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Stimulation Of Bone Formation By Intraosseous Injection Of Basic Fibroblast Growth Factor In Ovariectomised Rats

K. Nakamura, T. Kurokawa, I. Aoyama, Keigo Hanada, M. Tamura, H. Kawaguchi
Published 1998 · Medicine

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Summary. The effect on intraosseous bone formation of a single local injection of recombinant human basic fibroblast growth factor into trabecular bones was examined in ovariectomised osteoporotic rats. Fibroblast growth factor (400 μg), or the vehicle alone, was injected into the ilium at 16 weeks after ovariectomy or a simulated operation. Bone mineral density in the ovariectomised rats increased to a level similar to the latter at 2 weeks and reached a maximum at 8 weeks. After 8 weeks, BMD decreased slowly and the value at 24 weeks was still higher than that in the ovariectomised rats. Fibroblast growth factor stimulated osteoid formation in the first 2 weeks, bone volume reaching a peak at 8 weeks. From 8 to 12 weeks, bone resorption increased, resulting in decreases in bone volume to the levels of the group with simulated operations at 24 weeks. Structural analysis at 8 and 24 weeks showed that ovariectomy decreased the continuity of trabeculae and the injection of fibroblast growth factor restored it to levels higher than, or equal to, those who had the simulated operation. The present study demonstrated that intraosseous fibroblast growth factor given to ovariectomised rats restored bone volume and quality to the levels of the rats who had a simulated operation only.Résumé. L’effet d’une unique injection locale dans les os trabeculaires de facteur de croissance de base de fibroblaste humain recombinant (bFGF) sur la formation intra-osseuse a été examiné sur des rats ostéoporotiques ovariectomisés. L’injection bFGF (400 μg) ou véhicule seul (V) a été pratiquée dans l’ilion 16 semaines après ovariectomie (OVX) ou pseudo-opération (Sham). A 2 semaines, la densité osseuse moyenne des OVX+bFGF avait augmenté jusqu’à un niveau similaire aux Sham+V pour atteindre son maximum à 8 semaines, soit 19% de plus que les Sham+V. Au-delà de 8 semaines, la densité moyenne a décliné lentement, conservant à 24 semaines une valeur supérieure aux OVX+V. Le bFGF a stimulé la formation ostéoïde des 2 premières semaines. Le volume d’os minéralisé a atteint son sommet à 8 semaines. Entre 8 et 12 semaines, la résorption osseuse a été facilitée, conduisant à des baisses du volume osseux jusqu’aux niveaux Sham+V 24 semaines. Les analyses structurelles à 8 et 24 semaines ont montré que l’injection bFGF ramène à des niveaux égaux ou supérieurs à Sham+V la connectivité des trabeculae réduite par l’OVX. La présente étude établit qu’une application intraosseuse de bFGF après ovariectomie ramène le volume et la qualité osseuse au niveau des rats pseudo-opérés.
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