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Light Conditions Influence Specific Defence Responses In Incompatible Plant–pathogen Interactions: Uncoupling Systemic Resistance From Salicylic Acid And PR-1 Accumulation
J. Zeier, B. Pink, M. Mueller, Susanne Berger
Published 2004 · Medicine, Biology
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In incompatible plant–pathogen interactions, disease resistance is generated by rapid activation of a multitude of plant defence reactions. Little is known about the dependency of these resistance responses on external factors. The plasticity of plant defence mechanisms in terms of light conditions is studied here. Interaction of Arabidopsis thaliana (L.) Heynh. with an avirulent strain of Pseudomonas syringae pv. maculicola in the dark resulted in increased apoplastic bacterial growth and therefore reduced local resistance as compared to an infection process in the presence of light. Several characteristic defence reactions, including activation of phenylalanine ammonia-lyase, accumulation of salicylic acid (SA), expression of the pathogenesis-related protein PR-1 and the development of a microscopically defined hypersensitive response, proved to be light dependent. In contrast, the extent of the oxidative burst, as estimated by induction of the protectant gene glutathione-S-transferase, was not weakened by the absence of light. Moreover, pathogen-induced accumulation of jasmonic acid, production of the phytoalexin camalexin and transcriptional induction of a pathogen-inducible myrosinase were even more pronounced in the dark. Apart from affecting local defence responses, light also influenced the establishment of systemic acquired resistance (SAR). SAR development in response to infection by avirulent bacteria was completely lost when the primary infection process occurred in the absence of light. SAR developed both under medium (70 μmol photons m−2 s−1) and strong (500 μmol photons m−2 s−1) light conditions but was in the latter case not associated with an accumulation of SA and PR-1 in systemic leaves, demonstrating that SAR can be executed independently from these molecular SAR markers. Our results are consistent with the notion that SA accumulation in infected primary leaves is necessary for induction of systemic resistance and indicate that defence mechanisms different from SA signalling and PR-protein action exist in systemic tissue to confer resistance during SAR.
This paper references
Arabidopsis is susceptible to infection by a downy mildew fungus.
E. Koch (1990)
Resistance to Pseudomonas syringae
AP Kloek (2001)
Salicylic Acid Induction–Deficient Mutants of Arabidopsis Express PR-2 and PR-5 and Accumulate High Levels of Camalexin after Pathogen Inoculation
C. Nawrath (1999)
An Arabidopsis thaliana Thionin Gene Is Inducible via a Signal Transduction Pathway Different from That for Pathogenesis-Related Proteins
P. Epple (1995)
Increased tolerance to two oomycete pathogens in transgenic tobacco expressing pathogenesis-related protein 1a.
D. Alexander (1993)
Signaling in Soybean Phenylpropanoid Responses (Dissection of Primary, Secondary, and Conditioning Effects of Light, Wounding, and Elicitor Treatments)
T. L. Graham (1996)
PAD4 Functions Upstream from Salicylic Acid to Control Defense Responses in Arabidopsis
N. Zhou (1998)
Resistance to Pseudomonas syringae conferred by an Arabidopsis thaliana coronatine-insensitive (coi1) mutation occurs through two distinct mechanisms.
A. P. Kloek (2001)
Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley—powdery mildew interaction
H. Thordal-Christensen (1997)
Uncoupling PR Gene Expression from NPR1 and Bacterial Resistance: Characterization of the Dominant Arabidopsis cpr6-1 Mutant
J. Clarke (1998)
Glucosinolate research in the Arabidopsis era.
Ute Wittstock (2002)
Characterization of Arabidopsis enhanced disease susceptibility mutants that are affected in systemically induced resistance.
J. Ton (2002)
Quantification of jasmonic acid by capillary gas chromatography-negative chemical ionization-mass spectrometry.
M. Mueller (1994)
A disease resistance gene in Arabidopsis with specificity for two different pathogen avirulence genes.
S. Bisgrove (1994)
Loss of non-host resistance of Arabidopsis NahG to Pseudomonas syringae pv. phaseolicola is due to degradation products of salicylic acid.
S. V. Van Wees (2003)
Systemic Acquired Resistance.
J. Ryals (1996)
Three unique mutants of Arabidopsis identify eds loci required for limiting growth of a biotrophic fungal pathogen.
J. Dewdney (2000)
Topology of the network integrating salicylate and jasmonate signal transduction derived from global expression phenotyping.
J. Glazebrook (2003)
Salicylic Acid Is Not the Translocated Signal Responsible for Inducing Systemic Acquired Resistance but Is Required in Signal Transduction.
