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Fatty Acid Patterns Of Phospholipids And Lipopolysaccharides In The Characterisation Of Microbial Communities In Soil: A Review

L. Zelles
Published 1999 · Biology

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Abstract This review discusses the analysis of whole-community phospholipid fatty acid (PLFA) profiles and the composition of lipopolysaccharides in order to assess the microbial biomass and the community structure in soils. For the determination of soil microbial biomass a good correlation was obtained between the total amount of PLFAs and the microbial biomass measured with methods commonly used for determinations such as total adenylate content and substrate-induced respiration. Generally, after the application of multivariate statistical analyses, whole-community fatty acid profiles indicate which communities are similar or different. However, in most cases, the organisms accounting for similarity or difference cannot be determined, and therefore artefacts could not be excluded. The fatty acids used to determine the biomass vary from those which determine the community structure. Specific attention has to be paid when choosing extraction methods in order to avoid the liberation of fatty acids from non-living organic material and deposits, and to exclude the non-target selection of lipids from living organisms, as well. By excluding the fatty acids which were presumed to be common and widespread prior to multivariate statistical analysis, estimates were improved considerably. Results from principal component analysis showed that determining the levels of fatty acids present in both low and high concentrations is essential in order to correctly identify microorganisms and accurately classify them into taxonomically defined groups. The PLFA technique has been used to elucidate different strategies employed by microorganisms to adapt to changed environmental conditions under wide ranges of soil types, management practices, climatic origins and different perturbations. It has been proposed that the classification of PLFAs into a number of chemically different subgroups should simplify the evaluating procedure and improve the assessment of soil microbial communities, since then only the subgroups assumed to be involved in key processes would be investigated.
This paper references
10.1016/0014-5793(72)80140-6
Structural analysis of molecular species of nocardomycolic acids fromNocardia erythropolis by the combined system of gas chromatography and mass spectrometry
I. Yano (1972)
10.1016/0167-7012(91)90018-L
Microbial biomass measured as total lipid phosphate in soils of different organic content
Å. Frostegård (1991)
Profiling of complex microbial populations by denaturating gradient gel electrophoresis of polymerase chain reaction-amplified genes encoding for 16 S rRNA
GE Muyzer (1993)
Tunlid A (1993a) Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty acid analysis
A Frostegård (1993)
10.1007/978-3-642-60694-6_6
Diversity of Anthropogenically Influenced or Disturbed Soil Microbial Communities
E. Laczko (1997)
10.1007/978-94-011-0479-1
The Significance and Regulation of Soil Biodiversity
H. Collins (1995)
10.1128/JB.177.15.4566-4570.1995
Chemical characterization of the ester-linked 3-hydroxy fatty acyl-containing lipids in Mycobacterium tuberculosis.
S. Alugupalli (1995)
Lipids in bacterial taxonomy. In: O’Leary WM (ed) Practical handbook of microbiology
MP Lechevalier (1989)
Fungal lipids
DM Lösel (1988)
Mikrobiologie von Bodenaggregaten eines Pelosols : Abhängigkeiten von Aggregatgröße, Wasserpotential und Substratverfügbarkeit
B. P. Albers (1995)
Fettsäuremuster von Phospholipiden und Lipopolysacchariden als Indikatoren für Struktur von Microorganismengesellschaften in Böden
B Albers (1994)
10.1099/00207713-47-1-191
Taxonomic study of aromatic-degrading bacteria from deep-terrestrial-subsurface sediments and description of Sphingomonas aromaticivorans sp. nov., Sphingomonas subterranea sp. nov., and Sphingomonas stygia sp. nov.
