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Antisense Reduction Of Thylakoidal Ascorbate Peroxidase In Arabidopsis Enhances Paraquat-induced Photooxidative Stress And Nitric Oxide-induced Cell Death
D. Tarantino, C. Vannini, M. Bracale, M. Campa, C. Soave, I. Murgia
Published 2005 · Biology, Medicine
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The production and characterization of Arabidopsis plants containing a transgene in which the Arabidopsis tAPX is inserted in antisense orientation, is described. tAPX activity in these transgenic tAPX plants is around 50% of control level. The tAPX antisense plants are phenotypically indistinguishable from control plants under normal growth conditions; they show, however, enhanced sensitivity to the O2−-generating herbicide, Paraquat. Interestingly, the tAPX antisense plants show enhanced symptoms of damage when cell death is triggered through treatment with the nitric oxide-donor, SNP. These results are in accordance with the ones recently obtained with transgenic plants overexpressing tAPX; altogether, they suggest that tAPX, besides the known ROS scavenging role, is also involved in the fine changes of H2O2 concentration during signaling events.
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