Online citations, reference lists, and bibliographies.

Injectable Hyaluronic Acid Hydrogels Enriched With Platelet Lysate As A Cryostable Off-the-Shelf System For Cell-Based Therapies

Lisete S. Neves, Pedro S Babo, A I Gonçalves, Raquel Costa-Almeida, Sofia G. Caridade, João F Mano, Rui M A Domingues, Márcia T. Rodrigues, Rui Luís Reis, Manuela E. Gomes
Published 2017 · Medicine
Cite This
Download PDF
Analyze on Scholarcy
Cell-based regenerative medicine strategies hold a great potential to revolutionize the treatment of a large number of injuries with limited regenerative potential. However, the effectiveness of the simple injection of a cell suspension in a target site/tissue of action is often limited by the dispersion of cells toward other tissues, hindering their therapeutic action. Nevertheless, the development of a custom-made cell carrier that can perfectly fit a patient’s defect and be ready on demand is still a challenging task. The present study proposes the development of an off-the-shelf injectable cell delivery system combining a photocross-linkable hyaluronic acid (HA) matrix enriched with platelet lysate (PL) and human adipose tissue-derived stem cells (hASCs), which can be stored using standard cryopreservation methods and used when required. The obtained results indicate that the mechanical and viscoelastic properties of the system are improved in the presence of cells and no significant differences were identified between cell-laden hydrogels produced before or after cryopreservation. In PL-enriched hydrogels, cells tend to better recover from cryopreservation maintaining the values of cell viability and DNA content. Moreover, viable cells laden in our system and expressing stemness markers were detected after 21 days in culture. Altogether, the results obtained in this work demonstrate the potential of the developed strategy as an injectable cell delivery system for ready-to-use applications or as a cryopreserved product to be available on demand for cell-based therapies.
This paper references
Platelet Lysate-Loaded Photocrosslinkable Hyaluronic Acid Hydrogels for Periodontal Endogenous Regenerative Technology
Pedro S Babo (2017)
A clinically-feasible protocol for using human platelet lysate and mesenchymal stem cells in regenerative therapies.
Patrick Hans-Heinrich Warnke (2013)
relationship between solubility and stability
H Maity (2017)
Understanding the Role of Growth Factors in Modulating Stem Cell Tenogenesis
Ana I. Gonçalves (2013)
In situ forming degradable networks and their application in tissue engineering and drug delivery.
Kristi S Anseth (2002)
Human adipose tissue-derived SSEA-4 subpopulation multi-differentiation potential towards the endothelial and osteogenic lineages.
Silvia M. Mihaila (2013)
The effect of storage time on adipose-derived stem cell recovery from human Lipoaspirates. Cells Tissues Organs
PP Carvalho (2011)
Adipose tissue-derived mesenchymal stem cells and platelet-rich plasma: stem cell transplantation methods that enhance stemness
Morikuni Tobita (2015)
Development of Injectable Hyaluronic Acid/Cellulose Nanocrystals Bionanocomposite Hydrogels for Tissue Engineering Applications.
Rui M A Domingues (2015)
Dengue Virus Type 3 Adaptive Changes during Epidemics in São Jose de Rio Preto, Brazil, 2006–2007
Christian Julián Villabona-Arenas (2013)
Hyaluronan based porous nano-particles enriched with growth factors for the treatment of ulcers: a placebo-controlled study
Barbara Zavan (2009)
Adipose tissue-derived mesenchymal stem cells are more potent suppressors of dendritic cells differentiation compared to bone marrow-derived mesenchymal stem cells.
Ekaterina Ivanova-Todorova (2009)
Autologous Platelet-Rich Plasma: A Biological Supplement to Enhance Adipose-Derived Mesenchymal Stem Cell Expansion
AtashiFatemeh (2015)
Hyaluronic acid-based 3D culture model for in vitro testing of electrode biocompatibility.
Andrea F Jeffery (2014)
Photocrosslinkable Hyaluronan as a Scaffold for Articular Cartilage Repair
Dana L. Nettles (2004)
Current approaches and future perspectives on strategies for the development of personalized tissue engineering therapies
Lisete S. Neves (2016)
Autologous platelet-rich plasma: a biological supplement to enhance adipose-derived mesenchymal stem cell expansion.
Fatemeh Atashi (2015)
Cellular strategies to promote vascularisation in tissue engineering applications.
Raquel Costa-Almeida (2014)
Platelet lysate membranes as new autologous templates for tissue engineering applications
Pedro S Babo (2014)
Comparison of lignin derivatives as substrates for laccase-catalyzed scavenging of oxygen in coatings and films
Kristin Johansson (2014)
Biocompatibility of visible light-cured resin systems in prosthodontics.