D. Balkwill (1997)
10.1007/978-3-642-60694-6_7
Impact of Fertilizers on the Humus Layer Microbial Community of Scots Pine Stands Growing Along a Gradient of Heavy Metal Pollution
H. Fritze (1997)
10.1007/s003740050496
Phospholipid fatty acid profiles as indicators for the microbial community structure in soils along a climatic transect in the Judean Desert
Y. Steinberger (1999)
Microbial distribution in soil – new techniques
TW Federle (1986)
10.1016/0038-0717(93)90113-P
Shifts in the structure of soil microbial communities in limed forests as revealed by phospholipid fatty acid analysis
Å. Frostegård (1993)
10.1002/JPLN.1998.3581610410
New quality of assessment of microbial diversity in arable soils using molecular and biochemical methods
M. Schloter (1998)
10.1128/AEM.60.7.2421-2430.1994
Effects of sieving, storage, and incubation temperature on the phospholipid Fatty Acid profile of a soil microbial community.
S. O. Petersen (1994)
10.1016/0167-7012(86)90023-0
Determination of monosaturated fatty acid double-bond position and geometry for microbial monocultures and complex consortia by capillary GC-MS of their dimethyl disulphide adducts
P. Nichols (1986)
Fatty acid patterns of phospholipids and lipopolysaccharides
L Zelles (1994)
10.1111/J.1574-6941.1998.TB00501.X
Phospholipid fatty acid profiles and carbon utilization patterns for analysis of microbial community structure under field and greenhouse conditions
A. M. Ibekwe (1998)
Chemotaxonomic use of lipids – an overview
H Lechevalier (1988)
Diversity of anthropogeni - cally influenced or disturbed soil microbial communities
E Laczko (1997)
10.1128/AEM.59.11.3605-3617.1993
Phospholipid Fatty Acid composition, biomass, and activity of microbial communities from two soil types experimentally exposed to different heavy metals.
A. Frostegård (1993)
Mycobacterium and other actinomycetes
PJ Brennan (1988)
10.1016/0038-0717(93)90075-M
Fractionation of fatty acids derived from soil lipids by solid phase extraction and their quantitative analysis by GC-MS
L. Zelles (1993)
10.2307/1310976
Lipid analysis in microbial ecology: quantitative approaches to the study of microbial communities.
J. Vestal (1989)
10.1016/0038-0717(94)90300-X
Bacterial communities in peat in relation to botanical composition as revealed by phospholipid fatty acid analysis
Peter Borga (1994)
Fatty acid patterns of microbial phospholipids and lipopolysaccharides
L Zelles (1996)
10.1016/S0038-0717(97)00062-X
Changes in soil microbial properties and phospholipid fatty acid fractions after chloroform fumigation
L. Zelles (1997)
10.1007/978-94-009-0215-2_7
The use of phospholipid fatty acids to determine microbial community structure
R. Findlay (1996)
10.1016/0167-7012(93)90068-S
Sequential alkaline saponification/acid hydrolysis/esterification: a one-tube method with enhanced recovery of both cyclopropane and hydroxylated fatty acids
W. Mayberry (1993)
10.2136/SSSAJ1997.03615995006100020015X
Compositional and functional shifts in microbial communities due to soil warming
G. Zogg (1997)
10.1016/0045-6535(94)90136-8
Fatty acid patterns of phospholipids and lipopolysaccharids in environmental samples
L. Zelles (1994)
10.1007/978-94-011-5989-0
Lipids in plants and microbes
J. Harwood (1984)
The use of phospholipid analysis to estimate bacterial and fungal biomass in soils
A Frostegård (1996)
10.1128/AEM.63.6.2224-2231.1997
Dynamics of a microbial community associated with manure hot spots as revealed by phospholipid fatty acid analyses.
A. Frostegård (1997)
10.1016/S0045-6535(97)00155-0
Phospholipid fatty acid profiles in selected members of soil microbial communities.
L. Zelles (1997)
10.1016/S0045-6535(99)00131-9
Identification of single cultured micro-organisms based on their whole-community fatty acid profiles, using an extended extraction procedure.
L. Zelles (1999)
10.1007/978-1-4757-2191-1_34
The Genus Bacteroides and Related Taxa
H. Shah (1992)
10.1016/0038-0717(92)90191-Y
Signature fatty acids in phospholipids and lipopolysaccharides as indicators of microbial biomass and community structure in agricultural soils
L. Zelles (1992)
10.1016/S0021-9673(01)95986-2
Location of double bonds in polyunsaturated fatty acids by gas chromatography-mass spectrometry after 4,4-dimethyloxazoline derivatization
L. Fay (1991)
10.1128/AEM.56.3.782-787.1990
High diversity in DNA of soil bacteria.
