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Study Of Recellularized Human Acellular Arterial Matrix Repairs Porcine Biliary Segmental Defects

W. Liu, Sheng-ning Zhang, Zongqiang Hu, S. Feng, Zhen-Hui Li, S. Xiao, H. Wang, L. Li
Published 2019 · Medicine, Biology

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With the popularity of laparoscopic cholecystectomy, common bile duct injury has been reported more frequently. There is no perfect method for repairing porcine biliary segmental defects. After the decellularization of human arterial blood vessels, the cells were cultured with GFP+ (carry green fluorescent protein) porcine bile duct epithelial cells. The growth and proliferation of porcine bile duct epithelial cells on the human acellular arterial matrix (HAAM) were observed by hematoxylin–eosin (HE) staining, electron microscopy, and immunofluorescence. Then, the recellularized human acellular arterial matrix (RHAAM) was used to repair biliary segmental defects in the pig. The feasibility of it was detected by magnetic resonance cholangiopancreatography, liver function and blood routine changes, HE staining, immunofluorescence, real-time quantitative PCR (RT-qPCR), and western blot. After 4 weeks (w) of co-culture of HAAM and GFP+ porcine bile duct epithelial cells, GFP+ porcine bile duct epithelial cells grew stably, proliferated, and fused on HAAM. Bile was successfully drained into the duodenum without bile leakage or biliary obstruction. Immunofluorescence detection showed that GFP-positive bile duct cells could still be detected after GFP-containing bile duct cells were implanted into the acellular arterial matrix for 8 w. The implanted bile duct cells can successfully resist bile invasion and protect the acellular arterial matrix until the newborn bile duct is formed. The RHAAM can be used to repair biliary segmental defects in pigs, which provides a new idea for the clinical treatment of common bile duct injury.
This paper references
10.1002/lt.23696
Biliary complications adversely affect patient and graft survival after liver retransplantation.
C. Enestvedt (2013)
10.1016/j.biomaterials.2012.10.006
Emerging rules for inducing organ regeneration.
I. Yannas (2013)
10.1097/00000658-200112000-00006
Impaired Quality of Life 5 Years After Bile Duct Injury During Laparoscopic Cholecystectomy: A Prospective Analysis
D. Boerma (2001)
10.3109/10623320109063154
Vascular endothelial growth factor in porcine-derived extracellular matrix.
J. Hodde (2001)
10.1007/s00586-008-0745-3
Scaffolding in tissue engineering: general approaches and tissue-specific considerations
B. P. Chan (2008)
10.1002/bjs.1800840350
Incidence and nature of bile duct injuries following laparoscopic cholecystectomy: An audit of 5913 cases
E. Nilsson (1997)
10.1007/s00464-004-8942-6
Ten-year trend in the national volume of bile duct injuries requiring operative repair
J. P. Dolan (2004)
10.1002/hep.20704
AASLD practice guidelines: Evaluation of the patient for liver transplantation
K. Murray (2005)
10.1002/hep.20303
Immunolocalization of extracellular matrix components and integrins during mouse liver development
N. Shiojiri (2004)
Vascular endothelial growth factor in porcine-derived extracellular matrix. Endothelium
JP Hodde (2001)
10.1111/j.1432-0436.1992.tb00698.x
Ontogenesis of the murine hepatic extracellular matrix: an immunohistochemical study.
Z. Baloch (1992)
10.1016/J.SURG.2003.11.018
Management and outcome of patients with combined bile duct and hepatic arterial injuries after laparoscopic cholecystectomy.
S. Schmidt (2004)
10.1016/j.surg.2009.06.052
The impact of ischemic cholangiopathy in liver transplantation using donors after cardiac death: the untold story.
A. Skaro (2009)
10.1001/ARCHSURG.139.5.476
Long-term detrimental effect of bile duct injury on health-related quality of life.
Derek E. Moore (2004)
10.1002/LT
Are we reporting the same thing?
J. Perkins (2007)
10.1038/nm.4360
Reconstruction of the mouse extrahepatic biliary tree using primary human extrahepatic cholangiocyte organoids
F. Sampaziotis (2017)
10.1002/(SICI)1097-4644(19971215)67:4<478::AID-JCB6>3.0.CO;2-P
Identification of extractable growth factors from small intestinal submucosa
S. L. Voytik-Harbin (1997)
10.1080/003655202317316123
Laparoscopic Cholecystectomy: Bile Duct and Vascular Injuries: Management and Outcome
Ø. Mathisen (2002)
10.1001/JAMA.290.16.2168
Bile duct injury during cholecystectomy and survival in medicare beneficiaries.
D. Flum (2003)
Laparoscopic cholecystectomy: an analysis on 114,005 cases of United States series.
R. Vecchio (1998)
10.4240/wjgs.v4.i2.36
Long-term results of choledochoduodenostomy in benign biliary obstruction.
A. Malik (2012)
10.1001/archsurg.1993.01420170024002
Laparoscopic Cholecystectomy: A Statewide Experience
R. Orlando (1993)
10.1001/archsurg.1996.01430160040007
Bile duct injuries, 1989-1993. A statewide experience. Connecticut Laparoscopic Cholecystectomy Registry.
J. Russell (1996)
10.1152/physrev.00041.2008
Vascular extracellular matrix and arterial mechanics.
J. Wagenseil (2009)



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