Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Effects Of One Bout Of Endurance Exercise On The Expression Of Myogenin In Human Quadriceps Muscle

F. Kadi, F. Johansson, R. Johansson, Mikael Sjöström, J. Henriksson
Published 2004 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
The objective of this study was to investigate the cellular localisation of MyoD and myogenin in human skeletal muscle fibres as well as the possible alterations in the expression of MyoD and myogenin in response to a single bout of endurance exercise at 40% and 75% of maximum oxygen uptake (VO2 max). Twenty-five biopsies (5 per subject) from the vastus lateralis muscle were obtained before exercise, from the exercising leg at 40% and 75% of VO2 max and from the resting leg following these exercise bouts. The tyramide signal amplification-direct and the Vectastain ABC methods using specific monoclonal antibodies were used to determine the exact location of myogenin and MyoD, to identify muscle satellite cells and to determine myosin heavy chain (MyHC) composition. At rest, myonuclei did not express MyoD or myogenin. Following a single bout of exercise at 40% and 75% of VO2 max, an accumulation of myogenin in myonuclei and not in satellite cells was observed in biopsies from the exercised leg but not in biopsies before exercise and from the resting leg. The number of myogenin-positive myonuclei varied among individuals indicating differences in the response to a single exercise bout. In conclusion, this immunohistochemical study showed that a rapid rearrangement of myogenin expression occurs in exercised human skeletal muscles in response to a single bout of exercise.
This paper references
Denervation induces a rapid nuclear accumulation of MRF4 in mature myofibers
J. Weis (2000)
Expression of myogenic regulatory proteins (myogenin and MyoD1) in small blue round cell tumors of childhood.
N. Wang (1995)
Paediatric rhabdomyosarcoma: MyoD1 demonstration in routinely processed tissue sections using wet heat pretreatment (pressure cooking) for antigen retrieval.
M. Engel (1997)
Satellite cell proliferation and the expression of myogenin and desmin in regenerating skeletal muscle: evidence for two different populations of satellite cells.
Juha Rantanen (1995)
MRF4, Myf-5, and myogenin mRNAs in the adaptive responses of mature rat muscle.
J. Jacobs-El (1995)
Lymphocyte antigen Leu-19 as a molecular marker of regeneration in human skeletal muscle.
W. Schubert (1989)
Quantification of MyoD, myogenin, MRF4 and Id-1 by reverse-transcriptase polymerase chain reaction in rat muscles--effects of hypothyroidism and chronic low-frequency stimulation.
B. Kraus (1997)
Early changes in muscle fiber size and gene expression in response to spinal cord transection and exercise.
E. Dupont-Versteegden (1998)
Selective accumulation of MyoD and myogenin mRNAs in fast and slow adult skeletal muscle is controlled by innervation and hormones.
S. Hughes (1993)
Cellular adaptation of the trapezius muscle in strength-trained athletes
F. Kadi (1999)
Stretch-induced myogenin, MyoD, and MRF4 expression and acute hypertrophy in quail slow-tonic muscle are not dependent upon satellite cell proliferation
D. Lowe (1999)
Effects of endurance training on satellite cell frequency in skeletal muscle of old men. Muscle Nerve
N Charifi (2003)
Identification of skeletal muscle precursor cells in vivo by use of MyoD1 and myogenin probes
M. Grounds (2004)
Hypertrophy-stimulated myogenic regulatory factor mRNA increases are attenuated in fast muscle of aged quails.
D. Lowe (1998)
MRF4 protein expression in regenerating rat muscle
Z. Zhou (2004)
Structural changes in male trapezius muscle with work-related myalgia
F. Kadi (1998)
The expression of the myogenic regulatory factors in denervated and normal muscles of different phenotypes
E. H. Walters (2004)
A myogenic regulatory gene, qmf1, is expressed by adult myonuclei after injury.
Z. Eppley (1993)
MyoD protein accumulates in satellite cells and is neurally regulated in regenerating myotubes and skeletal muscle fibers
K. Koishi (1995)
Determination of muscle contractile properties: the importance of the nerve.
K. Gundersen (1998)
bHLH transcription factor MyoD affects myosin heavy chain expression pattern in a muscle-specific fashion.
D. J. Seward (2001)
Defining the regulatory networks for muscle development.
J. Molkentin (1996)
The MyoD family of myogenic factors is regulated by electrical activity: isolation and characterization of a mouse Myf-5 cDNA.
A. Buonanno (1992)
Three slow myosin heavy chains sequentially expressed in developing mammalian skeletal muscle.
S. Hughes (1993)
Myogenin Induces a Shift of Enzyme Activity from Glycolytic to Oxidative Metabolism in Muscles of Transgenic Mice
S. Hughes (1999)
Myogenin mRNA is elevated during rapid, slow, and maintenance phases of stretch-induced hypertrophy in chicken slow-tonic muscle
J. Carson (1998)
MyoD and myogenin are coexpressed in regenerating skeletal muscle of the mouse
E. M. Füchtbauer (1992)
Cyclic amplification and selection of targets (CASTing) for the myogenin consensus binding site.
W. Wright (1991)
MyoD and myogenin protein expression in skeletal muscles of senile rats
E. Dedkov (2003)
Differential Expression of αB-Crystallin and Hsp27 in Skeletal Muscle during Continuous Contractile Activity
P. Neufer (1996)
Monoclonal antimyogenin antibodies define epitopes outside the bHLH domain where binding interferes with protein-protein and protein-DNA interactions.
W. Wright (1996)
Myogenin and MyoD join a family of skeletal muscle genes regulated by electrical activity.
