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Antisense Reduction Of Thylakoidal Ascorbate Peroxidase In Arabidopsis Enhances Paraquat-induced Photooxidative Stress And Nitric Oxide-induced Cell Death

D. Tarantino, C. Vannini, M. Bracale, M. Campa, C. Soave, I. Murgia
Published 2005 · Biology, Medicine

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The production and characterization of Arabidopsis plants containing a transgene in which the Arabidopsis tAPX is inserted in antisense orientation, is described. tAPX activity in these transgenic tAPX plants is around 50% of control level. The tAPX antisense plants are phenotypically indistinguishable from control plants under normal growth conditions; they show, however, enhanced sensitivity to the O2−-generating herbicide, Paraquat. Interestingly, the tAPX antisense plants show enhanced symptoms of damage when cell death is triggered through treatment with the nitric oxide-donor, SNP. These results are in accordance with the ones recently obtained with transgenic plants overexpressing tAPX; altogether, they suggest that tAPX, besides the known ROS scavenging role, is also involved in the fine changes of H2O2 concentration during signaling events.
This paper references
10.1104/pp.001362
Heat Stress- and Heat Shock Transcription Factor-Dependent Expression and Activity of Ascorbate Peroxidase in Arabidopsis1
I. I. Panchuk (2002)
10.1093/OXFORDJOURNALS.PCP.A029599
Overexpression of an Arabidopsis peroxisomal ascorbate peroxidase gene in tobacco increases protection against oxidative stress.
J. Wang (1999)
Identification of two ascorbate peroxidase cDNAs from soybean leaves and characterization of their products by functional expression in E.coli
CR Caldwell (1998)
10.1016/S0005-2728(89)80347-0
Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy
R. J. Porra (1989)
10.1089/152308603321223522
The functions of nitric oxide-mediated signaling and changes in gene expression during the hypersensitive response.
M. Delledonne (2003)
10.1046/J.1365-313X.1998.00343.X
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.
S. Clough (1998)
10.1046/J.1365-313X.2002.01427.X
Double antisense plants lacking ascorbate peroxidase and catalase are less sensitive to oxidative stress than single antisense plants lacking ascorbate peroxidase or catalase.
L. Rizhsky (2002)
10.1016/S0304-4165(01)00131-3
Characterization of monoclonal antibodies against ascorbate peroxidase isoenzymes: purification and epitope-mapping using immunoaffinity column chromatography.
K. Yoshimura (2001)
10.1093/OXFORDJOURNALS.PCP.A028913
Efficient promoter cassettes for enhanced expression of foreign genes in dicotyledonous and monocotyledonous plants.
I. Mitsuhara (1996)
Enzymes in isolated chloroplasts . Polypheno - loxidase in Beta vulgaris
K Asada (1949)
10.1104/PP.123.1.223
Expression of spinach ascorbate peroxidase isoenzymes in response to oxidative stresses.
K. Yoshimura (2000)
10.1093/JEXBOT/53.372.1305
Regulation and function of ascorbate peroxidase isoenzymes.
S. Shigeoka (2002)
10.1104/pp.103.1.165
A Mutant of Arabidopsis thaliana with a Reduced Response to Fusicoccin. I
S. Gomarasca (1993)
10.1007/s004250050237
Identification of two cytosolic ascorbate peroxidase cDNAs from soybean leaves and characterization of their products by functional expression in E. coli
C. R. Caldwell (1997)
10.1007/0-306-48148-0
Regulation of Photosynthesis
Eva-Mari Aro (2001)
10.1111/J.1365-313X.2004.02092.X
Arabidopsis thaliana plants overexpressing thylakoidal ascorbate peroxidase show increased resistance to Paraquat-induced photooxidative stress and to nitric oxide-induced cell death.
I. Murgia (2004)
Enzymes in isolated chloroplasts
DI Arnon (1949)
10.1007/0-306-48148-0_27
Redox Sensing of Photooxidative Stress and Acclimatory Mechanisms in Plants
S. Karpiński (2001)
10.1016/S0981-9428(00)01188-8
Purification and characterization of an ascorbate peroxidase from potato tuber mitochondria
S. Leonardis (2000)
10.1104/pp.114.1.275
Evidence for the Presence of the Ascorbate-Glutathione Cycle in Mitochondria and Peroxisomes of Pea Leaves
A. Jiménez (1997)
10.1016/S1360-1385(02)02312-9
Oxidative stress, antioxidants and stress tolerance.
R. Mittler (2002)
10.1016/S0378-1119(01)00566-2
Cloning of peroxisomal ascorbate peroxidase gene from barley and enhanced thermotolerance by overexpressing in Arabidopsis thaliana.
W. M. Shi (2001)
10.1073/PNAS.96.24.14165
Transgenic tobacco plants with reduced capability to detoxify reactive oxygen intermediates are hyperresponsive to pathogen infection.
R. Mittler (1999)
10.1046/J.1365-313X.2002.01476.X
Thylakoid membrane-bound ascorbate peroxidase is a limiting factor of antioxidative systems under photo-oxidative stress.
Y. Yabuta (2002)
10.1016/S0981-9428(02)01397-9
Reactive oxygen intermediates modulate nitric oxide signaling in the plant hypersensitive disease-resistance response
M. Delledonne (2002)
10.1016/J.JPLPH.2003.12.004
Comparative effects of various nitric oxide donors on ferritin regulation, programmed cell death, and cell redox state in plant cells.
I. Murgia (2004)
10.1104/pp.114.2.529
Overproduction of Ascorbate Peroxidase in the Tobacco Chloroplast Does Not Provide Protection against Ozone
G. Torsethaugen (1997)
10.1146/ANNUREV.ARPLANT.49.1.249
ASCORBATE AND GLUTATHIONE: Keeping Active Oxygen Under Control.
G. Noctor (1998)
10.1104/pp.107.4.1049
Dissection of Oxidative Stress Tolerance Using Transgenic Plants
R. D. Allen (1995)
10.1023/A:1011818021875
Preparation and functional characterization of thylakoids from Arabidopsis thaliana
A. P. Casazza (2004)
10.1023/A:1006110223774
Signals controlling the expression of cytosolic ascorbate peroxidase during pathogen-induced programmed cell death in tobacco
R. Mittler (2004)
10.1073/pnas.231178298
Signal interactions between nitric oxide and reactive oxygen intermediates in the plant hypersensitive disease resistance response
M. Delledonne (2001)
10.1093/JEXBOT/52.365.2345
Protecting cotton photosynthesis during moderate chilling at high light intensity by increasing chloroplastic antioxidant enzyme activity.
P. Payton (2001)
Nitric oxide-mediated signaling functions and change in genes expression during the plant hypersensitive response
M Delledonne (2003)
10.1007/s00425-003-1038-z
Differential involvement of the IDRS cis-element in the developmental and environmental regulation of the AtFer1 ferritin gene from Arabidopsis
D. Tarantino (2003)
10.1042/BJ3260305
From sequence analysis of three novel ascorbate peroxidases from Arabidopsis thaliana to structure, function and evolution of seven types of ascorbate peroxidase.
H. Jespersen (1997)
10.1104/pp.005629
Changes in the Antioxidant Systems as Part of the Signaling Pathway Responsible for the Programmed Cell Death Activated by Nitric Oxide and Reactive Oxygen Species in Tobacco Bright-Yellow 2 Cells1
M. D. de Pinto (2002)
10.1146/ANNUREV.ARPLANT.50.1.601
THE WATER-WATER CYCLE IN CHLOROPLASTS: Scavenging of Active Oxygens and Dissipation of Excess Photons.
K. Asada (1999)
10.1104/PP.123.3.895
Female reproductive tissues are the primary target of Agrobacterium-mediated transformation by the Arabidopsis floral-dip method.
C. Desfeux (2000)
10.1093/OXFORDJOURNALS.PCP.A028938
Photoinhibition of Photosystem I: Its Physiological Significance in the Chilling Sensitivity of Plants
K. Sonoike (1996)
10.1098/RSTB.2000.0703
The water-water cycle as alternative photon and electron sinks.
K. Asada (2000)
10.1038/29087
Nitric oxide functions as a signal in plant disease resistance
M. Delledonne (1998)
10.1016/S1360-1385(01)01946-X
Abiotic stress signalling pathways: specificity and cross-talk.
Heather Knight (2001)
10.1105/tpc.10.3.461
Post-Transcriptional Suppression of Cytosolic Ascorbate Peroxidase Expression during Pathogen-Induced Programmed Cell Death in Tobacco
R. Mittler (1998)
10.1038/35021067
Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells
Zhen-Ming Pei (2000)
10.1016/S0891-5849(97)00107-X
Use of transgenic plants to study antioxidant defenses.
R. D. Allen (1997)
Superoxide dismutases and stress tolerance
C Bowler (1992)
10.1146/ANNUREV.PP.43.060192.000503
SUPEROXIDE DISMUTASE AND STRESS TOLERANCE
C. Bowler (1992)
10.1104/PP.24.1.1
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.
D. Arnon (1949)



This paper is referenced by
Update on Reactive Nitrogen and Oxygen Species Cross Talk between Reactive Nitrogen and Oxygen Species during the Hypersensitive Disease Resistance Response
Federica Zaninotto (2006)
10.1104/pp.106.079160
Mitogen-Activated Protein Kinases and Reactive Oxygen Species Signaling in Plants1
A. Pitzschke (2006)
Biochemische Charakterisierung und bioinformatische Sequenzanalyse plasmamembrangebundener Peroxidasen aus Maiswurzeln (Zea mays L.)
A. Mika (2006)
10.1016/j.plaphy.2015.05.011
Analysis of Arabidopsis thaliana atfer4-1, atfh and atfer4-1/atfh mutants uncovers frataxin and ferritin contributions to leaf ionome homeostasis.
Irene Murgia (2015)
Update on Reactive Oxygen Species in Chloroplasts Production and Scavenging of Reactive Oxygen Species in Chloroplasts and Their Functions
Kozi Asada (2006)
10.1007/s11816-015-0343-x
H2O2 production and gene expression of antioxidant enzymes in kimchi cabbage (Brassica rapa var. glabra Regel) seedlings regulated by plant development and nitrosative stress-triggered cell death
Y. J. Kim (2015)
10.1111/j.1438-8677.2009.00209.x
Biochemical changes associated with in vivo RbcL fragmentation by reactive oxygen species under chilling-light conditions.
R. Nakano (2010)
10.1111/nph.14214
Molybdenum and iron mutually impact their homeostasis in cucumber (Cucumis sativus) plants.
G. Vigani (2017)
10.1186/s13104-017-2489-9
Difference in oxidative stress tolerance between rice cultivars estimated with chlorophyll fluorescence analysis
Ichiro Kasajima (2017)
10.3109/07388550903524243
Roles of enzymatic and nonenzymatic antioxidants in plants during abiotic stress
P. Ahmad (2010)
10.1016/j.jplph.2012.02.012
Overexpression of thylakoidal ascorbate peroxidase shows enhanced resistance to chilling stress in tomato.
M. Duan (2012)
10.1146/annurev.genet.42.110807.091452
The dynamics of photosynthesis.
S. Eberhard (2008)
CHARACTERIZATION OF FIBRILLIN4 PROTEIN FUNCTION IN
Dharmendra Kumar Singh (2011)
10.1093/JXB/ERJ053
Nitric oxide and gene regulation in plants.
S. Grün (2006)
10.1007/s10535-010-0039-6
Multifunctional genes: the cross-talk among the regulation networks of abiotic stress responses
X. J. Hu (2010)
10.1038/s41598-017-10568-8
Transcriptome and proteome analysis reveal new insight into proximal and distal responses of wheat to foliar infection by Xanthomonas translucens
D. Garcia-Seco (2017)
10.1016/J.PLAPHY.2007.09.007
Knock-out of ferritin AtFer1 causes earlier onset of age-dependent leaf senescence in Arabidopsis.
I. Murgia (2007)
Modulating the H2O2 production in chloroplasts
V. Maurino (2008)
10.1104/pp.106.082040
Production and Scavenging of Reactive Oxygen Species in Chloroplasts and Their Functions1
K. Asada (2006)
10.1104/pp.107.101436
Double Mutants Deficient in Cytosolic and Thylakoid Ascorbate Peroxidase Reveal a Complex Mode of Interaction between Reactive Oxygen Species, Plant Development, and Response to Abiotic Stresses1[W][OA]
G. Miller (2007)
10.3389/fpls.2014.00207
Enhancing crop resilience to combined abiotic and biotic stress through the dissection of physiological and molecular crosstalk
C. Kissoudis (2014)
10.1007/978-3-540-72954-9_9
Oxidative Stress and Salt Tolerance in Plants
Cai-Hong Pang (2008)
10.1007/BF02704112
The reactive oxygen species network pathways: an essential prerequisite for perception of pathogen attack and the acquired disease resistance in plants
S. Kotchoni (2007)
10.1016/j.niox.2014.06.008
Detection and function of nitric oxide during the hypersensitive response in Arabidopsis thaliana: where there's a will there's a way.
J. Chen (2014)
10.5897/AJB11.857
Thylakoid-bound ascorbate peroxidase increases resistance to salt stress and drought in Brassica napus
Jianmei Wang (2011)
10.1016/j.jphotobiol.2014.09.007
Effect of biogenic polyamine spermine on the structure and function of photosystem I.
Hnia YaakoubiH. Yaakoubi (2014)
10.1016/j.abb.2010.03.024
Evolution of structure and function of Class I peroxidases.
M. Zámocký (2010)
10.5897/AJB11.2419
Ascorbate peroxidase gene from Brassica napus enhances salt and drought tolerances in Arabidopsis thaliana
Liang Chai (2011)
10.1016/B978-0-12-385853-5.00003-9
Vitamin C: The Metabolism and Functions of Ascorbic Acid in Plants
N. Smirnoff (2011)
10.1080/09168451.2014.942254
Cellular redox regulation, signaling, and stress response in plants
S. Shigeoka (2014)
10.4161/psb.2.6.4802
Interplay Among Nitric Oxide and Reactive Oxygen Species
J. Zhao (2007)
10.1111/j.1438-8677.2012.00598.x
Hydrogen peroxide is involved in nitric oxide-induced cell death in maize leaves.
X. Kong (2013)
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