Synthesis Optimization And Characterization Of Chitosan-coated Iron Oxide Nanoparticles Produced For Biomedical Applications
Gozde Unsoy, Serap Yalçın, Rouhollah Khodadust, Gungor Gunduz, Ufuk Gunduz
Published 2012 · Materials Science
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The chitosan-coated magnetic nanoparticles (CS MNPs) were in situ synthesized by cross-linking method. In this method; during the adsorption of cationic chitosan molecules onto the surface of anionic magnetic nanoparticles (MNPs) with electrostatic interactions, tripolyphosphate (TPP) is added for ionic cross-linking of the chitosan molecules with each other. The characterization of synthesized nanoparticles was performed by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS/ESCA), Fourier transform infrared spectroscopy (FTIR), transmission electron microscopy (TEM), dynamic light scattering (DLS), thermal gravimetric analysis (TGA), and vibrating sample magnetometry (VSM) analyses. The XRD and XPS analyses proved that the synthesized iron oxide was magnetite (Fe3O4). The layer of chitosan on the magnetite surface was confirmed by FTIR. TEM results demonstrated a spherical morphology. In the synthesis, at higher NH4OH concentrations, smaller sized nanoparticles were obtained. The average diameters were generally between 2 and 8 nm for CS MNPs in TEM and between 58 and 103 nm in DLS. The average diameters of bare MNPs were found as around 18 nm both in TEM and DLS. TGA results indicated that the chitosan content of CS MNPs were between 15 and 23 % by weight. Bare and CS MNPs were superparamagnetic. These nanoparticles were found non-cytotoxic on cancer cell lines (SiHa, HeLa). The synthesized MNPs have many potential applications in biomedicine including targeted drug delivery, magnetic resonance imaging (MRI), and magnetic hyperthermia.
This paper references
Synthesis and Magnetic Properties of Cobalt Ferrite Nanoparticles
Morad Etier (2012)
Control synthesis of magnetic
L Zhang (2010)
Formulation and characterization of amphotericin B-chitosan-dextran sulfate nanoparticles.
Waree Tiyaboonchai (2007)
functionalization of magnetite nanoparticles using an am - inophosphonic acid coupling agent : new , ultradispersed , iron - oxide folate nanoconjugates for cancer - specific targeting
EB Denkbas (2008)
Accumulation of 2-μm latex particles in mouse Peyer's patches during chronic latex feeding
Michelle Lefevre (2005)
Superparamagnetic Iron Oxide Nanoparticles: Diagnostic Magnetic Resonance Imaging and Potential Therapeutic Applications in Neurooncology and Central Nervous System Inflammatory Pathologies, a Review
Jason S Weinstein (2010)
Preparation and characterization of magnetic nanoparticles with chitosan coating
Doan Thi Kim Dung (2009)
Preparation and properties of magnetic Fe3O4–chitosan nanoparticles
Gui-yin Li (2008)
Nanoparticle Based Drug Delivery System: Advantages and Applications
Sandeep Kumar Singh (2011)
Shape Evolution and Tunable Properties of Monodisperse Magnetite Crystals Synthesized by a Facile Surfactant‐Free Hydrothermal Method
Rongzheng Liu (2010)
A study of particulate intestinal absorption and hepatocellular uptake. Use of polystyrene latex particles.
Erhard Sanders (1961)
Synthesis and magnetic properties of cobalt ferrite ( CoFe 2 O 4 ) nanoparticles prepared by wet chemical route
K Maaz (2007)
Studies on effect of pH on cross-linking of chitosan with sodium tripolyphosphate: A technical note
Devika R. Bhumkar (2008)
Recent advances on chitosan-based micro- and nanoparticles in drug delivery.
Sunil A. Agnihotri (2004)
Chitosan-Based Particles as Controlled Drug Delivery Systems
Mani Prabaharan (2005)
Nanomedicine for respiratory diseases.
Ulrich Pison (2006)
Hydrothermal synthesis of monodisperse magnetite nanoparticles
T Jean Daou (2006)
Targeted delivery of low molecular drugs using chitosan and its derivatives.
Jae Hyung Park (2010)
Influence of tripolyphosphate cross-linking on the physical stability and lipase digestibility of chitosan-coated lipid droplets.
