← Back to Search
Development Of Agrobacterium Tumefaciens C58-induced Plant Tumors And Impact On Host Shoots Are Controlled By A Cascade Of Jasmonic Acid, Auxin, Cytokinin, Ethylene And Abscisic Acid
D. Veselov, M. Langhans, W. Hartung, R. Aloni, I. Feussner, C. Götz, S. Veselova, S. Schlomski, C. Dickler, K. Baechmann, Cornelia I. Ullrich
Published 2002 · Biology, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
Abstract. The development of Agrobacterium tumefaciens-induced plant tumors primarily depends on the excessive production of auxin and cytokinin by enzymes encoded on T-DNA genes integrated into the plant genome. The aim of the present study was to investigate the involvement of additional phytohormone signals in the vascularization required for rapid tumor proliferation. In stem tumors of Ricinus communis L., free auxin and zeatin riboside concentrations increased within 2 weeks to 15-fold the concentrations in control stem tissue. Auxin and cytokinin immunolocalization revealed the highest concentrations within and around tumor vascular bundles with concentration gradients. The time-course of changes in free auxin concentration in roots was inversely correlated with that in the tumors. The high ethylene emission induced by increased auxin- and cytokinin correlated with a 36-fold accumulation of abscisic acid in tumors. Ethylene emitted from tumors and exogenously applied ethylene caused an increase in abscisic acid concentrations also in the host leaves, with a diminution in leaf water vapor conductance. Jasmonic acid concentration reached a maximum already within the first week of bacterial infection. A wound effect could be excluded. The results demonstrate the concerted interaction of a cascade of transiently induced, non-T-DNA-encoded phytohormones jasmonic acid, ethylene and abscisic acid with T-DNA-encoded auxin and zeatin riboside plus trans-zeatin, all of which are required for successful plant tumor vascularization and development together with inhibition of host plant growth. Electronic Supplementary Material is available if you access this article at http://dx.doi.org/10.1007/00425-002-0883-5.
This paper references
Isolation and characterization of Arabidopsis mutants defective in the induction of ethylene biosynthesis by cytokinin.
J. Vogel (1998)
Structural and Functional Evidence for Xylem‐Mediated Water Transport and High Transpiration in Agrobacterium tumefaciens‐Induced Tumors of Ricinus communis*
U. Schurr (1996)
Jasmonates and octadecanoids: signals in plant stress responses and development.
C. Wasternack (2002)
Radioimmunoassay for the Natural Plant Growth Regulator (−)-Jasmonic Acid
H. Knoefel (1990)
Ethylene production and ACC‐accumulation in Agrobacterium tumefaciens‐induced plant tumours and their impact on tumour and host stem structure and function
R. Wächter (1999)
Plant hormones : physiology, biochemistry and molecular biology
P. J. Davies (1995)
Interactions between Abscisic Acid and Ethylene Signaling Cascades
N. Beaudoin (2000)
Inputs to the Active Indole-3-Acetic Acid Pool: De Novo Synthesis, Conjugate Hydrolysis, and Indole-3-Butyric Acid b-Oxidation
B. Bartel (2001)
Modified solvent partitioning scheme providing increased specificity and rapidity of immunoassay for indole‐3‐acetic acid
S. Y. Veselov (1992)
Monoclonal Antibodies to Plant Growth Regulators: III. Zeatinriboside and Dihydrozeatinriboside.
J. Eberle (1986)
Indole-3-acetic acid controls cambial growth in scots pine by positional signaling
Reversible protein phosphorylation regulates jasmonic acid-dependent and -independent wound signal transduction pathways in Arabidopsis thaliana.
E. Rojo (1998)
Vascularization is a general requirement for growth of plant and animal tumours.
C. Ullrich (2000)
Influence of temperature increase on evaportranspiration rate and cytokinin content in wheat seedlings
R. G. Farkhutdinov (2004)
Auxin-induced ethylene triggers abscisic acid biosynthesis and growth inhibition.
