Online citations, reference lists, and bibliographies.
← Back to Search

Hexavalent Chromate Reduction By Alkaliphilic Amphibacillus Sp. KSUCr3 Is Mediated By Copper-dependent Membrane-associated Cr(VI) Reductase

A. S. Ibrahim, M. A. El-Tayeb, Yahya B. Elbadawi, A. Al-Salamah, G. Antranikian
Published 2012 · Chemistry, Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
The present study was aimed to localize and characterize hexavalent chromate [Cr(VI)] reductase activity of the extreme alkaliphilic Amphibacillus sp. KSUCr3 (optimal growth pH 10.5). The resting cells were able to reduce about 62 % of the toxic heavy metal Cr(VI) at initial concentration of 200 μM within 30 min. Cell permeabilization resulted in decrease of Cr(VI) reduction in comparison to untreated cells. Enzymatic assays of different sub-cellular fractions of Amphibacillus sp. KSUCr3 demonstrated that the Cr(VI) reductase was mainly associated with the membranous fraction and expressed constitutively. In vitro studies of the crude enzyme indicated that copper ion was essential for Cr(VI) reductase activity. In addition, Ca2+ and Mn2+ slightly stimulated the chromate reductase activity. Glucose was the best external electron donor, showing enhancement of the enzyme activity by about 3.5-fold. The Km and Vmax determined for chromate reductase activity in the membranous fraction were 23.8 μM Cr(VI) and 72 μmol/min/mg of protein, respectively. Cr(VI) reductase activity was maximum at 40 °C and pH 7.0 and it was significantly inhibited in the presence of disulfide reducers (2-mercaptoethanol), ion chelating agent (EDTA), and respiratory inhibitors (CN and Azide). Complete reduction of 100 and 200 μM of Cr(VI) by membrane associated enzyme were observed within 40 and 180 min, respectively. However, it should be noted that biochemical characterization has been done with crude enzyme only, and that final conclusion can only be drawn with the purified enzyme.
This paper references
10.1099/00207713-41-1-190
Amphibacillus xylanus gen. nov., sp. nov., a Facultatively Anaerobic Sporeforming Xylan-Digesting Bacterium Which Lacks Cytochrome, Quinone, and Catalase
Y. Niimura (1990)
Chromium. In: DL Sparks (ed) Methods
RJ Bartlett (1996)
10.1007/s00253-003-1291-x
In vitro reduction of hexavalent chromium by a cell-free extract of Bacillus sp. ES 29 stimulated by Cu2+
F. Camargo (2003)
Extremophiles handbook, vol. 1–2 Isolation and characterization of novel potent Cr(VI) reducing alkaliphilic Amphibacillus sp. strain KSUCr3 from hypersaline soda lakes
K Horikoshi (2011)
10.1016/J.BIORTECH.2007.08.059
Comparison of in vitro Cr(VI) reduction by CFEs of chromate resistant bacteria isolated from chromate contaminated soil.
A. Sarangi (2008)
10.1128/AEM.66.5.1788-1795.2000
Purification to Homogeneity and Characterization of a Novel Pseudomonas putida Chromate Reductase
C. H. Park (2000)
10.1002/CHIN.198720020
Chromium(III) Hydrolysis Constants and Solubility of Chromium(III) Hydroxide.
D. Rai (1987)
10.1007/s00253-007-0862-7
Chromate reduction by Burkholderia cepacia MCMB-821,isolated from the pristine habitat of alkaline crater lake
Revati Wani (2007)
10.2172/876715
Bioremediation of metals and radionuclides: What it is and How itWorks
J. McCullough (1999)
10.1128/AEM.00813-06
Global Molecular and Morphological Effects of 24-Hour Chromium(VI) Exposure on Shewanella oneidensis MR-1
K. Chourey (2006)
10.1128/AEM.56.7.2268-2270.1990
Chromium reduction in Pseudomonas putida.
Y. Ishibashi (1990)
10.1016/J.PROCBIO.2006.01.006
Hexavalent chromium reduction by Providencia sp
Urvashi Thacker (2006)
10.1128/JB.172.3.1670-1672.1990
Membrane-associated chromate reductase activity from Enterobacter cloacae.
P. C. Wang (1990)
10.1007/s00284-002-3800-z
Characterization of Cr(VI)-Resistant Bacteria Isolated from Chromium-Contaminated Soil by Tannery Activity
C. Viti (2003)
10.1007/s13205-011-0038-0
Reduction of hexavalent chromium by Ochrobactrum intermedium BCR400 isolated from a chromium-contaminated soil
B. Kavita (2011)
10.1016/J.BIORTECH.2005.12.006
Microbial and plant derived biomass for removal of heavy metals from wastewater.
Sarabjeet Singh Ahluwalia (2007)
10.1128/JB.01766-07
A novel chromate reductase from Thermus scotoductus SA-01 related to old yellow enzyme.
D. Opperman (2008)
10.1007/s10529-005-5526-z
Reduction of Cr(VI) by a Bacillus sp.
R. Elangovan (2005)
10.1016/j.jhazmat.2010.09.072
Characterization and genomic analysis of a highly chromate resistant and reducing bacterial strain Lysinibacillus fusiformis ZC1.
Minyan He (2011)
10.1007/s00284-005-0048-4
Reduction of Hexavalent Chromium by Cell-Free Extract of Bacillus sphaericus AND 303 Isolated from Serpentine Soil
Arundhati Pal (2005)
Amphibacillus sediminis sp. nov., an endosporeforming bacterium
SY An (2007)
10.33073/PJM-2010-029
Chromate reduction by cell-free extract of Bacillus firmus KUCr1.
Gopi Ballav Sau (2010)
10.1128/AEM.67.3.1076-1084.2001
Chromate Reduction by a Pseudomonad Isolated from a Site Contaminated with Chromated Copper Arsenate
J. McLean (2001)
10.1016/j.biortech.2009.06.001
Evaluation of chromium(VI) removal behaviour by two isolates of Synechocystis sp. in terms of exopolysaccharide (EPS) production and monomer composition.
Sahlan Ozturk (2009)
10.1007/s12088-011-0095-4
Isolation and Characterization of Chromium(VI)-Reducing Bacteria from Tannery Effluents
M. Ilias (2011)
A rapid and sensitive method for quantitation
MM Bradford (1976)
10.1111/J.1574-6968.2006.00386.X
Membrane topology of the chromate transporter ChrA of Pseudomonas aeruginosa.
Rafael Jiménez-Mejía (2006)
10.1016/j.jhazmat.2008.07.041
Isolation and characterization of a Cr(VI)-reduction Ochrobactrum sp. strain CSCr-3 from chromium landfill.
Zhiguo He (2009)
Global molecular and morphological effects
SD (2006)
10.1016/j.biortech.2007.12.046
Evaluation of in vitro Cr(VI) reduction potential in cytosolic extracts of three indigenous Bacillus sp. isolated from Cr(VI) polluted industrial landfill.
Chirayu Desai (2008)
Purification and characterization of NADPH-dependent Cr(VI) reductase from Escherichia coli ATCC 33456.
Woo-Chul Bae (2005)
10.1046/j.1365-2672.2000.00910.x
Chromium(VI) reductase activity is associated with the cytoplasmic membrane of anaerobically grown Shewanella putrefaciens MR‐1
C. Myers (2000)
10.1080/10408440500534032
Toxicity and Carcinogenicity of Chromium Compounds in Humans
M. Costa (2006)
10.1016/j.sjbs.2010.12.003
Bioreduction of Cr(VI) by alkaliphilic Bacillus subtilis and interaction of the membrane groups.
M. S. Mary Mangaiyarkarasi (2011)
Membrane-Associated Hexavalent Chromium Reductase of Bacillus megaterium TKW3 with Induced Expression
K. H. Cheung (2006)
10.1080/10408440600932169
Response to Comments by Post and Stern on Article “Toxicity and Carcinogenicity of Chromium Compounds in Humans”
M. Costa (2006)
10.1021/ES048967G
Formation of soluble organo-chromium(III) complexes after chromate reduction in the presence of cellular organics.
G. Puzon (2005)
10.1016/S1001-0742(08)62346-3
Isolation of Cr(VI) reducing bacteria from industrial effluents and their potential use in bioremediation of chromium containing wastewater.
A. Zahoor (2009)
Hexavalent chromate
C Desai (2008)
10.1099/IJS.0.64926-0
Amphibacillus sediminis sp. nov., an endospore-forming bacterium isolated from lake sediment in Japan.
Sun-Young An (2007)
10.1007/s12010-011-9481-y
In Vitro Reduction of Hexavalent Chromium by Cytoplasmic Fractions of Pannonibacter phragmitetus LSSE-09 under Aerobic and Anaerobic Conditions
L. Xu (2011)
10.2225/VOL14-ISSUE4-FULLTEXT-4
Isolation and characterization of novel potent Cr(VI) reducing alkaliphilic Amphibacillus sp. KSUCr3 from hypersaline soda lakes
A. S. Ibrahim (2011)
Membrane-associated hexavalent
KH Cheung (2006)
10.1016/J.BIORTECH.2005.12.025
Reduction of toxic hexavalent chromium by Ochrobactrum intermedium strain SDCr-5 stimulated by heavy metals.
S. Sultan (2007)
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
10.1016/J.PROCBIO.2008.02.015
Hexavalent chromate reductase activity in cytosolic fractions of Pseudomonas sp. G1DM21 isolated from Cr(VI) contaminated industrial landfill
Chirayu Desai (2008)
Microbial and plant derived biomass
SS Ahluwalia (2007)
10.1007/s12011-008-8098-7
Environmental and Kinetic Parameters for Cr(VI) Bioreduction by a Bacterial Monoculture Purified from Cr(VI)-Resistant Consortium
B. Okeke (2008)
10.1128/MMBR.63.4.735-750.1999
Alkaliphiles: Some Applications of Their Products for Biotechnology
K. Horikoshi (1999)
10.1007/s00284-002-3889-0
Toxicity of Hexavalent Chromium and Its Reduction by Bacteria Isolated from Soil Contaminated with Tannery Waste
M. Megharaj (2003)
10.1128/AEM.59.11.3771-3777.1993
Characterization of enzymatic reduction of hexavalent chromium by Escherichia coli ATCC 33456.
H. Shen (1993)
10.1023/A:1013196017556
Amphibacillus fermentum sp. nov. and Amphibacillus tropicussp. nov., New Alkaliphilic, Facultatively Anaerobic, Saccharolytic Bacilli from Lake Magadi
T. Zhilina (2004)



This paper is referenced by
10.1016/j.jenvman.2013.04.011
Comparative evaluations on bio-treatment of hexavalent chromate by resting cells of Pseudochrobactrum sp. and Proteus sp. in wastewater.
Shimei Ge (2013)
Cr(VI) reduction by cell-free extract of thermophillic Bacillus fusiformis NTR9
P. Pattanapipitpaisal (2013)
10.1590/1678-4324-2017160394
Oxidative Stress, Chromium-Resistance and Uptake by Fungi: Isolated from Industrial Wastewater
A. Elahi (2018)
10.1007/978-981-15-1812-6
Microbial Bioremediation & Biodegradation
M. Shah (2020)
10.1016/j.ecoenv.2019.109792
Mechanisms of Cr(VI) reduction by Bacillus sp. CRB-1, a novel Cr(VI)-reducing bacterium isolated from tannery activated sludge.
Y. Zhu (2019)
10.1007/978-981-15-1812-6_9
Extremophiles: A Powerful Choice for Bioremediation of Toxic Oxyanions
Sana Alavi (2020)
10.1007/s12223-014-0304-8
Bacterial mechanisms for Cr(VI) resistance and reduction: an overview and recent advances
M. Ahemad (2014)
10.1016/j.chemosphere.2017.03.074
Chromium speciation, bioavailability, uptake, toxicity and detoxification in soil-plant system: A review.
Muhammad Shahid (2017)
10.1016/J.IBIOD.2014.03.015
Cr(VI) reduction by Enterobacter sp. DU17 isolated from the tannery waste dump site and characterization of the bacterium and the Cr(VI) reductase
Zeeshanur Rahman (2014)
10.1007/s12257-013-0373-6
Detoxification of hexavalent chromate by Amphibacillus sp. KSUCr3 cells immobilised in silica-coated magnetic alginate beads
A. S. Ibrahim (2013)
10.1016/J.BEJ.2016.09.001
Rapid start-up of the anammox process by denitrifying granular sludge and the mechanism of the anammox electron transport chain
Sihui Wang (2016)
10.1007/s00792-015-0733-6
Bioreduction and immobilization of hexavalent chromium by the extremely acidophilic Fe(III)-reducing bacterium Acidocella aromatica strain PFBC
Yusei Masaki (2015)
10.1134/S0006297914130094
Structural and functional studies of multiheme cytochromes c involved in extracellular electron transport in bacterial dissimilatory metal reduction
T. Tikhonova (2014)
10.1080/01490451.2014.971200
Chromate Interaction with the Chromate Reducing Actinobacterium Intrasporangium chromatireducens Q5-1
H. Liu (2015)
10.1007/s00253-012-4642-7
Radiation-resistant extremophiles and their potential in biotechnology and therapeutics
P. Gabani (2012)
10.1016/j.jenvman.2015.04.031
Simultaneous chromate reduction and azo dye decolourization by Lactobacillus paracase CL1107 isolated from deep sea sediment.
Guangdao Huang (2015)
10.5539/IJB.V6N2P64
Enzymatic Chromium (VI) Reduction by Cytoplasmic and Cell Membrane Fractions of Chromate-Reducing Bacterium Isolated From Sewage Treatment Plant
Paul Fabrice Nguema (2014)
10.3389/fbioe.2020.00521
New Plant Growth-Promoting, Chromium-Detoxifying Microbacterium Species Isolated From a Tannery Wastewater: Performance and Genomic Insights
R. Ouertani (2020)
10.3967/bes2015.014
Bioremediation of Hexavalent Chromium Pollution by Sporosarcina saromensis M52 Isolated from Offshore Sediments in Xiamen, China.
R. Zhao (2016)
10.1016/j.jenvman.2015.04.011
Bioremediation of hexavalent chromate using permeabilized Brevibacterium sp. and Stenotrophomonas sp. cells.
Shimei Ge (2015)
10.1016/J.JECE.2013.10.012
Stability of biologically reduced chromium in soil
Angela R. Bielefeldt (2014)
10.5539/IJB.V9N3P1
Treatment of Chromium Pollution by the Reductase Enzyme Generated by a Chromium-Resistant Bacterium Isolated in Dewatering Sludge
Paul Fabrice Nguema (2017)
10.1080/13102818.2014.937092
Hexavalent chromium reduction by chromate-resistant haloalkaliphilic Halomonas sp. M-Cr newly isolated from tannery effluent
Mona E. M. Mabrouk (2014)
10.1093/femsle/fnw146
Heavy metal resistance in halophilic Bacteria and Archaea.
Doriana Mădălina Voica (2016)
Semantic Scholar Logo Some data provided by SemanticScholar