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Hydrogen Production By Chlamydomonas Reinhardtii: An Elaborate Interplay Of Electron Sources And Sinks

A. Hemschemeier, S. Fouchard, L. Cournac, G. Peltier, T. Happe
Published 2007 · Biology, Medicine

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The unicellular green alga Chlamydomonas reinhardtii possesses a [FeFe]-hydrogenase HydA1 (EC 1.12.7.2), which is coupled to the photosynthetic electron transport chain. Large amounts of H2 are produced in a light-dependent reaction for several days when C. reinhardtii cells are deprived of sulfur. Under these conditions, the cells drastically change their physiology from aerobic photosynthetic growth to an anaerobic resting state. The understanding of the underlying physiological processes is not only important for getting further insights into the adaptability of photosynthesis, but will help to optimize the biotechnological application of algae as H2 producers. Two of the still most disputed questions regarding H2 generation by C. reinhardtii concern the electron source for H2 evolution and the competition of the hydrogenase with alternative electron sinks. We analyzed the H2 metabolism of S-depleted C. reinhardtii cultures utilizing a special mass spectrometer setup and investigated the influence of photosystem II (PSII)- or ribulosebisphosphate-carboxylase/oxygenase (Rubisco)-deficiency. We show that electrons for H2-production are provided both by PSII activity and by a non-photochemical plastoquinone reduction pathway, which is dependent on previous PSII activity. In a Rubisco-deficient strain, which produces H2 also in the presence of sulfur, H2 generation seems to be the only significant electron sink for PSII activity and rescues this strain at least partially from a light-sensitive phenotype. The latter indicates that the down-regulation of assimilatory pathways in S-deprived C. reinhardtii cells is one of the important prerequisites for a sustained H2 evolution.
This paper references
10.1007/s11120-004-2050-2
Genomics of green algal hydrogen research
A. Melis (2004)
10.1016/0022-2836(73)90198-8
Maturation of the head of bacteriophage T4. I. DNA packaging events.
U. K. Laemmli (1973)
[The photochemical activity of photosystem II in sulfur-deprived Chlamydomonas reinhardtii cells depends on the redox state of the quinone pool during the transition to anaerobiosis].
T. Antal (2004)
10.1007/s004250100660
Biochemical and morphological characterization of sulfur-deprived and H2-producing Chlamydomonas reinhardtii (green alga)
L. Zhang (2001)
tained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii
A Melis (2000)
Interrelation of the mechanisms for oxygen and hydrogen evolution in adapted algae
N. Bishop (1963)
10.1016/c2009-0-02778-0
The Chlamydomonas sourcebook
E. Harris (2009)
10.1104/PP.010498
Hydrogen production. Green algae as a source of energy.
A. Melis (2001)
10.1093/PCP/PCG020
Effects of extracellular pH on the metabolic pathways in sulfur-deprived, H2-producing Chlamydomonas reinhardtii cultures.
S. Kosourov (2003)
10.1016/J.BBABIO.2003.09.008
The dependence of algal H2 production on Photosystem II and O2 consumption activities in sulfur-deprived Chlamydomonas reinhardtii cells.
T. Antal (2003)
10.1046/J.0014-2956.2001.02743.X
Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii.
T. Happe (2002)
10.1074/jbc.274.15.10466
Photosystem I Is Indispensable for Photoautotrophic Growth, CO2 Fixation, and H2 Photoproduction inChlamydomonas reinhardtii *
K. Redding (1999)
10.1104/PP.69.6.1268
H(2) and CO(2) Evolution by Anaerobically Adapted Chlamydomonas reinhardtii F-60.
E. Bamberger (1982)
10.1046/J.0960-7412.2001.01209.X
Small changes in the activity of chloroplastic NADP(+)-dependent ferredoxin oxidoreductase lead to impaired plant growth and restrict photosynthetic activity of transgenic tobacco plants.
Mohammad-Reza Hajirezaei (2002)
10.1128/AEM.71.10.6199-6205.2005
Autotrophic and Mixotrophic Hydrogen Photoproduction in Sulfur-Deprived Chlamydomonas Cells
S. Fouchard (2005)
10.1104/pp.112.3.1005
Interactions between Photosynthesis and Respiration in the Green Alga Chlamydomonas reinhardtii (Characterization of Light-Enhanced Dark Respiration)
X. Xue (1996)
10.1074/jbc.M908732199
Electron Flow between Photosystem II and Oxygen in Chloroplasts of Photosystem I-deficient Algae Is Mediated by a Quinol Oxidase Involved in Chlororespiration*
L. Cournac (2000)
10.1093/emboj/17.4.868
Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes
P. Burrows (1998)
10.1104/PP.123.4.1337
Targeted inactivation of the plastid ndhB gene in tobacco results in an enhanced sensitivity of photosynthesis to moderate stomatal closure.
E. Horvath (2000)
10.1104/PP.117.1.129
The regulation of photosynthetic electron transport during nutrient deprivation in Chlamydomonas reinhardtii.
D. Wykoff (1998)
10.1104/PP.122.1.127
Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii.
A. Melis (2000)
10.1007/BF02787851
Oxygen sensitivity of algal H2-production
M. Ghirardi (1997)
10.1007/s11120-004-7157-y
Role of SulP, a nuclear-encoded chloroplast sulfate permease, in sulfate transport and H2evolution in Chlamydomonas reinhardtii
Hsu-Ching Chen (2004)
10.1085/JGP.26.2.219
FERMENTATIVE AND PHOTOCHEMICAL PRODUCTION OF HYDROGEN IN ALGAE
H. Gaffron (1942)
Fermentative Metabolism of Chlamydomonas reinhardtii
R. P. Gfeller
10.1074/JBC.M008470200
A Novel Type of Iron Hydrogenase in the Green AlgaScenedesmus obliquus Is Linked to the Photosynthetic Electron Transport Chain*
L. Florin (2001)
10.1111/J.1432-1033.1994.TB18923.X
Induction, localization and metal content of hydrogenase in the green alga Chlamydomonas reinhardtii.
T. Happe (1994)
10.1007/978-0-585-35132-2_31
Photosynthetic hydrogen and oxygen production by green algae
E. Greenbaum (1997)
10.1016/0022-2836(73)90199-X
Maturation of the head of bacteriophage T4. II. Head-related, aberrant tau-particles.
U. K. Laemmli (1973)
10.1016/J.BBABIO.2005.04.003
Interruption of the Calvin cycle inhibits the repair of Photosystem II from photodamage.
S. Takahashi (2005)
10.1007/BF00427200
NADH as electron donor for the photosynthetic membrane of Chlamydomonas reinhardii
D. Godde (2004)
10.1105/tpc.104.021972
Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii
M. Posewitz (2004)
10.1104/pp.001636
In Vivo Interactions between Photosynthesis, Mitorespiration, and Chlororespiration in Chlamydomonas reinhardtii
L. Cournac (2002)
10.1111/J.1432-1033.1993.TB17944.X
Isolation, characterization and N-terminal amino acid sequence of hydrogenase from the green alga Chlamydomonas reinhardtii.
T. Happe (1993)
10.1074/jbc.M503840200
Improved Photobiological H2 Production in Engineered Green Algal Cells*
O. Kruse (2005)
10.1016/B978-008044356-0/50010-5
Identification of a Cis-Acting Element Controlling Anaerobic Expression of the Hyda Gene from Chlamydomonas Reinhardtii
M. Stirnberg (2004)
Chlororespiration
G Peltier (2002)
10.1016/J.BBABIO.2005.06.004
Modulation of photosynthetic electron transport in the absence of terminal electron acceptors: characterization of the rbcL deletion mutant of tobacco.
Y. Allahverdiyeva (2005)
10.1104/PP.24.1.1
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.