Min Hu (2010)
netic chitosan microspheres : preparation and characterization
AT Florence (1995)
Preparation of hydrophilic magnetic nanospheres with high saturation magnetization
Hong Xu (2007)
Encyclopedia of analytical chemistry : applications, theory and instrumentation
Robert A. Meyers (2000)
Bleomycin Loaded Magnetic Chitosan Nanoparticles as Multifunctional Nanocarriers
Doga Kavaz (2010)
Platinum-maghemite core-shell nanoparticles using a sequential synthesis
Xiaowei Teng (2003)
Preparation and Characterization of Acylated Chitosan
Preparation and properties of magnetic Fe 3 O 4 – chitosan nanoparticles
Superparamagnetic iron oxide nanoparticles: diagnostic magnetic resonance imaging and potential therapeutic applications in neurooncology and central nervous system
JS Weinstein (2010)
John Harold Talbott (1975)
Degradation of covalently cross-linked carboxymethyl chitosan and its potential application for peripheral nerve regeneration
Guangyuan Lu (2007)
Emerging use of nanoparticles in diagnosis and treatment of breast cancer.
Maksym V. Yezhelyev (2006)
Control synthesis of magnetic Fe3O4–chitosan nanoparticles under UV irradiation in aqueous system
Lian-ying Zhang (2010)
Magnetic iron oxide nanoparticles: synthesis, stabilization, vectorization, physicochemical characterizations, and biological applications.
Sophie Laurent (2008)
Chitosan and Chitosan/Ethylene Oxide-Propylene Oxide Block Copolymer Nanoparticles as Novel Carriers for Proteins and Vaccines
Pilar Calvo (2004)
Infrared evolved gas analysis during thermal investigation of lanthanum, europium and samarium carbonates
Lilli Paama (2003)
In situ preparation of magnetic Fe3O4-chitosan nanoparticles for lipase immobilization by cross-linking and oxidation in aqueous solution.
Yue Wu (2009)
Synthesis, Characterization and Ionic Conductive Properties of Phosphorylated Chitosan Membranes
Ying Wan (2003)
Design and fabrication of magnetic nanoparticles for targeted drug delivery and imaging.
Omid Veiseh (2010)
Nanoparticles as carriers for oral peptide absorption: Studies on particle uptake and fate
Alexander T. Florence (1995)
Magnetic chitosan microspheres: preparation and characterization
Emir Baki Denkbaş (2002)
Preparation of a chitosan-coated C18-functionalized magnetite nanoparticle sorbent for extraction of phthalate ester compounds from environmental water samples
Xiao Le Zhang (2010)
Synthèse en milieu alcalin de magnétite colloïdale: contrôle du rendement et de la taille des particules
René Massart (1987)
Characteristics of equilibrium, kinetics studies for adsorption of fluoride on magnetic-chitosan particle.
Wei Ma (2007)
In situ preparation
Y Wu (2009)
Room temperature solvent-free synthesis of monodisperse magnetite nanocrystals.
Xiaoqin Ye (2006)
Interpretation of Infrared Spectra, A Practical Approach
John Coates (2006)
Preparation and characterization of magnetic chitosan nanoparticles and its application for Cu(II) removal
Chen Yu-wei (2011)
Magnetically labeled cells can be detected by MR imaging.
Ralph Weissleder (1997)
Magnetic nanoparticle design for medical diagnosis and therapy
Stéphane Mornet (2004)
Bio-functionalization of magnetite nanoparticles using an aminophosphonic acid coupling agent: new, ultradispersed, iron-oxide folate nanoconjugates for cancer-specific targeting.
Manasmita Das (2008)
Preparation and characterization of magnetic chitosan nanospheres
Doina Hritcu (2009)
Synthesis of ferrofluid with magnetic nanoparticles by sonochemical method for MRI contrast agent
Eun Hee Kim (2005)
N-hexanoyl chitosan stabilized magnetic nanoparticles: Implication for cellular labeling and magnetic resonance imaging
Shanta Raj Bhattarai (2008)
Surface-modified superparamagnetic nanoparticles for drug delivery: preparation, characterization, and cytotoxicity studies
A.K. Gupta (2004)
Superparamagnetic nanoparticles for biomedical applications: possibilities and limitations of a new drug delivery system
Tobias Neuberger (2005)
In Situ Mineralization of Magnetite Nanoparticles in Chitosan Hydrogel
Yongliang Wang (2009)
Drug Delivery Systems: Entering the Mainstream
Theresa M. Allen (2004)
Recent advances in iron oxide nanocrystal technology for medical imaging.
Claire Corot (2006)
This paper is referenced by
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Synthesis, characterization, applications, and challenges of iron oxide nanoparticles
Attarad Ali (2016)
Degradation of polycyclic aromatic hydrocarbons by free and nanoclay-immobilized manganese peroxidase from Anthracophyllum discolor.
Francisca Acevedo (2010)
In situ synthesis of Fe3O4 nanoparticles coated by chito-oligosaccharides: physico-chemical characterizations and cytotoxicity evaluation for biomedical applications.