H. Hansen (2000)
An enzyme‐immunoassay for cis‐(+)‐abscisic acid
E. Weiler (1982)
Induction of crown galls by Agrobacterium tumefaciens (strain C58) reverses assimilate translocation and accumulation in Kalanchoë daigremontiana
S. Malsy (1992)
Callus and tumor development
T Sachs (1991)
Evidence for high activity of xylem parenchyma and ray cells in the interface of host stem and Agrobacterium tumefaciens-induced tumours of Ricinus communis.
J. Pavlovkin (2002)
Biochemistry and Molecular Biology of Plant Hormones
P. Hooykaas (1999)
Plant Hormone Immunoassays Based on Monoclonal and Polyclonal Antibodies
E. Weiler (1986)
Identification and localization of transformed cells in Agrobacterium tumefaciens-induced plant tumors
Claudia Rezmer (1999)
The three-dimensional structure of vascular tissues in Agrobacterium tumefaciens-induced crown galls and in the host stems of Ricinus communis L.
R. Aloni (2004)
The Genome of the Natural Genetic Engineer Agrobacterium tumefaciens C58
D. W. Wood (2001)
Hormone genes and crown gall disease
E. Weiler (1987)
The lipoxygenase pathway.
I. Feussner (2002)
Regulation of Abscisic Acid Signaling by the Ethylene Response Pathway in Arabidopsis
M. Ghassemian (2000)
Ethylenecytokinin interactions in signal transduction
MA Hall (1999)
Competence of Arabidopsis thaliana genotypes and mutants for Agrobacterium tumefaciens-mediated gene transfer: role of phytohormones.
S. Chateau (2000)
Immunofluorescence Detection of F-actin on Low Melting Point Wax Sections from Plant Tissues
S. Vitha (1997)
In situ localization of endogenous cytokinins during shooty tumor development on Eucalyptus globulus Labill.
A. Azmi (2001)
Immunoenzyme test - system for cytokinin determination
GR Kudoyarova (1990)
The never ripe mutant provides evidence that tumor-induced ethylene controls the morphogenesis of agrobacterium tumefaciens-induced crown galls on tomato stems
An Auxin-Dependent Distal Organizer of Pattern and Polarity in the Arabidopsis Root
S. Sabatini (1999)
The Induction of Vascular Tissues by Auxin and Cytokinin
R. Aloni (1995)
Herbert E. Johnson (2003)
Pattern Formation in Plant Tissues: Callus and tumor development
T. Sachs (1991)
Transfer and function of T-DNA genes from Agrobacterium Ti and Ri plasmids in plants
P. Zambryski (1989)
Ethylene‐triggered abscisic acid: A principle in plant growth regulation?
K. Grossmann (2001)
Immunolocalization of plasma-membrane H+-ATPase and tonoplast-type pyrophosphatase in the plasma membrane of the sieve element-companion cell complex in the stem of Ricinus communis L.
M. Langhans (2001)
Drought-induced changes in physiology and ABA
W Hartung (1991)
Foliar and Axial Aspects of Vascular Differentiation: Hypotheses and Evidence
R. Aloni (2001)
Impact of Agrobacterium tumefaciens-induced stem tumors on NO3− uptake in Ricinus communis
I. Mistrík (2004)
Cytokinin Content and Location in the Leaves of the Wild-type and Transgenic Tobacco Plants
S. Veselov (1999)
Anatomy of tumor development in grape stem tissue inoculated with Agrobacterium tumefaciens biovar 3, strain AG 63
F. Tarbah (1988)
Callus and tumor development. In: Sachs T (ed) Pattern formation in plant tissues
T Sachs (1991)
Fast changes in growth rate and cytokinin content of the shoot following rapid cooling of roots of wheat seedling
G. Kudoyarova (2004)
This paper is referenced by
Expression analysis of the NgORF13 promoter during the development of tobacco genetic tumors.
M. Udagawa (2004)
Auswirkungen von externen Stressbedingungen auf die radialen Wasser und ABAFlüsse und den endogenen ABAGehalt des Wurzelgewebes von Maiskeimlingen (Zea mays L.)