V. Torsvik (1990)
Characterisation of microbial consortia in paddy soil by analysis of phospholipid fatty acids
Q Bai (1999)
10.1016/0038-0717(95)00100-X
Changes in microbial community structure during long-term incubation in two soils experimentally contaminated with metals
Å. Frostegård (1996)
10.1016/0038-0717(83)90095-0
The degradation of 14C-labelled phosphatidyl choline in soil
T. Tollefson (1983)
10.1002/BMS.1200050104
Separation and analysis of molecular species of mycolic acids in Nocardia and related taxa by gas chromatography mass spectrometry.
I. Yano (1978)
10.1128/AEM.59.3.695-700.1993
Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.
G. Muyzer (1993)
Modelluntersuchungen zur Stickstoffdynamik in Schwarzerden mit unterschiedlicher Düngungsgeschichte
R Rackwitz (1997)
Structure and distribution of plant acyl lipids
C Hitchcock (1975)
10.1007/978-94-011-0479-1_7
The functional significance of the microbial biomass in organic and conventionally managed soils
M. Wander (1995)
Phospholipid fatty acid analysis to detect changes in soil microbial community structure
A Frostegård (1995)
10.1139/o59-099
A rapid method of total lipid extraction and purification.
E. Bligh (1959)
10.2307/2269568
Soil Microbial Communities Beneath Populus Grandidentata Grown Under Elevated Atmospheric CO2
D. Zak (1996)
10.1016/0038-0717(94)90175-9
Microbial biomass, metabolic activity and nutritional status determined from fatty acid patterns and poly-hydroxybutyrate in agriculturally-managed soils
L. Zelles (1994)
10.1099/00207713-43-4-735
Revised Taxonomy of the Methanotrophs: Description of Methylobacter gen. nov., Emendation of Methylococcus, Validation of Methylosinus and Methylocystis Species, and a Proposal that the Family Methylococcaceae Includes Only the Group I Methanotrophs
J. Bowman (1993)
Analysis of microbial communities within sediments using biomarkers
R. J. Parkes (1987)
10.3354/MEPS045069
Callianassa trilobata (Crustacea: Thalassinidea) influences abundance of meiofauna and biomass, composition, and physiologic state of microbial communities within its burrow
F. Dobbs (1988)
10.1128/AEM.60.7.2483-2493.1994
Accuracy, reproducibility, and interpretation of Fatty Acid methyl ester profiles of model bacterial communities.
S. K. Haack (1994)
10.1111/J.1574-6968.1991.TB04732.X
A comparison of phospholipid and chloroform fumigation analyses for biomass in soil: potentials and limitations
S. O. Petersen (1991)
10.1128/AEM.62.2.420-428.1996
Phospholipid Fatty Acid Composition and Heavy Metal Tolerance of Soil Microbial Communities along Two Heavy Metal-Polluted Gradients in Coniferous Forests.
T. Pennanen (1996)
10.1007/BF00422226
Extraction, distribution and biodegradation of bacterial lipopolysaccharides in estuarine sediments
J. N. Saddler (2004)
10.1016/S0038-0717(97)00019-9
Seasonality of the soil biota of grazed and ungrazed hill grasslands
R. Bardgett (1997)
10.1128/MMBR.59.1.143-169.1995
Phylogenetic identification and in situ detection of individual microbial cells without cultivation.
