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Molecular Characterization Of Two Plant BI-1 Homologues Which Suppress Bax-induced Apoptosis In Human 293 Cells

N. Bolduc, Mario Ouellet, F. Pitre, L. Brisson
Published 2002 · Biology, Medicine

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Abstract. To date, few homologues of animal programmed cell death (PCD) regulators have been identified in plants. Among these is the plant Bax Inhibitor-1 (BI-1) protein, which possesses, like its human counterpart, the ability to suppress Bax-induced lethality in yeast cells. As the role of BI-1 in the regulation of plant PCD remains to be elucidated, we cloned BnBI-1 and NtBI-1 from cDNA libraries of oilseed rape (Brassica napus L.) and tobacco (Nicotiana tabacum L.). The analysis of the deduced amino acid sequences of BnBI-1 and NtBI-1 indicated that these proteins share a relatively high level of identity with other plant BI-1 proteins (73–95%) as well as with animal BI-1 proteins (26–42%). Comparative analysis with other available plant BI-1 proteins allowed the establishment of a structural model presenting seven transmembrane domains. Moreover, transient co-transfection of Bax with BnBI-1 or NtBI-1 in human embryonic kidney 293 cells revealed that both proteins can substantially inhibit apoptosis induced by Bax overexpression. Localization studies were also conducted using stable transformation of tobacco BY-2 cells and Saccharomyces cerevisiae, or transient expression in tobacco leaves, with the fusion protein BnBI-1GFP under control of the cauliflower mosaic virus 35S promoter. All transformants showed a fluorescence pattern of distribution typical of an endoplasmic reticulum (ER) protein. Results from differential permeabilization experiments in BY-2 cells expressing BnBI-1GFP also showed that the C-terminus is located on the cytosolic side of the ER. Taken altogether, our results suggest that BI-1 is evolutionarily conserved and could act as a key regulator of a death pathway common to plants and animals.
This paper references
10.1128/JVI.76.2.865-874.2002
Intracellular Localization of the Peanut Clump Virus Replication Complex in Tobacco BY-2 Protoplasts Containing Green Fluorescent Protein-Labeled Endoplasmic Reticulum or Golgi Apparatus
P. Dunoyer (2002)
10.1016/0304-4157(95)00003-A
The mitochondrial permeability transition.
M. Zoratti (1995)
10.1093/bioinformatics/12.4.357
TreeView: an application to display phylogenetic trees on personal computers
R. Page (1996)
10.1016/0968-0004(91)90184-W
Nuclear targeting sequences--a consensus?
C. Dingwall (1991)
10.1016/S0167-5699(97)80014-X
[Mitochondrial control of apoptosis].
G. Kroemer (2001)
10.1073/pnas.211423998
Mammalian Bax-induced plant cell death can be down-regulated by overexpression of Arabidopsis Bax Inhibitor-1 (AtBI-1)
M. Kawai-yamada (2001)
10.1016/S0092-8674(00)80564-4
Cell Death in Development
D. Vaux (1999)
10.1046/j.1365-313X.1996.10050935.x
Virus‐mediated delivery of the green fluorescent protein to the endoplasmic reticulum of plant cells
P. Boevink (1996)
10.1126/SCIENCE.291.5507.1279
Apoptotic molecular machinery: vastly increased complexity in vertebrates revealed by genome comparisons.
L. Aravind (2001)
10.1023/A:1026552827716
Regulators of cell death in disease resistance
K. Shirasu (2004)
10.1128/MCB.16.11.6494
Structure-function comparisons of the proapoptotic protein Bax in yeast and mammalian cells.
H. Zha (1996)
10.1073/pnas.93.22.12094
Programmed cell death: a way of life for plants.
J. Greenberg (1996)
10.1099/0022-1317-82-1-29
The open reading frame 1-encoded ('36K') protein of Carnation Italian ringspot virus localizes to mitochondria.
L. Rubino (2001)
10.1055/S-2001-17213
Apoptosis: live or die--hard work either way!
B. Gallaher (2001)
10.1016/S0981-9428(00)01178-5
Plant programmed cell death: a common way to die.
A. Danon (2000)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
A rapid and efficient, nonradioactive method for screening recombinant DNA libraries.
L. Amaravadi (1994)
10.1073/pnas.091108998
Abrogation of disease development in plants expressing animal antiapoptotic genes
M. Dickman (2001)
10.1016/S0014-5793(01)02315-8
Immunocytological localization of two plant fatty acid desaturases in the endoplasmic reticulum
J. Dyer (2001)
10.1016/0092-8674(89)90058-5
KAR2, a karyogamy gene, is the yeast homolog of the mammalian BiP/GRP78 gene
M. Rose (1989)
10.1006/METH.1999.0743
Methods of assaying Bcl-2 and Bax family proteins in yeast.
Q. Xu (1999)
10.1038/sj.cdd.4400365
Bcl-2 family proteins as ion-channels
S. Schendel (1998)
10.1105/tpc.11.4.691
Communicating with Calcium
D. Sanders (1999)
10.1104/PP.010636
Molecular characterization of two Arabidopsis Ire1 homologs, endoplasmic reticulum-located transmembrane protein kinases.
