Referencing for people who value simplicity, privacy, and speed.Get Citationsy
← Back to Search
Evidence For The Involvement Of Cell Wall Peroxidase In The Generation Of Hydroxyl Radicals Mediating Extension Growth
A. Liszkay, Barbara Kenk, P. Schopfer
Published 2003 · Chemistry, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy Visualize in Litmaps
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Hydroxyl radicals (·OH), produced in the cell wall, are capable of cleaving wall polymers and can thus mediate cell wall loosening and extension growth. It has recently been proposed that the biochemical mechanism responsible for ·OH generation in the cell walls of growing plant organs represents an enzymatic reaction catalyzed by apoplastic peroxidase (POD). This hypothesis was investigated by supplying cell walls of maize (Zea mays L.) coleoptiles and sunflower (Helianthus annuus L.) hypocotyls with external NADH, an artificial substrate known to cause ·OH generation by POD in vitro. The effects of NADH on wall loosening, growth, and ·OH production in vivo were determined. NADH mediates cell wall extension in vitro and in vivo in an H2O2-dependent reaction that shows the characteristic features of POD. NADH-mediated production of ·OH in vivo was demonstrated in maize coleoptiles using electron paramagnetic resonance spectroscopy in combination with a specific spin-trapping reaction. Kinetic properties and inhibitor/activator sensitivities of the ·OH-producing reaction in the cell walls of coleoptiles resembled the properties of horseradish POD. Apoplastic consumption of external NADH by living coleoptiles can be traced back to the superimposed action of two enzymatic reactions, a KCN-sensitive reaction mediated by POD operating in the ·OH-forming mode, and a KCN-insensitive reaction with the kinetic properties of a superoxide-producing plasma-membrane NADH oxidase the activity of which can be promoted by auxin. Under natural conditions, i.e. in the absence of external NADH, this enzyme may provide superoxide (O2·−) (and H2O2 utilized by POD for) ·OH production in the cell wall.
This paper references
Spin trapping: ESR parameters of spin adducts.
C. Dubose (1988)
Generation of superoxide anion and hydrogen peroxide at the surface of plant cells
A. Vianello (1991)
NADH-stimulated, cyanide-resistant superoxide production in maize coleoptiles analyzed with a tetrazolium-based assay
G. Frahry (2001)
Hydroxyl radical-induced cell-wall loosening in vitro and in vivo: implications for the control of elongation growth.
P. Schopfer (2001)
Oxidation of NADH by hypocotyl segments from soybean is stimulated by 2,4-D.
R. Barr (2000)
NADPH oxidation catalyzed by the peroxidase/H2O2 system. Iodide-mediated oxidation of NADPH to iodinated NADP.
A. Virion (1985)
NADH oxidase activity present on both the external and internal surfaces of soybean plasma membranes
T. DeHahn (1997)
The importance of sodium pyruvate in assessing damage produced by hydrogen peroxide.
A. Giandomenico (1997)
Retardation and inhibition of the cation-induced superoxide generation in BY-2 tobacco cell suspension culture by Zn2+ and Mn2+
Tomonori Kawano (2002)
Characterization of a superoxide dismutase mimic prepared from desferrioxamine and MnO2.
W. Beyer (1989)
Polysaccharide degradation by Fenton reaction--or peroxidase-generated hydroxyl radicals in isolated plant cell walls.
C. Schweikert (2002)
Peroxidases of plasma membranes, apoplastic ascorbate, and relation of redox activities to plant pathology
C Penel (1991)
Wall structure and wall loosening. A look backwards and forwards.
D. Cosgrove (2001)
Scission of polysaccharides by peroxidase-generated hydroxyl radicals.
C. Schweikert (2000)
Enhancement by Ca2+ or Mg2+ of catalytic activity of the superoxide-producing NADPH oxidase in membrane fractions of human neutrophils and monocytes.
H. Suzuki (1985)
1991)MechanismofNADPHoxidation catalyzed by horse-radish peroxidase and 2,4-diacetyl-[2H]heme-substituted horse-radish peroxidase
V DeSandro (1991)
Mechanism of NADPH oxidation catalyzed by horse-radish peroxidase and 2,4-diacetyl-[2H]heme-substituted horse-radish peroxidase.
V. de Sandro (1991)
Determination of Auxin-Dependent pH Changes in Coleoptile Cell Walls by a Null-Point Method
P. Schopfer (1993)
Oxidation states of peroxidase
I. Yamazaki (2005)
NAD(P)H oxidation elicits anion superoxide formation in radish plasmalemma vesicles.
A. Vianello (1989)
Reaction of peroxidase with reduced nicotinamide-adenine dinucleotide and reduced nicotinamide-adenine dinucleotide phosphate.
K. Yokota (1965)
Responses of corn root protoplasts to exogenous reduced nicotinamide adenine dinucleotide: Oxygen consumption, ion uptake, and membrane potential.
W. Lin (1982)
Free radicals in biology and medicine
B. Halliwell (1985)
Physical extensibility of maize coleoptile cell walls: apparent plastic extensibility is due to elastic hysteresis
M. Hohl (2004)
A metalloporphyrin superoxide dismutase mimetic protects against paraquat-induced lung injury in vivo.
B. Day (1996)
Hydroxyl-radical production in physiological reactions. A novel function of peroxidase.
S. X. Chen (1999)
Evidence that hydroxyl radicals mediate auxin-induced extension growth
P. Schopfer (2001)
MechanismofNADPHoxidation catalyzed by horseradish peroxidase and 2 , 4diacetyl[ 2 H ] hemesubstituted horseradish peroxidase
V DeSandro (1991)
Oxidative scission of plant cell wall polysaccharides by ascorbate-induced hydroxyl radicals.
