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Engineering Escherichia Coli To Sense Acidic Amino Acids By Introduction Of A Chimeric Two-component System

S. Ravikumar, Irisappan Ganesh, M. Maruthamuthu, S. Hong
Published 2015 · Chemistry

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In an attempt to create an acidic amino acid-sensing Escherichia coli, a chimeric sensor kinase (SK)-based biosensor was constructed using Pseudomonas putida AauS. AauS is a sensor kinase that ultimately controls expression of the aau gene through its cognate response regulator AauR, and is found only in P. putida KT2440. The AauZ chimera SK was constructed by integration of the sensing domain of AauS with the catalytic domain of EnvZ to control the expression of the ompC gene in response to acidic amino acids. Real-time quantitative PCR and GFP fluorescence studies showed increased ompC gene expression and GFP fluorescence as the concentration of acidic amino acids increased. These data suggest that AauS-based recombinant E. coli can be used as a bacterial biosensor of acidic amino acids. By employing the chimeric SK strategy, various bacteria biosensors for use in the development of biochemical-producing recombinant microorganisms can be constructed.
This paper references
Activation of bacterial porin gene expression by a chimeric signal transducer in response to aspartate.
R. Utsumi (1989)
Computational methods for the analysis of chemical sensor array data from volatile analytes.
P. Jurs (2000)
Histidine kinases and response regulator proteins in two-component signaling systems.
A. West (2001)
Microbial production of l-amino-acids
R. Faurie (2003)
Industrial production of amino acids by coryneform bacteria.
T. Hermann (2003)
Comparison of two standardisation methods in real-time quantitative RT-PCR to follow Staphylococcus aureus genes expression during in vitro growth.
H. Eleaume (2004)
Synthetic biology: Engineering Escherichia coli to see light
Anselm Levskaya (2005)
Real-time risk monitoring system for chemical plants
K. Kim (2005)
The AauR-AauS Two-Component System Regulates Uptake and Metabolism of Acidic Amino Acids in Pseudomonas putida
A. Sonawane (2006)
Evolution of prokaryotic two-component system signaling pathways: gene fusions and fissions.
P. Cock (2007)
Food additives and sensitivities.
C. Rangan (2009)
Importance of expression system in the production of unnatural recombinant proteins in Escherichia coli
N. Ayyadurai (2009)
MzrA-EnvZ Interactions in the Periplasm Influence the EnvZ/OmpR Two-Component Regulon
Henri G. Gerken (2010)
Expression characteristics of the maeA and maeB genes by extracellular malate and pyruvate in Escherichia coli
I. Ganesh (2013)
Quantitative analysis of the three main genera in effective microorganisms using qPCR
Kihun Ahn (2014)

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