D. Arnon (1949)
10.1104/PP.75.1.212
Fermentative Metabolism of Chlamydomonas reinhardtii: I. Analysis of Fermentative Products from Starch in Dark and Light.
R. P. Gfeller (1984)
10.1104/PP.77.2.509
Fermentative Metabolism of Chlamydomonas reinhardtii: II. Role of Plastoquinone.
R. P. Gfeller (1985)
10.1097/00010694-196408000-00019
Photosynthetic Mechanisms of Green Plants
B. Kok (1964)
10.1073/PNAS.79.14.4352
Evidence for a respiratory chain in the chloroplast.
P. Bennoun (1982)
10.1007/BF00015058
The role of carbon dioxide in light-activated hydrogen production by Chlamydomonas reinhardtii
Roehl M. Cinco (2004)
10.1016/J.IJHYDENE.2005.04.028
Biochemistry of hydrogen metabolism in Chlamydomonas reinhardtii wild type and a Rubisco-less mutant
Andrea L White (2006)
10.1046/J.1365-313X.2003.01809.X
Transgenic tobacco plants expressing antisense ferredoxin-NADP(H) reductase transcripts display increased susceptibility to photo-oxidative damage.
J. Palatnik (2003)
10.1023/B:PRES.0000030421.31730.cb
Trails of Green Alga Hydrogen Research – from Hans Gaffron to New Frontiers
A. Melis (2004)
10.1042/BST0330039
The exceptional photofermentative hydrogen metabolism of the green alga Chlamydomonas reinhardtii.
A. Hemschemeier (2005)
10.1007/BF00383990
The mechanism of hydrogen photoproduction by several algae
T. S. Stuart (2004)
10.1007/BF00383990
Mechanism of hydrogen photoproduction in several algae II. Contribution of photo-system II
T. S. Stuart (1972)
10.1016/J.BBABIO.2005.05.003
Inhibitor studies on non-photochemical plastoquinone reduction and H(2) photoproduction in Chlamydomonas reinhardtii.
Florence Mus (2005)



This paper is referenced by
10.1016/J.IJHYDENE.2014.04.045
The enhancement mechanism of hydrogen photoproduction in Chlorella protothecoides under nitrogen limitation and sulfur deprivation
L. Zhang (2014)
10.1104/pp.16.01063
Microoxic Niches within the Thylakoid Stroma of Air-Grown Chlamydomonas reinhardtii Protect [FeFe]-Hydrogenase and Support Hydrogen Production under Fully Aerobic Environment1[OPEN]
O. Liran (2016)
10.1186/s13068-018-1030-2
Improved photobio-H2 production regulated by artificial miRNA targeting psbA in green microalga Chlamydomonas reinhardtii
H. Li (2018)
10.1016/J.IJHYDENE.2011.04.229
Functional analysis of hydrogen photoproduction in respiratory-deficient mutants of Chlamydomonas re
Renaud Lecler (2011)
10.1016/J.IJHYDENE.2018.12.049
Reconstruction of a genome-scale metabolic model for Auxenochlorella protothecoides to study hydrogen production under anaerobiosis using multiple optimal solutions
Mahsa Mekanik (2019)
10.3390/plants9091136
Hydrogenases and the Role of Molecular Hydrogen in Plants
G. Russell (2020)
10.1007/s00425-017-2816-3
Bioenergetic reprogramming plasticity under nitrogen depletion by the unicellular green alga Scenedesmus obliquus
Aikaterini Papazi (2017)
10.1016/j.jbiotec.2012.05.010
Artificial microRNA-mediated knockdown of pyruvate formate lyase (PFL1) provides evidence for an active 3-hydroxybutyrate production pathway in the green alga Chlamydomonas reinhardtii.
S. J. Burgess (2012)
10.1016/J.IJHYDENE.2009.09.011
Relationships between H2 photoproduction and different electron transport pathways in sulfur-deprived Chlamydomonas reinhardtii
T. Antal (2009)
10.1016/j.ejcb.2010.06.018
Multiple ferredoxin isoforms in Chlamydomonas reinhardtii - their role under stress conditions and biotechnological implications.