R. Eftimie (1991)
Differential expression of muscle regulatory factor genes in normal and denervated adult rat hindlimb muscles
S. Voytik (1993)
Adaptation of human skeletal muscle to training and anabolic steroids.
F. Kadi (2000)
Effects of endurance training on satellite cell frequency in skeletal muscle of old men
N. Charifi (2003)
Fibroblast Growth Factor Promotes Recruitment of Skeletal Muscle Satellite Cells in Young and Old Rats
Z. Yablonka-Reuveni (1999)
A gene with homology to myogenin is expressed in developing myotomal musculature of the rainbow trout and in vitro during the conversion of myosatellite cells to myotubes
P. Rescan (1995)
Comparison of the muscle fiber diameter and satellite cell frequency in human muscle biopsies
F. Maier (1999)
MyoD, myogenin independent differentiation of primordial myoblasts in mouse somites
M. G. Cusella-De Angelis (1992)
Oral creatine supplementation facilitates the rehabilitation of disuse atrophy and alters the expression of muscle myogenic factors in humans
P. Hespel (2001)
Expression of myogenic regulatory proteins
NP Wang (1995)
Regenerative potential of human skeletal muscle during aging
V. Renault (2002)
Monoclonal antibodies to the myogenic regulatory protein MyoD1: epitope mapping and diagnostic utility.
P. Dias (1992)
MyoD protein is differentially accumulated in fast and slow skeletal muscle fibres and required for normal fibre type balance in rodents
S. Hughes (1997)
Training effects on the contractile apparatus.
D. Pette (1998)

This paper is referenced by
The impact of sarcopenia and exercise training on skeletal muscle satellite cells
T. Snijders (2009)
Time course of myogenic and metabolic gene expression in response to acute exercise in human skeletal muscle.
Y. Yang (2005)
Co‐expression of IGF‐1 family members with myogenic regulatory factors following acute damaging muscle‐lengthening contractions in humans
Bryon R. McKay (2008)
Satellite cell heterogeneity with respect to expression of MyoD, myogenin, Dlk1 and c-Met in human skeletal muscle: application to a cohort of power lifters and sedentary men
M. Lindström (2010)
Facilitating Exercise Engagement among Community Dwelling Stroke Survivors: Is a once Per Week Group Session Sufficient?
N. A. Mohd Nordin (2019)
Low Intensity Exercise Training Improves Skeletal Muscle Regeneration Potential
T. Pietrangelo (2015)
Skeletal muscle wasting with disuse atrophy is multi-dimensional: the response and interaction of myonuclei, satellite cells and signaling pathways
N. Brooks (2014)
Transcriptional profiling of tissue plasticity: role of shifts in gene expression and technical limitations.
M. Flück (2005)
Skeletal Muscle Morphology and Aerobic Capacity in Patients with Obstructive Sleep Apnoea Syndrome
Britta Wåhlin Larsson (2008)
Identification and characterisation of novel genes involved in skeletal muscle hypertrophy
K. A. Carey (2004)
muscle to acute bouts of resistance exercise Time course of molecular responses of human skeletal
Gregory R. Adams (2015)
Network biology approaches reveal a link between ribosome biogenesis and metabolic reprogramming in ageing skeletal muscles
Kim Clarke (2014)
Frontiers in Skeletal Muscle Wasting, Regeneration and Stem Cells
Carlos Hermano J. Pinheiro (2016)
Predicting tissue-specific enhancers in the human genome.
L. Pennacchio (2007)
Skeletal muscle repair after micro-damage : effect of ice therapy on satellite cell activation
Karen Van Tubbergh (2005)
Influence of preexercise muscle glycogen content on transcriptional activity of metabolic and myogenic genes in well-trained humans.
E. G. Churchley (2007)
Efficacy of 3 days/wk resistance training on myofiber hypertrophy and myogenic mechanisms in young vs. older adults.
David J. Kosek (2006)
Molecular and hormonal responses and adaptation to resistance and protein nutrition in young and older men
Juha J Hulmi (2009)
Satellite Cell Proliferation in Response to a Chronic Laboratory- Controlled Uphill vs. Downhill Interval Training Intervention
Gabriel Eksteen (2006)
Structural Protein Alterations to Resistance and Endurance Cycling Exercise Training
A. Parcell (2009)
Exercise rejuvenates quiescent skeletal muscle stem cells in old mice through restoration of Cyclin D1
Jamie O. Brett (2020)
Role of nitric oxide in muscle regeneration following eccentric muscle contractions in rat skeletal muscle
T. Sakurai (2013)
Skeletal Muscle Morphology in Patients with Restless Legs Syndrome
Britta Wåhlin Larsson (2007)
News and views in Histochemistry and Cell Biology
E. Asan (2004)
Satellite Cell Behavior in Cyclists Following Intensified Training With and Without Protein Supplementation
N. Luden (2016)
Satellite cells in human skeletal muscle : molecular identification quantification and function
M. Lindström (2009)
Nourshahi Maryam (2017)
Neuromuscular electrical stimulation improves skeletal muscle regeneration through satellite cell fusion with myofibers in healthy elderly subjects.
E. D. Di Filippo (2017)
Recent progress in histochemistry and cell biology: the state of the art 2005
D. Taatjes (2005)
Mega roles of microRNAs in regulation of skeletal muscle health and disease
M. Sharma (2014)
Myogenin (Myf4) upregulation in trans-differentiating fibroblasts from a congenital myopathy with arrest of myogenesis and defects of myotube formation
C. Weise (2006)
The behaviour of satellite cells in response to exercise: what have we learned from human studies?
F. Kadi (2005)
See more
Semantic Scholar Logo Some data provided by SemanticScholar