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Reduction Of Hexavalent Chromium By Cell-Free Extract Of Bacillus Sphaericus AND 303 Isolated From Serpentine Soil

Arundhati Pal, Sumana Dutta, A. Paul
Published 2005 · Chemistry, Medicine

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Cell-free extracts (CFEs) of chromium-resistant bacterium Bacillus sphaericus AND 303 isolated from serpentine soil of Andaman, India reduced Cr(VI) in in vitro condition, and the reductase activity was solely localized in the soluble cell-fractions (S12, S32, and S150). The enzyme was constitutive as the CFEs from cells grown in Cr(VI)-free and Cr(VI)-containing media reduced a more or less equal amount of Cr(VI). Optimum Cr(VI) reductase activity was obtained at an enzyme (S150) concentration equivalent to 4.56 mg protein/mL, 300 μM Cr(VI) and pH 6.0 after 30 min incubation at 30°C. The enzyme was heat labile; 80% of its activity was lost when exposed at 70°C for 15 min. Kinetics of Cr(VI) reductase activity fit well with the linearized Lineweaver-Burk plot and showed a Vmax of 1.432 μmol Cr(VI)/mg protein/min and Km of 158.12 μM Cr(VI). The presence of additional electron donors accelerated Cr(VI) reductase activity of CFE, and an increase of 28% activity over control was recorded with 1.0 μM NADH. Heavy metal ions such as Ni(II), Cu(II), and Cd(II) were strong inhibitors of Cr(VI) reductase unlike that of 100 μM Co(II), which retained 93% activity over control.
This paper references
Inducible periplasmic chromate reducing activity in Pseudomonas aeruginosa isolated from a leather tannery effluent
A. Ganguli (2001)
10.1016/0043-1354(95)00093-Z
Factors affecting hexavalent chromium reduction in pure cultures of bacteria
Yi-tin Wang (1995)
Purification and partial characterization of a chromate reductase from Bacillus.
J. Campos-García (1997)
10.1271/BBB1961.51.2417
Enzymatic Reduction of Hexavalent Chromium by Hexavalent Chromium Tolerant Pseudomonas ambigua G-1
H. Horitsu (1987)
10.1128/AEM.66.5.1788-1795.2000
Purification to Homogeneity and Characterization of a Novel Pseudomonas putida Chromate Reductase
C. H. Park (2000)
10.1007/s00253-003-1291-x
In vitro reduction of hexavalent chromium by a cell-free extract of Bacillus sp. ES 29 stimulated by Cu2+
F. Camargo (2003)
10.1128/AEM.59.11.3771-3777.1993
Characterization of enzymatic reduction of hexavalent chromium by Escherichia coli ATCC 33456.
H. Shen (1993)
10.1016/s0269-7491(96)90019-6
Biological degradation and bioremediation of toxic chemicals
H. Painter (1996)
10.1016/s0021-9258(19)52451-6
Protein measurement with the Folin phenol reagent.
O. H. Lowry (1951)
10.1016/J.MICRES.2004.08.001
Aerobic chromate reduction by chromium-resistant bacteria isolated from serpentine soil.
Arundhati Pal (2004)
10.1097/00010694-195002000-00009
Colorimetric Methods Of Analysis
F. D. Snell (1950)
10.1007/978-1-4612-2656-7_3
Environmental biochemistry of chromium.
M. Losi (1994)
Bacterial detoxification of toxic chromate
H Ohtake (1994)
10.1128/AEM.67.3.1076-1084.2001
Chromate Reduction by a Pseudomonad Isolated from a Site Contaminated with Chromated Copper Arsenate
J. McLean (2001)
10.1023/A:1008358816529
Aerobic chromate reduction by Bacillus subtilis
C. Garbisu (2004)
10.1128/JB.172.3.1670-1672.1990
Membrane-associated chromate reductase activity from Enterobacter cloacae.
P. C. Wang (1990)
10.1007/BF00871816
Hexavalent-chromium reduction by a chromate-resistantBacillus sp. strain
J. Campos (2004)
10.5860/choice.32-6244
Biological Degradation and Bioremediation of Toxic Chemicals
G. R. Chaudhry (1995)
10.1128/JB.174.16.5340-5345.1992
NAD(P)H-dependent chromium (VI) reductase of Pseudomonas ambigua G-1: a Cr(V) intermediate is formed during the reduction of Cr(VI) to Cr(III).
T. Suzuki (1992)
10.1111/J.1574-6976.2001.TB00581.X
Interactions of chromium with microorganisms and plants.
C. Cervantes (2001)



