← Back to Search
PLGA Nanoparticles Stabilized With Cationic Surfactant: Safety Studies And Application In Oral Delivery Of Paclitaxel To Treat Chemical-Induced Breast Cancer In Rat
V. Bhardwaj, D. Ankola, S. Gupta, M. Schneider, C.-M. Lehr, M. Kumar
Published 2009 · Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
PurposeThis study was carried out to formulate poly(lactide-co-glycolide) (PLGA) nanoparticles using a quaternary ammonium salt didodecyl dimethylammonium bromide (DMAB) and checking their utility to deliver paclitaxel by oral route.MethodsParticles were prepared by emulsion solvent diffusion evaporation method. DMAB and particles stabilized with it were evaluated by MTT and LDH cytotoxicity assays. Paclitaxel was encapsulated in these nanoparticles and evaluated in a chemical carcinogenesis model in Sprague Dawley rats.ResultsMTT and LDH assays showed the surfactant to be safe to in vitro cell cultures at concentrations <33 μM. PLGA nanoparticles prepared using this stabilizer were also found to be non-toxic to cell lines for the duration of the study. When administered orally to rats bearing chemically induced breast cancer, nanoparticles were equally effective/better than intravenous paclitaxel in cremophor EL at 50% lower dose.ConclusionsThis study proves the safety and utility of DMAB in stabilizing preformed polymers like PLGA resulting in nanoparticles. This preliminary data provides a proof of concept of enabling oral chemotherapy by efficacy enhancement for paclitaxel.
This paper references
The Oral Absorption of Micro- and Nanoparticulates: Neither Exceptional Nor Unusual
A. Florence (2004)
Chemically induced mammary gland adenomyoepitheliomas and myoepithelial carcinomas of mice. Immunohistochemical and ultrastructural features.
S. Rehm (1990)
Nanomedicines for overcoming biological barriers.
M. Alonso (2004)
A phosphoglycoprotein associated with taxol resistance in J774.2 cells.
S. Roy (1985)
Development of potent oral nanoparticulate formulation of coenzyme Q10 for treatment of hypertension: can the simple nutritional supplements be used as first line therapeutic agents for prophylaxis/therapy?
D. Ankola (2007)
Biologically erodable microspheres as potential oral drug delivery systems
E. Mathiowitz (1997)
Nanomedicine gets clinical
R. Duncan (2005)
Polymer conjugates for tumour targeting and intracytoplasmic delivery. The EPR effect as a common gateway?
Pharmaceutical Aspects of Polymeric Nanoparticles for Oral Drug Delivery
V. Bhardwaj (2005)
Mammary Cancer Induced by a Single Feeding of Polynuclear Hydrocarbons, and its Suppression
C. Huggins (1961)
Estradiol loaded PLGA nanoparticles for oral administration: effect of polymer molecular weight and copolymer composition on release behavior in vitro and in vivo.
G. Mittal (2007)
Preparation and characterization of cationic PLGA nanospheres as DNA carriers.
M. Ravi Kumar (2004)
Targeted delivery of nanoparticles for the treatment of lung diseases.
S. Azarmi (2008)
Drug delivery: an odyssey of 100 years.
O. Pillai (2001)
Microtubule active taxanes inhibit polycystic kidney disease progression in cpk mice.
D. Woo (1997)
Neurotrophic Factors Stabilize Microtubules and Protect against Rotenone Toxicity on Dopaminergic Neurons*
Q. Jiang (2006)
Micellar paclitaxel improves severe psoriasis in a prospective phase II pilot study.
Alison Ehrlich (2004)
Effect of molecular structure of cationic surfactants on biophysical interactions of surfactant-modified nanoparticles with a model membrane and cellular uptake.
Chiranjeevi Peetla (2009)
Inhibition of experimental allergic encephalomyelitis in the Lewis rat by paclitaxel
Ligong Cao (2000)
Suspected anaphylactic reaction to Cremophor EL.
D. Dye (1980)
Formation of benzylic-DNA adducts resulting from 7,12-dimethylbenz[a]anthracene in vivo.
M. N. R. Ravi Kumar (2005)
Tumor production in rats injected intravenously with oil emulsions containing 9,10-dimethyl-1,2-benzanthracene.
R. Geyer (1951)
Controlled vaccine release in the gut-associated lymphoid tissues. I. Orally administered biodegradable microspheres target the peyer's patches
J. Eldridge (1990)
Regression of collagen-induced arthritis with taxol, a microtubule stabilizer.
E. Brahn (1994)
PLGA nanoparticles for oral delivery of hydrophobic drugs: influence of organic solvent on nanoparticle formation and release behavior in vitro and in vivo using estradiol as a model drug.
D. Sahana (2008)
The Mechanism of Uptake of Biodegradable Microparticles in Caco-2 Cells Is Size Dependent
M. P. Desai (2004)
Arterial uptake of biodegradable nanoparticles for intravascular local drug delivery: results with an acute dog model.
C. Song (1998)
Paclitaxel zur Alzheimer‐Behandlung
U. Holzgrabe (2005)
Nanocapsules: A new type of lysosomotropic carrier
P. Couvreur (1977)
S. Moghimi (2006)
Shape Induced Inhibition of Phagocytosis of Polymer Particles
J. Champion (2008)
Effect of glutamine on the initiation and promotion phases of DMBA-induced mammary tumor development.
Y. Kaufmann (2003)
The tumor blood vessel as an ideal target for macromolecular anticancer agents
H. Maeda (1992)
Tissue Distribution of [actinomycin-h-3 D Adsorbed On Polybutylcyanoacrylate Nanoparticles
B. Kante (1980)
Paclitaxel-loaded poly(L-lactic acid) microspheres 3: blending low and high molecular weight polymers to control morphology and drug release.
