← Back to Search
Isolation And Characterization Of Chromium(VI)-Reducing Bacteria From Tannery Effluents
M. Ilias, I. Rafiqullah, B. C. Debnath, K. S. B. Mannan, Md. Mozammel Hoq
Published 2011 · Biology, Medicine
Save to my Library
Download PDFAnalyze on Scholarcy
Two chromium-resistant bacteria (IFR-2 and IFR-3) capable of reducing/transforming Cr(VI) to Cr(III) were isolated from tannery effluents. Isolates IFR-2 and IFR-3 were identified as Staphylococcus aureus and Pediococcus pentosaceus respectively by 16S rRNA gene sequence analyses. Both isolates can grow well on 2,000 mg/l Cr(VI) (as K2Cr2O7) in Luria-Bertani (LB) medium. Reduction of Cr(VI) was found to be growth-associated in both isolates and IFR-2 and IFR-3 reduced 20 mg/l Cr(VI) completely in 6 and 24 h respectively. The Cr(VI) reduction due to chromate reductase activity was detected in the culture supernatant and cell lysate but not at all in the cell extract supernatant of both isolates. Whole cells of IFR-2 and IFR-3 converted 24 and 30% of the initial Cr(VI) concentration (1 mg/l) in 45 min respectively at 37°C. NiCl2 stimulated the growth of IFR-2 whereas HgCl2 and CdCl2 significantly inhibited the growth of both isolates. Optimum temperature and pH for growth of and Cr(VI) reduction by both isolates were found to be between 35 and 40°C and pH 7.0 to 8.0. The two bacterial isolates can be good candidates for detoxification of Cr(VI) in industrial effluents.
This paper references
Reduction of Chromate by Desulfovibrio vulgaris and Its c(3) Cytochrome.
D. Lovley (1994)
Molecular Cloning: A Laboratory Manual
J. Sambrook (1983)
Isolation and Characterization of an Enterobacter cloacae Strain That Reduces Hexavalent Chromium under Anaerobic Conditions.
P. C. Wang (1989)
Mechanisms of bacterial resistance to chromium compounds
M. I. Ramírez-Díaz (2007)
Characterization of enzymatic reduction of hexavalent chromium by Escherichia coli ATCC 33456.
H. Shen (1993)
Isolation and Characterization of Chromium-Resistant Bacteria from Tannery Effluents
M. Basu (1997)
Membrane-associated chromate reductase activity from Enterobacter cloacae.
P. C. Wang (1990)
Chromate resistance plasmid in Pseudomonas fluorescens.
L. Bopp (1983)
Reduction of Toxic Chromium and Partial Localization of Chromium Reductase Activity in Bacterial Isolate DM1
Urvashi Thacker (2005)
In vitro reduction of hexavalent chromium by a cell-free extract of Bacillus sp. ES 29 stimulated by Cu2+
F. Camargo (2003)
Chromate Reduction by a Pseudomonad Isolated from a Site Contaminated with Chromated Copper Arsenate
J. McLean (2001)
Chromate reduction by chromium-resistant bacteria isolated from soils contaminated with dichromate.
F. A. O. Camargo (2003)
Sorption of heavy metals to the filamentous bacterium thiothrix strain A1.
Kay L. Shuttleworth (1993)
NAD(P)H-dependent chromium (VI) reductase of Pseudomonas ambigua G-1: a Cr(V) intermediate is formed during the reduction of Cr(VI) to Cr(III).
T. Suzuki (1992)
Chromium(III) Hydrolysis Constants and Solubility of Chromium(III) Hydroxide.
D. Rai (1987)
Isolation and characterization of a chromium-reducing bacterium from a chromated copper arsenate-contaminated site.
J. McLean (2000)
Removal of toxic chromate using free and immobilized Cr(VI)-reducing bacterial cells of Intrasporangium sp. Q5-1
J. Yang (2009)
Distribution of bacterioplankton in meromictic Lake Saelenvannet, as determined by denaturing gradient gel electrophoresis of PCR-amplified gene fragments coding for 16S rRNA.
L. Ovreås (1997)
Basic local alignment search tool.
S. Altschul (1990)
Bioremediation of toxic chromium from electroplating effluent by chromate-reducing Pseudomonas aeruginosa A2Chr in two bioreactors
A. Ganguli (2001)
Chromium. In: Sparks DL (ed) Methods of soil analysis, Part 3, SSSA Book Series
RJ Bartlett (1996)
This paper is referenced by
Three-Stage Single-Chambered Microbial Fuel Cell Biosensor Inoculated with Exiguobacterium aestuarii YC211 for Continuous Chromium (VI) Measurement
Li-Chun Wu (2019)
Genotoxicity evaluation of tannery effluent treated with newly isolated hexavalent chromium reducing Bacillus cereus.
