Online citations, reference lists, and bibliographies.
Please confirm you are human
(Sign Up for free to never see this)
← Back to Search

Pax7 Distribution In Human Skeletal Muscle Biopsies And Myogenic Tissue Cultures

J. Reimann, K. Brimah, R. Schroeder, A. Wernig, J. Beauchamp, T. Partridge
Published 2003 · Biology, Medicine

Save to my Library
Download PDF
Analyze on Scholarcy
Demonstration of the importance of the paired box transcription factor Pax7 for the murine myosatellite cell population, with persistent expression in mature skeletal muscle, prompted us to investigate the distribution of Pax7 protein in biopsy samples of normal and pathological human skeletal limb muscle. Immunostaining for M-cadherin, an adhesion molecule present at the interface between myofibre and satellite cell, and the characteristic position adjacent to the muscle fibre and beneath the fibre’s basement membrane were used to identify satellite cells. Anti-Pax7 reactivity was found in the majority of satellite cells but a small population was Pax7 negative. Neither could we identify Pax7-positive nuclei in freshly regenerating myotubes or in presumed myoblasts in these biopsies. Similarly, in myogenic cell cultures derived from the explantation of human foetal muscle Pax7 expression was low or undetectable at the proliferative myoblast stage but it became prominent in an increasing proportion of mononucleate cells after the induction of differentiation. This expression was, however, restricted to mononucleate cells; it did not persist into the differentiation stage of newly formed multinucleate myotubes. Despite this, in the biopsy samples, we occasionally found Pax7-positive nuclei in muscle fibres that seemed to be undergoing degenerative changes. Most of these were found to be the nuclei of cells engaged in focal regenerative processes, but Pax7 re-expression by myonuclei “in distress” cannot be ruled out entirely.
This paper references
Pax genes and their roles in cell differentiation and development.
A. Mansouri (1996)
Expression of Cd34 and Myf5 Defines the Majority of Quiescent Adult Skeletal Muscle Satellite Cells
J. Beauchamp (2000)
Pax3 modulates expression of the c-Met receptor during limb muscle development.
J. Epstein (1996)
Differential myogenicity of satellite cells isolated from extensor digitorum longus (EDL) and soleus rat muscles revealed in vitro
C. Lagord (1998)
Two Critical Periods of Sonic Hedgehog Signaling Required for the Specification of Motor Neuron Identity
J. Ericson (1996)
Pax7 is expressed in the capsules surrounding adult mouse neuromuscular spindles.
J. Rodger (1999)
Satellite cell proliferation and the expression of myogenin and desmin in regenerating skeletal muscle: evidence for two different populations of satellite cells.
Juha Rantanen (1995)
Evidence for multiple satellite cell populations and a non-myogenic cell type that is regulated differently in regenerating and growing skeletal muscle.
G. Molnár (1996)
Kinetics of myoblast proliferation show that resident satellite cells are competent to fully regenerate skeletal muscle fibers.
P. Zammit (2002)
Expression of M-cadherin, a member of the cadherin multigene family, correlates with differentiation of skeletal muscle cells.
M. Donalies (1991)
A quantitative study of myonuclear and satellite cell nuclear size in Duchenne's muscular dystrophy, polymyositis and normal human skeletal muscle
Simon C Watkins (1988)
Biological Progression from Adult Bone Marrow to Mononucleate Muscle Stem Cell to Multinucleate Muscle Fiber in Response to Injury
M. Labarge (2002)
Dynamics of Myoblast Transplantation Reveal a Discrete Minority of Precursors with Stem Cell–like Properties as the Myogenic Source
J. Beauchamp (1999)
Pax7 Is Required for the Specification of Myogenic Satellite Cells
P. Seale (2000)
Binding and activation of the promoter for the neural cell adhesion molecule by Pax-8.
B. Holst (1994)
Expression pattern of M‐cadherin in normal, denervated, and regenerating mouse muscles
A. Irintchev (1994)
The regulation of Notch signaling controls satellite cell activation and cell fate determination in postnatal myogenesis.
I. Conboy (2002)
The formation of skeletal muscle: from somite to limb
M. Buckingham (2003)
Transplanted primary neonatal myoblasts can give rise to functional satellite cells as identified using the Myf5nlacZl+ mouse
L. Heslop (2001)
Mcadherin is a reliable molecular marker of satellite cells in mouse skeletal muscle
JD Rosenblatt (1999)
Fos, Jun, Myo D1 and myogenin proteins are increased in skeletal muscle fibre nuclei after denervation
J Weis (1994)
Jun, Fos, MyoD1, and Myogenin proteins are increased in skeletal muscle fiber nuclei after denervation
J. Weis (2004)

This paper is referenced by
Myogenic skeletal muscle satellite cells communicate by tunnelling nanotubes
P. Tavi (2010)
Hierarchization of Myogenic and Adipogenic Progenitors Within Human Skeletal Muscle
Didier F. Pisani (2010)
Noncoding RNAs as epigenetic mediators of skeletal muscle regeneration
G. Sohi (2015)
Pax7 Shows Higher Satellite Cell Frequencies and Concentrations within Intrafusal Fibers of Muscle Spindles
L. J. Kirkpatrick (2008)
Epigenetic regulation of satellite cell activation during muscle regeneration
F. J. Dilworth (2011)
Characterization of distinct mesenchymal-like cell populations from human skeletal muscle in situ and in vitro.
S. Lecourt (2010)
Isolation of human adult stem cells from muscle biopsy for future treatment of urinary incontinence.
F. Sharifiaghdas (2011)
Are Human and Mouse Satellite Cells Really the Same?
L. Boldrin (2010)
Co‐expression of IGF‐1 family members with myogenic regulatory factors following acute damaging muscle‐lengthening contractions in humans
Bryon R. McKay (2008)
Cellular mechanisms and local progenitor activation to regulate skeletal muscle mass
M. Cassano (2010)
Cell-Surface Protein Profiling Identifies Distinctive Markers of Progenitor Cells in Human Skeletal Muscle
A. Uezumi (2016)
Childhood Rhabdomyosarcoma
C. Wang (2012)
Dorjan Marušič (2012)
The expression patterns of Pax7 in satellite cells during overload‐induced rat adult skeletal muscle hypertrophy
M. Ishido (2009)
Satellite cells depletion in exercising human skeletal muscle is restored by ginseng component Rg1 supplementation
Jinfu Wu (2019)
Papel de la células satélite en la hipertrofia y regeneración muscular en respuesta al ejercicio
A. G. Grau (2007)
Genetic and pharmacological regulation of the endocannabinoid CB1 receptor in Duchenne muscular dystrophy
F. Iannotti (2018)
Overview of Cell Types Capable of Contributing to Skeletal Muscle Repair and Regeneration
Johanna Pruller (2019)
Histopathological features and satellite cell population characteristics in human inferior oblique muscle biopsies: clinicopathological correlation.
A. Baytaroğlu (2020)
Aldehyde dehydrogenase activity promotes survival of human muscle precursor cells
Elise Jean (2011)
Skeletal muscle cell transplantation: models and methods
A. L. Mueller (2019)
New multiple labelling method for improved satellite cell identification in human muscle: application to a cohort of power-lifters and sedentary men
M. Lindström (2009)
Satellite cell number and cell cycle kinetics in response to acute myotrauma in humans: immunohistochemistry versus flow cytometry
Bryon R. McKay (2010)
Marking the tempo for myogenesis: Pax7 and the regulation of muscle stem cell fate decisions
H. Olguín (2012)
Laminin mimetic peptide nanofibers regenerate acute muscle defect.
Cagla Eren Cimenci (2017)
Pax-7 up-regulation inhibits myogenesis and cell cycle progression in satellite cells: a potential mechanism for self-renewal.
H. Olguín (2004)
Cancer Stem Cells: Lessons From Aml
D. Briggs (2016)
Functional properties of muscle-derived cells related to morphological characteristics
G. Jouvion (2006)
Skeletal Muscle Tissue Engineering
G. Christ (2015)
Human and mouse skeletal muscle stem and progenitor cells in health and disease.
Bartosz Mierzejewski (2020)
Satellite cell activation and number following acute and chronic exercise: A mini review
M. Lewis (2012)
The influence of Notch over-stimulation on muscle stem cell quiescence versus proliferation, and on muscle regeneration
Can Ding (2015)
See more
Semantic Scholar Logo Some data provided by SemanticScholar