacturing Man-Made Magnetosomes: High-Throughput In Situ Synthesis of Biomimetic Magnetite Loaded Nanovesicles.
P. K. Bakhshi (2016)
Complete Sequencing and Comparative Analysis of the Genomes of the First Magnetotactic Gammaproteobacteria Isolated in Pure Culture: Strains BW-2 and SS-5
Corey Geurink (2017)
Production of nanoparticles using organisms
H. Korbekandi (2009)
A Protein Corona Adsorbed to a Bacterial Magnetosome Affects Its Cellular Uptake
Wenjia Lai (2020)
Bacterial magnetic particles-polyethylenimine vectors deliver target genes into multiple cell types with a high efficiency and low toxicity
Wanjie Yang (2020)
Applications of magnetotactic bacteria and magnetosome for cancer treatment: A review emphasizing on practical and mechanistic aspects.
E. Alphandéry (2020)
Bacterial magnetic particles improve testes-mediated transgene efficiency in mice
C. Wang (2017)
Magnetotactic bacteria for cancer therapy
M. L. Fdez-Gubieda (2020)
An overview of heavy metal removal from wastewater using magnetotactic bacteria
I. Ali (2018)
Magnetotactic Bacteria, Magnetosomes, and Nanotechnology
D. Bazylinski (2014)
Reduction of Hg(II) to Hg(0) by Biogenic Magnetite from two Magnetotactic Bacteria
Songnian Liu (2018)
A Novel Highly Efficient Device for Growing Micro-Aerophilic Microorganisms
Maxime Fuduche (2019)
Designa Statistics-Based Experimental Magnetovibrio blakemorei Strain MV-1 and Growth by the Magnetotactic Vibrio Optimization of Magnetosome Production
Denise Maria Guimarães Freire (2014)
Improved methods for mass production of magnetosomes and applications: a review
A. Basit (2020)
Transcriptome analysis reveals physiological characteristics required for magnetosome formation in Magnetospirillum gryphiswaldense MSR-1.
X. Wang (2016)
Semicontinuous Culture of Magnetospirillum gryphiswaldense MSR-1 Cells in an Autofermentor by Nutrient-Balanced and Isosmotic Feeding Strategies
Y. Zhang (2011)
Magnetosomes: Bacterial Biosynthesis of Magnetic Nanoparticles and Potential Biomedical Applications
S. Staniland (2011)
The effect of iron-chelating agents on Magnetospirillum magneticum strain AMB-1: stimulated growth and magnetosome production and improved magnetosome heating properties
E. Alphandéry (2012)
Erratum to: The effect of iron-chelating agents on Magnetospirillum magneticum strain AMB-1: stimulated growth and magnetosome production and improved magnetosome heating properties
E. Alphandéry (2012)
Nanostructured bacterial materials for innovative medicines.
E. Rodríguez-Carmona (2010)
Bacterial Magnetosome: A Novel Biogenetic Magnetic Targeted Drug Carrier with Potential Multifunctions.
Jianbo Sun (2011)
A key time point for cell growth and magnetosome synthesis of Magnetospirillum gryphiswaldense based on real-time analysis of physiological factors
J. Yang (2013)
Enhanced antitumor efficacy of biocompatible magnetosomes for the magnetic hyperthermia treatment of glioblastoma
Raphaël Le Fèvre (2017)
Effects of Environmental Conditions on High-Yield Magnetosome Production by Magnetospirillum gryphiswaldense MSR-1
Leila Hatami-Giklou Jajan (2019)See more