Bayerischen Julius (2004)
Influence of Agrobacterium tumefaciens ipt and Agrobacterium rhizogenes rolC Genes on Spontaneous Tumor Formation and Endogenous Cytokinins Content in Radish (Raphanus sativus) Inbred Lines
I. E. Dodueva (2008)
Biological activity of the tzs gene of nopaline Agrobacterium tumefaciens GV3101 in plant regeneration and genetic transformation.
Zhao-fen Han (2013)
Plant responses to Agrobacterium tumefaciens and crown gall development
J. Gohlke (2014)
Nutrients Accumulation and Vascularization of Agrobacterium Tumours Induced on Launaea capitata Spreng. Plant
A. E. Hussein (2014)
Reduction of polar auxin transport in tobacco by the tumorigenic Agrobacterium tumefaciens AK-6b gene
Yasutaka Kakiuchi (2005)
Changes in distribution of zeatin and indole-3-acetic acid in cells during callus induction and organogenesis in vitro in immature embryo culture of wheat
O. A. Seldimirova (2016)
Tumour development in Arabidopsis thaliana involves the Shaker-like K+ channels AKT1 and AKT2/3.
R. Deeken (2003)
Meristematic characteristics of tumors initiated by Agrobacterium tumefaciens in pea plants
A. P. Vinogradova (2015)
Ustilago maydis Produces Cytokinins and Abscisic Acid for Potential Regulation of Tumor Formation in Maize
Stacey A. Bruce (2010)
Bacteria in Agrobiology: Stress Management
D. Maheshwari (2012)
Agrobacteria Enhance Plant Defense Against Root-Knot Nematodes on Tomato.
J. Lamovšek (2017)
Auxin’s role as an example of the mechanisms of shoot/root relations
T. Sachs (2004)
Prevention of plant crown gall tumor development by the anti-malarial artesunate of Artemisia annua
C. Ullrich (2009)
zweier neuer Purintransporter-Familien
Molekulare Charakterisierung (2007)
An Integrated View of Gene Expression and Solute Profiles of Arabidopsis Tumors: A Genome-Wide Approach[W]
R. Deeken (2006)
Comparison between tumors in plants and human beings: Mechanisms of tumor development and therapy with secondary plant metabolites.
C. Ullrich (2019)
The Use of ACC Deaminase to Increase the Tolerance of Plants to Various Phytopathogens
L. Chernin (2012)
Agrobacterium tumefaciens Promotes Tumor Induction by Modulating Pathogen Defense in Arabidopsis thaliana[W]
Chil-Woo Lee (2009)
Radial transport of water and abscisic acid (ABA) in roots of Zea mays under conditions of nutrient deficiency.
Daniela Schraut (2005)
Role of auxin in regulating Arabidopsis flower development
R. Aloni (2005)
The Role of Auxin in Root-Symbiont and Root-Pathogen Interactions: From Development to Defense
U. Mathesius (2010)
Potencies of the resistant maize genotypes against biotic stresses and understanding their strategies
A. Ankala (2010)
Cytotoxic effects of kinetin riboside on mouse, human and plant tumour cells.
B. Griffaut (2004)
Regulation of Agrobacterial Oncogene Expression in Host Plants
Y. Zhang (2014)
Functions and regulation of quorum-sensing in Agrobacterium tumefaciens
J. Lang (2014)
Modulation of Plant Defenses by Ethylene
B. Adie (2007)
Role of reactive oxygen species in auxin herbicide phytotoxicity: current information and hormonal implications — are gibberellins, cytokinins, and polyamines involved?
I. McCarthy-Suárez (2017)
Immunolocalization of IAA and ABA in roots and needles of radiata pine (Pinus radiata) during drought and rewatering.
N. De Diego (2013)
Initiation of spontaneous tumors in radish (Raphanus sativus): Cellular, molecular and physiological events.
Maria A. Lebedeva (Osipova) (2015)
Running title: The role of ABA in crown gall development
R. Deeken (2007)See more