Online citations, reference lists, and bibliographies.

The Role Of PCR In The Diagnosis Of Candida Vulvovaginitis—a New Gold Standard?

J. Sobel, R. Akins
Published 2015 · Medicine

Cite This
Download PDF
Analyze on Scholarcy
Share
PCR is recognized as a reliable technique for detection of all types of microorganisms. Being highly objective and reproducible also sensitive and specific, PCR is now widely used for sexually transmitted infection (STI) diagnosis. Potential, however, exists for detecting non-pathogens, and not identifying a pathogenic state decreases specificity or clinical significance. PCR Candida tests of vaginal specimens are now widely available and frequently used offering a modest to moderate increase in sensitivity and are likely to replace traditional culture and DNA homology testing. Nevertheless, there remain considerable gaps in our knowledge regarding the usefulness and applications of these expensive tests.
This paper references
10.1016/J.DIAGMICROBIO.2007.09.014
Detection and identification of Candida spp. in human serum by LightCycler real-time polymerase chain reaction.
C. Dunyach (2008)
10.1111/J.1469-0691.2006.01498.X
Molecular detection and identification of Candida and Aspergillus spp. from clinical samples using real-time PCR.
L. Klingspor (2006)
10.1097/AOG.0b013e3181909f63
Accuracy of the Clinical Diagnosis of Vaginitis Compared With a DNA Probe Laboratory Standard
N. Lowe (2009)
10.1136/jcp.50.7.563
Use of CHROMagar Candida for genital specimens in the diagnostic laboratory.
E. Houang (1997)
10.1128/JCM.02485-07
Survey of Vaginal-Flora Candida Species Isolates from Women of Different Age Groups by Use of Species-Specific PCR Detection
John-Paul Vermitsky (2008)
10.1128/jcm.32.8.1923-1929.1994
CHROMagar Candida, a new differential isolation medium for presumptive identification of clinically important Candida species.
F. Odds (1994)
[Distribution of Candida species in vaginal specimens and evaluation of CHROMagar Candida medium].
Berna Gültekin (2005)
10.2353/jmoldx.2010.090085
High-throughput identification and quantification of Candida species using high resolution derivative melt analysis of panfungal amplicons.
Tasneem Mandviwala (2010)
10.1128/JCM.01537-13
Comparison of Nucleic Acid Amplification Assays with BD Affirm VPIII for Diagnosis of Vaginitis in Symptomatic Women
C. Cartwright (2013)
10.1128/JCM.36.11.3260-3265.1998
Rapid Identification of Candida Species with Species-Specific DNA Probes
C. Elie (1998)
[Vulvovaginal candidiasis: prevalence of different Candida species in the Liege region].
J. Senterre (2005)
10.1128/JCM.38.8.3016-3021.2000
Simple and Rapid Detection of Candida albicans DNA in Serum by PCR for Diagnosis of Invasive Candidiasis
R. Wahyuningsih (2000)
10.1016/J.MCP.2004.10.004
Detection and identification of Candida species associated with Candida vaginitis by real-time PCR and pyrosequencing.
J. Trama (2005)
10.1128/JCM.38.10.3735-3742.2000
Development of a PCR-Based Line Probe Assay for Identification of Fungal Pathogens
C. Martín (2000)
10.1007/978-1-62703-257-5_9
Species-specific identification of a wide range of clinically relevant fungal pathogens by the Luminex(®) xMAP technology.
Sandra Preuner (2013)
10.1099/JMM.0.47212-0
The Pan-AC assay: a single-reaction real-time PCR test for quantitative detection of a broad range of Aspergillus and Candida species.
L. Bašková (2007)
10.1099/00222615-39-3-233
Application of the polymerase chain reaction to the diagnosis of candidosis by amplification of an HSP 90 gene fragment.
A. Crampin (1993)
10.1155/2013/281892
Codetection of Trichomonas vaginalis and Candida albicans by PCR in Urine Samples in a Low-Risk Population Attended in a Clinic First Level in Central Veracruz, Mexico
Aracely López-Monteon (2013)
10.1128/JCM.01558-08
