Online citations, reference lists, and bibliographies.
← Back to Search

H2O2 Involvement In Polyamine-Induced Cell Death In Tobacco Leaf Discs

M. Iannone, E. Rosales, M. D. Groppa, M. Benavides
Published 2013 · Biology

Cite This
Download PDF
Analyze on Scholarcy
Share
The response of tobacco (Nicotiana tabacum L.) wild-type SR1 leaf discs in terms of reactive oxygen species (ROS) formation and cell death occurrence was evaluated after exposure to the polyamines (PAs) putrescine (Put), spermidine (Spd), and spermine (Spm). Although NADPH oxidase-like enzyme activity was inhibited by all PAs at 3 or 21 h of treatment, H2O2 content increased significantly in a time- and concentration-dependent manner, suggesting that H2O2 accumulation was linked to the activity of other ROS-generating enzymes. Polyamine oxidase (PAO) activity, which increased markedly upon application of Spd or Spm, is a prime candidate for the increased H2O2 accumulation. Except for 0.1 mM Put, which maintained guaiacol peroxidase (GPOX) and catalase (CAT) activities at the same level as the control, the other PA treatments decreased CAT, ascorbate peroxidase, and GPOX activities at 21 h, contributing to the H2O2 increase. Esterase activity and Evans blue staining, two cell death parameters, were negatively affected at 3 h of treatment with 1 mM Spd and with both concentrations of Spm, whereas at 21 h there was an increase in cell death with both concentrations of the three PAs, except for 0.1 mM Put, which did not alter those parameters. The expression of the senescence-associated cysteine protease gene CP1 was measured to monitor senescence, a physiological cell death process. Application of all PAs increased the expression of the gene, except for 0.1 mM Put, which decreased its expression at 21 h. This result was in agreement with the prevention of cell death exerted by Put and evidenced by Evans blue staining, esterase activity, and electrolyte release.
This paper references
10.1016/J.TPLANTS.2005.12.009
Functions of amine oxidases in plant development and defence.
A. Cona (2006)
10.1016/j.plaphy.2010.02.009
Differential and functional interactions emphasize the multiple roles of polyamines in plants.
A. K. Handa (2010)
10.1016/0003-9861(68)90654-1
Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation.
R. L. Heath (1968)
10.1111/j.1399-3054.2008.01049.x
Transgenic tobacco plants overexpressing polyamine oxidase are not able to cope with oxidative burst generated by abiotic factors.
P. Moschou (2008)
10.1007/s002990050729
A new methodology for plant cell viability assessment using intracellular esterase activity
N. Steward (1999)
10.1104/pp.108.122945
Putrescine Is Involved in Arabidopsis Freezing Tolerance and Cold Acclimation by Regulating Abscisic Acid Levels in Response to Low Temperature1
Juan C. Cuevas (2008)
10.1093/jxb/ers031
The soluble proteome of tobacco Bright Yellow-2 cells undergoing H2O2-induced programmed cell death
C. Vannini (2012)
10.1111/J.1365-313X.2004.02092.X
Arabidopsis thaliana plants overexpressing thylakoidal ascorbate peroxidase show increased resistance to Paraquat-induced photooxidative stress and to nitric oxide-induced cell death.
I. Murgia (2004)
10.1016/S0168-9452(01)00432-0
Polyamines as protectors against cadmium or copper-induced oxidative damage in sunflower leaf discs
M. D. Groppa (2001)
10.1046/J.1365-313X.1999.00370.X
Expression of cysteine proteinase during developmental events associated with programmed cell death in brinjal.
F. Xu (1999)
10.1126/science.1152505
Local Positive Feedback Regulation Determines Cell Shape in Root Hair Cells
S. Takeda (2008)
10.1007/s00425-010-1130-0
Polyamines: molecules with regulatory functions in plant abiotic stress tolerance
R. Alcázar (2010)
10.1093/PCP/PCI252
Polyamines induce rapid biosynthesis of nitric oxide (NO) in Arabidopsis thaliana seedlings.