This paper is referenced by
10.1016/j.jenvman.2015.04.011
Bioremediation of hexavalent chromate using permeabilized Brevibacterium sp. and Stenotrophomonas sp. cells.
Shimei Ge (2015)
10.1016/J.RHISPH.2018.01.004
Antioxidant and chromium reductase assisted chromium (VI) reduction and Cr (III) immobilization by the rhizospheric Bacillus helps in the remediation of Cr (VI) and growth promotion of soybean crop
P. A. Wani (2018)
10.1111/1574-6968.12481
ArsH from Synechocystis sp. PCC 6803 reduces chromate and ferric iron.
Xi-Mei Xue (2014)
10.5897/AJMR12.143
Hexavalent chromium reduction by metal resistant and halotolerant Planococcus maritimus VITP21
S. Subramanian (2012)
10.1186/1471-2180-10-221
Characterization and genomic analysis of chromate resistant and reducing Bacillus cereus strain SJ1
Minyan He (2010)
10.5772/60660
Hexavalent Chromium (VI) Removal by Penicillium sp. IA-01
I. Acosta-Rodríguez (2015)
10.5772/24311
Biological Cr(VI) Reduction: Microbial Diversity, Kinetics and Biotechnological Solutions to Pollution
E. Chirwa (2011)
10.1016/j.chemosphere.2016.04.101
Evaluation of chromate reductase activity in the cell-free culture filtrate of Arthrobacter sp. SUK 1201 isolated from chromite mine overburden.
S. Dey (2016)
10.1016/j.jhazmat.2010.09.072
Characterization and genomic analysis of a highly chromate resistant and reducing bacterial strain Lysinibacillus fusiformis ZC1.
Minyan He (2011)
10.1007/s12223-014-0304-8
Bacterial mechanisms for Cr(VI) resistance and reduction: an overview and recent advances
M. Ahemad (2014)
Isolation and Characterization of Heavy Metal Resistant Bacteria from Barak River Contaminated with Pulp Paper Mill Effluent
South Assam (2012)
Evaluation of heavy metal tolerant bacterial isolates for effluent treatment
Gaganpreet Kaur (2016)
Biogenèse de la pellicule chez Shewanella oneidensis
Cyril Gambari (2018)
10.1007/s12010-008-8515-6
Hexavalent Chromium Reduction by Free and Immobilized Cell-free Extract of Arthrobacter rhombi-RE
R. Elangovan (2010)
10.22102/JAEHR.2018.82265.1014
Chromium bioremediation by Alcaligenes sp. strain newly isolated from chromite mine of Sabzevar
Mohsen Mesbahi-Nowrouzi (2017)
10.3303/CET1335139
Remobilization of Trivalent Chromium and the Regeneration of In Situ Permeable Reactive Barriers during Operation
L. A. Kaimbi (2013)
10.1016/j.jhazmat.2009.09.134
Chromium (VI) biotransformation by beta- and gamma-Proteobacteria from natural polluted environments: a combined biological and chemical treatment for industrial wastes.
L. Garavaglia (2010)
10.1016/J.CHEMOSPHERE.2006.11.008
Chromium(VI) resistance and removal by actinomycete strains isolated from sediments.
M. A. Polti (2007)
10.1080/10889868.2011.598488
Bioremediation of Cr(VI) from Chromium-Contaminated Wastewater by Free and Immobilized Cells of Cellulosimicrobium cellulans KUCr3
Swagata Chatterjee (2011)
10.1002/JCTB.1882
Biological Cr(VI) reduction in a trickling filter under continuous operation with recirculation
E. Dermou (2008)
10.1128/AEM.02208-18
Chromate Resistance Mechanisms in Leucobacter chromiiresistens
G. Sturm (2018)
10.2323/JGAM.58.263
Isolation, identification and characterization of a glyphosate-degrading bacterium, Bacillus cereus CB4, from soil.
Jieyu Fan (2012)
10.2166/wst.2009.349
Microbial culture dynamics and chromium (VI) removal in packed-column microcosm reactors.
P. Molokwane (2009)
Bioremediation of Chromium Contaminated Soil: Reduction and Optimization under Laboratory conditions
Dusengimana Claire (2016)
10.1007/978-3-7091-0730-0_4
Chromium–Plant-Growth-Promoting Rhizobacteria Interactions: Toxicity and Management
M. S. Khan (2012)
10.1002/jobm.200800239
Bioremediation of chromium(VI) contaminated soil by Streptomyces sp. MC1
M. A. Polti (2009)
10.1007/s00203-010-0622-4
Chromate reductase activity of the Paracoccusdenitrificans ferric reductase B (FerB) protein and its physiological relevance
Vojtěch Sedláček (2010)
10.1002/jobm.200800046
Chromate resistance, transport and bioreduction by Exiguobacterium sp. ZM‐2 isolated from agricultural soil irrigated with tannery effluent
M. Z. Alam (2008)
10.1016/j.jwpe.2020.101748
Reduction of hexavalent chromium by Microbacterium paraoxydans isolated from tannery wastewater and characterization of its reduced products
S. Mishra (2020)
10.1007/s11270-015-2356-z
Bioreduction of Chromate by an Isolated Bacillus anthracis Cr-4 with Soluble Cr(III) Product
W. Xu (2015)
10.1007/s11274-018-2510-z
Isolation and characterization of chromium(VI)-reducing bacteria from tannery effluents and solid wastes
M. Kabir (2018)
10.1016/j.jhazmat.2009.07.085
Removal of mercury from its aqueous solution using charcoal-immobilized papain (CIP).
S. Dutta (2009)
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