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Changes In Vascular Responses Of The Basilar Artery To Acetylcholine And Endothelin-1 In An Experimental Rabbit Vasospasm Model

C. Kim, S. H. Paek, B. Seo, J. H. Kim, D. H. Han
Published 2003 · Medicine

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Summary¶Background. This study aimed to clarify the characteristics of the basilar arteries of rabbits two and seven days after subarachnoid haemorrhage (SAH) and to investigate the influence of hypoxia on vascular responses to acetylcholine and endothelin-1.Methods. Thirty four New Zealand white rabbits were divided into 3 groups: 1) control (n=6), 2) 2 days post SAH (n=15), and 3) 7 days post SAH (n=13). Rabbits in the groups 2 and 3 underwent vertebral angiography before sacrifice. Harvested vascular rings were suspended in a small vessel myograph system for isometric tension recording in response to vasodilatory and vasoconstrictory stimulation in oxygenated (95% O2/5% CO2) and hypoxic (95% N2/5% CO2) conditions. To investigate the response to a vasodilator, acetylcholine (ACh) (10−7 to 3×10−4 M) was applied to a basilar artery pre-contracted with histamine (10−6∼10−5 M). To investigate the response to a vasoconstrictor, the effect of endothelin-1 (ET-1) (10−11 to 3×10−8 M) was observed in quiescent rings.Findings. Seven of 15 rabbits that underwent angiography two days after operation showed vasospasm to 64.3±11.2% of the original diameter; likewise seven of the 13 rabbits studied seven days after operation showed vasospasm to 64.9±10.9% of the original diameter. Hypoxic conditions significantly reduced the relaxation response to ACh but did not influence basilar artery contraction to ET-1. Responses to ACh and ET-1 did not differ significantly in vessels from animals with or without vasospasm at 2 days. In contrast, at 7 days vascular responses to ACh and ET-1 were significantly less in vessels from animals with vasospasm than those without (p=0.029, 0.032), and also less than in vessels from animals with vasospasm at day 2 (p=0.002, 0.004). There was not a significant difference in the vascular responses of basilar arteries from animals without vasospasm.Interpretation. The markedly lower vascular responses of basilar arteries of rabbits to ACh and ET-1 found seven days after SAH may relate to delayed vasospasm in patients with SAH.
This paper references
Changes in endothelial nitric oxide synthase mRNA during vasospasm after subarachnoid haemorrhage
A Hino (1996)
Impairment of the modulatory role of nitric oxide on the endothelin-1-elicited contraction of cerebral arteries: a pathogenetic factor in cerebral vasospasm after subarachnoid hemorrhage?
J. A. Alabadí (1997)
Hemoglobin penetration in the wall of the rabbit basilar artery after subarachnoid hemorrhage and intracisternal hemoglobin injection
P. Foley (2005)
Nitric Oxide and the Cerebral Circulation
F. Faraci (1994)
Breakdown products of erythrocytes as a cause of cerebral vasospasm. In Wilkins RH (ed) Cerebral arterial spasm
H Handa (1980)
Effect of subarachnoid hemorrhage on endothelium-dependent vasodilation.
T. Nakagomi (1987)
Selective hemoglobin inhibition of endothelium-dependent vasodilation of rabbit basilar artery.
S. Fujiwara (1986)
Relation of cerebral vasospasm to subarachnoid hemorrhage visualized by computerized tomographic scanning.
C. Fisher (1980)
Modulatory role of endothelial and nonendothelial nitric oxide in 5-hydroxytryptamine-induced contraction in cerebral arteries after subarachnoid hemorrhage.
F. J. Miranda (1996)
Endothelium-dependent relaxation of human pulmonary arteries.
B. Greenberg (1987)
The role of hemoglobin in arterial narrowing after subarachnoid hemorrhage.
M. Mayberg (1990)
Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor
R. M. Palmer (1987)
Subarachnoid hemorrhage inhibition of endothelium-derived relaxing factor in rabbit basilar artery.
K. Hongo (1988)
Endothelial nitric oxide synthase: molecular cloning and characterization of a distinct constitutive enzyme isoform.
S. Lamas (1992)
Increased vasoconstrictor activity of proximal coronary arteries with endothelial damage in intact dogs.
J. Brum (1984)
Cerebral blood flow measurements after spontaneous subarachnoid haemorrhage.
G. Ferguson (1972)
Cellular mechanism of thrombin on endothelin-1 biosynthesis and release in bovine endothelial cell.
T. Emori (1992)
The Prognostic Value of Noninvasive CBF Measurement in Subarachnoid Hemorrhage
G. Géraud (1984)
Cerebrospinal fluid may nourish cerebral vessels through pathways in the adventitia that may be analogous to systemic vasa vasorum.
N. Zervas (1982)
Comparison of spontaneously released endothelium-derived relaxing factor in cerebral and extracerebral arteries in rabbits.
K. Hongo (1993)
Breakdown products of erythrocytes as a cause of cerebral vasospasm
H Handa (1980)
Endothelium-dependent relaxation in experimental atherosclerosis in the rabbit.
L. Jayakody (1987)
Evidence of endothelial dysfunction in angiographically normal coronary arteries of patients with coronary artery disease.
S. Werns (1989)
Combined effect of L-arginine and superoxide dismutase on the spastic basilar artery after subarachnoid hemorrhage in dogs.
Y. Kajita (1994)
Differences in Basal Endothelium‐Derived Relaxing Factor Activity in Different Artery Types
P. Collins (1986)
Endothelium-derived relaxing factor.
J. Angus (1989)
Changes in vasoactive properties of blood products with time and attempted identification of the spasmogens.
F. Okwuasaba (1981)
Effect of hypoxia on endothelium-dependent relaxation of canine and rabbit basilar arteries
T. Nakagomi (2005)
Changes in endothelial nitric oxide synthase mRNA during vasospasm after subarachnoid hemorrhage in monkeys.
A. Hino (1996)
Reduced production of cGMP underlies the loss of endothelium-dependent relaxations in the canine basilar artery after subarachnoid hemorrhage.
P. Kim (1992)
Preliminary characterization of vasocontractile activities in erythrocytes.
S. Cheung (1980)
Vasospasm and regional cerebral blood flow (rCBF) in patients with ruptured intracranial aneurysm: serial rCBF studies with the xenon-133 inhalation method.
I. Yamakami (1983)
Measurement of regional cerebral blood flow in aneurysm surgery.
N. Martin (1985)
Regional cerebral blood flow in patients with aneurysms: estimation by Xenon 133 inhalation.
B. Weir (1978)
Angiographic evidence of basilar artery constriction in the rabbit: a new model of vasospasm.
K. Baker (1987)
Biological Actions and Properties of Endothelium-Derived Nitric Oxide Formed and Released From Artery and Vein
L. Ignarro (1989)
Comparative pharmacology of EDRF and nitric oxide on vascular strips.
P. J. Hutchinson (1987)
Longitudinal time course of reversible and irreversible components of chronic cerebrovasospasm of the rabbit basilar artery.
P. Vorkapic (1991)

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