N. Tun (2006)
10.1007/BF00037585
An improved method for monitoring cell death in cell suspension and leaf disc assays using evans blue
C. Jacyn Baker (2004)
Molecular cloning. A laboratory manual Resistance response physiology and signal transduction
J Sambrook (1989)
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
10.1104/pp.111.179671
Polyamines Interact with Hydroxyl Radicals in Activating Ca2+ and K+ Transport across the Root Epidermal Plasma Membranes1[W]
I. Zepeda-Jazo (2011)
Effect of spermine, atrazine and combination between them on some endogenous protective systems and stress markers in plants
I. Sergiev (1997)
10.1016/1369-5266(88)80051-7
Resistance response physiology and signal transduction.
D. Scheel (1998)
10.1152/PHYSREV.1979.59.3.527
Hydroperoxide metabolism in mammalian organs.
B. Chance (1979)
10.1042/BJ3130549
Spermine suppresses the activation of human neutrophil NADPH oxidase in cell-free and semi-recombinant systems.
K. Ogata (1996)
10.1146/ANNUREV.ARPLANT.55.031903.141701
Reactive oxygen species: metabolism, oxidative stress, and signal transduction.
K. Apel (2004)
10.1016/b978-0-12-547150-3.50015-3
A Laboratory manual
M. Ashburner (1989)
10.1016/j.jplph.2008.12.014
Spatial patterns of senescence and development-dependent distribution of reactive oxygen species in tobacco (Nicotiana tabacum) leaves.
E. Niewiadomska (2009)
10.1016/j.jplph.2009.06.012
Hydrogen peroxide- and nitric oxide-induced systemic antioxidant prime-like activity under NaCl-stress and stress-free conditions in citrus plants.
G. Tanou (2009)
Roubelakis-Angelakis KA (2008b) Spermidine exodus and oxidation in the apoplast induced by abiotic stress is responsible for H 2 O 2 signatures that direct tolerance responses in tobacco
Pn Moschou
10.1007/s00726-011-1001-4
Polyamines modulate nitrate reductase activity in wheat leaves: involvement of nitric oxide
E. Rosales (2011)
10.1111/J.1365-313X.2004.02160.X
Molecular events in senescing Arabidopsis leaves.
J. Lin (2004)
10.1104/PP.82.4.1169
Some Enzymes of Hydrogen Peroxide Metabolism in Leaves and Root Nodules of Medicago sativa.
M. Becana (1986)
10.1083/jcb.200409170
Hydrogen peroxide as a signal controlling plant programmed cell death
T. Gechev (2005)
10.1093/JXB/ERL092
Inhibition of the ethylene response by 1-MCP in tomato suggests that polyamines are not involved in delaying ripening, but may moderate the rate of ripening or over-ripening.
A. Tassoni (2006)
10.1016/j.plaphy.2010.08.016
Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants.
S. Gill (2010)
10.1073/PNAS.0308095100
Expression of an active tobacco mitogen-activated protein kinase kinase kinase enhances freezing tolerance in transgenic maize.
Huixia Shou (2004)
10.1007/s00726-007-0501-8
Polyamines and abiotic stress: recent advances
M. D. Groppa (2007)
10.1016/0003-9861(68)90523-7
Photoperoxidation in isolated chloroplasts. II. Role of electron transfer.
R. L. Heath (1968)
10.1105/tpc.108.059733
Spermidine Exodus and Oxidation in the Apoplast Induced by Abiotic Stress Is Responsible for H2O2 Signatures That Direct Tolerance Responses in Tobacco[W]
P. Moschou (2008)
10.1111/J.1469-8137.2006.01828.X
Transcription of ethylene perception and biosynthesis genes is altered by putrescine, spermidine and aminoethoxyvinylglycine (AVG) during ripening in peach fruit (Prunus persica).
V. Ziosi (2006)
10.1046/J.1365-313X.2003.01655.X
Changes in hydrogen peroxide homeostasis trigger an active cell death process in tobacco.
J. Dat (2003)
10.1016/j.pbi.2011.07.014
Respiratory burst oxidases: the engines of ROS signaling.
N. Suzuki (2011)
Boveris A (1979) Hydroperoxide metabolism in mammalian organs
B Chance (1979)
10.1002/9780470110171.CH14
The assay of catalases and peroxidases.
A. Maehly (1954)
10.1016/j.phytochem.2008.05.015
Enhancement of spermidine content and antioxidant capacity in transgenic pear shoots overexpressing apple spermidine synthase in response to salinity and hyperosmosis.
Lixiong He (2008)
10.1046/J.1365-313X.1997.12020267.X
A programmed cell death pathway activated in carrot cells cultured at low cell density
P. McCabe (1997)
10.1104/pp.111.173153
Perturbation of Polyamine Catabolism Can Strongly Affect Root Development and Xylem Differentiation1[W]
A. Tisi (2011)
A Guide to the Polyamines
S. Cohen (1998)
10.1016/j.plaphy.2010.02.001
Plant amine oxidases "on the move": an update.
R. Angelini (2010)
10.1007/978-1-4684-5637-0
Progress in Polyamine Research
V. Zappia (1988)
10.1006/BBRC.2000.2601
Polyamines: mysterious modulators of cellular functions.
K. Igarashi (2000)
10.1007/s00425-004-1400-9
Polyamines inhibit NADPH oxidase-mediated superoxide generation and putrescine prevents programmed cell death induced by polyamine oxidase-generated hydrogen peroxide
A. Papadakis (2004)
10.1023/B:MCBI.0000038227.91813.79
Hydroxyl radical scavenging and singlet oxygen quenching properties of polyamines
K. Das (2004)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
10.1104/pp.103.024737
Induction of Hypersensitive Cell Death by Hydrogen Peroxide Produced through Polyamine Degradation in Tobacco Plants1
Hiroshi Yoda (2003)
10.1046/J.1365-313X.2003.01923.X
Spermine signalling in tobacco: activation of mitogen-activated protein kinases by spermine is mediated through mitochondrial dysfunction.
Yoshihiro Takahashi (2003)
10.1046/J.1365-313X.2002.01342.X
The plasma membrane oxidase NtrbohD is responsible for AOS production in elicited tobacco cells.
F. Simon-Plas (2002)
Fatty acid hydroperoxides
JL Montillet (2005)
Curr Opin Plant Biol
The di- and polyamine oxidase of plants. In: Zappia V, Pegg AE (eds) Progress in polyamine research. Novel biochemical, pharmacological and clinical aspects
TA Smith (1988)
10.1105/tpc.105.033589
Redox Homeostasis and Antioxidant Signaling: A Metabolic Interface between Stress Perception and Physiological Responses
C. Foyer (2005)
10.1007/s11103-009-9459-0
Polyamines as a common source of hydrogen peroxide in host- and nonhost hypersensitive response during pathogen infection
Hiroshi Yoda (2009)
10.1093/jxb/ers202
The polyamines and their catabolic products are significant players in the turnover of nitrogenous molecules in plants.
P. N. Moschou (2012)
10.1111/j.1365-3040.2011.02387.x
Redox regulation in plant programmed cell death.
M. D. de Pinto (2012)
10.1016/j.phytochem.2008.07.016
Nitric oxide, polyamines and Cd-induced phytotoxicity in wheat roots.
M. D. Groppa (2008)
10.1152/AJPCELL.00451.2006
Spermidine/spermine N1-acetyltransferase overexpression in kidney epithelial cells disrupts polyamine homeostasis, leads to DNA damage, and causes G2 arrest.
K. Zahedi (2007)
10.1104/pp.105.059907
Fatty Acid Hydroperoxides and H2O2 in the Execution of Hypersensitive Cell Death in Tobacco Leaves1[w]
Jean-Luc Montillet (2005)
J Plant Growth Regul
(2013)
10.1093/OXFORDJOURNALS.PCP.A076232
Hydrogen Peroxide is Scavenged by Ascorbate-specific Peroxidase in Spinach Chloroplasts
Y. Nakano (1981)
10.1007/s00709-009-0097-9
Reactive oxygen species formation and cell death in catalase-deficient tobacco leaf disks exposed to cadmium
M. Iannone (2009)
10.1111/j.1399-3054.2008.01090.x
Reactive oxygen signaling and abiotic stress.
G. Miller (2008)
10.1007/BF00387695
Dielectric measurement to monitor the growth and the physiological states of biological cells
R. Matanguihan (1994)
10.1007/978-1-4684-5637-0_51
The DI- and polyamine oxidases of plants.
T. Smith (1988)
The assay of catalase and peroxidase Methods of biochemical analysis
Ac Maehly (1954)
10.1016/S0014-5793(98)01208-3
UV‐C radiation induces apoptotic‐like changes in Arabidopsis thaliana
A. Danon (1998)
10.1016/j.biotechadv.2011.01.003
Polyamines: natural and engineered abiotic and biotic stress tolerance in plants.
S. S. Hussain (2011)
10.1104/PP.124.1.431
Involvement of polyamines in the chilling tolerance of cucumber cultivars.
W. Shen (2000)
10.1016/J.CBPA.2007.01.648
Nitric oxide, stomatal closure and abiotic stress
S. Neill (2007)
Hydrogen Peroxide in Plants:a Versatile Molecule of the Reactive Oxygen Species Network
M. Key (2008)



This paper is referenced by
10.3389/fpls.2017.00203
Interaction of Polyamines, Abscisic Acid, Nitric Oxide, and Hydrogen Peroxide under Chilling Stress in Tomato (Lycopersicon esculentum Mill.) Seedlings
Qiannan Diao (2017)
10.1556/0806.47.2019.28
Modulation of Polyamines during Grain Development under Different Concentrations of Nitrogen in Wheat
Bhavpreet Kaur (2019)
10.1016/J.SCIENTA.2019.108788
Changes in polyamines during bud dormancy in almond cultivars differing in their flowering date
Somayeh Naseri (2019)
10.1093/aob/mcw111
Silicon-moderated K-deficiency-induced leaf chlorosis by decreasing putrescine accumulation in sorghum.
Daoqian Chen (2016)
10.1016/j.foodchem.2019.125303
Exogenous polyamines enhance resistance to Alternaria alternata by modulating redox homeostasis in apricot fruit.
Yong-cai Li (2019)
10.1016/j.plaphy.2019.08.028
Crosstalk among polyamines, phytohormones, hydrogen peroxide, and phenylethanoid glycosides responses in Scrophularia striata to Cd stress.
E. Beshamgan (2019)
10.3389/fpls.2016.01343
Hydrogen Peroxide and Polyamines Act as Double Edged Swords in Plant Abiotic Stress Responses
K. Gupta (2016)
10.22080/JGR.2015.1169
Biochemical and Cellular Response of Catharanthus roseus Callus Cells to Cadmium Toxicity
M. H. Abnosi (2015)
10.1556/0806.44.2016.028
Syringaldazine peroxidase stimulates lignification by enhancing polyamine catabolism in wheat during heat and drought stress
S. Bala (2016)
10.1007/978-981-13-8383-0_2
Manufacturing and Quality Control of Inoculants from the Paradigm of Circular Agriculture
Inés García de Salamone (2019)
10.1631/jzus.B1600102
Nitric oxide induced by polyamines involves antioxidant systems against chilling stress in tomato (Lycopersicon esculentum Mill.) seedling
Qian-nan Diao (2016)
10.3389/fphys.2015.00280
Polyamine regulates tolerance to water stress in leaves of white clover associated with antioxidant defense and dehydrin genes via involvement in calcium messenger system and hydrogen peroxide signaling
Z. Li (2015)
10.3389/fphys.2016.00052
Corrigendum: Polyamine regulates tolerance to water stress in leaves of white clover associated with antioxidant defense and dehydrin genes via involvement in calcium messenger system and hydrogen peroxide signaling
Z. Li (2016)
10.1016/j.plaphy.2018.05.002
Spermidine-mediated hydrogen peroxide signaling enhances the antioxidant capacity of salt-stressed cucumber roots.
J. Wu (2018)
10.1007/s11240-015-0901-5
Overexpression of S-adenosylmethionine synthetase 1 enhances tomato callus tolerance to alkali stress through polyamine and hydrogen peroxide cross-linked networks
B. Gong (2015)
10.1007/s00709-018-1227-z
Reactive oxygen species and nitric oxide are involved in polyamine-induced growth inhibition in wheat plants
Laura Recalde (2018)
10.1016/j.plaphy.2018.07.004
Water stress alleviation by polyamines and phenolic compounds in Scrophularia striata is mediated by NO and H2O2.
Hadi Falahi (2018)
Semantic Scholar Logo Some data provided by SemanticScholar