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Cytotoxicity And Oxidative Stress Responses Of Silica-coated Iron Oxide Nanoparticles In CHSE-214 Cells

K. Srikanth, T. Trindade, A. Duarte, E. Pereira
Published 2016 · Chemistry, Medicine

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The present study aimed at investigating cytotoxicity and oxidative stress induced by silica-coated iron oxide nanoparticles functionalized with dithiocarbamate (Fe3O4 NPs) in Chinook salmon cells (CHSE-214) derived from Oncorhynchus tshawytscha embryos. A significant reduction in cell viability was evident in response to Fe3O4 NPs as revealed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay after 24 h of exposure. Out of the tested concentrations (10, 20, and 30 μg/ml), the highest concentration has shown significant decrease in the viability of cells after 24 h of exposure. Alterations in the morphology of CHSE-214 cells was also evident at 10 μg/ml concentration of Fe3O4 NPs after 24 h. Fe3O4 NPs elicited a significant dose-dependent reduction in total glutathione content (TGSH), catalase (CAT), glutathione reductase (GR) with a concomitant increase in lipid peroxidation (LPO), and protein carbonyl (PC) at highest concentration (30 μg/ml) after 24 h of exposure. In conclusion, our data demonstrated that Fe3O4 NPs have potential to induce cytotoxicity in CHSE-214 cells, which is likely to be mediated through reactive oxygen species generation and oxidative stress.
This paper references
10.3109/17435390.2013.847505
Coating-dependent induction of cytotoxicity and genotoxicity of iron oxide nanoparticles
Z. Magdolénová (2015)
10.1186/1556-276X-9-304
Magnetic resonance imaging of glioma with novel APTS-coated superparamagnetic iron oxide nanoparticles
Kangan Li (2014)
10.2217/nnm.12.81
Interview: An architectural journey: from trees, dendrons/dendrimers to nanomedicine. Interview by Hannah Stanwix.
D. Tomalia (2012)
10.2174/1381612811319370011
Iron oxide nanoparticle-induced oxidative stress and genotoxicity in human skin epithelial and lung epithelial cell lines.
M. Ahamed (2013)
10.3402/nano.v1i0.5358
Potential toxicity of superparamagnetic iron oxide nanoparticles (SPION)
N. Singh (2010)
10.1016/0003-2697(90)90208-Q
Microtiter plate assay for the measurement of glutathione and glutathione disulfide in large numbers of biological samples.
M. Baker (1990)
10.1007/s12011-014-9972-0
Iron Oxide Nanoparticles Induce Oxidative Stress, DNA Damage, and Caspase Activation in the Human Breast Cancer Cell Line
S. Alarifi (2014)
10.1007/s00709-015-0849-7
Evaluation of cytotoxicity, morphological alterations and oxidative stress in Chinook salmon cells exposed to copper oxide nanoparticles
K. Srikanth (2015)
10.1016/S1873-0140(05)80005-0
Chapter 2 Use of fish cell lines in the toxicology and ecotoxicology of fish. Piscine cell lines in environmental toxicology
N. Bols (2005)
10.2331/FISHSCI.64.991
Application of a Suspension-Cultured Salmonid Cell Line CHSE-sp to Cytotoxicity Test
M. Mori (1998)
10.1371/journal.pone.0085835
Toxicity Assessment of Silica Coated Iron Oxide Nanoparticles and Biocompatibility Improvement by Surface Engineering
M. A. Malvindi (2014)
10.1007/s11356-015-4125-3
Lipid peroxidation and its control in Anguilla anguilla hepatocytes under silica-coated iron oxide nanoparticles (with or without mercury) exposure
K. Srikanth (2015)
10.1007/s12013-012-9367-9
Oxidative Stress and Dermal Toxicity of Iron Oxide Nanoparticles In Vitro
A. Murray (2012)
10.1016/j.biomaterials.2010.08.075
Cytotoxic effects of iron oxide nanoparticles and implications for safety in cell labelling.
Stefaan J.H. Soenen (2011)
10.1016/0022-1759(83)90303-4
Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays.
T. Mosmann (1983)
10.1016/0003-2697(69)90064-5
Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione: applications to mammalian blood and other tissues.
F. Tietze (1969)
10.1016/j.tiv.2008.07.006
Toxic effects of DDT and methyl mercury on the hepatocytes from Hoplias malabaricus.
F. Filipak Neto (2008)
[Use of fish cell cultures for toxicity determination in order to reduce and replace the fish tests].
W. Ahne (1985)
Purification and characterization of the flavoenzyme glutathione reductase from rat liver.
I. Carlberg (1975)
10.1021/JP904884Y
Multiphysics Flow Modeling and in Vitro Toxicity of Iron Oxide Nanoparticles Coated with Poly(vinyl alcohol)
M. Mahmoudi (2009)
10.1016/j.antiviral.2008.07.007
Inhibitory effect of mycophenolic acid on the replication of infectious pancreatic necrosis virus and viral hemorrhagic septicemia virus.
L. Marroqui (2008)
10.1016/j.colsurfb.2014.06.064
Synthesis, characterization and toxicological evaluation of iron oxide nanoparticles in human lung alveolar epithelial cells.
S. Dwivedi (2014)
10.1021/nn800596w
Cytotoxicity and genotoxicity of silver nanoparticles in human cells.
P. V. AshaRani (2009)
10.1371/journal.pone.0046286
Toxicity Assessment of Iron Oxide Nanoparticles in Zebrafish (Danio rerio) Early Life Stages
Xiaoshan Zhu (2012)
10.1016/J.HAL.2010.08.007
Toxicity of the ichthyotoxic marine flagellate Pseudochattonella (Dictyochophyceae, Heterokonta) assessed by six bioassays
B. Skjelbred (2011)
10.7326/0003-4819-83-6-919_1
Red Cell Metabolism: A Manual of Biochemical Methods
E. Beutler (1975)
10.1002/NBM.970
A model of lysosomal metabolism of dextran coated superparamagnetic iron oxide (SPIO) nanoparticles: implications for cellular magnetic resonance imaging
A. Arbab (2005)
10.3109/02713683.2013.845228
ARPE-19 Cell Uptake of Small and Ultrasmall Superparamagnetic Iron Oxide
G. Grottone (2014)
10.1016/S0076-6879(84)05038-2
Comparative studies on different methods of malonaldehyde determination.
R. Bird (1984)
Glutathione S-transferases. The first enzymatic step in mercapturic acid formation.
W. Habig (1974)
Catalase activity
RA Greenwald (1985)
10.1016/J.CEJ.2014.06.008
The role of operational parameters on the uptake of mercury by dithiocarbamate functionalized particles
D. Tavares (2014)
10.1002/jat.3142
Aluminium oxide nanoparticles induced morphological changes, cytotoxicity and oxidative stress in Chinook salmon (CHSE‐214) cells
K. Srikanth (2015)
10.1088/0957-4484/21/39/395103
Degradability of superparamagnetic nanoparticles in a model of intracellular environment: follow-up of magnetic, structural and chemical properties.
M. Levy (2010)
10.1016/J.ECOENV.2004.06.009
In vitro and in vivo comparisons of fish-specific CYP1A induction relative potency factors for selected polycyclic aromatic hydrocarbons.
S. M. Billiard (2004)
10.1016/0003-2697(76)90527-3
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
M. M. Bradford (1976)
10.1016/0043-1354(68)90060-2
Fish cells in culture for study of aquatic toxicants
J. W. Rachlin (1968)
Flow Modeling and in Vitro Toxicity of Iron Oxide Nanoparticles Coated with Poly ( vinyl alcohol )
Morteza Mahmoudi (2009)
10.1016/0076-6879(90)86141-H
Determination of carbonyl content in oxidatively modified proteins.
R. L. Levine (1990)
Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction
大川 博 (1979)
10.1016/J.TOX.2006.04.042
Proposal to improve vertebrate cell cultures to establish them as substitutes for the regulatory testing of chemicals and effluents using fish.
K. Schirmer (2006)
10.4236/JBISE.2015.84026
Iron Oxide Nanoparticles Induced Oxidative Damage in Peripheral Blood Cells of Rat
Usha Singh Gaharwar (2015)
10.18388/ABP.2010_2416
Depletion of intracellular glutathione and increased lipid peroxidation mediate cytotoxicity of hematite nanoparticles in MRC-5 cells.
M. Radu (2010)
10.1016/J.JKSUS.2014.11.002
Iron oxide nanoparticles to an Indian major carp, Labeo rohita: Impacts on hematology, iono regulation and gill Na+/K+ ATPase activity
Anand Sadanandan Remya (2015)
10.1016/j.cbpc.2014.01.007
In vitro assay for the toxicity of silver nanoparticles using heart and gill cell lines of Catla catla and gill cell line of Labeo rohita.
G. Taju (2014)
10.3109/17435390.2013.790997
Species-specific toxicity of copper nanoparticles among mammalian and piscine cell lines
Lan Song (2014)
10.3109/17435390.2013.855830
Toxicity evaluation of engineered nanoparticles for medical applications using pulmonary epithelial cells
R. Guadagnini (2015)
10.2147/IJN.S28316
Enhanced cellular uptake of aminosilane-coated superparamagnetic iron oxide nanoparticles in mammalian cell lines
X. Zhu (2012)
10.1111/j.1582-4934.2006.tb00407.x
Protein carbonylation, cellular dysfunction, and disease progression
I. Dalle-donne (2006)
10.1002/ADEM.200990035
Cytotoxicity and Cell Cycle Effects of Bare and Poly(vinyl alcohol)‐Coated Iron Oxide Nanoparticles in Mouse Fibroblasts
M. Mahmoudi (2009)
10.1046/j.1471-4159.1998.70010268.x
Increased Protein Oxidation in Human Substantia Nigra Pars Compacta in Comparison with Basal Ganglia and Prefrontal Cortex Measured with an Improved Dinitrophenylhydrazine Assay
E. Floor (1998)
10.1007/s11356-012-1459-y
Glutathione and its dependent enzymes’ modulatory responses to toxic metals and metalloids in fish—a review
K. Srikanth (2013)



This paper is referenced by
10.3390/ijerph15010027
Association between Exposure to Ambient Air Particulates and Metabolic Syndrome Components in a Saudi Arabian Population
M. Shamy (2017)
10.1002/JLB.5MR0420-008RR
Magnetic nanoparticles: A new diagnostic and treatment platform for rheumatoid arthritis.
Y. Liu (2020)
10.1159/000485020
Physicochemical Properties of Magnetic Nanoparticles: Implications for Biomedical Applications In Vitro and In Vivo
A. Belanova (2018)
10.2147/IJN.S129375
Involvement of PINK1/parkin-mediated mitophagy in ZnO nanoparticle-induced toxicity in BV-2 cells
Limin Wei (2017)
10.1007/978-981-15-1702-0_13
Routes of Exposures and Toxicity of Nanoparticles
K. Srikanth (2020)
10.1007/978-981-15-1702-0
Model Organisms to Study Biological Activities and Toxicity of Nanoparticles
Busi Siddhardha (2020)
Engineering of polymeric nanoparticles based on structure-activity relationships (SARs) for oral drug delivery
U. Odunze (2018)
10.1007/978-3-319-72041-8_6
Nanoparticles-Caused Oxidative Imbalance.
M. Żuberek (2018)
10.1016/j.nano.2018.01.014
Immunological effects of iron oxide nanoparticles and iron-based complex drug formulations: Therapeutic benefits, toxicity, mechanistic insights, and translational considerations.
Ankit Shah (2018)
10.1080/01480545.2017.1384005
Effects of silica nanoparticles on isolated rat uterine smooth muscle
S. Yaman (2018)
10.3390/toxics6040062
The Toxicity Assessment of Iron Oxide (Fe3O4) Nanoparticles on Physical and Biochemical Quality of Rainbow Trout Spermatozoon
M. Özgür (2018)
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