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Determination Of The Microbial Flora In Traditional İzmir Tulum Cheeses By Denaturing Gradient Gel Electrophoresis

Burçin Karabey, D. Eroğlu, C. Vural, G. Ozdemir, O. Yerlikaya, O. Kinik
Published 2018 · Biology, Medicine

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In this study, it was aimed to determine microbial flora members in three traditional Tulum cheeses (C1, C2 and C3) produced in different villages and settlement areas in İzmir, Turkey. For this purpose, culture depended and 16S rRNA based culture independent methods were used. According to the results of culture depended method, Lactococcus spp., Enterococcus spp., Staphylococcus spp., Lactobacillus spp., Pediococcus spp. and yeast-mold were detected in all samples at different levels. In order to determine and identify both of the culturable and non-culturable microorganisms, denaturing gradient gel electrophoresis (DGGE) method was used. DGGE results have shown that there were eight different dominant microorganisms (Streptococcus thermophilus, Lactococcus lactis subs. lactis, Streptococcus infantarius subs. infantarius, Lactobacillus gallinarum, Streptococcus equinus, Enterococcus faecalis, Enterococcus faecium, Lactococcus garvieae) in three regionally cheese samples. Further more, total bacterial loads were monitored with real-time PCR (qPCR) method. According to the results, 3.5 × 108, 3.8 × 108, 8.4 × 108 copy number of DNA was detected in C1, C2 and C3 cheese samples, respectively. This study is the first description for the dynamics of microbial composition of Izmir Tulum cheese after the production and brining processes.
This paper references
The Traditional Cheeses of Turkey: The Aegean Region
U. Kamber (2007)
Culture-independent methods for studying environmental microorganisms: methods, application, and perspective
Can Su (2011)
Influence of ripening container on the lactic acid bacteria population in Tulum cheese
S. Çakmakçi (2008)
Quantitative real-time PCR using TaqMan and SYBR Green for Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, tetQ gene and total bacteria.
H. Maeda (2003)
Diversity and dynamics of the microbial community during the manufacture of Calenzana, an artisanal Corsican cheese.
E. Casalta (2009)
Biodiversity of Bacterial Ecosystems in Traditional Egyptian Domiati Cheese
Gaber A. H. El-Baradei (2006)
PCR-DGGE as a tool for characterizing dominant microbial populations in the Spanish blue-veined Cabrales cheese
A. Flórez (2006)
Application of Denaturing Gradient Gel Electrophoresis (DGGE) To Study the Diversity of Marine Picoeukaryotic Assemblages and Comparison of DGGE with Other Molecular Techniques
B. Díez (2001)
The Traditional Cheeses of Turkey: Cheeses Common to All Regions
U. Kamber (2007)
Identification of Lactic Acid Bacteria Isolated from Tulum Cheese During Ripening Period
M. Gurses (2006)
The potential of a polyphasic PCR-dGGE approach in evaluating microbial diversity of natural whey cultures for water-buffalo Mozzarella cheese production: bias of culture-dependent and culture-independent analyses.
D. Ercolini (2001)
Diversity and evolution of the microbial populations during manufacture and ripening of Casín, a traditional Spanish, starter-free cheese made from cow's milk.
Á. Alegría (2009)
Bacterial chromosomal painting for in situ monitoring of cultured marine bacteria.
B. Lanoil (2000)
Design and Evaluation of PCR Primers for Analysis of Bacterial Populations in Wine by Denaturing Gradient Gel Electrophoresis
Isabel Cristina Buriticá López (2003)
Culture-independent methods for identifying microbial communities in cheese.
Jean-Luc Jany (2008)
Bacterial diversity of Darfiyeh, a Lebanese artisanal raw goat's milk cheese.
Mireille Serhan (2009)
Utilization and characterization of small ruminants' milk and milk products in Turkey: Current status and new perspectives
A. Hayaloğlu (2011)
The effect of quality properties on Tulum cheese using different package materials
Nazif Bayar (2011)
Molecular approaches to analysing the microbial composition of raw milk and raw milk cheese.
L. Quigley (2011)
DNA-based, culture-independent strategies for evaluating microbial communities in food-associated ecosystems.
G. Giraffa (2001)
An overview of researches on cheeses ripening in animal skin
M. T. Kalit (2010)
Cheeses of Turkey: 1. Varieties ripened in goat-skin bags
A. Hayaloğlu (2007)
Microbiological characterisation of Robiola di Roccaverano cheese using PCR-DGGE.
Silvia Bonetta (2008)
Application of denaturing gradient gel electrophoresis (DGGE) and temperature gradient gel electrophoresis (TGGE) in microbial ecology
G. Muyzer (2004)
Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA.
G. Muyzer (1993)
Determination of Microbiological, Physico-Chemical Properties and Distribution of Lactic Acid Bacteria of Afyon Tulum Cheese
Recep Kara (2015)

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