Paula Nunes de Oliveira (2020)
Chitosan nanoparticles' functionality as redox active drugs through cytotoxicity, radical scavenging and cellular behaviour.
Sreelatha Sarangapani (2018)
Physicochemical and biological characteristics of the nanostructured polysaccharide-iron hydrogel produced by microorganism Klebsiella oxytoca.
Sedigheh Kianpour (2017)
Magnetic iron oxide nanoparticles for drug delivery: applications and characteristics
Thomas Vangijzegem (2019)
Effects of Photocatalytic Nanoparticle Interfaces on Biological Membranes and Biomacromolecules
Manoranjan Arakha (2017)
ROS-Responsive Chitosan Coated Magnetic Iron Oxide Nanoparticles as Potential Vehicles for Targeted Drug Delivery in Cancer Therapy
Srinivasan Ayyanaar (2020)
Green synthesis, formulation and biological evaluation of a novel ZnO nanocarrier loaded with paclitaxel as drug delivery system on MCF-7 cell line.
Maedeh Akbarian (2019)
Adsorptive removal of oil from water using SPIONs–chitosan nanocomposite: kinetics and process optimization
Harpreet Singh (2019)
A simple approach to design chitosan functionalized Fe3O4 nanoparticles for pH responsive delivery of doxorubicin for cancer therapy
Mahesh G Adimoolam (2018)
In-situ synthesis and characterization of biocompatible magnetic ginger nanofiber composites for copper (II) removal from water
Joby Jacob (2019)
Synthesis, characterization, cytotoxicity and DNA binding studies of Fe3O4@SiO2 nanoparticles coated by an antiviral drug lamivudine
Nahid Shahabadi (2018)
Polyhydroxybutyrate-coated magnetic nanoparticles for doxorubicin delivery: cytotoxic effect against doxorubicin-resistant breast cancer cell line.
Serap Yalçın (2014)
Zero-order controlled release of BMP2-derived peptide P24 from the chitosan scaffold by chemical grafting modification technique for promotion of osteogenesis in vitro and enhancement of bone repair in vivo
Yan Chen (2017)
Iron oxide nanoparticles: Diagnostic, therapeutic and theranostic applications
Seyed Mohammadali Dadfar (2019)
Folic acid functionalized PEG coated magnetic nanoparticles for targeting anti-cancer drug delivery: Preparation, characterization and cytotoxicity on Doxorubicin, Zoledronic acid and Paclitaxel resistant MCF-7 breast cancer cell lines
Tuğba Keskin (2018)
303 SYNTHESIS , CHARACTERIZATION AND SELECTED APPLICATION OF CHITOSAN-COATED MAGNETIC IRON OXIDE NANOPARTICLES
Mostafa Radwan (2019)
Targeted anti-cancer prodrug based on carbon nanotube with photodynamic therapeutic effect and pH-triggered drug release
Jianquan Fan (2013)
Boronate-affinity based magnetic molecularly imprinted nanoparticles for the efficient extraction of the model glycoprotein horseradish peroxidase
Xiao-yu Sun (2017)
IN SITU SYNTHESIS and CHARACTERIZATION OF CHITOSAN COATED IRON OXIDE NANOPARTICLES and LOADING OF DOXORUBICIN
Gozde Unsoy (2012)
Application of response surface methodology for optimization of lead removal from an aqueous solution by a novel superparamagnetic nanocomposite
Vahid Javanbakht (2017)
Türkiye’de 2008-2018 Döneminde Yapılan Biyomedikal Mühendisliğindeki Araştırmaların Derlemesi
Mehlika Karamanlioglu (2019)
Bacteria-assisted biogreen synthesis of radical scavenging exopolysaccharide-iron complexes: an oral nano-sized nutritional supplement with high in vivo compatibility.
Seyyed Vahid Niknezhad (2019)
PEGylation of SPIONs by polycondensation reactions: a new strategy to improve colloidal stability in biological media
Wesley Renato Viali (2013)
Human adipose–derived mesenchymal stem cell–based medical microrobot system for knee cartilage regeneration in vivo
Gwangjun Go (2020)
Nanobiomaterials’ applications in neurodegenerative diseases
Daniela Silva Adaya (2017)
The enzyme-sensitive release of prodigiosin grafted β-cyclodextrin and chitosan magnetic nanoparticles as an anticancer drug delivery system: Synthesis, characterization and cytotoxicity studies.
Banafsheh Rastegari (2017)
Chitosan-Coated Magnetic Nanoparticles Prepared in One-Step by Precipitation in a High-Aqueous Phase Content Reverse Microemulsion
María G. Pineda (2014)
Efficient microwave synthesis, functionalisation and biocompatibility studies of SPION based potential nano-drug carriers
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