Online citations, reference lists, and bibliographies.
← Back to Search

Engineering Escherichia Coli To Sense Acidic Amino Acids By Introduction Of A Chimeric Two-component System

S. Ravikumar, Irisappan Ganesh, M. Maruthamuthu, S. Hong
Published 2015 · Chemistry

Save to my Library
Download PDF
Analyze on Scholarcy Visualize in Litmaps
Share
Reduce the time it takes to create your bibliography by a factor of 10 by using the world’s favourite reference manager
Time to take this seriously.
Get Citationsy
In an attempt to create an acidic amino acid-sensing Escherichia coli, a chimeric sensor kinase (SK)-based biosensor was constructed using Pseudomonas putida AauS. AauS is a sensor kinase that ultimately controls expression of the aau gene through its cognate response regulator AauR, and is found only in P. putida KT2440. The AauZ chimera SK was constructed by integration of the sensing domain of AauS with the catalytic domain of EnvZ to control the expression of the ompC gene in response to acidic amino acids. Real-time quantitative PCR and GFP fluorescence studies showed increased ompC gene expression and GFP fluorescence as the concentration of acidic amino acids increased. These data suggest that AauS-based recombinant E. coli can be used as a bacterial biosensor of acidic amino acids. By employing the chimeric SK strategy, various bacteria biosensors for use in the development of biochemical-producing recombinant microorganisms can be constructed.
This paper references
10.1126/SCIENCE.2476847
Activation of bacterial porin gene expression by a chimeric signal transducer in response to aspartate.
R. Utsumi (1989)
10.1021/CR9800964
Computational methods for the analysis of chemical sensor array data from volatile analytes.
P. Jurs (2000)
10.1016/S0968-0004(01)01852-7
Histidine kinases and response regulator proteins in two-component signaling systems.
A. West (2001)
10.1007/3-540-45989-8
Microbial production of l-amino-acids
R. Faurie (2003)
10.1016/S0168-1656(03)00149-4
Industrial production of amino acids by coryneform bacteria.
T. Hermann (2003)
10.1016/J.MIMET.2004.07.015
Comparison of two standardisation methods in real-time quantitative RT-PCR to follow Staphylococcus aureus genes expression during in vitro growth.
H. Eleaume (2004)
10.1038/nature04405
Synthetic biology: Engineering Escherichia coli to see light
Anselm Levskaya (2005)
10.1007/BF02701457
Real-time risk monitoring system for chemical plants
K. Kim (2005)
10.1128/AEM.00830-06
The AauR-AauS Two-Component System Regulates Uptake and Metabolism of Acidic Amino Acids in Pseudomonas putida
A. Sonawane (2006)
10.1093/MOLBEV/MSM170
Evolution of prokaryotic two-component system signaling pathways: gene fusions and fissions.
P. Cock (2007)
10.1016/j.disamonth.2009.01.004
Food additives and sensitivities.
C. Rangan (2009)
10.1007/S12257-009-0009-Z
Importance of expression system in the production of unnatural recombinant proteins in Escherichia coli
N. Ayyadurai (2009)
10.1128/JB.00855-10
MzrA-EnvZ Interactions in the Periplasm Influence the EnvZ/OmpR Two-Component Regulon
Henri G. Gerken (2010)
10.1007/s11814-013-0061-4
Expression characteristics of the maeA and maeB genes by extracellular malate and pyruvate in Escherichia coli
I. Ganesh (2013)
10.1007/s11814-013-0274-6
Quantitative analysis of the three main genera in effective microorganisms using qPCR
Kihun Ahn (2014)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar