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Influence Of Lactobacillus Fermentum I5007 On The Intestinal And Systemic Immune Responses Of Healthy And E. Coli Challenged Piglets
A. Wang, H. Yu, X. Gao, X. Li, S. Qiao
Published 2009 · Medicine, Biology
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The effect of feeding Lactobacillus fermentum I5007 on the immune system of weaned pigs with or without E. coli challenge was determined. Twenty-four weaned barrows (6.07 ± 0.63 kg BW) were randomly assigned to one of four treatments (N = 6) in a factorial design experiment. The first two treatments consisted of healthy piglets with half of the pigs receiving no treatment while the other half was orally administered with L. fermentum I5007 (108 CFU/ml) at a daily dose of 20 ml. Pigs in the second two treatments were challenged on the first day with 20 ml of E. coli K88ac (108 CFU/ml). Half of these pigs were not treated while the remaining pigs were treated with 20 ml of L. fermentum I5007 (108 CFU/ml). Peripheral blood lymphocytes subsets were determined using flow cytometry. The intestinal mucosal immunity of the pigs was monitored by real time polymerase chain reaction. The cytokine content of the pig’s serum was also analyzed. Oral administration of L. fermentum I5007 increased blood CD4+ lymphocyte subset percentage as well as tumor necrosis factor-α and interferon-γ expression in the ileum. Pigs challenged with E. coli had elevated jejunal tumor necrosis factor-α while interferon-γ expression was increased throughout the small intestine. There was no difference in the concentration of the cytokines interleukin-2, interleukin-6, tumor necrosis factor-α and interferon-γ in the serum. CD8+ and CD4+/CD8+ in peripheral blood were not affected by treatment. In conclusion, L. fermentum I5007 can enhance T cell differentiation and induce ileum cytokine expression suggesting that this probiotic strain could modulate immune function in piglets.
This paper references
Th 1 / Th 2 Balance : The Hypothesis , its Limitations , and Implications for Health and Disease
P. Kidd (2003)
Effects of bifidobacterial cytoplasm, cell wall and exopolysaccharide on mouse lymphocyte proliferation and cytokine production
Tahar Amrouche (2006)
Th1/Th2 balance: the hypothesis, its limitations, and implications for health and disease.
P. Kidd (2003)
Selection of potential probiotic lactobacilli from pig feces to be used as additives in pelleted feeding.
M. D. De Angelis (2006)
Enterotoxigenic Escherichia coli (ETEC) in farm animals.
B. Nagy (1999)
Effect of viable
HF Yu (2008)
The adhesion of putative probiotic lactobacilli to cultured epithelial cells and porcine intestinal mucus
X. Li (2008)
Effect of long-term continuous consumption of fermented milk containing probiotic bacteria on mucosal immunity and the activity of peritoneal macrophages.
A. de Moreno de LeBlanc (2008)
Does the microbiota regulate immune responses outside the gut?
M. C. Noverr (2004)
Functional modulation of enterocytes by gram-positive and gram-negative microorganisms.
J. Otte (2004)
Cellular and Molecular Immunology
A. Abbas (1991)
Anti-inflammatory potential of the probiotic dietary supplement Lactibiane Tolérance: in vitro and in vivo considerations.
Sophie Drouault-Holowacz (2006)
Oral tolerance and its role in clinical disease.
G. Spiekermann (2001)
CD 4 and CD 8 : modulators of T-cell receptor recognition of antigen and of immune responses ?
R. Zamoyska (2001)
Lactobacilli and Streptococci Induce Interleukin-12 (IL-12), IL-18, and Gamma Interferon Production in Human Peripheral Blood Mononuclear Cells
M. Miettinen (1998)
Host responses to Renibacterium salmoninarum and specific components of the pathogen reveal the mechanisms of immune suppression and activation
T. Hilton Grayson (2002)
The gills are an important site of iNOS expression in rainbow trout Oncorhynchus mykiss after challenge with the Gram‐positive pathogen Renibacterium salmoninarum
J. J. Campos-Pérez (2000)
Protec - tion of just weaned pigs against infection with F 18 t Escherichia coli by nonimmune plasma powder
H Nollet (1999)
The effect of dietary spray-dried porcine plasma on clinical response in weaned piglets challenged with a pathogenic Escherichia coli.
A. V. van Dijk (2002)
Protection of just weaned pigs against infection with F18+ Escherichia coli by non-immune plasma powder.
H. Nollet (1999)
Probiotics and non-intestinal infectious conditions.
M. de Vrese (2002)
Effects of Lactobacilli on the Performance, Diarrhea Incidence, VFA Concentration and Gastrointestinal Microbial Flora of Weaning Pigs
C. Huang (2004)
The effect of oral feeding of Lactobacillus casei strain Shirota on immunoglobulin E production in mice.
T. Matsuzaki (1998)
JM Otte (2004)
Enteric immunization reveals a T cell network for IgA responses and suggests that humans possess a common mucosal immune system
J. McGhee (2004)
The gastrointestinal ecosystem: a precarious alliance among epithelium, immunity and microbiota
V. J. McCracken (2001)
Pathologic and physiologic interactions of bacteria with the gastrointestinal epithelium.
L. Lu (2001)
Probiotic alternatives to reduce gastrointestinal infections: the poultry experience.
G. Nava (2005)
Enhancement of natural and acquired immunity by Lactobacillus rhamnosus (HN001), Lactobacillus acidophilus (HN017) and Bifidobacterium lactis (HN019).
H. Gill (2000)
Two Lactobacillus strains, isolated from breast milk, differently modulate the immune response
M. P. Díaz-Ropero (2007)
Evaluation of immunomodulation by Lactobacillus casei Shirota: immune function, autoimmunity and gene expression.
K. Baken (2006)
Influences of microbiota on intestinal immune system development.
J. Cebra (1999)
Effect of extracorporeal liver support
C Lackner (2006)
The effect of dietary spraydried porcine plasma on clinical response in weaned piglets challenged with a pathogenic Escherichia coli
AJ VanDijk (2002)
CD4 and CD8: modulators of T-cell receptor recognition of antigen and of immune responses?
R. Zamoyska (1998)
Inflammatory Immune Response
A. de Moreno de LeBlanc (2004)
Immune enhancing effects of Lactobacillus acidophilus LAFTI L10 and Lactobacillus paracasei LAFTI L26 in mice.
G. Paturi (2007)
Roles of CD4 T cells
T Mikami (1999)
XJ Li (2008)
Activation of Human Peripheral Blood Mononuclear Cells by Nonpathogenic Bacteria In Vitro: Evidence of NK Cells as Primary Targets
D. Haller (2000)
Immune modulation of blood leukocytes in humans by lactic acid bacteria: criteria for strain selection.
E. Schiffrin (1997)
Expression of immune response genes in rainbow trout skin induced by Gyrodactylus derjavini infections.
T. Lindenstrøm (2004)
Interaction of lactic acid bacteria with the gut immune system
G. Perdigón (2002)
Effect of extracorporeal liver support by MARS and Prometheus on serum cytokines in acute-on-chronic liver failure
V. Stadlbauer (2006)
Lactobacillus casei reduces CD8+ T cell‐mediated skin inflammation
L. Chapat (2004)
Regulatory effect of yoghurt on intestinal inflammatory immune response
A de Moreno de LeBlanc (2004)
The Probiotic Bacterium Lactobacillus casei Induces Activation of the Gut Mucosal Immune System through Innate Immunity
C. M. Galdeano (2006)
Roles of CD4+ T Cells and Gamma Interferon in Protective Immunity against Babesia microtiInfection in Mice
I. Igarashi (1999)
Effect of viable Lactobacillus fermentum on the growth performance, nutrient digestibility and immunity of weaned pigs
H. Yu (2008)
Conjugated linoleic acid attenuates the production and gene expression of proinflammatory cytokines in weaned pigs challenged with lipopolysaccharide.
Lai Chang-hua (2005)
Developmental microbial ecology of the neonatal gastrointestinal tract.
R. Mackie (1999)
ETEC) in animals
GM Nava (2005)
ulatory effect of yoghurt on intestinal inflammatory immune response
A deMorenodeLeBlanc (2004)
Type 1 and type 2: a fundamental dichotomy for all T-cell subsets.
L. Carter (1996)
The indigenous gastrointestinal microflora.
R. Berg (1996)
This paper is referenced by
Construction and validation of a mCherry protein vector for promoter analysis in 1 Lactobacillus acidophilus 2
M. L. Mohedano (2014)
Lactobacillus reuteri HCM2 protects mice against Enterotoxigenic Escherichia coli through modulation of gut microbiota
T. Wang (2018)
Construction and validation of a mCherry protein vector for promoter analysis in Lactobacillus acidophilus
M. L. Mohedano (2014)
Evaluation of Weissella Cibaria JW15 Probiotic Derived from Fermented Korean Vegetable Product Supplementation in Diet on Performance Characteristics in Adult Beagle Dog
H. Y. Sun (2019)
Probiotics—Live Biotherapeutics: a Story of Success, Limitations, and Future Prospects—Not Only for Humans
Mohamed Zommiti (2019)
The Potential Role of Probiotics (nutraceuticals) in Gut Health of Domestic Animals; an Alternative to Antibiotic Growth Promoters
A. Nawab (2019)
Lactobacillus fermentum and its potential immunomodulatory properties
Y. Zhao (2019)
Probiotics - do they have a role in the pig industry?
M. Kenny (2011)
The potential of probiotics as in-feed growth enhancers for swine
A. Wealleans (2010)
Probiotics and Prebiotics for the Health of Pigs and Horses
S. Kritas (2018)
Combined dietary supplementation of long chain inulin and Lactobacillus acidophilus W37 supports oral vaccination efficacy against Salmonella Typhimurium in piglets
Alexia F. P. Lépine (2019)
Dietary Supplementation with Probiotic Lactobacillus fermentum I5007 and the Antibiotic, Aureomycin, Differentially Affects the Small Intestinal Proteomes
X. Wang (2011)
Study and use of the probiotic Lactobacillus reuteri in pigs: a review
C. Hou (2015)
Effect of ultrafine pulverization of Senecio scandens on growth, immune system and faecal microorganisms in piglets.
Jia-jing Yue (2016)
Dietary supplementation with the probiotic Lactobacillus fermentum I5007 and the antibiotic aureomycin differentially affects the small intestinal proteomes of weanling piglets.
X. Wang (2012)
Small intestinal segment perfusion test in piglets: future applications in studying probiotics-gut crosstalk in infectious diarrhoea?
Jan van der Meulen (2010)
Superoxide dismutase recombinant Lactobacillus fermentum ameliorates intestinal oxidative stress through inhibiting NF‐κB activation in a trinitrobenzene sulphonic acid‐induced colitis mouse model
C. L. Hou (2014)
Expression of catalase in Lactobacillus fermentum and evaluation of its anti-oxidative properties in a dextran sodium sulfate induced mouse colitis model
Jiang Zhang (2013)
Efeito de probiótico no desenvolvimento de leitões desafiados com Escherichia coli
Edison de Almeida (2012)
Practical aspects of the use of probiotics in pig production: A review
E. Barba-Vidal (2019)
The effect of Lactobacillus on the expression of porcine β-defensin-2 in the digestive tract of piglets
J. Zhang (2011)
Genomics of Probiotic–Host Interactions
D. Ajithdoss (2012)
Probiotics: From Isolation to Application
P. Shokryazdan (2017)
A feed additive containing Bacillus toyonensis (Toyocerin®) protects against enteric pathogens in postweaning piglets
D. Kantas (2015)
In Search of Health-Promoting Microbes: In Vitro and In Vivo Studies in Swine
Tanja Lähteinen (2014)
Fermented Whey Permeate for Piglets: As a strategy to reduce Post Weaning Diarrhoea
Sarmauli I. Manurung (2012)
Lactobacillus fermentum JX306 Restrain D-galactose-induced Oxidative Stress of Mice through its Antioxidant Activity
D. Zhang (2020)
Probiotics as a Dietary Additive for Pigs: A Review
J. H. Cho (2011)
Effects of Newly Developed Synbiotic and Commercial Probiotic Products on The Haematological Indices, Serum Cytokines, Acute Phase Proteins Concentration, and Serum Immunoglobulins Amount in Sows and Growing Pigs – A Pilot Study
E. Czyżewska-Dors (2018)
In vitro differential modulation of immune response by probiotics in porcine peripheral blood mononuclear cells
Verónica Mata-Haro (2014)
Effect of swine based probiotic on performance, diarrhoea scores, intestinal microbiota and gut health of grower-finisher crossbred pigs
Runjun Dowarah (2017)
Heritability and genome-wide association of swine gut microbiome features with growth and fatness parameters
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