Synthesis Of Selenium Nanoparticles Using Probiotic Bacteria Lactobacillus Acidophilus And Their Enhanced Antimicrobial Activity Against Resistant Bacteria
Hammad Alam, Nafeesa Khatoon, Mohammad Aasif Khan, Syed Akhtar Husain, Muthupandian Saravanan, Meryam Sardar
Published 2019 · Chemistry
Download PDFAnalyze on Scholarcy
In the present study, nontoxic selenium nanoparticles were synthesized extracellularly using probiotic bacteria Lactobacillus acidophilus. The synthesized Lactobacillus acidophilus mediated selenium nanoparticles (LA-SeNPs) show the surface plasmon resonance (SPR) at 385 nm. The hydrodynamic radius of LA-SeNPs was found to be 34.13 nm along with polydispersity index (0.28) and zeta potential (+ 37.86 mV). TEM shows that the average diameter of LA-SeNPs is 2–15 nm. FTIR suggest that extracellular proteins present in bacterial culture were responsible for reduction and stabilization of Se ions to LA-SeNPs. The antibacterial activity of synthesized nanoparticles was studied against five different sensitive and resistant bacterial strains. The MIC90 for bacterial strains were in the range ± 1 to ± 10 µg/ml. The inhibition and degradation of bacterial biofilm were studied against all the tested strains. The synthesized nanoparticles were cyto-compatible against human HEK-293 normal cell lines shown by MTT assay.
This paper references
Biosynthesis and structural characteristics of selenium nanoparticles by Pseudomonas alcaliphila.
Wenjie Zhang (2011)
L. Wang (1227)
Production of selenium nanoparticles in Pseudomonas putida KT2440
Roberto Avendaño (2016)
M. Kechagia (2013)
Approved standard. National Committee for Clinical Laboratory Standards Antimicrobial Susceptibility Testing NCCLS-M7
H J. (1993)
C. Xu (1632)
Synthesis and Characterization of Nano Selenium Using Plant Biomolecules and Their Potential Applications
Hammad Alam (2019)
RpoS- and OxyR-independent induction of HPI catalase at stationary phase in Escherichia coli and identification of rpoS mutations in common laboratory strains.
J E Visick (1997)
G. M. Khiralla (1001)
Nano-selenium and its nanomedicine applications: a critical review
B. Hosnedlova (2018)
Metal nanoparticles: understanding the mechanisms behind antibacterial activity
Yael N. Slavin (2017)
Thymoquinone inhibits biofilm formation and has selective antibacterial activity due to ROS generation
Surbhi Goel (2018)
Ultrastructural Characterization of the Lower Motor System in a Mouse Model of Krabbe Disease
V. Cappello (2016)
A spectrophotometric assay for superoxide dismutase activities in crude tissue fractions.
H. Kuthan (1986)
Health Benefits of Probiotics: A Review
Maria Kechagia (2013)
Preparation, characteristics and antioxidant activity of polysaccharides and proteins-capped selenium nanoparticles synthesized by Lactobacillus casei ATCC 393.
Chunlan Xu (2018)
M. Mallick (2015)
Synthesis and characterization of biogenic selenium nanoparticles with antimicrobial properties made by Staphylococcus aureus, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, and Pseudomonas aeruginosa.
David Medina Cruz (2018)
Biosynthesis of Polysaccharides-Capped Selenium Nanoparticles Using Lactococcus lactis NZ9000 and Their Antioxidant and Anti-inflammatory Activities
Chunlan Xu (2019)
Rapid biosynthesis of silver nanoparticles from Bacillus megaterium (NCIM 2326) and their antibacterial activity on multi drug resistant clinical pathogens.
Msp Saravanan (2011)
Investigation of functional selenium nanoparticles as potent antimicrobial agents against superbugs.
Xiaoquan Huang (2016)
третьему Совещанию (2017)
Biogenic Synthesis of Novel Functionalized Selenium Nanoparticles by Lactobacillus casei ATCC 393 and Its Protective Effects on Intestinal Barrier Dysfunction Caused by Enterotoxigenic Escherichia coli K88
Chunlan Xu (2018)
Biosynthesis of selenium nanoparticles by Pantoea agglomerans and their antioxidant activity
Slendy Katerine Andrade Torres (2012)
Bacillus cereus, selenite-reducing bacterium from contaminated lake of an industrial area: a renewable nanofactory for the synthesis of selenium nanoparticles
Aruna Jyothi Kora (2018)
Antimicrobial and antibiofilm effects of selenium nanoparticles on some foodborne pathogens
Ghada M Khiralla (2015)
Synthesis of silver nanoparticles from Bacillus brevis (NCIM 2533) and their antibacterial activity against pathogenic bacteria.