S. Fry (1998)
Lignin synthesis: The generation of hydrogen peroxide and superoxide by horseradish peroxidase and its stimulation by manganese (II) and phenols
B. Halliwell (2004)
Spin Trapping: ESR parameters of spin adducts 1474 1528V
G. Buettner (1987)
 Spin trapping of superoxide and hydroxyl radicals
G. Rosen (1984)
NADPH oxidation catalyzed by the peroxidase/H2O2 system. Guaiacol-mediated and scopoletin-mediated oxidation of NADPH to NADPH+.
J. Michot (1985)
Manganic porphyrins possess catalase activity and protect endothelial cells against hydrogen peroxide-mediated injury.
B. Day (1997)
ASSAY OF CATALASES AND PEROXIDASES, IN METHODS IN ENZYMOLOGY
B. Chance (1995)
Spin trapping evidence for myeloperoxidase-dependent hydroxyl radical formation by human neutrophils and monocytes.
Carroll L. Ramos (1992)
Oxidation of NADH by intact segments of soybean hypocotyls and stimulation by 2,4-D.
C. Hicks (1998)
Spin trapping of superoxide and hydroxyl radicals.
G. Rosen (1984)
Enhancement by Ca 2 + or Mg 2 + of catalytic activity of the superoxideproducing NADPH oxidase in membrane fractions of human neutrophils and monocytes
H Suzuki (1985)
Evidence against the acid-growth theory of auxin action
U. Kutschera (2004)
The oxidation of reduced pyridine nucleotides by peroxidase.
T. Akazawa (1958)
This paper is referenced by
The activity of antioxidative enzymes, contents of H2O2 and of ascorbate in tomato leaves of cultivars more or less sensitive to infection with Botrytis cinerea
J. Patykowski (2005)
Oxidative reactions in axenically seedling roots of olive (Olea europaea, L.)
I. Garrido (2012)
Integration of Pseudomonas fluorescens and salicylic acid improves citrus canker disease management caused by Xanthomonas citri subsp citri-A*
M. Al-Saleh (2015)
Plant glutathione peroxidases: emerging role of the antioxidant enzymes in plant development and stress responses.
Krisztina Bela (2015)
Spin-trapping of oxygen free radicals in chemical and biological systems: new traps, radicals and possibilities.
G. Bačić (2008)
SELENIUM INCREASED THE EFFICIENCY OF ANTIOXIDANT SYSTEM IN ROOT CELLS OF TWO WHEAT CULTIVARS DIFFERING IN ALUMINIUM TOLERANCE
A. Tammam (2012)
Induction of peroxidase isoforms in the roots of two Verbascum thapsus L . populations is involved in adaptive responses to excess Zn 2 + and Cu 2 +
F. Morina (2015)
Interference of Oxidative Metabolism in Citrus by Xanthomonas citri pv citri
R. Ebel (2012)
Impact of UV light on the plant cell wall, methane emissions and ROS production
David J. Messenger (2009)
Effect of stationary magnetic field strengths of 150 and 200 mT on reactive oxygen species production in soybean.
M. Shine (2012)
Zinc induced phytotoxicity mechanism involved in root growth of Triticum aestivum L.
Xiaoning Li (2012)
Oxygen activation at the plasma membrane: relation between superoxide and hydroxyl radical production by isolated membranes
Eiri Heyno (2011)
Active oxygen species and antioxidants in seed biology
C. Bailly (2004)
Different Survival Strategies Amongst Plants to Cope with Underwater Conditions
H. V. Veen (2014)
Transcriptional landscape of soybean (Glycine max) embryonic axes during germination in the presence of paclobutrazol, a gibberellin biosynthesis inhibitor
Rajesh Kumar Gazara (2019)
A Defense Associated Peroxidase from Lemon Having Dye DecolorizingAbility and Offering Resistance to Heat, Heavy Metals and Organic Solvents
P. VedaP (2016)
Systematic analysis of maize class III peroxidase gene family reveals a conserved subfamily involved in abiotic stress response.
Y. Wang (2015)
Papain-templated Cu nanoclusters: assaying and exhibiting dramatic antibacterial activity cooperating with H₂O₂.
H. Miao (2015)
Regulation of Plasmodesmal Transport and Modification of Plasmodesmata During Development and Following Infection by Viruses and Viral Proteins
Tessa M Burch-Smith (2016)
Legumes display common and host-specific responses to the rhizobial cellulase CelC2 during primary symbiotic infection
E. Menéndez (2019)
The role of xylem class III peroxidases in lignification.
K. Marjamaa (2009)
Specificity of polyamine effects on NaCl-induced ion flux kinetics and salt stress amelioration in plants.
C. Pandolfi (2010)
CENTRO DE ENERGIA NUCLEAR NA AGRICULTURA
L. Borgo (2010)
Aluminum-induced cell death of barley-root border cells is correlated with peroxidase- and oxalate oxidase-mediated hydrogen peroxide production
L. Tamás (2005)
Signaling and Communication in Plants
S. Mugnai (2011)
Interactive effect of static magnetic field and abiotic stressors on growth and biochemical parameters of germinating wheat cultivars
Ayşe Şen (2017)
Powdery mildew susceptibility and biotrophic infection strategies
Justus-Liebig-University Giessen (2005)
Effects of jasmonate and some other signalling factors on bean and onion growth during the initial phase of cadmium action
W. Maksymiec (2011)
Effect of Ca2+ on programmed death of guard and epidermal cells of pea leaves
D. B. Kiselevsky (2010)
The mechanics behind plant development.
O. Hamant (2010)
Pectic polysaccharides are attacked by hydroxyl radicals in ripening fruit: evidence from a fluorescent fingerprinting method
O. Airianah (2016)
Impact of M agnetic F ield on C rop P lants
M. Shine (2016)See more