N. Koizumi (2001)
GUS protocols: using the GUS gene as a reporter of gene expression.
S. Gallagher (1992)
10.1105/tpc.12.12.2425
Plant Nuclei Can Contain Extensive Grooves and Invaginations
D. Collings (2000)
The presence/absence of Bcl-2, Ca2+/calmodulin-dependent protein kinase IV, calretinin and p53 in baker's yeast and wheat germ.
W. Kuo (1997)
10.1016/0378-1119(87)90049-7
A family of yeast expression vectors containing the phage f1 intergenic region.
T. Vernet (1987)
A revised medium for the growth and bioassay with tobacco tissue culture
T. Murashige (1962)
10.1046/j.1365-313X.1997.11030613.x
The green fluorescent protein as a marker to visualize plant mitochondria in vivo.
Rainer H. Köhler (1997)
10.1139/o97-056
Detection of a homologue of bcl-2 in plant cells.
M. Dion (1997)
10.1016/S1369-5266(00)00216-8
Regulation and execution of programmed cell death in response to pathogens, stress and developmental cues.
E. Beers (2001)
10.1002/yea.320070902
Structure of the yeast endoplasmic reticulum: localization of ER proteins using immunofluorescence and immunoelectron microscopy.
D. Preuss (1991)
10.1073/PNAS.96.14.7956
Bax-induced cell death in tobacco is similar to the hypersensitive response.
C. Lacomme (1999)
10.1016/S0960-9822(99)80341-8
Animal cell-death suppressors Bcl-xL and Ced-9 inhibit cell death in tobacco plants
I. Mitsuhara (1999)
10.1105/tpc.9.7.1157
Programmed Cell Death in Plants.
R. Pennell (1997)
10.1016/S0014-5793(02)02230-5
Induction of mammalian cell death by a plant Bax inhibitor
L. Yu (2002)
10.1073/pnas.93.2.828
In vivo examination of membrane protein localization and degradation with green fluorescent protein.
R. Hampton (1996)
10.1023/A:1013635427949
Differential expression of putative cell death regulator genes in near-isogenic, resistant and susceptible barley lines during interaction with the powdery mildew fungus
R. Hückelhoven (2004)
10.1016/S1360-1385(00)01605-8
Does the plant mitochondrion integrate cellular stress and regulate programmed cell death?
A. Jones (2000)
10.1042/BJ3410233
The mitochondrial permeability transition pore and its role in cell death.
M. Crompton (1999)
10.1093/NAR/25.24.4876
The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.
J. Thompson (1997)
10.1016/S0014-5793(99)01695-6
Evolutionally conserved plant homologue of the Bax Inhibitor‐1 (BI‐1) gene capable of suppressing Bax‐induced cell death in yeast 1
M. Kawai (1999)
10.1023/A:1026540524990
Regulation of cell death in flower petals
B. Rubinstein (2004)
10.1016/S1097-2765(00)80034-9
Bax inhibitor-1, a mammalian apoptosis suppressor identified by functional screening in yeast.
Q. Xu (1998)
10.1046/J.1365-313X.2000.00690.X
AtBI-1, a plant homologue of Bax inhibitor-1, suppresses Bax-induced cell death in yeast and is rapidly upregulated during wounding and pathogen challenge.
P. Sánchez (2000)
10.1016/S0005-2728(98)00109-1
Mitochondrial control of apoptosis: the role of cytochrome c.
J. Cai (1998)
10.1046/J.1365-313X.1997.11061325.X
An Arabidopsis thaliana cDNA complementing a hamster apoptosis suppressor mutant.
P. Gallois (1997)



This paper is referenced by
10.1038/nrm1358
Controlled cell death, plant survival and development
Eric Lam (2004)
10.1371/journal.pone.0078471
Transcriptome-Wide Mapping of Pea Seed Ageing Reveals a Pivotal Role for Genes Related to Oxidative Stress and Programmed Cell Death
H. Chen (2013)
10.5511/PLANTBIOTECHNOLOGY.22.419
Oxidative stress and plant cell death suppressors
M. Kawai-Yamada (2005)
10.1093/jxb/ers122
Over-expression of Arabidopsis Bax inhibitor-1 delays methyl jasmonate-induced leaf senescence by suppressing the activation of MAP kinase 6.
Haiyun Yue (2012)
10.1371/journal.pone.0020882
Yeast Bax Inhibitor, Bxi1p, Is an ER-Localized Protein That Links the Unfolded Protein Response and Programmed Cell Death in Saccharomyces cerevisiae
James Cebulski (2011)
10.1074/jbc.M109.056648
Hepatic Bax Inhibitor-1 Inhibits IRE1α and Protects from Obesity-associated Insulin Resistance and Glucose Intolerance*
B. Bailly-Maître (2009)
10.1007/1-4020-2217-4_5
Programmed Cell Death in Plants During Development and Stress Responses
S. Panter (2004)
10.1016/J.PMPP.2010.06.001
Transgenic expression of an insect inhibitor of apoptosis gene, SfIAP, confers abiotic and biotic stress tolerance and delays tomato fruit ripening
Wei Li (2010)
10.2174/1566524014666140603101113
The characteristics of Bax inhibitor-1 and its related diseases.