Species-Specific Identification of a Wide Range of Clinically Relevant Fungal Pathogens by Use of Luminex xMAP Technology
C. Landlinger (2009)
10.1016/J.MIMET.2007.01.011
Rapid simultaneous detection and identification of six species Candida using polymerase chain reaction and reverse line hybridization assay.
H. Xiang (2007)
10.1128/JCM.00028-13
Evaluation of PNA-FISH Yeast Traffic Light for Rapid Identification of Yeast Directly from Positive Blood Cultures and Assessment of Clinical Impact
N. Stone (2013)
10.1155/2005/401530
Vaginal Candida parapsilosis: pathogen or bystander?
P. Nyirjesy (2005)
10.1016/j.diagmicrobio.2010.10.003
Rapid, simple, and low-cost identification of Candida species using high-resolution melting analysis.
S. Arancia (2011)
10.1111/MYC.12204
Vulvovaginal candidiasis: species distribution, fluconazole resistance and drug efflux pump gene overexpression.
Jie-yu Zhang (2014)
10.3109/13693780802713224
Performance of Chromogenic Candida agar and CHROMagar Candida in recovery and presumptive identification of monofungal and polyfungal vaginal isolates.
K. Ozcan (2010)
10.1128/.38.6.2302-2310.2000
Identification of medically important yeasts using PCR-based detection of DNA sequence polymorphisms in the internal transcribed spacer 2 region of the rRNA genes.
Y. Chen (2000)
10.1016/j.mycmed.2014.01.060
Species distribution and susceptibility of Candida isolates from patient with vulvovaginal candidiasis in Southern China from 2003 to 2012.
X. Liu (2014)
10.1128/JCM.41.7.3293-3298.2003
Rapid Detection of Candida albicans in Clinical Blood Samples by Using a TaqMan-Based PCR Assay
Y. Maaroufi (2003)
Prevalence des differentes especes de Candida au niveau vaginal dans la region Liegeoise
J. Senterre (2005)
10.1111/myc.12205
Comparative analysis of Gram's stain, PNA-FISH and Sepsityper with MALDI-TOF MS for the identification of yeast direct from positive blood cultures.
Rebecca Louise Gorton (2014)
10.1128/jcm.35.6.1454-1459.1997
Rapid identification of Candida species in blood cultures by a clinically useful PCR method.
J. Shin (1997)
10.1128/JCM.40.8.2860-2865.2002
Rapid Identification of Pathogenic Fungi Directly from Cultures by Using Multiplex PCR
G. Luo (2002)
10.1258/09564620360719796
Detection of candida by polymerase chain reaction vs microscopy and culture in women diagnosed as recurrent vulvovaginal cases
P. Mårdh (2003)
10.1001/JAMA.291.11.1368
Evaluation of vaginal complaints.
M. Anderson (2004)
10.1186/1476-0711-5-4
Throwing the dice for the diagnosis of vaginal complaints?
A. Schwiertz (2005)
10.1128/JCM.38.4.1609-1614.2000
Reverse Cross Blot Hybridization Assay for Rapid Detection of PCR-Amplified DNA from Candida Species, Cryptococcus neoformans, and Saccharomyces cerevisiae in Clinical Samples
B. Posteraro (2000)
10.3109/13693786.2010.497972
Evaluation of risk factors in patients with vulvovaginal candidiasis and the value of chromID Candida agar versus CHROMagar Candida for recovery and presumptive identification of vaginal yeast species.
Ahmet Barış Guzel (2011)
Real-time LightCycler polymerase chain reaction and melting temperature analysis for identification of clinically important Candida spp.
Ziauddin Khan (2009)
Species distribution and susceptibility of Candida isolates from patient with vulvovaginal candidiasis in Southern China
XP Liu (2003)
10.1007/s00404-008-0681-9
Relationship between clinical diagnosis of recurrent vulvovaginal candidiasis and detection of Candida species by culture and polymerase chain reaction
T. Weissenbacher (2008)
10.1016/S0029-7844(99)00577-3
Vaginal Colonization by Candida in Asymptomatic Women With and Without a History of Recurrent Vulvovaginal Candidiasis
P. Giraldo (2000)
10.1128/jcm.32.12.2962-2967.1994
PCR identification of four medically important Candida species by using a single primer pair.
J. Jordan (1994)



This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar