← Back to Search
Short-term Salinity Stress In Tobacco Plants Leads To The Onset Of Animal-like PCD Hallmarks In Planta In Contrast To Long-term Stress
Efthimios Andronis, K. A. Roubelakis-Angelakis
Published 2009 · Medicine, Biology
Download PDFAnalyze on Scholarcy
Recent results have identified mitochondria as centers of stress-induced generation of reactive oxygen species in plants. Depolarization of plant mitochondrial membrane during stress results the release of programmed cell death (PCD)-inducing factors in the cytosol in a fashion similar to the onset of animal-like PCD. Herein, we report significant similarities of animal-like PCD and salinity stress-induced plant PCD. Short-term salinity stress (3 h) led to depolarization of the mitochondrial membrane, release of cytochrome c (CYT-c), which was visualized using a contemporary molecular technique, activation of caspase-3 type proteases and the onset of PCD in wild type tobacco plants, Nicotiana tabacum cv. Petit Havana. However, PCD was not manifested during long-term salinity stress (24 h). Interestingly long-term salinity stress led to necrotic-like features, which were accompanied by collapse of respiration, reduction of key components of the respiratory chain, such as CYT-c and alternative oxidase, ATP depletion and high proteolytic activity. The results suggest that salinity stress of tobacco plants in planta leads to the onset of animal-like PCD only during the early stages post-stress, while long-term stress leads to necrotic-like features.
This paper references
State transitions, cyclic and linear electron transport and photophosphorylation in Chlamydomonas reinhardtii.
G. Finazzi (1999)
Production of Reactive Oxygen Species, Alteration of Cytosolic Ascorbate Peroxidase, and Impairment of Mitochondrial Metabolism Are Early Events in Heat Shock-Induced Programmed Cell Death in Tobacco Bright-Yellow 2 Cells1
R. A. Vacca (2004)
The presence and subcellular localization of caspase 3‐like proteinases in plant cells
H. Korthout (2000)
Activation of Chlororespiration Increases Chlorophyll Fluorescence Yield in Chlorella Adapted to Darkness at High Temperature
Yu. K. Chemeris (2004)
Cytochrome c in the apoptotic and antioxidant cascades
V. Skulachev (1998)
Mitochondrial Reactive Oxygen Species. Contribution to Oxidative Stress and Interorganellar Signaling
D. Rhoads (2006)
Oxidative Stress Increased Respiration and Generation of Reactive Oxygen Species, Resulting in ATP Depletion, Opening of Mitochondrial Permeability Transition, and Programmed Cell Death1
B. Tiwari (2002)
In vitro characterization of a plastid terminal oxidase (PTOX).
Eve-Marie Josse (2003)
Many ways to exit? Cell death categories in plants.
W. G. van Doorn (2005)
The generation of active oxygen species differs in tobacco and grapevine mesophyll protoplasts.
A. Papadakis (1999)
H Thordal-Christensen (1997)
High-quality RNA, cDNA, and derived EST libraries from grapevine (Vitis vinifera L.)
A. Iandolino (2007)
Translocation of cytochrome c from the mitochondria to the cytosol occurs during heat‐induced programmed cell death in cucumber plants
J. Balk (1999)
Mechanisms and functions of cell death.
R. Ellis (1991)
Mitochondria and cell death: a pore way to die?
A. Halestrap (2000)
The evolution of a mechanism of cell suicide.
N. Blackstone (1999)
Mitochondrial redox biology and homeostasis in plants.
G. Noctor (2007)
Polyamines inhibit NADPH oxidase-mediated superoxide generation and putrescine prevents programmed cell death induced by polyamine oxidase-generated hydrogen peroxide
A. Papadakis (2004)
Chlororespiration is involved in the adaptation of Brassica plants to heat and high light intensity.
M. Díaz (2007)
Transgenic Plant Cells Lacking Mitochondrial Alternative Oxidase Have Increased Susceptibility to Mitochondria-Dependent and -Independent Pathways of Programmed Cell Death1
C. A. Robson (2002)
Does the plant mitochondrion integrate cellular stress and regulate programmed cell death?
A. Jones (2000)
Chloroplastic NAD(P)H Dehydrogenase in Tobacco Leaves Functions in Alleviation of Oxidative Damage Caused by Temperature Stress1[OA]
P. Wang (2006)
Substrate Specificities of Caspase Family Proteases*
R. Talanian (1997)
Initiation of Runaway Cell Death in an Arabidopsis Mutant by Extracellular Superoxide
T. Jabs (1996)
A cytochrome c mutant with high electron transfer and antioxidant activities but devoid of apoptogenic effect.
Z. K. Abdullaev (2002)
Mitochondrial alternative oxidase acts to dampen the generation of active oxygen species during a period of rapid respiration induced to support a high rate of nutrient uptake.
J. Y. Yip (2001)
Cytochrome c Is Released in a Reactive Oxygen Species-Dependent Manner and Is Degraded via Caspase-Like Proteases in Tobacco Bright-Yellow 2 Cells en Route to Heat Shock-Induced Cell Death1
R. A. Vacca (2006)
Effect of temperature
R. Brown (1996)
Effect of tordon 2,4‐D 64/240 triethanolamine BR on the energy metabolism of rat liver mitochondria
L. F. Pereira (1994)
Activation of alternative oxidase and uncoupling protein lowers hydrogen peroxide formation in amoeba Acanthamoeba castellanii mitochondria
M. Czarna (2005)
Plant salt tolerance.
J. K. Zhu (2001)
Die and let live - programmed cell death in plants.
E. Lam (1999)
Spermidine Exodus and Oxidation in the Apoplast Induced by Abiotic Stress Is Responsible for H2O2 Signatures That Direct Tolerance Responses in Tobacco[W]
P. Moschou (2008)
Mitochondria and apoptosis.
D. Green (1998)
Hydrogen peroxide generation by higher plant mitochondria oxidizing complex I or complex II substrates
E. Braidot (1999)
Cytochrome c is released from coupled mitochondria of yeast en route to acetic acid‐induced programmed cell death and can work as an electron donor and a ROS scavenger
S. Giannattasio (2008)
Are mitochondria a permanent source of reactive oxygen species?
K. Staniek (2000)
Evaluation of fish decomposition by liquid chromatographic assay of ATP degradation products
M. Valle (1998)
Production and Scavenging of Reactive Oxygen Species in Chloroplasts and Their Functions1
K. Asada (2006)
Subcellular localization of H2O2 in plants. H2O2 accumulation in papillae and hypersensitive response during the barley—powdery mildew interaction
H. Thordal-Christensen (1997)
Monoclonal antibodies to the alternative oxidase of higher plant mitochondria.
T. Elthon (1989)
Rapid screening for improved solubility of small human proteins produced as fusion proteins in Escherichia coli
Martin Hammarström (2002)
Mitochondrial damage induced by conditions of oxidative stress.
A. Kowaltowski (1999)
Cell Death in Development
D. Vaux (1999)
The antioxidant functions of cytochrome c
S. S. Korshunov (1999)
Comparative physiology of salt and water stress.
R. Munns (2002)
THE OXIDATIVE BURST IN PLANT DISEASE RESISTANCE.
C. Lamb (1997)
A plastid terminal oxidase associated with carotenoid desaturation during chromoplast differentiation.
E. Josse (2000)
Transgenic tobacco plants overexpressing polyamine oxidase are not able to cope with oxidative burst generated by abiotic factors.
P. Moschou (2008)
Programmed cell death in plants: new insights into redox regulation and the role of hydrogen peroxide.
Ilya Gadjev (2008)
Dinitro-o-cresol induces apoptosis-like cell death but not alternative oxidase expression in soybean cells.
M. M. Aranha (2007)
Caspases and programmed cell death in the hypersensitive response of plants to pathogens
Olga María del Pozo (1998)
Monitoring the mitochondrial transmembrane potential with the JC-1 fluorochrome in programmed cell death during mesophyll leaf senescence
E. Simeonova (2004)
The antioxidant functions of cytochrome
SS Korshunov (1999)
Abiotic Stress Generates ROS That Signal Expression of Anionic Glutamate Dehydrogenases to Form Glutamate for Proline Synthesis in Tobacco and Grapevine[W]
D. S. Skopelitis (2006)
The programme of cell death in plants and animals - a comparison.
K. Krishnamurthy (2000)
ROS, mitochondria and the regulation of autophagy.
Ruth Scherz-Shouval (2007)
Programmed cell death during pollination-induced petal senescence in petunia.
Y. Xu (2000)
Effect of temperature on rates of alternative and cytochrome pathway respiration and their relationship with the redox poise of the quinone pool.
O. Atkin (2002)
Cytochrome c release and caspase activation during menadione‐induced apoptosis in plants
Y. Sun (1999)
This paper is referenced by
Polyamines as redox homeostasis regulators during salt stress in plants
J. Saha (2015)
Spatial and temporal nature of reactive oxygen species production and programmed cell death in elm (Ulmus pumila L.) seeds during controlled deterioration.
Die Hu (2012)
Caractérisation de suppresseurs de la mort cellulaire programmée chez Arabidopsis thaliana
Quentin Bruggeman (2014)
Comparative study of salt stress-induced physiological and molecular responses in tomato (Solanum lycopersicum L.)
Kovács Judit (2017)
Ethylene Antagonizes Salt-Induced Growth Retardation and Cell Death Process via Transcriptional Controlling of Ethylene-, BAG- and Senescence-Associated Genes in Arabidopsis
Ya-jie Pan (2016)
High Yields of Hydrogen Production Induced by Meta-Substituted Dichlorophenols Biodegradation from the Green Alga Scenedesmus obliquus
Aikaterini Papazi (2012)
What Do the Plant Mitochondrial Antioxidant and Redox Systems Have to Say Under Salinity, Drought, and Extreme Temperature?
F. Sevilla (2015)
Spatial and temporal progress of programmed cell death in the developing starchy endosperm of rice
Hidekazu Kobayashi (2013)
Alternative Oxidase: A Mitochondrial Respiratory Pathway to Maintain Metabolic and Signaling Homeostasis during Abiotic and Biotic Stress in Plants
G. Vanlerberghe (2013)
Nitric Oxide Action in Abiotic Stress Responses in Plants
M. N. Khan (2015)
The release of cytochrome c and the regulation of the programmed cell death progress in the endosperm of winter wheat (Triticum aestivum L.) under waterlogging
Yuan-Hong Qi (2018)
Polyamine Catabolism in Plants: A Universal Process With Diverse Functions
W. Wang (2019)
Targeting the Redox Regulatory Mechanisms for Abiotic Stress Tolerance in Crops
Punam Kundu (2018)
Evidence for extensive heterotrophic metabolism, antioxidant action, and associated regulatory events during winter hardening in Sitka spruce
Eva Collakova (2012)
Caspase-like enzymatic activity and the ascorbate-glutathione cycle participate in salt stress tolerance of maize conferred by exogenously applied nitric oxide
M. Keyster (2012)
Salt responsive physiological, photosynthetic and biochemical attributes at early seedling stage for screening soybean genotypes.
D. B. Shelke (2017)
Capacity to control oxidative stress-induced caspase-like activity determines the level of tolerance to salt stress in two contrasting maize genotypes
Marshall Keyster (2012)
Proteasome Activity Profiling Uncovers Alteration of Catalytic β2 and β5 Subunits of the Stress-Induced Proteasome during Salinity Stress in Tomato Roots
J. Kovács (2017)
Expression and signal regulation of the alternative oxidase genes under abiotic stresses.
H. Feng (2013)
Green algal molecular responses to temperature stress
B. Barati (2019)
Ultrastructural Changes of Organelles in Root Cap Cells of Tobacco Under Salinity
E. Baranova (2019)
Regulation of cytochrome and alternative pathways under light and osmotic stress
Padmanabh Dwivedi (2015)
Programmed cell death is induced by hydrogen peroxide but not by excessive ionic stress of sodium chloride in the unicellular green alga Chlamydomonas reinhardtii
Sirisha L. Vavilala (2015)
Induction of Programmed Cell Death through mitochondria-dependent pathways in Tamba black soybean (Glycine max)
J-h Li (2015)
Redox regulation in plant programmed cell death.
M. D. de Pinto (2012)
Morpho-Biological and Cytological Characterization of Tomato Roots (Solanum lycopersicum L., cv. Rekordsmen) Regenerated under NaCl Salinity in vitro
L. R. Bogoutdinova (2020)
ROS-Induced Signaling and Gene Expression in Crops Under Salinity Stress
Vinay Kumar (2017)
Polyamines and programmed cell death
Uppsala BioCenter (2013)
Peroxisomal polyamine oxidase and NADPH-oxidase cross-talk for ROS homeostasis which affects respiration rate in Arabidopsis thaliana
Efthimios Andronis (2014)
The role of FMT and FIS1A in mitochondrial morphology and salt stress in Arabidopsis thaliana
Alexandra Ralevski (2016)
Polyamines and programmed cell death.
P. Moschou (2014)
KCl induces a caspase-independent programmed cell death in the unicellular green chlorophyte Chlamydomonas reinhardtii (Chlorophyceae)
Sirisha L. Vavilala (2016)See more