Please confirm you are human (Sign Up for free to never see this)
← Back to Search
Hydrogen Production By Chlamydomonas Reinhardtii: An Elaborate Interplay Of Electron Sources And Sinks
A. Hemschemeier, S. Fouchard, L. Cournac, G. Peltier, T. Happe
Published 2007 · Biology, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
The unicellular green alga Chlamydomonas reinhardtii possesses a [FeFe]-hydrogenase HydA1 (EC 18.104.22.168), which is coupled to the photosynthetic electron transport chain. Large amounts of H2 are produced in a light-dependent reaction for several days when C. reinhardtii cells are deprived of sulfur. Under these conditions, the cells drastically change their physiology from aerobic photosynthetic growth to an anaerobic resting state. The understanding of the underlying physiological processes is not only important for getting further insights into the adaptability of photosynthesis, but will help to optimize the biotechnological application of algae as H2 producers. Two of the still most disputed questions regarding H2 generation by C. reinhardtii concern the electron source for H2 evolution and the competition of the hydrogenase with alternative electron sinks. We analyzed the H2 metabolism of S-depleted C. reinhardtii cultures utilizing a special mass spectrometer setup and investigated the influence of photosystem II (PSII)- or ribulosebisphosphate-carboxylase/oxygenase (Rubisco)-deficiency. We show that electrons for H2-production are provided both by PSII activity and by a non-photochemical plastoquinone reduction pathway, which is dependent on previous PSII activity. In a Rubisco-deficient strain, which produces H2 also in the presence of sulfur, H2 generation seems to be the only significant electron sink for PSII activity and rescues this strain at least partially from a light-sensitive phenotype. The latter indicates that the down-regulation of assimilatory pathways in S-deprived C. reinhardtii cells is one of the important prerequisites for a sustained H2 evolution.
This paper references
Genomics of green algal hydrogen research
A. Melis (2004)
Maturation of the head of bacteriophage T4. I. DNA packaging events.
U. K. Laemmli (1973)
[The photochemical activity of photosystem II in sulfur-deprived Chlamydomonas reinhardtii cells depends on the redox state of the quinone pool during the transition to anaerobiosis].
T. Antal (2004)
Biochemical and morphological characterization of sulfur-deprived and H2-producing Chlamydomonas reinhardtii (green alga)
L. Zhang (2001)
tained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii
A Melis (2000)
Interrelation of the mechanisms for oxygen and hydrogen evolution in adapted algae
N. Bishop (1963)
The Chlamydomonas sourcebook
E. Harris (2009)
Hydrogen production. Green algae as a source of energy.
A. Melis (2001)
Effects of extracellular pH on the metabolic pathways in sulfur-deprived, H2-producing Chlamydomonas reinhardtii cultures.
S. Kosourov (2003)
The dependence of algal H2 production on Photosystem II and O2 consumption activities in sulfur-deprived Chlamydomonas reinhardtii cells.
T. Antal (2003)
Differential regulation of the Fe-hydrogenase during anaerobic adaptation in the green alga Chlamydomonas reinhardtii.
T. Happe (2002)
Photosystem I Is Indispensable for Photoautotrophic Growth, CO2 Fixation, and H2 Photoproduction inChlamydomonas reinhardtii *
K. Redding (1999)
H(2) and CO(2) Evolution by Anaerobically Adapted Chlamydomonas reinhardtii F-60.
E. Bamberger (1982)
Small changes in the activity of chloroplastic NADP(+)-dependent ferredoxin oxidoreductase lead to impaired plant growth and restrict photosynthetic activity of transgenic tobacco plants.
Mohammad-Reza Hajirezaei (2002)
Autotrophic and Mixotrophic Hydrogen Photoproduction in Sulfur-Deprived Chlamydomonas Cells
S. Fouchard (2005)
Interactions between Photosynthesis and Respiration in the Green Alga Chlamydomonas reinhardtii (Characterization of Light-Enhanced Dark Respiration)
X. Xue (1996)
Electron Flow between Photosystem II and Oxygen in Chloroplasts of Photosystem I-deficient Algae Is Mediated by a Quinol Oxidase Involved in Chlororespiration*
L. Cournac (2000)
Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes
P. Burrows (1998)
Targeted inactivation of the plastid ndhB gene in tobacco results in an enhanced sensitivity of photosynthesis to moderate stomatal closure.
E. Horvath (2000)
The regulation of photosynthetic electron transport during nutrient deprivation in Chlamydomonas reinhardtii.
D. Wykoff (1998)
Sustained photobiological hydrogen gas production upon reversible inactivation of oxygen evolution in the green alga Chlamydomonas reinhardtii.
A. Melis (2000)
Oxygen sensitivity of algal H2-production
M. Ghirardi (1997)
Role of SulP, a nuclear-encoded chloroplast sulfate permease, in sulfate transport and H2evolution in Chlamydomonas reinhardtii
Hsu-Ching Chen (2004)
FERMENTATIVE AND PHOTOCHEMICAL PRODUCTION OF HYDROGEN IN ALGAE
H. Gaffron (1942)
Fermentative Metabolism of Chlamydomonas reinhardtii
R. P. Gfeller
A Novel Type of Iron Hydrogenase in the Green AlgaScenedesmus obliquus Is Linked to the Photosynthetic Electron Transport Chain*
L. Florin (2001)
Induction, localization and metal content of hydrogenase in the green alga Chlamydomonas reinhardtii.
T. Happe (1994)
Photosynthetic hydrogen and oxygen production by green algae
E. Greenbaum (1997)
Maturation of the head of bacteriophage T4. II. Head-related, aberrant tau-particles.
U. K. Laemmli (1973)
Interruption of the Calvin cycle inhibits the repair of Photosystem II from photodamage.
S. Takahashi (2005)
NADH as electron donor for the photosynthetic membrane of Chlamydomonas reinhardii
D. Godde (2004)
Hydrogen Photoproduction Is Attenuated by Disruption of an Isoamylase Gene in Chlamydomonas reinhardtii
M. Posewitz (2004)
In Vivo Interactions between Photosynthesis, Mitorespiration, and Chlororespiration in Chlamydomonas reinhardtii
L. Cournac (2002)
Isolation, characterization and N-terminal amino acid sequence of hydrogenase from the green alga Chlamydomonas reinhardtii.
T. Happe (1993)
Improved Photobiological H2 Production in Engineered Green Algal Cells*
O. Kruse (2005)
Identification of a Cis-Acting Element Controlling Anaerobic Expression of the Hyda Gene from Chlamydomonas Reinhardtii
M. Stirnberg (2004)
G Peltier (2002)
Modulation of photosynthetic electron transport in the absence of terminal electron acceptors: characterization of the rbcL deletion mutant of tobacco.
Y. Allahverdiyeva (2005)
COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARIS.
D. Arnon (1949)
Fermentative Metabolism of Chlamydomonas reinhardtii: I. Analysis of Fermentative Products from Starch in Dark and Light.
R. P. Gfeller (1984)
Fermentative Metabolism of Chlamydomonas reinhardtii: II. Role of Plastoquinone.
R. P. Gfeller (1985)
Photosynthetic Mechanisms of Green Plants
B. Kok (1964)
Evidence for a respiratory chain in the chloroplast.
P. Bennoun (1982)
The role of carbon dioxide in light-activated hydrogen production by Chlamydomonas reinhardtii
Roehl M. Cinco (2004)
Biochemistry of hydrogen metabolism in Chlamydomonas reinhardtii wild type and a Rubisco-less mutant
Andrea L White (2006)
Transgenic tobacco plants expressing antisense ferredoxin-NADP(H) reductase transcripts display increased susceptibility to photo-oxidative damage.
J. Palatnik (2003)
Trails of Green Alga Hydrogen Research – from Hans Gaffron to New Frontiers
A. Melis (2004)
The exceptional photofermentative hydrogen metabolism of the green alga Chlamydomonas reinhardtii.
A. Hemschemeier (2005)
The mechanism of hydrogen photoproduction by several algae
T. S. Stuart (2004)
Mechanism of hydrogen photoproduction in several algae II. Contribution of photo-system II
T. S. Stuart (1972)
Inhibitor studies on non-photochemical plastoquinone reduction and H(2) photoproduction in Chlamydomonas reinhardtii.
Florence Mus (2005)
This paper is referenced by
The enhancement mechanism of hydrogen photoproduction in Chlorella protothecoides under nitrogen limitation and sulfur deprivation
L. Zhang (2014)
Microoxic Niches within the Thylakoid Stroma of Air-Grown Chlamydomonas reinhardtii Protect [FeFe]-Hydrogenase and Support Hydrogen Production under Fully Aerobic Environment1[OPEN]
O. Liran (2016)
Improved photobio-H2 production regulated by artificial miRNA targeting psbA in green microalga Chlamydomonas reinhardtii
H. Li (2018)
Functional analysis of hydrogen photoproduction in respiratory-deficient mutants of Chlamydomonas re
Renaud Lecler (2011)
Reconstruction of a genome-scale metabolic model for Auxenochlorella protothecoides to study hydrogen production under anaerobiosis using multiple optimal solutions
Mahsa Mekanik (2019)
Hydrogenases and the Role of Molecular Hydrogen in Plants
G. Russell (2020)
Bioenergetic reprogramming plasticity under nitrogen depletion by the unicellular green alga Scenedesmus obliquus
Aikaterini Papazi (2017)
Artificial microRNA-mediated knockdown of pyruvate formate lyase (PFL1) provides evidence for an active 3-hydroxybutyrate production pathway in the green alga Chlamydomonas reinhardtii.
S. J. Burgess (2012)
Relationships between H2 photoproduction and different electron transport pathways in sulfur-deprived Chlamydomonas reinhardtii
T. Antal (2009)
Multiple ferredoxin isoforms in Chlamydomonas reinhardtii - their role under stress conditions and biotechnological implications.
M. Winkler (2010)
Rational redesign of the ferredoxin-NADP+-oxido-reductase/ferredoxin-interaction for photosynthesis-dependent H2-production.
K. Wiegand (2018)
Characterization of the Key Step for Light-driven Hydrogen Evolution in Green Algae*
Martin Winkler (2009)
Microbial Resources for Sustainable Energy
B. K. Behera (2016)
Alternative photosynthetic electron transport pathways during anaerobiosis in the green alga Chlamydomonas reinhardtii.
A. Hemschemeier (2011)
The chloroplast proteome: a survey from the Chlamydomonas reinhardtii perspective with a focus on distinctive features
Mia Terashima (2011)
Hydrogen photo-evolution upon S deprivation stepwise: an illustration of microalgal photosynthetic and metabolic flexibility and a step stone for future biotechnological methods of renewable H2 production
Bart Ghysels (2010)
Transcript analysis of hydrogenase A in an indigenous microalga, Coelastrella sp. M-60
R. Karpagam (2019)
Hydrogen Production by Microalgae
H. Amaro (2013)
Genetic Optimization of Microalgae for Biohydrogen Production
S. Oncel (2015)
Sustained hydrogen photoproduction by phosphorus- deprived Chlamydomonas reinhardtii cultures
Khorcheska A. Batyrova (2012)
A new approach for sustained and efficient H2 photoproduction by Chlamydomonas reinhardtii
Sergey Kosourov (2018)
Relevance of nutrient media composition for hydrogen production in Chlamydomonas
David González-Ballester (2015)
Temperature-sensitive PSII: a novel approach for sustained photosynthetic hydrogen production
Vinzenz Bayro-Kaiser (2016)
Biohydrogen Production from Microalgae: An Enzyme Perspective
A. Kose (2017)
Increased photosystem II stability promotes H2 production in sulfur-deprived Chlamydomonas reinhardtii
A. Volgusheva (2013)
Photobioreactors Design for Hydrogen Production
J. M. Fernández-Sevilla (2014)
Novel structures of PII signal transduction proteins from oxygenic phototropic organisms
V. Chellamuthu (2014)
Improved hydrogen production with expression of hemH and lba genes in chloroplast of Chlamydomonas reinhardtii.
S. Wu (2010)
Potential for green microalgae to produce hydrogen, pharmaceuticals and other high value products in a combined process
K. Skjånes (2013)
Caractérisation et modélisation de la culture de la microalgue Chlamydomonas reinhardtii et mise en place d'un photobioréacteur miniaturisé pour l'étude quantitative de la croissance de micro-organismes photosynthétiques
Nastasia Soanen (2017)
Characterization of the transient fluorescence wave phenomenon that occurs during H2 production in Chlamydomonas reinhardtii
P. S. Krishna (2019)
Recent developments in light-driven H2 production by the green alga, Chlamydomonas reinhardtii
Khorcheska A. Batyrova (2015)See more