R. Liggins (2004)
Role of target geometry in phagocytosis.
J. Champion (2006)
Design of Estradiol Loaded PLGA Nanoparticulate Formulations: A Potential Oral Delivery System for Hormone Therapy
S. Hariharan (2005)
[Paclitaxel for Alzheimer treatment].
U. Holzgrabe (2005)
New polymeric nanomedicines for targeted and controlled drug delivery
J. Hanes (2006)
Design of biodegradable nanoparticles: a novel approach to encapsulating poorly soluble phytochemical ellagic acid
I. Bala (2005)
Design of nanoparticles composed of graft copolymers for oral peptide delivery.
S. Sakuma (2001)
This paper is referenced by
Improvement in antihypertensive and antianginal effects of felodipine by enhanced absorption from PLGA nanoparticles optimized by factorial design.
U. Shah (2014)
Paclitaxel/methotrexate co-loaded PLGA nanoparticles in glioblastoma treatment: Formulation development and in vitro antitumor activity evaluation.
F. Madani (2020)
Silk Fibroin and Hydroxyapatite Composites for Bioresorbable Bone Fixation Devices
Bryant Heimbach (2019)
The effect of the oral administration of polymeric nanoparticles on the efficacy and toxicity of tamoxifen.
A. Jain (2011)
Nanocarriers for Delivery of Herbal Based Drugs in Breast Cancer - An Overview
Kirtee Wani (2015)
Squalenoyl prodrug of paclitaxel: synthesis and evaluation of its incorporation in phospholipid bilayers.
M. G. Sarpietro (2012)
Formulations of Nanoparticles in Drug Delivery
Sushama Talegaonkar (2013)
Dexamethasone loaded PLGA nanoparticles for potential local treatment of oral precancerous lesions
S. Rençber (2019)
Various Challenges and Opportunities in Oral Delivery of Anticancer Drugs
N. Kumari (2019)
Cytotoxic and genotoxic characterization of titanium dioxide, gadolinium oxide, and poly(lactic-co-glycolic acid) nanoparticles in human fibroblasts.
M. I. Setyawati (2013)
Experimental Models as Refined Translational Tools for Breast Cancer Research
E. Costa (2020)
Gene delivery using dimethyldidodecylammonium bromide-coated PLGA nanoparticles.
F. Fay (2010)
Preparation, characterization, cellular uptake and evaluation in vivo of solid lipid nanoparticles loaded with cucurbitacin B
H. Hu (2013)
Effect of hydroxyapatite concentration on high-modulus composite for biodegradable bone-fixation devices.
Bryant Heimbach (2017)
CD44 targeted chemotherapy for co-eradication of breast cancer stem cells and cancer cells using polymeric nanoparticles of salinomycin and paclitaxel.
Eameema Muntimadugu (2016)
Microemulsions for oral delivery of insulin: design, development and evaluation in streptozotocin induced diabetic rats.
G. Sharma (2010)
Enhanced oral absorption of paclitaxel in N-deoxycholic acid-N, O-hydroxyethyl chitosan micellar system.
H. Li (2010)
Formulation and antitumorigenic activities of nanoencapsulated nifetepimine: A promising approach in treating triple negative breast carcinoma.
A. Ghosh (2016)
Improvement of oral efficacy of Irinotecan through biodegradable polymeric nanoparticles through in vitro and in vivo investigations
N. Ahmad (2018)
Fabrication of Compound K-loaded Polymeric Micelle System and its Characterization in vitro and Oral Absorption Enhancement in vivo
Sun-mi Hong (2014)
Oral delivery of anticancer drugs: challenges and opportunities.
K. Thanki (2013)
Mammalian models of chemically induced primary malignancies exploitable for imaging-based preclinical theragnostic research.
Y. Liu (2015)
Heteromer Nanostars by Spontaneous Self-Assembly
C. Brocker (2017)
Preferential cellular uptake of amphiphilic macromolecule-lipid complexes with enhanced stability and biocompatibility.
A. Harmon (2011)
Encapsulation of desmopressin into hydrophobic nanoparticles and hydrophilic microparticles for pulmonary drug delivery
Daniel Primavessy (2017)
Chitosan‐modified d‐α‐tocopheryl poly(ethylene glycol) 1000 succinate‐b‐poly(ε‐caprolactone‐ran‐glycolide) nanoparticles for the oral chemotherapy of bladder cancer
Baichuan Liu (2013)
Effect of Formulation Variables on Preparation of Celecoxib Loaded Polylactide-Co-Glycolide Nanoparticles
D. L. Cooper (2014)
Engineering of a novel adjuvant based on lipid-polymer hybrid nanoparticles: A quality-by-design approach.
Fabrice Rose (2015)
An Investigation into Formulation and Therapeutic Effectiveness of Nanoparticle Drug Delivery for Select Pharmaceutical Agents
D. L. Cooper (2016)
Formulation development, stability and anticancer efficacy of core-shell cyclodextrin nanocapsules for oral chemotherapy with camptothecin
H. Ünal (2015)
A microreactor-based continuous process for controlled synthesis of poly-methyl-methacrylate-methacrylic acid (PMMA) nanoparticles.
Anurag Dobhal (2017)
Stability of various PLGA and lipid nanoparticles in temperature and in time and new technology for the preparation of liposomes for anticancer and antibiotic loading
T. Mdzinarashvili (2019)See more