M. Winkler (2010)
10.1016/j.bbabio.2018.01.006
Rational redesign of the ferredoxin-NADP+-oxido-reductase/ferredoxin-interaction for photosynthesis-dependent H2-production.
K. Wiegand (2018)
10.1074/jbc.M109.053496
Characterization of the Key Step for Light-driven Hydrogen Evolution in Green Algae*
Martin Winkler (2009)
10.1007/978-3-319-33778-4
Microbial Resources for Sustainable Energy
B. K. Behera (2016)
10.1016/j.bbabio.2011.02.010
Alternative photosynthetic electron transport pathways during anaerobiosis in the green alga Chlamydomonas reinhardtii.
A. Hemschemeier (2011)
10.1007/s00294-011-0339-1
The chloroplast proteome: a survey from the Chlamydomonas reinhardtii perspective with a focus on distinctive features
Mia Terashima (2011)
10.1007/s11120-010-9582-4
Hydrogen photo-evolution upon S deprivation stepwise: an illustration of microalgal photosynthetic and metabolic flexibility and a step stone for future biotechnological methods of renewable H2 production
Bart Ghysels (2010)
10.1016/J.BCAB.2019.01.011
Transcript analysis of hydrogenase A in an indigenous microalga, Coelastrella sp. M-60
R. Karpagam (2019)
10.1002/9781118659892.CH8
Hydrogen Production by Microalgae
H. Amaro (2013)
10.1016/B978-0-12-800776-1.00025-X
Genetic Optimization of Microalgae for Biohydrogen Production
S. Oncel (2015)
10.1016/J.IJHYDENE.2012.01.068
Sustained hydrogen photoproduction by phosphorus- deprived Chlamydomonas reinhardtii cultures
Khorcheska A. Batyrova (2012)
10.1039/C8EE00054A
A new approach for sustained and efficient H2 photoproduction by Chlamydomonas reinhardtii
Sergey Kosourov (2018)
10.1007/s11120-015-0152-7
Relevance of nutrient media composition for hydrogen production in Chlamydomonas
David González-Ballester (2015)
10.1007/s11120-016-0232-3
Temperature-sensitive PSII: a novel approach for sustained photosynthetic hydrogen production
Vinzenz Bayro-Kaiser (2016)
10.1007/978-81-322-3577-4_9
Biohydrogen Production from Microalgae: An Enzyme Perspective
A. Kose (2017)
10.1073/pnas.1220645110
Increased photosystem II stability promotes H2 production in sulfur-deprived Chlamydomonas reinhardtii
A. Volgusheva (2013)
10.1007/978-94-017-8554-9_13
Photobioreactors Design for Hydrogen Production
J. M. Fernández-Sevilla (2014)
Novel structures of PII signal transduction proteins from oxygenic phototropic organisms
V. Chellamuthu (2014)
10.1016/j.jbiotec.2010.01.023
Improved hydrogen production with expression of hemH and lba genes in chloroplast of Chlamydomonas reinhardtii.
S. Wu (2010)
10.3109/07388551.2012.681625
Potential for green microalgae to produce hydrogen, pharmaceuticals and other high value products in a combined process
K. Skjånes (2013)
Caractérisation et modélisation de la culture de la microalgue Chlamydomonas reinhardtii et mise en place d'un photobioréacteur miniaturisé pour l'étude quantitative de la croissance de micro-organismes photosynthétiques
Nastasia Soanen (2017)
10.1093/jxb/erz380
Characterization of the transient fluorescence wave phenomenon that occurs during H2 production in Chlamydomonas reinhardtii
P. S. Krishna (2019)
10.4155/fseb2013.14.269
Recent developments in light-driven H2 production by the green alga, Chlamydomonas reinhardtii
Khorcheska A. Batyrova (2015)
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