Baojie Li (2014)
10.9758/cpn.2012.10.3.155
Effects of Chronic Mild Stress in Female Bax Inhibitor-1-Gene Knockout Mice
Zhi-Yan Sui (2012)
10.1074/jbc.M706659200
BAX Inhibitor-1 Modulates Endoplasmic Reticulum Stress-mediated Programmed Cell Death in Arabidopsis*
N. Watanabe (2008)
10.1016/j.ceca.2010.02.003
Cardiolipin, phosphatidylserine, and BH4 domain of Bcl-2 family regulate Ca2+/H+ antiporter activity of human Bax inhibitor-1.
T. Ahn (2010)
10.1105/tpc.014613
Dissection of Arabidopsis Bax Inhibitor-1 Suppressing Bax–, Hydrogen Peroxide–, and Salicylic Acid–Induced Cell Death Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.014613.
M. Kawai-yamada (2004)
10.1038/cdd.2011.59
Bax inhibitor-1: a highly conserved endoplasmic reticulum-resident cell death suppressor
T. Ishikawa (2011)
10.1016/j.bbamcr.2007.12.013
The pleiotropic effects of heterologous Bax expression in yeast.
C. Khoury (2008)
10.1016/S0002-9440(10)63682-6
Bax inhibitor-1 is overexpressed in prostate cancer and its specific down-regulation by RNA interference leads to cell death in human prostate carcinoma cells.
M. Grzmil (2003)
10.1093/JXB/ERM239
The hypersensitive response; the centenary is upon us but how much do we know?
L. Mur (2008)
10.1111/j.1469-8137.2008.02642.x
The LCB2 subunit of the sphingolip biosynthesis enzyme serine palmitoyltransferase can function as an attenuator of the hypersensitive response and Bax-induced cell death.
Yun-zhe Gan (2009)
10.1111/j.1365-3040.2010.02117.x
Devil inside: does plant programmed cell death involve the endomembrane system?
Jean-Luc Cacas (2010)
10.1016/j.jplph.2009.04.017
A BAX inhibitor-1 gene in Capsicum annuum is induced under various abiotic stresses and endows multi-tolerance in transgenic tobacco.
Mohammad Isbat (2009)
10.1093/jxb/ers140
Wheat BAX inhibitor-1 contributes to wheat resistance to Puccinia striiformis.
X. Wang (2012)
10.1002/jps.23007
Doxorubicin- and daunorubicin-induced regulation of Ca2+ and H+ fluxes through human bax inhibitor-1 reconstituted into membranes.
Chul-Ho Yun (2012)
10.1016/J.GENE.2003.09.011
Evolutionarily conserved cytoprotection provided by Bax Inhibitor-1 homologs from animals, plants, and yeast.
Han-Jung Chae (2003)
10.1385/JMN:29:1:1
Bax inhibitor-1 protects neurons from oxygen-glucose deprivation
Christoph P. Dohm (2007)
10.7150/IJBS.3.471
The Growth-hormone inducible transmembrane protein (Ghitm) belongs to the Bax inhibitory protein-like family
K. Reimers (2007)
10.1016/j.biocel.2009.03.009
Bax inhibitor-1 overexpression reduces the suppressive effect of calcium mobilizing agent on adipogenesis.
Jiahua Yu (2009)
10.1074/jbc.M304468200
Ultraviolet-C Overexposure Induces Programmed Cell Death in Arabidopsis, Which Is Mediated by Caspase-like Activities and Which Can Be Suppressed by Caspase Inhibitors, p35 and Defender against Apoptotic Death*
A. Danon (2004)
10.1104/pp.106.090878
Cell Death Suppressor Arabidopsis Bax Inhibitor-1 Is Associated with Calmodulin Binding and Ion Homeostasis1[OA]
Yuri Ihara-Ohori (2006)
10.1016/S0074-7696(04)33004-4
Apoptosis in plants: specific features of plant apoptotic cells and effect of various factors and agents.
B. Vanyushin (2004)
10.1371/journal.pone.0179782
An essential role for the VASt domain of the Arabidopsis VAD1 protein in the regulation of defense and cell death in response to pathogens
Mehdi Khafif (2017)
10.1016/j.fsi.2008.11.005
Identification of differentially expressed genes in haemocytes of the crayfish (Procambarus clarkii) infected with white spot syndrome virus by suppression subtractive hybridization and cDNA microarrays.
Yong Zeng (2009)
10.1007/s12892-018-0089-0
Comparative Studies on the Role of Organic Biostimulant in Resistant and Susceptible Cultivars of Rice Grown under Saline Stress - Organic Biostimulant Alleviate Saline Stress in Tolerant and Susceptible Cultivars of Rice
Mohd Shahanbaj Khan (2018)
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