R. Amann (1995)
10.1099/00221287-137-1-197
POLAR LIPIDS AND FATTY ACIDS OF PSEUDOMONAS CARYOPHYLLI, PSEUDOMONAS GLADIOLI AND PSEUDOMONAS PICKETTII
L. Galbraith (1991)
10.3109/10408417709102311
Lipids in Bacterial Taxonomy - A Taxonomist's View
Lechevalier Mp (1977)
10.1007/s002489900082
Impacts of Carbon and Flooding on Soil Microbial Communities: Phospholipid Fatty Acid Profiles and Substrate Utilization Patterns
D. Bossio (1998)
The genus Nocardiopsis
R. M. Kroppenstedt (1992)
10.2307/2405027
Methods in Applied Soil Microbiology and Biochemistry
K. Alef (1995)
Accumulation of poly-b-hydroxibutyrate in methane enriched, halogenated hydrocarbon-degrading soil columns: implications for microbial community structure and nutritional status
PD Nichols (1989)
10.1099/00207713-46-4-1042
Description of chlorophenol-degrading Pseudomonas sp. strains KF1T, KF3, and NKF1 as a new species of the genus Sphingomonas, Sphingomonas subarctica sp. nov.
L. Nohynek (1996)
10.1128/AEM.63.4.1476-1482.1997
Variation in microbial community structure in two boreal peatlands as determined by analysis of phospholipid Fatty Acid profiles.
I. Sundh (1997)
10.1128/JB.178.21.6223-6226.1996
Isolation and composition of inositolphosphorylceramide-type sphingolipids of hyphal forms of Candida albicans.
G. Wells (1996)
Biochemical analysis of biomass community structure nutritional status and metabolic activity of microbial communities in soil
A. Tunlid (1992)
10.1128/AEM.63.1.233-238.1997
Microbial communities of continuously cropped, irrigated rice fields.
W. Reichardt (1997)
10.1038/216205a0
Microbial Lipids
D. Gompertz (1967)
10.1007/978-3-662-07548-7
Gram-Positive Bacteria
G. Pozzi (1997)
10.2307/4117280
Recent Advances in the Chemistry and Biochemistry of Plant Lipids
T. Reynolds (1977)
10.1128/AEM.58.12.4026-4031.1992
Soil Bacterial Biomass, Activity, Phospholipid Fatty Acid Pattern, and pH Tolerance in an Area Polluted with Alkaline Dust Deposition.
E. Bååth (1992)
10.1128/AEM.44.5.1170-1177.1982
Sensitive assay, based on hydroxy fatty acids from lipopolysaccharide lipid A, for Gram-negative bacteria in sediments.
J. Parker (1982)
10.1093/OXFORDJOURNALS.JBCHEM.A132951
Regulation of cell wall mycolic acid biosynthesis in acid-fast bacteria. I. Temperature-induced changes in mycolic acid molecular species and related compounds in Mycobacterium phlei.
S. Toriyama (1980)



This paper is referenced by
10.2323/JGAM.54.205
Microbial community in the rhizosphere of young maize seedlings is susceptible to the impact of introduced pseudomonads as indicated by FAME analysis.
J. Kozdrój (2008)
10.1371/journal.pone.0081178
Survival of Escherichia coli O157:H7 in Soils from Jiangsu Province, China
T. Zhang (2013)
10.1007/s10705-010-9386-0
Microbial nitrogen dynamics in south central Chilean agricultural and forest ecosystems located on an Andisol
D. Huygens (2010)
10.1007/s11356-017-9529-9
Effects of residue incorporation and plant growth on soil labile organic carbon and microbial function and community composition under two soil moisture levels
Zengqiang Li (2017)
10.1007/S11707-009-0009-5
Distributions of phospholipid and glycolipid fatty acids in two strains of different functional Erythrobacter sp. isolated from South China Sea
H. Yang (2009)
10.1007/s12223-012-0136-3
PLFA profiles for microbial community monitoring in anaerobic digestion
T. Schwarzenauer (2012)
10.1016/J.SOILBIO.2006.01.018
Microbial diversity in three floodplain soils at the Elbe River (Germany)
J. Rinklebe (2006)
10.25773/V5-QNS9-Y653
Sediment and Organic Carbon Burial in Englebright Lake, CA over the Last Century
C. Pondell (2014)
10.1016/J.SOILBIO.2018.03.009
Responses of soil microbial community to continuous experimental nitrogen additions for 13 years in a nitrogen-rich tropical forest
Cong Wang (2018)
Regulation potential of earthworms as related to diversity and functioning of soil microbial community
A. Koubová (2016)
10.1016/j.scitotenv.2010.09.011
Response of soil microbial communities to fire and fire-fighting chemicals.
A. Barreiro (2010)
10.1016/J.SOILBIO.2017.10.036
A comparison of the ability of PLFA and 16S rRNA gene metabarcoding to resolve soil community change and predict ecosystem functions
K. Orwin (2018)
10.1614/WS-D-10-00142.1
Weed Seed Persistence and Microbial Abundance in Long-Term Organic and Conventional Cropping Systems
S. Ullrich (2011)
10.1111/j.1365-2672.2005.02714.x
Fatty acid analysis and spoilage potential of biofilms from two breweries
M. Timke (2005)
10.1016/J.SOILBIO.2010.08.010
Rhizosphere priming of soil organic matter by bacterial groups in a grassland soil
J. Bird (2011)
10.1038/srep27468
Mangrove succession enriches the sediment microbial community in South China
Q. Chen (2016)
10.1016/J.APSOIL.2013.01.005
Responses of soil microbial biomass and community composition to biological soil crusts in the revegetated areas of the Tengger Desert
Yongding Liu (2013)
10.1016/J.SOILBIO.2010.01.020
Factors affecting soil microbial community structure in tomato cropping systems
J. Buyer (2010)
10.1016/J.SOILBIO.2013.09.031
Rapid soil fungal community response to intensive management in a bamboo forest developed from rice paddies
H. Qin (2014)
10.1016/J.SOILBIO.2016.10.004
Mineralisation and sorption of dissolved organic nitrogen compounds in litter and soil from sugarcane fields
E. Mariano (2016)
10.1016/J.SOILBIO.2010.04.007
Microbial populations involved in the suppression of Rhizoctonia solani AG1-1B by lignin incorporation in soil.
Sarah Van Beneden (2010)
10.1016/J.SOILBIO.2014.01.003
Thermal acclimation of organic matter decomposition in an artificial forest soil is related to shifts in microbial community structure
H. Wei (2014)
10.7745/KJSSF.2016.49.2.144
An Overview of Different Techniques on the Microbial Community Structure, and Functional Diversity of Plant Growth Promoting Bacteria
Kiyoon Kim (2016)
10.1089/EES.2010.0015
Speciation of Cadmium and Changes in Bacterial Communities in Red Soil Following Application of Cadmium-Polluted Compost
G. Chen (2010)
10.1007/s11356-012-1061-3
Alterations of microbial populations and composition in the rhizosphere and bulk soil as affected by residual acetochlor
Zhen Long Bai (2012)
10.1021/BK-2014-1158.CH004
Genetic Identification of Fungi Involved in Wood Decay
Grant T Kirker (2014)
10.1007/s10967-019-06834-w
Sources of carbon isotopes in Baltic Sea sediments
G. Lujaniene (2019)
10.1016/j.talanta.2016.07.006
A modified weighted mixture model for the interpretation of spatial and temporal changes in the microbial communities in drinking water reservoirs using compositional phospholipid fatty acid data.
Ivana Stanimirova (2016)
10.1007/s11356-020-08840-y
Co-presence of the anionic surfactant sodium lauryl ether sulphate and the pesticide chlorpyrifos and effects on a natural soil microbial community
Tanita Pescatore (2020)
10.1080/01490451.2015.1025317
New Undescribed Lineages of Non-extremophilic Archaea Form a Homogeneous and Dominant Element Within Alpine Moonmilk Microbiomes
Christoph Reitschuler (2015)
10.3390/f11030262
Soil Microbial Biomass and Community Composition Relates to Poplar Genotypes and Environmental Conditions
L. Karliński (2020)
10.1111/gcb.14937
Dark microbial CO2 fixation in temperate forest soils increases with CO2 concentration.
Marie Spohn (2019)
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