Carol A. Lefebvre (1994)
Photocrosslinkable kappa-carrageenan hydrogels for tissue engineering applications.
Silvia M. Mihaila (2013)
Role of apoptosis-inducing factor (AIF) in programmed nuclear death during conjugation in Tetrahymena thermophila
Takahiko Akematsu (2009)
Hyaluronic acid modulates acute and chronic inflammation
Armando Ialenti (2005)
Photocrosslinkable polysaccharides for in situ hydrogel formation.
Kimberly A. Smeds (2001)
Platelet lysates promote mesenchymal stem cell expansion: a safety substitute for animal serum in cell-based therapy applications.
Christelle Doucet (2005)
Effect of platelet rich plasma on post cryopreservation viability, morphology and Regen
N Goei (2017)
Hyaluronic acid-based 3D culture model for in vitro testing of electrode biocompatibility. Biomacromolecules
AF Jeffery (2014)
Hyaluronan and its catabolic products in tissue injury and repair.
Paul James Noble (2002)
The individual-cell-based cryo-chip for the cryopreservation, manipulation and observation of spatially identifiable cells. II: Functional activity of cryopreserved cells
Elena Afrimzon (2010)
Phenotypic and Functional Characterization of Long-Term Cryopreserved Human Adipose-derived Stem Cells
Kar Wey Yong (2015)
Effect of Platelet Rich Plasma on Post Cryopreservation Viability, Morphology and Proliferation of Human Umbilical Cord Stem Cells
Noviyanti Goei (2015)
A fibrin/hyaluronic acid hydrogel for the delivery of mesenchymal stem cells and potential for articular cartilage repair
Timothy N Snyder (2014)
Chondrogenic potential of injectable κ-carrageenan hydrogel with encapsulated adipose stem cells for cartilage tissue-engineering applications.
Elena Geta Popa (2015)
Effects of pH and polyanions on the thermal stability of fibroblast growth factor 20.
Haihong Fan (2007)
Platelet lysate from whole blood-derived pooled platelet concentrates and apheresis-derived platelet concentrates for the isolation and expansion of human bone marrow mesenchymal stromal cells: production process, content and identification of active components
Natalie Fekete (2012)
Distinct Stem Cells Subpopulations Isolated from Human Adipose Tissue Exhibit Different Chondrogenic and Osteogenic Differentiation Potential
Tommaso Rada (2010)
Highly robust hydrogels via a fast, simple and cytocompatible dual crosslinking-based process.
Ana Maria S. Costa (2015)
Biodegradable electrospun nanofibers coated with platelet-rich plasma for cell adhesion and proliferation.
Luis Diaz-Gomez (2014)
Platelet Lysate Consisting of a Natural Repair Proteome Supports Human Mesenchymal Stem Cell Proliferation and Chromosomal Stability
Ruben J. Crespo-Diaz (2011)
Chitosan-chondroitin sulphate nanoparticles for controlled delivery of platelet lysates in bone regenerative medicine.
Vítor E Santo (2012)
An alternative approach to fabricating a meatus obturator prosthesis.
Suat Yaluğ (2003)
Effects of pH and arginine on the solubility and stability of a therapeutic protein (Fibroblast Growth Factor 20): relationship between solubility and stability.
Haripada Maity (2009)
Platelet lysate-loaded photocrosslinkable hyaluronic acid hydrogels exhibit Mitogenic and anti-microbial properties
P Babo (2016)
Comparison of immunomodulatory properties of mesenchymal stem cells derived from adult human tissues.
Keon Hee Yoo (2009)
Injectable glycosaminoglycan hydrogels for controlled release of human basic fibroblast growth factor.
Shenshen Cai (2005)
Hyaluronic acid-based polymers as cell carriers for tissue-engineered repair of bone and cartilage.
Luis A. Solchaga (1999)
Cryopreservation of cell laden natural origin hydrogels for cartilage regeneration strategies
Elena Geta Popa (2013)
Hyaluronic acid enhances proliferation and chondroitin sulfate synthesis in cultured chondrocytes embedded in collagen gels.
Kenzo Kawasaki (1999)
Effects of pH and polyanions on the thermal stability of fibroblast growth factor 20.Mol Pharm
HH Fan (2007)
Mesenchymal stem cell homing: the devil is in the details.
Jeffrey M. Karp (2009)
The Effect of Storage Time on Adipose-Derived Stem Cell Recovery from Human Lipoaspirates
Pedro P. Carvalho (2011)
Visible light photoinitiation of mesenchymal stem cell-laden bioresponsive hydrogels.
Chelsea S. Bahney (2011)
Modified Gellan Gum hydrogels with tunable physical and mechanical properties.
Daniela F. Coutinho (2010)
In situ crosslinkable hyaluronan hydrogels for tissue engineering.
Xiao Zheng Shu (2004)
Phase transfer-catalyzed synthesis of highly acrylated hyaluronan.
Jana Becher (2013)
Biopolymer-based hydrogels for cartilage tissue engineering.
Biji Balakrishnan (2011)

This paper is referenced by
Semantic Scholar Logo Some data provided by SemanticScholar