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Synergistic And Sequential Effects Of BMP-2, BFGF And VEGF On Osteogenic Differentiation Of Rat Osteoblasts
P. Li, Y. Bai, Guangfu Yin, Ximing Pu, Z. Huang, Xiaoming Liao, X. Chen, Y. Yao
Published 2013 · Chemistry, Medicine
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In the present study, the effects of bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) on regulation of rat osteoblast (ROB) maturation in vitro were investigated. It was found that the proliferation, differentiation and mineralization of ROBs were all dose-dependently increased at particular times in the case of treatment with only one growth factor. To investigate the effects of combined treatment, ROBs were treated with either a single application of a relatively high dose of each growth factor, or binary/triple combined applications of relatively low doses of the growth factors. Osteogenic differentiation was significantly promoted in the triple combination treatment of BMP-2, VEGF and bFGF compared with the single or binary combination treatments. The optimal timing of the triple combination to enhance osteogenesis was also tested. When bFGF and VEGF were added in the early stage, and BMP-2 and VEGF were added in the late stage, osteogenic differentiation of ROBs could be enhanced more effectively. These results could be used to construct bone tissue engineering scaffolds that release growth factors sequentially.
This paper references
VEGF Improves, Whereas sFlt1 Inhibits, BMP2‐Induced Bone Formation and Bone Healing Through Modulation of Angiogenesis
H. Peng (2005)
differentiation stagedependent mitogenic effect, inverse regulation of BMP-2 and noggin, and enhancement of osteogenic potential
A Fakhry (2014)
BMP receptor signaling: transcriptional targets, regulation of signals, and signaling cross-talk.
K. Miyazono (2005)
Effects of FGF-2/-9 in calvarial bone cell cultures: differentiation stage-dependent mitogenic effect, inverse regulation of BMP-2 and noggin, and enhancement of osteogenic potential.
Ali Fakhry (2005)
BMP-2 and Insulin-like Growth Factor-I Mediate Osterix (Osx) Expression in Human Mesenchymal Stem Cells via the MAPK and Protein Kinase D Signaling Pathways*
Ayse B. Celil (2005)
Effects of VEGF and bFGF on proliferation and production of steroids and nitric oxide in porcine granulosa cells.
F. Grasselli (2002)
Expression of Vascular Endothelial Growth Factors and Their Receptors during Osteoblast Differentiation.
M. Deckers (2000)
Optimized bone regeneration based on sustained release from three‐dimensional fibrous PLGA/HAp composite scaffolds loaded with BMP‐2
Y. Fu (2008)
Enhancement of osteoblastic differentiation of mesenchymal stromal cells cultured by selective combination of bone morphogenetic protein-2 (BMP-2) and fibroblast growth factor-2 (FGF-2)
前川 尚宜 (2008)
Effects of BMP-2 and vitamin D3 on the osteogenic differentiation of adipose stem cells.
I. Song (2011)
Biology of BMP signalling and cancer.
M. B. Calvo (2009)
TTF-2/FOXE1 gene polymorphisms in Sicilian patients with permanent primary congenital hypothyroidism
L. Santarpia (2007)
Gene transfer of the Runx2 transcription factor enhances osteogenic activity of bone marrow stromal cells in vitro and in vivo.
Z. Zhao (2005)
Mechanisms of bone repair and regeneration.
F. Deschaseaux (2009)
Differential growth factor control of bone formation through osteoprogenitor differentiation.
L. Chaudhary (2004)
Critical molecular switches involved in BMP-2-induced osteogenic differentiation of mesenchymal cells.
Hyun-Mo Ryoo (2006)
Three‐dimensional fibrous PLGA/HAp composite scaffold for BMP‐2 delivery
Hemin Nie (2008)
The effect of fibroblast growth factor-2 on the osteoinductive activity of recombinant human bone morphogenetic protein-2 in rat muscle.
K. Fujimura (2002)
Retention of multilineage differentiation potential of mesenchymal cells during proliferation in response to FGF.
S. Tsutsumi (2001)
Smad-mediated Transcription Is Required for Transforming Growth Factor-β1-induced p57Kip2 Proteolysis in Osteoblastic Cells*
S. Nishimori (2001)
Role of biochemical markers of bone remodeling in clinical practice.
V. Camozzi (2007)
Effect of local sequential VEGF and BMP-2 delivery on ectopic and orthotopic bone regeneration.
D. Kempen (2009)
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E. M. Giles (2017)
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A. Lee (2017)
FGF-2 targets sclerostin in bone and myostatin in skeletal muscle to mitigate the deleterious effects of glucocorticoid on musculoskeletal degradation.
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Biomaterials Act as Enhancers of Growth Factors in Bone Regeneration
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Y. Kawakami (2019)
Osteo-/odontogenic differentiation of BMP2 and VEGF gene-co-transfected human stem cells from apical papilla
Wen Zhang (2016)
Enhancing effects of basic fibroblast growth factor and fibronectin on osteoblast adhesion to bone scaffolds for bone tissue engineering through extracellular matrix-integrin pathway.
L. Feng (2017)
Synergistic effects of fibroblast growth factor-2 and bone morphogenetic protein-2 on bone induction
R. Song (2017)
Gene expression profiles induced by growth factors in in vitro cultured osteoblasts
A. Robubi (2014)
Effect of Local Basic Fibroblast Growth Factor and Vascular Endothelial Growth Factor on Subcutaneously Allotransplanted Ovarian Tissue in Ovariectomized Mice
Jiangman Gao (2015)
Development of PLGA-PEG-COOH and gelatin-based microparticles dual delivery system and E-beam sterilization effects for controlled release of BMP-2 and IGF-1.
Y. Bai (2020)
Comparison of Impregnated Bone Morphogenetic Protein-2 Release Kinetics from Biopolymer Scaffolds
A. V. Vasilyev (2019)
Effects of piezosurgery in accelerating the movement of orthodontic alveolar bone tooth of rats and the expression mechanism of BMP-2
J. Han (2016)
Applicability of human dental follicle cells to bone regeneration without dexamethasone: an in vivo pilot study.
K. Takahashi (2015)
Dissection the Osteogenic and Angiogenic Signal Pathways in Bone Development and Regeneration with Biochips
Malsawmhriatzuala Jeremy (2014)
Sustained knock down of PPARγ and bFGF presentation in collagen hydrogels promote MSC osteogenesis
Shan Shao (2015)
Isolation and characterization of the human immature osteoblast culture system from the alveolar bones of aged donors for bone regeneration therapy
M. Aino (2014)
Combinations of growth factors for human mesenchymal stem cell proliferation and osteogenic differentiation
Veronika Hefka Blahnova (2020)
Effects of vascular endothelial growth factor on osteoblasts around dental implants in vitro and in vivo.
M. Guang (2017)
Enhancement of tendon-to-bone healing after anterior cruciate ligament reconstruction using bone marrow-derived mesenchymal stem cells genetically modified with bFGF/BMP2
Biao Chen (2016)
Controlled delivery of icariin on small intestine submucosa for bone tissue engineering.
M. Li (2017)
Ectopic osteogenesis effect of antigen-extracted xenogeneic cancellous bone graft with chitosan/rhBMP-2/bFGF sequential sustained-release nanocapsules
Z. Li (2018)
Multifunctional fibrous scaffolds for bone regeneration with enhanced vascularization.
C. Wang (2019)
Paracrine effect of the bone morphogeneticprotein-2 at the experimental site on healing of the adjacent control site: a study in the rabbit calvarial defect model
J. Lee (2014)
Odontogenic differentiation of human dental pulp cells by calcium silicate materials stimulating via FGFR/ERK signaling pathway.
Chao-Hsin Liu (2014)
Bone Morphogenetic Protein‐2 and Vascular Endothelial Growth Factor in Bone Tissue Regeneration: New Insight and Perspectives
R. Aryal (2014)
Bone Tissue Engineering Drug Delivery
P. F. Costa (2015)
Sequential application of bFGF and BMP-2 facilitates osteogenic differentiation of human periodontal ligament stem cells.
Wenyan Kang (2019)
New pathway of icariin-induced MSC osteogenesis: transcriptional activation of TAZ/Runx2 by PI3K/Akt
Guoying Zhang (2017)
Reconstructing jaw defects with MSCs and PLGA-encapsulated growth factors.
B. C. Tee (2016)
VEGF promotes osteogenic differentiation of ASCs on ordered fluorapatite surfaces.
D. Clark (2015)
Ciliary IFT 80 regulates dental pulp stem cells differentiation by FGF / FGFR 1 and Hh / BMP 2 signaling
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