B. Vernooij (1994)
Knockout of Arabidopsis accelerated-cell-death11 encoding a sphingosine transfer protein causes activation of programmed cell death and defense.
P. Brodersen (2002)
Nuclear Localization of NPR1 Is Required for Activation of PR Gene Expression
M. Kinkema (2000)
NPR1 Modulates Cross-Talk between Salicylate- and Jasmonate-Dependent Defense Pathways through a Novel Function in the Cytosol Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.009159.
S. Spoel (2003)
H 2 O 2 from the oxidative burst orchestrates the plant hypersensitive response
J Malamy (1994)
A Central Role of Salicylic Acid in Plant Disease Resistance
T. Delaney (1994)
Isolation of phytoalexin-deficient mutants of Arabidopsis thaliana and characterization of their interactions with bacterial pathogens.
J. Glazebrook (1994)
Signal interactions between nitric oxide and reactive oxygen intermediates in the plant hypersensitive disease resistance response
M. Delledonne (2001)
Inducers of Plant Systemic Acquired Resistance Regulate NPR1 Function through Redox Changes
Zhonglin Mou (2003)
Production of Salicylic Acid Precursors Is a Major Function of Phenylalanine Ammonia-Lyase in the Resistance of Arabidopsis to Peronospora parasitica.
B. Mauch-Mani (1996)
The myrosinase gene family in Arabidopsis thaliana: gene organization, expression and evolution
J. Xue (2004)
Arabidopsis enhanced disease susceptibility mutants exhibit enhanced susceptibility to several bacterial pathogens and alterations in PR-1 gene expression.
E. Rogers (1997)
Requirement of Salicylic Acid for the Induction of Systemic Acquired Resistance
T. Gaffney (1993)
Phytochrome signalling modulates the SA-perceptive pathway in Arabidopsis.
T. Genoud (2002)
Arabidopsis SON1 Is an F-Box Protein That Regulates a Novel Induced Defense Response Independent of Both Salicylic Acid and Systemic Acquired Resistance Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.001867.
H. S. Kim (2002)
Systemic resistance in Arabidopsis induced by biocontrol bacteria is independent of salicylic acid accumulation and pathogenesis-related gene expression.
C. Pieterse (1996)
Salicylic Acid: A Likely Endogenous Signal in the Resistance Response of Tobacco to Viral Infection
J. Malamy (1990)
Jasmonic Acid Accumulation in Tobacco Hypersensitive Response Lesions
P. Kenton (1999)
Jasmonate-dependent induction of indole glucosinolates in Arabidopsis by culture filtrates of the nonspecific pathogen Erwinia carotovora.
G. Brader (2001)
Untersuchungen zur Jasmonat-Signaltransduktion in Arabidopsis thaliana anhand des Jasmonat-regulierten Gens Atjrg21
S. Bau (2001)
Overproduction of salicylic acid in plants by bacterial transgenes enhances pathogen resistance
M. Verberne (2000)
EDS5, an Essential Component of Salicylic Acid–Dependent Signaling for Disease Resistance in Arabidopsis, Is a Member of the MATE Transporter Family Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010376.
C. Nawrath (2002)
Characterization of a glutathione S-transferase gene ATGST 1 in Arabidopsis thaliana
K. Yang (1998)
THE OXIDATIVE BURST IN PLANT DISEASE RESISTANCE.
C. Lamb (1997)
H2O2 from the oxidative burst orchestrates the plant hypersensitive disease resistance response
Alex Levine (1994)
Acetylsalicylic acid (aspirin) induces resistance to tobacco mosaic virus in tobacco.
R. White (1979)
Systemic acquired resistance
Jean-Pierre Métraux (2004)
Isolation of new Arabidopsis mutants with enhanced disease susceptibility to Pseudomonas syringae by direct screening.
S. Volko (1998)
Suppression and Restoration of Lesion Formation in Arabidopsis lsd Mutants.
K. Weymann (1995)
Acquired resistance in Arabidopsis.
S. Uknes (1992)
Jasmonate and salicylate as global signals for defense gene expression.
P. Reymond (1998)
Phytoalexin Accumulation in Arabidopsis thaliana during the Hypersensitive Reaction to Pseudomonas syringae pv syringae.
J. Tsuji (1992)
Inverse relationship between systemic resistance of plants to microorganisms and to insect herbivory
G. Felton (1999)
H Thordal-Christensen (1997)
Salicylic Acid Is Needed in Hypersensitive Cell Death in Soybean but Does Not Act as a Catalase Inhibitor
R. Tenhaken (1997)
Fumonisin B1–Induced Cell Death in Arabidopsis Protoplasts Requires Jasmonate-, Ethylene-, and Salicylate-Dependent Signaling Pathways
T. Asai (2000)
Reactive Oxygen Intermediates Mediate a Systemic Signal Network in the Establishment of Plant Immunity
M. E. Alvarez (1998)
Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis.
S. Karpiński (1999)
Isochorismate synthase is required to synthesize salicylic acid for plant defence
M. Wildermuth (2001)
This paper is referenced by
Go in for the kill
Liang Wu (2014)
Red light delays programmed cell death in non-host interaction between Pseudomonas syringae pv tomato DC3000 and tobacco plants.
Laura Moyano (2020)
Induction of Resistance to Crop Diseases
P. Narayanasamy (2008)
Primary metabolism and plant defense--fuel for the fire.
M. Bolton (2009)
Rice Stress Associated Protein 1 (OsSAP1) Interacts with Aminotransferase (OsAMTR1) and Pathogenesis-Related 1a Protein (OsSCP) and Regulates Abiotic Stress Responses
Kamakshi S. Kothari (2016)
Myrosinase: insights on structural, catalytic, regulatory, and environmental interactions
R. Bhat (2019)
Transcriptional, microscopic and macroscopic investigations into monogenic and polygenic interactions of tomato and powdery mildew
Li Cheng-wei (2005)
A role for b-sitosterol to stigmasterol conversion in plant – pathogen interactions
T. Griebel (2010)
Loss of chloroplast-localized protein phosphatase 2Cs in Arabidopsis thaliana leads to enhancement of plant immunity and resistance to Xanthomonas campestris pv. campestris infection.
Chiharu Akimoto-Tomiyama (2018)
Age-dependent variations of local and systemic defence responses in Arabidopsis leaves towards an avirulent strain of Pseudomonas syringae
J. Zeier (2005)
Unveiling the global patterns of diurnally oscillating genes in a wild tobacco Nicotiana attenuata
W. Walter (2013)
Many Shades of Grey in Botrytis-Host Plant Interactions.
J. Veloso (2018)
Methyl esterase 1 (StMES1) is required for systemic acquired resistance in potato.
Patricia Manosalva (2010)
Fatty Acid Hydroperoxides and H2O2 in the Execution of Hypersensitive Cell Death in Tobacco Leaves1[w]
Jean-Luc Montillet (2005)
Early Transcriptional Defense Responses in Arabidopsis Cell Suspension Culture under High-Light Conditions1[C][W][OA]
S. González-Pérez (2011)
Effector-Triggered Immune Response in Arabidopsis thaliana Is a Quantitative Trait
Michail Iakovidis (2016)
Effect of Artificial Light Conditions on Local and Systemic Resistance Response of Tobacco to TMV Infection
Zoltán Á. Nagy (2017)
Purification of low-abundance Arabidopsis plasma-membrane protein complexes and identification of candidate components.
Yiping Qi (2009)
Light Suppresses Bacterial Population through the Accumulation of Hydrogen Peroxide in Tobacco Leaves Infected with Pseudomonas syringae pv. tabaci
Dan-dan Cheng (2016)
A Versatile Method for Mounting Arabidopsis Leaves for Intravital Time-lapse Imaging.
S. Betsuyaku (2019)
Induced resistance for plant disease control: maximizing the efficacy of resistance elicitors.
D. Walters (2005)
Arabidopsis Di19 functions as a transcription factor and modulates PR1, PR2, and PR5 expression in response to drought stress.
W. Liu (2013)
The immediate wound‐induced oxidative burst of Saccharina latissima depends on light via photosynthetic electron transport
Ruth E. McDowell (2015)
Light-regulated plant growth and development.
C. Kami (2010)
New light shed on life and death: the role of staygreen in the hypersensitive response.
Hilary J Rogers (2010)
Heat Shock Factor HsfB1 Primes Gene Transcription and Systemic Acquired Resistance in Arabidopsis1[W]
T. Pick (2012)
Cross-Talk of Mitochondria and Chloroplasts
Michela Zottini (2013)
Light Influences How the Fungal Toxin Deoxynivalenol Affects Plant Cell Death and Defense Responses
Khairul I. Ansari (2014)
Interaction of Botrytis cinerea with the intermediate C3-CAM plant Mesembryanthemum crystallinum
E. Kuzniak (2010)
Analysis of the defence phosphoproteome of Arabidopsis thaliana using differential mass tagging
A. Jones (2006)
Molecular and functional profiling of Arabidopsis pathogenesis-related genes: insights into their roles in salt response of seed germination.
P. J. Seo (2008)
Glutathione S-Transferase Enzymes in Plant-Pathogen Interactions
G. Gullner (2018)See more