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Improved Adherence And Spreading Of Saos-2 Cells On Polypropylene Surfaces Achieved By Surface Texturing And Carbon Nitride Coating

Katja Myllymaa, S. Myllymaa, H. Korhonen, M. Lammi, Hanna Saarenpää, Mika Suvanto, T. Pakkanen, V. Tiitu, R. Lappalainen
Published 2009 · Medicine, Materials Science

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The adhesion and contact guidance of human primary osteogenic sarcoma cells (Saos-2) were characterized on smooth, microstructured (MST) and micro- and nano-structured (MNST) polypropylene (PP) and on the same samples with a silicon-doped carbon nitride (C3N4-Si) coating. Injection molding was used to pattern the PP surfaces and the coating was obtained by using ultra-short pulsed laser deposition (USPLD). Surfaces were characterized using atomic force microscopy and surface energy components were calculated according to the Owens-Wendt model. The results showed C3N4-Si coated surfaces to be significantly more hydrophilic than uncoated ones. In addition, there were 86% more cells in the smooth C3N4-Si coated PP compared to smooth uncoated PP and 551%/476% more cells with MST/MNST C3N4-Si coated PP than could be obtained with MST/MNST uncoated PP. Thus the adhesion, spreading and contact guidance of osteoblast-like cells was effectively improved by combining surface texturing and deposition of osteocompatible C3N4-Si coating.
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