Muthupandian Saravanan (2018)
P. Mishra (1993)
Ashish Kumar (1824)
Probiotics, prebiotics and synbiotics- a review
Kavita R. Pandey (2015)
Extracellular biosynthesis and transformation of selenium nanoparticles and application in H2O2 biosensor.
Tingting Wang (2010)
Fig. 5 a Growth inhibition of LA-SeNPs on cancer cell lines (HEK293). All the values represent the mean ± SD of the experiment, performed in triplicate H
Y. N. Slavin (2017)
In situ immune response and mechanisms of cell damage in central nervous system of fatal cases microcephaly by Zika virus
Raimunda S. S. Azevedo (2017)
HPTLC Analysis of Bioactivity Guided Anticancer Enriched Fraction of Hydroalcoholic Extract of Picrorhiza kurroa
Md. Nasar Mallick (2015)
A novel microfluidic wound model for testing antimicrobial agents against Staphylococcus pseudintermedius biofilms
Jacob Terry (2014)
Molecular Mechanisms of Antibacterial Multidrug Resistance
Michael N. Alekshun (2007)
Removal of Protein Capping Enhances the Antibacterial Efficiency of Biosynthesized Silver Nanoparticles
Navin Kumar Jain (2015)
Corrigendum: The Serum Profile of Hypercytokinemia Factors Identified in H7N9-Infected Patients can Predict Fatal Outcomes
J. Guo (2016)
miR-500a-5p regulates oxidative stress response genes in breast cancer and predicts cancer survival
D. Degli Esposti (2017)
CD11c+ CD8+ T Cells Reduce Renal Fibrosis Following Ureteric Obstruction by Inducing Fibroblast Apoptosis
Haidong Wang (2016)
Speeding up bioproduction of selenium nanoparticles by using Vibrio natriegens as microbial factory
Helga Fernández-Llamosas (2017)
Biogenic selenium nanoparticles: characterization, antimicrobial activity and effects on human dendritic cells and fibroblasts
Eleonora Cremonini (2016)
Novel mechanisms of selenate and selenite reduction in the obligate aerobic bacterium Comamonas testosteroni S44.
Yuanqing Tan (2018)
Inhibition of Biofilm Formation, Quorum Sensing and Infection in Pseudomonas aeruginosa by Natural Products-Inspired Organosulfur Compounds
Nathaniel C. Cady (2012)
Biosynthesis of selenium-nanoparticles and -nanorods as a product of selenite bioconversion by the aerobic bacterium Rhodococcus aetherivorans BCP1.
Alessandro Presentato (2018)
M. N. Alekshunand (1037)
Genome-wide association study of paclitaxel and carboplatin disposition in women with epithelial ovarian cancer
Bo Gao (2018)
Nanobiotechnol. 15, 1. Fig. 5 a Growth inhibition of LA-SeNPs on cancer cell lines (HEK293). All the values represent the mean ± SD of the experiment, performed in triplicate
H. Alam (2017)
Green and Sustainable Selenium Nanoparticles and Their Biotechnological Applications
Meryam Sardar (2018)
Green and sustainable selenium nanoparticles and their biotechnological applications. Green and Sustainable Advanced Materials: Processing and Characterization
M. Sardar (2018)
Biosynthesis of selenium nanoparticles using Enterococcus faecalis and evaluation of their antibacterial activities.
Sara Shoeibi (2017)
All the values represent the mean ± SD of the experiment
Synthesis of silver nanoparticles from Phenerochaete chrysosporium (MTCC-787) and their antibacterial activity against human pathogenic bacteria.
Muthupandian Saravanan (2018)
Estimating the Impact of Workplace Bullying: Humanistic and Economic Burden among Workers with Chronic Medical Conditions
A. Fattori (2015)
The antimicrobial activity of nanoparticles: present situation and prospects for the future
L. Wang (2017)
This paper is referenced by
Biogenesis of selenium nanoparticles and their anti-leukemia activity
Kasi Anu (2020)