V. Kumari (2016)
Cr(VI) removal from aqueous solution by thermophilic denitrifying bacterium Chelatococcus daeguensis TAD1 in the presence of single and multiple heavy metals
H. Li (2016)
The Exploitation of Microbes: Next Generation Global Solution
M. A. Shishir (2020)
Isolation and identification of chromium reducing bacteria from tannery effluent
M. Sanjay (2020)
ISOLATION AND BIOCHEMICAL CHARACTERIZATION OF CHROME RESISTANT BACTERIA FROM SOIL SAMPLES OF MUSLIM BAGH MINES
Neelam Hashmi (2018)
Potential Microbial Diversity in Chromium Mining Areas: A Review
Alolika Basu (2015)
Antibiotic Resistant Genes in Natural Environment
Tejpreet Chadha (2012)
Optimization of Growth Medium and Biosorption of Chromium Using Micro Algae and Cyanobacteria
R. Kasimani (2017)
Reduction of Hexavalent Chromium and Detection of Chromate Reductase (ChrR) in Stenotrophomonas maltophilia
R. Baldiris (2018)
Microbially Assisted Phytoremediation of Heavy Metal–Contaminated Soils
D. Egamberdieva (2016)
Bacterial mechanisms for Cr(VI) resistance and reduction: an overview and recent advances
M. Ahemad (2014)
Bacteria With Heavy Metal Bioremediation Potential Isolated From The Polluted River Water Of Bangladesh
Sanjeeda Haider Nupur (2020)
Simultaneous chromate reduction and azo dye decolourization by Lactobacillus paracase CL1107 isolated from deep sea sediment.
Guangdao Huang (2015)
Microbial Biotransformation of Hexavalent Chromium [Cr(VI)] in Tannery Wastewater
A. Deepa (2020)
Simultaneous removal of malachite green and hexavalent chromium by Cunninghamella elegans biofilm in a semi-continuous system
S. Hussain (2017)
Use of RSM modeling for optimizing decolorization of simulated textile wastewater by Pseudomonas aeruginosa strain ZM130 capable of simultaneous removal of reactive dyes and hexavalent chromium
Zahid Maqbool (2016)
Dechlorination of chloroorganics, decolorization, and simultaneous bioremediation of Cr6+ from real tannery effluent employing indigenous Bacillus cereus isolate
Manikant Tripathi (2014)
Enzymatic Chromium (VI) Reduction by Cytoplasmic and Cell Membrane Fractions of Chromate-Reducing Bacterium Isolated From Sewage Treatment Plant
Paul Fabrice Nguema (2014)
Characterization of chromate reducing Pseudomonas aeruginosa strain Mie3 isolated from Juru River sludge and its potential on Azo dye decolorization
M. I. E. Halmi (2017)
BIOREMOVAL OF TOXIC CHROMIUM(VI) VIA DARK HYDROGEN FERMENTATION OF MULTICOMPONENT ORGANIC WASTE
V. .. Hovorukha (2020)
Biotransformation of chromium (VI) by Bacillus sp. isolated from chromate contaminated landfill site
M. E. Karim (2020)
The Role of Microbes in Chromium Bioremediation of Tannery Effluent
Pratishtha Gupta (2019)
Screening of plant growth promoting traits in heavy metals resistant bacteria: Prospects in phytoremediation
N. Tirry (2018)
Remobilization of Trivalent Chromium and the Regeneration of In Situ Permeable Reactive Barriers during Operation
L. A. Kaimbi (2013)
Synchronous detoxification and reduction treatment of tannery sludge using Cr (VI) resistant bacterial strains.
Haijin Liu (2019)
Chromium speciation, bioavailability, uptake, toxicity and detoxification in soil-plant system: A review.
Muhammad Shahid (2017)
Optimization of cultural conditions for growth associated chromate reduction by Arthrobacter sp. SUK 1201 isolated from chromite mine overburden.
S. Dey (2012)
Heavy Metal Tolerance and Antibiotic Resistance of Bacillus spp. Isolated from Two Major Rivers in Bangladesh
Tahmina Shammi (2016)
Capability of plant growth-promoting bacteria in chromium-contaminated soil after application of composted tannery sludge
S. Rocha (2019)
Chromium-Resistant Bacteria and Their Environmental Condition for Hexavalent Chromium Removal: A Review
M. Narayani (2013)
Metal-tolerant Pseudomonas aeruginosa Strain ZM130 has the Potential for Concurrent Dye Decolorization and Plant Growth Promotion
Zahid Maqbool (2018)See more